Gordonia phage Faith5x5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649V5F4|A0A649V5F4_9CAUD Antirepressor OS=Gordonia phage Faith5x5 OX=2656535 GN=37 PE=4 SV=1
MM1 pKa = 6.64NTYY4 pKa = 8.76MQDD7 pKa = 2.54IVTDD11 pKa = 3.99NRR13 pKa = 11.84CDD15 pKa = 3.21WSGLIVSQCAHH26 pKa = 6.07CRR28 pKa = 11.84GLISVLPNDD37 pKa = 3.57TCAGCGVGLVAGDD50 pKa = 3.7PNGEE54 pKa = 4.18NFCADD59 pKa = 3.62CTTWGAAA66 pKa = 3.43

Molecular weight: 6.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649V5L5|A0A649V5L5_9CAUD SsDNA binding protein OS=Gordonia phage Faith5x5 OX=2656535 GN=36 PE=4 SV=1
MM1 pKa = 6.37TAGYY5 pKa = 7.2FTLWAGEE12 pKa = 3.96RR13 pKa = 11.84VARR16 pKa = 11.84MDD18 pKa = 4.03EE19 pKa = 4.08LASAHH24 pKa = 5.88SAPVTVSPYY33 pKa = 8.33RR34 pKa = 11.84GRR36 pKa = 11.84MIAEE40 pKa = 3.88TRR42 pKa = 11.84PGKK45 pKa = 9.99RR46 pKa = 11.84DD47 pKa = 3.14LRR49 pKa = 11.84RR50 pKa = 11.84EE51 pKa = 3.74KK52 pKa = 10.85RR53 pKa = 11.84EE54 pKa = 3.7GVSAVRR60 pKa = 11.84GVLIGLALSVVMWAAIIALFVWLII84 pKa = 3.6

Molecular weight: 9.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

13425

37

1446

189.1

20.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.486 ± 0.426

0.946 ± 0.158

6.942 ± 0.297

6.153 ± 0.302

2.734 ± 0.178

8.931 ± 0.437

2.197 ± 0.256

5.147 ± 0.206

3.263 ± 0.196

7.181 ± 0.235

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.317 ± 0.11

2.927 ± 0.188

5.475 ± 0.215

3.456 ± 0.283

7.516 ± 0.37

5.408 ± 0.252

6.577 ± 0.363

7.017 ± 0.323

2.182 ± 0.15

2.145 ± 0.122
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski