Amino acid dipepetide frequency for Gordonia phage Faith5x5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.154AlaAla: 14.154 ± 1.167
0.894AlaCys: 0.894 ± 0.346
8.194AlaAsp: 8.194 ± 0.792
7.971AlaGlu: 7.971 ± 1.029
2.682AlaPhe: 2.682 ± 0.457
9.759AlaGly: 9.759 ± 0.751
2.16AlaHis: 2.16 ± 0.551
6.853AlaIle: 6.853 ± 0.697
4.023AlaLys: 4.023 ± 0.683
8.939AlaLeu: 8.939 ± 0.921
3.874AlaMet: 3.874 ± 0.581
4.097AlaAsn: 4.097 ± 0.511
4.693AlaPro: 4.693 ± 0.546
3.725AlaGln: 3.725 ± 0.515
8.492AlaArg: 8.492 ± 0.906
5.364AlaSer: 5.364 ± 0.736
7.524AlaThr: 7.524 ± 0.897
7.226AlaVal: 7.226 ± 0.798
1.788AlaTrp: 1.788 ± 0.312
2.384AlaTyr: 2.384 ± 0.376
0.0AlaXaa: 0.0 ± 0.0
Cys
1.49CysAla: 1.49 ± 0.459
0.074CysCys: 0.074 ± 0.071
0.968CysAsp: 0.968 ± 0.324
0.372CysGlu: 0.372 ± 0.157
0.0CysPhe: 0.0 ± 0.0
1.266CysGly: 1.266 ± 0.361
0.074CysHis: 0.074 ± 0.073
0.149CysIle: 0.149 ± 0.108
0.447CysLys: 0.447 ± 0.226
0.372CysLeu: 0.372 ± 0.171
0.223CysMet: 0.223 ± 0.144
0.372CysAsn: 0.372 ± 0.15
0.745CysPro: 0.745 ± 0.22
0.298CysGln: 0.298 ± 0.137
0.745CysArg: 0.745 ± 0.269
0.223CysSer: 0.223 ± 0.131
0.447CysThr: 0.447 ± 0.216
0.745CysVal: 0.745 ± 0.234
0.298CysTrp: 0.298 ± 0.134
0.149CysTyr: 0.149 ± 0.119
0.0CysXaa: 0.0 ± 0.0
Asp
7.226AspAla: 7.226 ± 0.769
0.745AspCys: 0.745 ± 0.238
4.693AspAsp: 4.693 ± 0.752
5.289AspGlu: 5.289 ± 0.701
2.086AspPhe: 2.086 ± 0.376
7.077AspGly: 7.077 ± 0.62
1.788AspHis: 1.788 ± 0.336
3.352AspIle: 3.352 ± 0.673
1.341AspLys: 1.341 ± 0.309
6.108AspLeu: 6.108 ± 0.65
1.117AspMet: 1.117 ± 0.246
2.16AspAsn: 2.16 ± 0.3
4.395AspPro: 4.395 ± 0.61
2.235AspGln: 2.235 ± 0.48
5.066AspArg: 5.066 ± 0.611
3.427AspSer: 3.427 ± 0.567
3.874AspThr: 3.874 ± 0.497
4.321AspVal: 4.321 ± 0.536
1.639AspTrp: 1.639 ± 0.4
1.49AspTyr: 1.49 ± 0.324
0.0AspXaa: 0.0 ± 0.0
Glu
8.045GluAla: 8.045 ± 0.763
0.745GluCys: 0.745 ± 0.241
3.65GluAsp: 3.65 ± 0.642
3.203GluGlu: 3.203 ± 0.472
2.235GluPhe: 2.235 ± 0.498
4.172GluGly: 4.172 ± 0.556
2.086GluHis: 2.086 ± 0.468
3.427GluIle: 3.427 ± 0.477
2.309GluLys: 2.309 ± 0.388
4.395GluLeu: 4.395 ± 0.57
1.564GluMet: 1.564 ± 0.337
1.192GluAsn: 1.192 ± 0.316
3.129GluPro: 3.129 ± 0.403
3.352GluGln: 3.352 ± 0.415
5.289GluArg: 5.289 ± 0.529
2.831GluSer: 2.831 ± 0.573
3.352GluThr: 3.352 ± 0.642
4.321GluVal: 4.321 ± 0.645
0.968GluTrp: 0.968 ± 0.263
1.266GluTyr: 1.266 ± 0.33
0.0GluXaa: 0.0 ± 0.0
Phe
3.278PheAla: 3.278 ± 0.595
0.447PheCys: 0.447 ± 0.178
2.235PheAsp: 2.235 ± 0.463
1.415PheGlu: 1.415 ± 0.314
0.819PhePhe: 0.819 ± 0.19
3.948PheGly: 3.948 ± 0.519
0.372PheHis: 0.372 ± 0.146
0.968PheIle: 0.968 ± 0.272
0.894PheLys: 0.894 ± 0.261
0.968PheLeu: 0.968 ± 0.298
0.372PheMet: 0.372 ± 0.143
0.968PheAsn: 0.968 ± 0.237
1.043PhePro: 1.043 ± 0.314
0.819PheGln: 0.819 ± 0.21
1.49PheArg: 1.49 ± 0.269
0.968PheSer: 0.968 ± 0.318
2.831PheThr: 2.831 ± 0.544
2.16PheVal: 2.16 ± 0.418
0.968PheTrp: 0.968 ± 0.31
0.372PheTyr: 0.372 ± 0.182
0.0PheXaa: 0.0 ± 0.0
Gly
8.716GlyAla: 8.716 ± 0.996
0.745GlyCys: 0.745 ± 0.316
6.779GlyAsp: 6.779 ± 0.63
5.289GlyGlu: 5.289 ± 0.552
2.235GlyPhe: 2.235 ± 0.454
8.716GlyGly: 8.716 ± 0.997
1.862GlyHis: 1.862 ± 0.368
4.768GlyIle: 4.768 ± 0.591
2.98GlyLys: 2.98 ± 0.493
7.002GlyLeu: 7.002 ± 0.841
2.086GlyMet: 2.086 ± 0.332
2.682GlyAsn: 2.682 ± 0.375
4.917GlyPro: 4.917 ± 0.463
2.682GlyGln: 2.682 ± 0.501
6.555GlyArg: 6.555 ± 0.603
4.768GlySer: 4.768 ± 0.64
5.587GlyThr: 5.587 ± 0.923
6.63GlyVal: 6.63 ± 0.701
1.564GlyTrp: 1.564 ± 0.382
2.756GlyTyr: 2.756 ± 0.429
0.0GlyXaa: 0.0 ± 0.0
His
1.713HisAla: 1.713 ± 0.328
0.372HisCys: 0.372 ± 0.173
1.49HisAsp: 1.49 ± 0.34
1.266HisGlu: 1.266 ± 0.419
0.447HisPhe: 0.447 ± 0.215
2.235HisGly: 2.235 ± 0.506
0.819HisHis: 0.819 ± 0.254
1.192HisIle: 1.192 ± 0.429
0.298HisLys: 0.298 ± 0.151
1.639HisLeu: 1.639 ± 0.389
0.596HisMet: 0.596 ± 0.287
0.372HisAsn: 0.372 ± 0.186
1.043HisPro: 1.043 ± 0.373
1.117HisGln: 1.117 ± 0.275
1.49HisArg: 1.49 ± 0.387
1.117HisSer: 1.117 ± 0.304
1.937HisThr: 1.937 ± 0.343
1.564HisVal: 1.564 ± 0.319
0.596HisTrp: 0.596 ± 0.213
0.67HisTyr: 0.67 ± 0.262
0.0HisXaa: 0.0 ± 0.0
Ile
7.673IleAla: 7.673 ± 0.771
0.149IleCys: 0.149 ± 0.119
4.097IleAsp: 4.097 ± 0.667
3.725IleGlu: 3.725 ± 0.481
1.415IlePhe: 1.415 ± 0.401
3.874IleGly: 3.874 ± 0.562
1.341IleHis: 1.341 ± 0.389
1.713IleIle: 1.713 ± 0.334
1.192IleLys: 1.192 ± 0.3
3.278IleLeu: 3.278 ± 0.52
0.447IleMet: 0.447 ± 0.165
1.415IleAsn: 1.415 ± 0.354
3.278IlePro: 3.278 ± 0.517
2.16IleGln: 2.16 ± 0.358
4.097IleArg: 4.097 ± 0.521
2.16IleSer: 2.16 ± 0.39
3.352IleThr: 3.352 ± 0.606
3.874IleVal: 3.874 ± 0.46
1.266IleTrp: 1.266 ± 0.329
0.968IleTyr: 0.968 ± 0.304
0.0IleXaa: 0.0 ± 0.0
Lys
5.066LysAla: 5.066 ± 0.645
0.223LysCys: 0.223 ± 0.172
2.086LysAsp: 2.086 ± 0.406
1.564LysGlu: 1.564 ± 0.356
0.745LysPhe: 0.745 ± 0.243
1.937LysGly: 1.937 ± 0.403
0.149LysHis: 0.149 ± 0.107
1.341LysIle: 1.341 ± 0.326
1.043LysLys: 1.043 ± 0.264
1.788LysLeu: 1.788 ± 0.389
0.67LysMet: 0.67 ± 0.216
0.745LysAsn: 0.745 ± 0.21
2.607LysPro: 2.607 ± 0.535
0.819LysGln: 0.819 ± 0.235
2.831LysArg: 2.831 ± 0.644
2.756LysSer: 2.756 ± 0.525
2.384LysThr: 2.384 ± 0.508
2.831LysVal: 2.831 ± 0.466
0.596LysTrp: 0.596 ± 0.224
0.447LysTyr: 0.447 ± 0.165
0.0LysXaa: 0.0 ± 0.0
Leu
8.194LeuAla: 8.194 ± 0.693
0.819LeuCys: 0.819 ± 0.233
5.587LeuAsp: 5.587 ± 0.509
4.544LeuGlu: 4.544 ± 0.521
1.937LeuPhe: 1.937 ± 0.521
7.151LeuGly: 7.151 ± 0.7
1.415LeuHis: 1.415 ± 0.472
4.246LeuIle: 4.246 ± 0.461
2.235LeuLys: 2.235 ± 0.535
4.544LeuLeu: 4.544 ± 0.674
1.192LeuMet: 1.192 ± 0.326
2.309LeuAsn: 2.309 ± 0.385
3.948LeuPro: 3.948 ± 0.61
1.639LeuGln: 1.639 ± 0.407
5.14LeuArg: 5.14 ± 0.731
3.948LeuSer: 3.948 ± 0.519
5.438LeuThr: 5.438 ± 0.713
4.917LeuVal: 4.917 ± 0.613
1.192LeuTrp: 1.192 ± 0.221
1.415LeuTyr: 1.415 ± 0.317
0.0LeuXaa: 0.0 ± 0.0
Met
2.235MetAla: 2.235 ± 0.544
0.298MetCys: 0.298 ± 0.155
1.192MetAsp: 1.192 ± 0.289
0.745MetGlu: 0.745 ± 0.239
0.745MetPhe: 0.745 ± 0.221
1.49MetGly: 1.49 ± 0.296
0.596MetHis: 0.596 ± 0.218
0.894MetIle: 0.894 ± 0.249
0.745MetLys: 0.745 ± 0.266
1.862MetLeu: 1.862 ± 0.401
0.372MetMet: 0.372 ± 0.165
1.341MetAsn: 1.341 ± 0.307
1.415MetPro: 1.415 ± 0.307
1.341MetGln: 1.341 ± 0.297
1.49MetArg: 1.49 ± 0.331
1.862MetSer: 1.862 ± 0.325
2.384MetThr: 2.384 ± 0.308
1.192MetVal: 1.192 ± 0.293
0.447MetTrp: 0.447 ± 0.187
0.521MetTyr: 0.521 ± 0.218
0.0MetXaa: 0.0 ± 0.0
Asn
3.501AsnAla: 3.501 ± 0.573
0.074AsnCys: 0.074 ± 0.09
1.415AsnAsp: 1.415 ± 0.276
1.415AsnGlu: 1.415 ± 0.358
0.894AsnPhe: 0.894 ± 0.251
3.65AsnGly: 3.65 ± 0.534
0.596AsnHis: 0.596 ± 0.21
0.819AsnIle: 0.819 ± 0.303
1.266AsnLys: 1.266 ± 0.326
2.16AsnLeu: 2.16 ± 0.343
1.117AsnMet: 1.117 ± 0.301
0.819AsnAsn: 0.819 ± 0.266
2.756AsnPro: 2.756 ± 0.435
1.117AsnGln: 1.117 ± 0.285
1.862AsnArg: 1.862 ± 0.356
1.341AsnSer: 1.341 ± 0.354
2.011AsnThr: 2.011 ± 0.358
1.564AsnVal: 1.564 ± 0.373
0.372AsnTrp: 0.372 ± 0.169
0.521AsnTyr: 0.521 ± 0.153
0.0AsnXaa: 0.0 ± 0.0
Pro
4.693ProAla: 4.693 ± 0.542
0.67ProCys: 0.67 ± 0.286
5.587ProAsp: 5.587 ± 0.676
3.948ProGlu: 3.948 ± 0.555
1.341ProPhe: 1.341 ± 0.29
5.215ProGly: 5.215 ± 0.516
1.49ProHis: 1.49 ± 0.364
2.756ProIle: 2.756 ± 0.406
2.16ProLys: 2.16 ± 0.453
3.352ProLeu: 3.352 ± 0.402
1.192ProMet: 1.192 ± 0.291
1.639ProAsn: 1.639 ± 0.43
3.129ProPro: 3.129 ± 0.604
1.713ProGln: 1.713 ± 0.276
2.98ProArg: 2.98 ± 0.488
4.023ProSer: 4.023 ± 0.517
2.607ProThr: 2.607 ± 0.443
3.501ProVal: 3.501 ± 0.457
1.713ProTrp: 1.713 ± 0.332
1.043ProTyr: 1.043 ± 0.295
0.0ProXaa: 0.0 ± 0.0
Gln
4.321GlnAla: 4.321 ± 0.612
0.372GlnCys: 0.372 ± 0.162
1.266GlnAsp: 1.266 ± 0.308
1.564GlnGlu: 1.564 ± 0.353
0.67GlnPhe: 0.67 ± 0.221
2.011GlnGly: 2.011 ± 0.517
0.968GlnHis: 0.968 ± 0.322
2.905GlnIle: 2.905 ± 0.429
1.415GlnLys: 1.415 ± 0.354
3.054GlnLeu: 3.054 ± 0.399
1.266GlnMet: 1.266 ± 0.314
0.894GlnAsn: 0.894 ± 0.246
1.788GlnPro: 1.788 ± 0.421
1.713GlnGln: 1.713 ± 0.409
3.129GlnArg: 3.129 ± 0.629
1.415GlnSer: 1.415 ± 0.298
2.384GlnThr: 2.384 ± 0.395
1.788GlnVal: 1.788 ± 0.402
0.67GlnTrp: 0.67 ± 0.185
0.968GlnTyr: 0.968 ± 0.313
0.0GlnXaa: 0.0 ± 0.0
Arg
8.716ArgAla: 8.716 ± 0.737
0.894ArgCys: 0.894 ± 0.276
4.842ArgAsp: 4.842 ± 0.553
5.289ArgGlu: 5.289 ± 0.72
2.607ArgPhe: 2.607 ± 0.41
4.768ArgGly: 4.768 ± 0.658
1.266ArgHis: 1.266 ± 0.33
3.948ArgIle: 3.948 ± 0.519
3.352ArgLys: 3.352 ± 0.469
5.885ArgLeu: 5.885 ± 0.61
1.862ArgMet: 1.862 ± 0.415
2.16ArgAsn: 2.16 ± 0.326
3.799ArgPro: 3.799 ± 0.55
2.235ArgGln: 2.235 ± 0.453
7.673ArgArg: 7.673 ± 1.085
3.278ArgSer: 3.278 ± 0.522
3.799ArgThr: 3.799 ± 0.463
4.619ArgVal: 4.619 ± 0.513
2.309ArgTrp: 2.309 ± 0.424
1.862ArgTyr: 1.862 ± 0.304
0.0ArgXaa: 0.0 ± 0.0
Ser
5.81SerAla: 5.81 ± 0.725
0.447SerCys: 0.447 ± 0.2
3.501SerAsp: 3.501 ± 0.576
3.352SerGlu: 3.352 ± 0.509
1.937SerPhe: 1.937 ± 0.34
6.034SerGly: 6.034 ± 0.761
1.043SerHis: 1.043 ± 0.311
2.905SerIle: 2.905 ± 0.499
1.49SerLys: 1.49 ± 0.364
3.501SerLeu: 3.501 ± 0.518
1.564SerMet: 1.564 ± 0.35
1.266SerAsn: 1.266 ± 0.285
2.831SerPro: 2.831 ± 0.527
1.713SerGln: 1.713 ± 0.361
3.129SerArg: 3.129 ± 0.555
2.98SerSer: 2.98 ± 0.705
3.501SerThr: 3.501 ± 0.887
4.619SerVal: 4.619 ± 0.592
1.341SerTrp: 1.341 ± 0.316
1.117SerTyr: 1.117 ± 0.233
0.0SerXaa: 0.0 ± 0.0
Thr
7.449ThrAla: 7.449 ± 0.838
0.596ThrCys: 0.596 ± 0.237
3.576ThrAsp: 3.576 ± 0.403
2.756ThrGlu: 2.756 ± 0.456
1.415ThrPhe: 1.415 ± 0.351
6.928ThrGly: 6.928 ± 0.959
1.341ThrHis: 1.341 ± 0.352
4.47ThrIle: 4.47 ± 0.611
1.639ThrLys: 1.639 ± 0.293
5.438ThrLeu: 5.438 ± 0.604
1.341ThrMet: 1.341 ± 0.335
2.086ThrAsn: 2.086 ± 0.437
3.948ThrPro: 3.948 ± 0.692
2.235ThrGln: 2.235 ± 0.456
4.246ThrArg: 4.246 ± 0.451
3.874ThrSer: 3.874 ± 0.6
4.47ThrThr: 4.47 ± 0.646
5.066ThrVal: 5.066 ± 0.719
1.639ThrTrp: 1.639 ± 0.376
1.266ThrTyr: 1.266 ± 0.284
0.0ThrXaa: 0.0 ± 0.0
Val
7.375ValAla: 7.375 ± 0.841
0.521ValCys: 0.521 ± 0.262
4.842ValAsp: 4.842 ± 0.596
5.662ValGlu: 5.662 ± 0.588
1.862ValPhe: 1.862 ± 0.339
5.885ValGly: 5.885 ± 0.944
1.415ValHis: 1.415 ± 0.243
3.054ValIle: 3.054 ± 0.407
2.235ValLys: 2.235 ± 0.578
4.395ValLeu: 4.395 ± 0.738
1.341ValMet: 1.341 ± 0.323
1.788ValAsn: 1.788 ± 0.431
3.129ValPro: 3.129 ± 0.474
1.415ValGln: 1.415 ± 0.422
5.662ValArg: 5.662 ± 0.668
4.693ValSer: 4.693 ± 0.802
5.662ValThr: 5.662 ± 0.722
6.332ValVal: 6.332 ± 0.811
1.117ValTrp: 1.117 ± 0.274
1.788ValTyr: 1.788 ± 0.462
0.0ValXaa: 0.0 ± 0.0
Trp
2.16TrpAla: 2.16 ± 0.395
0.223TrpCys: 0.223 ± 0.141
1.788TrpAsp: 1.788 ± 0.358
1.117TrpGlu: 1.117 ± 0.248
0.447TrpPhe: 0.447 ± 0.197
1.639TrpGly: 1.639 ± 0.297
0.819TrpHis: 0.819 ± 0.254
0.968TrpIle: 0.968 ± 0.258
0.745TrpLys: 0.745 ± 0.217
1.713TrpLeu: 1.713 ± 0.38
0.596TrpMet: 0.596 ± 0.193
0.521TrpAsn: 0.521 ± 0.171
1.043TrpPro: 1.043 ± 0.313
1.043TrpGln: 1.043 ± 0.246
1.937TrpArg: 1.937 ± 0.478
1.713TrpSer: 1.713 ± 0.383
0.968TrpThr: 0.968 ± 0.233
1.117TrpVal: 1.117 ± 0.273
0.968TrpTrp: 0.968 ± 0.332
0.298TrpTyr: 0.298 ± 0.157
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.054TyrAla: 3.054 ± 0.476
0.149TyrCys: 0.149 ± 0.103
1.639TyrAsp: 1.639 ± 0.287
1.043TyrGlu: 1.043 ± 0.274
0.819TyrPhe: 0.819 ± 0.243
1.564TyrGly: 1.564 ± 0.292
0.372TyrHis: 0.372 ± 0.172
0.745TyrIle: 0.745 ± 0.243
0.819TyrLys: 0.819 ± 0.279
1.415TyrLeu: 1.415 ± 0.261
0.298TyrMet: 0.298 ± 0.135
0.447TyrAsn: 0.447 ± 0.162
1.117TyrPro: 1.117 ± 0.346
1.192TyrGln: 1.192 ± 0.35
1.862TyrArg: 1.862 ± 0.347
1.341TyrSer: 1.341 ± 0.242
1.266TyrThr: 1.266 ± 0.319
1.788TyrVal: 1.788 ± 0.333
0.372TyrTrp: 0.372 ± 0.187
0.149TyrTyr: 0.149 ± 0.085
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 71 proteins (13425 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski