Stegodyphus mimosarum (African social velvet spider)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Chelicerata; Arachnida; Araneae; Araneomorphae; Entelegynae; Eresoidea; Eresidae; Stegodyphus

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 27009 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A087TSS8|A0A087TSS8_STEMI Uncharacterized protein (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_22046 PE=4 SV=1
MM1 pKa = 7.56LLKK4 pKa = 10.65NYY6 pKa = 9.69APPTEE11 pKa = 5.1GIHH14 pKa = 6.0HH15 pKa = 7.27AEE17 pKa = 4.36PNVTAYY23 pKa = 10.58FGDD26 pKa = 3.77KK27 pKa = 10.79NLGKK31 pKa = 8.67GTLYY35 pKa = 10.16ISEE38 pKa = 5.38SVLCWLTSSGDD49 pKa = 3.83GFSLSYY55 pKa = 9.92PSIYY59 pKa = 9.91IHH61 pKa = 7.55AISKK65 pKa = 10.03DD66 pKa = 3.76LNNFPYY72 pKa = 10.39EE73 pKa = 4.24CLYY76 pKa = 11.52LMIDD80 pKa = 3.51EE81 pKa = 5.77KK82 pKa = 10.72ITISEE87 pKa = 3.87VSEE90 pKa = 4.07IQDD93 pKa = 3.25EE94 pKa = 4.4VSEE97 pKa = 4.21DD98 pKa = 3.51QEE100 pKa = 4.99LRR102 pKa = 11.84SALNNLAVEE111 pKa = 4.31EE112 pKa = 4.61EE113 pKa = 4.38EE114 pKa = 5.45DD115 pKa = 4.74DD116 pKa = 4.01SLEE119 pKa = 4.05PAEE122 pKa = 4.14MHH124 pKa = 6.53FVPDD128 pKa = 4.01NKK130 pKa = 10.94NMLDD134 pKa = 3.5VMYY137 pKa = 10.48KK138 pKa = 10.41ALCDD142 pKa = 3.87CQALHH147 pKa = 7.26PDD149 pKa = 4.05SPTKK153 pKa = 10.96DD154 pKa = 3.43EE155 pKa = 5.62DD156 pKa = 4.43DD157 pKa = 4.49DD158 pKa = 5.57DD159 pKa = 6.15EE160 pKa = 5.81IVLPPISIQHH170 pKa = 6.03ANGVLEE176 pKa = 4.27EE177 pKa = 4.16MDD179 pKa = 5.2DD180 pKa = 4.04VVEE183 pKa = 5.32DD184 pKa = 4.38DD185 pKa = 4.19SDD187 pKa = 3.52MDD189 pKa = 3.74EE190 pKa = 4.52EE191 pKa = 4.21QFEE194 pKa = 4.45DD195 pKa = 4.47AEE197 pKa = 4.29QQ198 pKa = 3.61

Molecular weight: 22.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A087TGT9|A0A087TGT9_STEMI Zinc finger protein 595 (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_23760 PE=4 SV=1
MM1 pKa = 7.7PSLRR5 pKa = 11.84LTLRR9 pKa = 11.84KK10 pKa = 9.12PLIVSRR16 pKa = 11.84RR17 pKa = 11.84QQRR20 pKa = 11.84ASKK23 pKa = 9.1TVGRR27 pKa = 11.84TRR29 pKa = 11.84GRR31 pKa = 11.84RR32 pKa = 11.84KK33 pKa = 9.72RR34 pKa = 11.84RR35 pKa = 11.84ILCTHH40 pKa = 6.47NPSGHH45 pKa = 6.52HH46 pKa = 5.16FRR48 pKa = 11.84KK49 pKa = 9.74SRR51 pKa = 11.84LRR53 pKa = 11.84HH54 pKa = 5.17SSLVV58 pKa = 3.38

Molecular weight: 6.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

27006

3

27009

7704465

8

5000

285.3

32.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.908 ± 0.025

2.344 ± 0.019

5.246 ± 0.015

6.605 ± 0.027

4.062 ± 0.013

5.093 ± 0.025

2.778 ± 0.013

5.737 ± 0.014

6.669 ± 0.026

9.024 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.397 ± 0.008

4.902 ± 0.017

4.898 ± 0.02

4.08 ± 0.016

5.696 ± 0.026

8.628 ± 0.028

5.413 ± 0.017

6.144 ± 0.014

1.259 ± 0.008

3.101 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski