Motiliproteus sp. MSK22-1
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5085 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1R1MIG4|A0A1R1MIG4_9GAMM TonB_C domain-containing protein OS=Motiliproteus sp. MSK22-1 OX=1897630 GN=BGP75_02945 PE=3 SV=1
MM1 pKa = 8.12 RR2 pKa = 11.84 NRR4 pKa = 11.84 QSGFTIIEE12 pKa = 4.67 LIVVIALLGILSAVALPRR30 pKa = 11.84 FINVTAEE37 pKa = 3.75 AHH39 pKa = 5.96 DD40 pKa = 4.07 AAVEE44 pKa = 4.05 GAGAGFATGIALLKK58 pKa = 10.3 AQTVANGDD66 pKa = 3.65 LGTATGVDD74 pKa = 4.24 FDD76 pKa = 4.45 GSSMQVNASGFAVGASGAALSVTAASCFDD105 pKa = 3.1 IWTGILQGTGPVVSTTSGANIDD127 pKa = 4.16 YY128 pKa = 10.3 LVTAADD134 pKa = 3.9 PDD136 pKa = 3.88 CTYY139 pKa = 10.63 TYY141 pKa = 11.02 QNDD144 pKa = 3.48 SGQTIVYY151 pKa = 9.83 EE152 pKa = 4.14 SDD154 pKa = 3.0 TGNVTTTLL162 pKa = 3.55
Molecular weight: 16.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.726
IPC2_protein 3.808
IPC_protein 3.757
Toseland 3.541
ProMoST 3.961
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.745
Rodwell 3.592
Grimsley 3.465
Solomon 3.757
Lehninger 3.706
Nozaki 3.91
DTASelect 4.151
Thurlkill 3.63
EMBOSS 3.757
Sillero 3.897
Patrickios 0.769
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.787
Protein with the highest isoelectric point:
>tr|A0A1R1LLK5|A0A1R1LLK5_9GAMM Deacetylase OS=Motiliproteus sp. MSK22-1 OX=1897630 GN=BGP75_20920 PE=3 SV=1
MM1 pKa = 6.82 VQVHH5 pKa = 6.61 SDD7 pKa = 3.51 PPDD10 pKa = 3.43 YY11 pKa = 10.79 AIRR14 pKa = 11.84 RR15 pKa = 11.84 YY16 pKa = 10.16 EE17 pKa = 4.07 STCIAGYY24 pKa = 10.64 ASRR27 pKa = 11.84 FHH29 pKa = 7.83 ALIHH33 pKa = 5.87 EE34 pKa = 4.69 IWRR37 pKa = 11.84 KK38 pKa = 9.86 KK39 pKa = 8.92 LTPKK43 pKa = 10.36 FPALIDD49 pKa = 3.64 GNARR53 pKa = 11.84 AQRR56 pKa = 11.84 FFQRR60 pKa = 11.84 TRR62 pKa = 11.84 RR63 pKa = 11.84 AA64 pKa = 3.15
Molecular weight: 7.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.338
IPC2_protein 9.823
IPC_protein 10.774
Toseland 10.657
ProMoST 10.482
Dawson 10.789
Bjellqvist 10.57
Wikipedia 11.052
Rodwell 10.891
Grimsley 10.862
Solomon 10.935
Lehninger 10.891
Nozaki 10.657
DTASelect 10.555
Thurlkill 10.687
EMBOSS 11.082
Sillero 10.73
Patrickios 10.672
IPC_peptide 10.935
IPC2_peptide 9.692
IPC2.peptide.svr19 8.368
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5085
0
5085
1675512
41
6473
329.5
36.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.566 ± 0.032
1.082 ± 0.012
5.612 ± 0.04
6.378 ± 0.03
3.883 ± 0.025
7.266 ± 0.04
2.129 ± 0.016
6.165 ± 0.024
4.654 ± 0.036
10.904 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.448 ± 0.017
3.905 ± 0.024
4.169 ± 0.021
4.638 ± 0.032
5.297 ± 0.03
6.933 ± 0.029
5.081 ± 0.031
6.774 ± 0.033
1.244 ± 0.014
2.871 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here