Amino acid dipepetide frequency for Motiliproteus sp. MSK22-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.19AlaAla: 8.19 ± 0.088	0.976AlaCys: 0.976 ± 0.028	5.069AlaAsp: 5.069 ± 0.058	6.002AlaGlu: 6.002 ± 0.067	3.247AlaPhe: 3.247 ± 0.049	6.578AlaGly: 6.578 ± 0.079	1.565AlaHis: 1.565 ± 0.032	5.515AlaIle: 5.515 ± 0.066	3.872AlaLys: 3.872 ± 0.056	9.748AlaLeu: 9.748 ± 0.086	2.396AlaMet: 2.396 ± 0.04	3.001AlaAsn: 3.001 ± 0.04	2.934AlaPro: 2.934 ± 0.049	3.417AlaGln: 3.417 ± 0.054	4.246AlaArg: 4.246 ± 0.05	5.314AlaSer: 5.314 ± 0.055	4.218AlaThr: 4.218 ± 0.06	6.216AlaVal: 6.216 ± 0.061	0.953AlaTrp: 0.953 ± 0.026	2.201AlaTyr: 2.201 ± 0.034	0.0AlaXaa: 0.0 ± 0.0
Cys	0.81CysAla: 0.81 ± 0.022	0.196CysCys: 0.196 ± 0.012	0.606CysAsp: 0.606 ± 0.02	0.574CysGlu: 0.574 ± 0.018	0.499CysPhe: 0.499 ± 0.018	0.951CysGly: 0.951 ± 0.025	0.331CysHis: 0.331 ± 0.016	0.602CysIle: 0.602 ± 0.018	0.403CysLys: 0.403 ± 0.015	1.152CysLeu: 1.152 ± 0.032	0.232CysMet: 0.232 ± 0.011	0.378CysAsn: 0.378 ± 0.017	0.507CysPro: 0.507 ± 0.018	0.474CysGln: 0.474 ± 0.016	0.633CysArg: 0.633 ± 0.019	0.806CysSer: 0.806 ± 0.02	0.479CysThr: 0.479 ± 0.016	0.642CysVal: 0.642 ± 0.021	0.171CysTrp: 0.171 ± 0.012	0.375CysTyr: 0.375 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	4.164AspAla: 4.164 ± 0.067	0.602AspCys: 0.602 ± 0.017	3.007AspAsp: 3.007 ± 0.08	3.55AspGlu: 3.55 ± 0.052	2.304AspPhe: 2.304 ± 0.042	3.934AspGly: 3.934 ± 0.083	1.278AspHis: 1.278 ± 0.026	3.777AspIle: 3.777 ± 0.053	2.656AspLys: 2.656 ± 0.043	5.931AspLeu: 5.931 ± 0.064	1.281AspMet: 1.281 ± 0.025	2.341AspAsn: 2.341 ± 0.042	2.575AspPro: 2.575 ± 0.042	2.88AspGln: 2.88 ± 0.048	3.126AspArg: 3.126 ± 0.048	3.855AspSer: 3.855 ± 0.059	2.734AspThr: 2.734 ± 0.052	3.334AspVal: 3.334 ± 0.049	0.882AspTrp: 0.882 ± 0.025	1.911AspTyr: 1.911 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	5.635GluAla: 5.635 ± 0.069	0.493GluCys: 0.493 ± 0.018	3.123GluAsp: 3.123 ± 0.05	4.113GluGlu: 4.113 ± 0.062	2.243GluPhe: 2.243 ± 0.035	4.214GluGly: 4.214 ± 0.065	1.407GluHis: 1.407 ± 0.031	3.885GluIle: 3.885 ± 0.049	3.382GluLys: 3.382 ± 0.052	7.361GluLeu: 7.361 ± 0.073	1.607GluMet: 1.607 ± 0.034	2.492GluAsn: 2.492 ± 0.035	2.23GluPro: 2.23 ± 0.05	4.015GluGln: 4.015 ± 0.056	3.587GluArg: 3.587 ± 0.052	4.117GluSer: 4.117 ± 0.053	3.178GluThr: 3.178 ± 0.047	4.312GluVal: 4.312 ± 0.054	0.719GluTrp: 0.719 ± 0.021	1.67GluTyr: 1.67 ± 0.03	0.0GluXaa: 0.0 ± 0.0
Phe	3.013PheAla: 3.013 ± 0.042	0.54PheCys: 0.54 ± 0.02	2.601PheAsp: 2.601 ± 0.039	2.328PheGlu: 2.328 ± 0.043	1.658PhePhe: 1.658 ± 0.037	3.114PheGly: 3.114 ± 0.052	0.783PheHis: 0.783 ± 0.025	2.378PheIle: 2.378 ± 0.039	1.668PheLys: 1.668 ± 0.034	3.583PheLeu: 3.583 ± 0.058	0.919PheMet: 0.919 ± 0.024	1.682PheAsn: 1.682 ± 0.033	1.519PhePro: 1.519 ± 0.03	1.413PheGln: 1.413 ± 0.026	1.816PheArg: 1.816 ± 0.032	3.363PheSer: 3.363 ± 0.049	1.981PheThr: 1.981 ± 0.036	2.617PheVal: 2.617 ± 0.046	0.567PheTrp: 0.567 ± 0.021	1.291PheTyr: 1.291 ± 0.027	0.0PheXaa: 0.0 ± 0.0
Gly	5.765GlyAla: 5.765 ± 0.072	0.987GlyCys: 0.987 ± 0.028	3.924GlyAsp: 3.924 ± 0.105	4.269GlyGlu: 4.269 ± 0.061	3.338GlyPhe: 3.338 ± 0.051	5.33GlyGly: 5.33 ± 0.095	1.626GlyHis: 1.626 ± 0.035	4.911GlyIle: 4.911 ± 0.05	3.64GlyLys: 3.64 ± 0.049	7.801GlyLeu: 7.801 ± 0.073	2.146GlyMet: 2.146 ± 0.036	2.772GlyAsn: 2.772 ± 0.065	2.082GlyPro: 2.082 ± 0.036	2.954GlyGln: 2.954 ± 0.044	3.766GlyArg: 3.766 ± 0.053	4.739GlySer: 4.739 ± 0.066	3.683GlyThr: 3.683 ± 0.066	5.324GlyVal: 5.324 ± 0.059	1.044GlyTrp: 1.044 ± 0.027	2.56GlyTyr: 2.56 ± 0.04	0.0GlyXaa: 0.0 ± 0.0
His	1.463HisAla: 1.463 ± 0.033	0.35HisCys: 0.35 ± 0.016	0.976HisAsp: 0.976 ± 0.026	1.057HisGlu: 1.057 ± 0.027	0.988HisPhe: 0.988 ± 0.026	1.426HisGly: 1.426 ± 0.031	0.633HisHis: 0.633 ± 0.023	1.252HisIle: 1.252 ± 0.026	0.876HisLys: 0.876 ± 0.024	2.335HisLeu: 2.335 ± 0.038	0.498HisMet: 0.498 ± 0.018	0.808HisAsn: 0.808 ± 0.025	1.282HisPro: 1.282 ± 0.029	1.281HisGln: 1.281 ± 0.032	1.3HisArg: 1.3 ± 0.031	1.532HisSer: 1.532 ± 0.037	0.952HisThr: 0.952 ± 0.026	1.085HisVal: 1.085 ± 0.027	0.374HisTrp: 0.374 ± 0.014	0.819HisTyr: 0.819 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	5.784IleAla: 5.784 ± 0.072	0.747IleCys: 0.747 ± 0.022	4.052IleAsp: 4.052 ± 0.053	4.409IleGlu: 4.409 ± 0.062	2.072IlePhe: 2.072 ± 0.04	4.63IleGly: 4.63 ± 0.055	1.283IleHis: 1.283 ± 0.03	3.316IleIle: 3.316 ± 0.047	2.936IleLys: 2.936 ± 0.046	5.503IleLeu: 5.503 ± 0.069	1.111IleMet: 1.111 ± 0.03	2.853IleAsn: 2.853 ± 0.042	2.846IlePro: 2.846 ± 0.042	2.307IleGln: 2.307 ± 0.039	3.405IleArg: 3.405 ± 0.046	4.781IleSer: 4.781 ± 0.06	3.417IleThr: 3.417 ± 0.048	3.9IleVal: 3.9 ± 0.053	0.624IleTrp: 0.624 ± 0.023	1.674IleTyr: 1.674 ± 0.031	0.0IleXaa: 0.0 ± 0.0
Lys	4.334LysAla: 4.334 ± 0.062	0.3LysCys: 0.3 ± 0.015	2.492LysAsp: 2.492 ± 0.047	3.132LysGlu: 3.132 ± 0.05	1.228LysPhe: 1.228 ± 0.03	3.228LysGly: 3.228 ± 0.041	1.012LysHis: 1.012 ± 0.027	2.655LysIle: 2.655 ± 0.043	2.618LysLys: 2.618 ± 0.051	4.765LysLeu: 4.765 ± 0.066	1.143LysMet: 1.143 ± 0.028	1.953LysAsn: 1.953 ± 0.041	2.224LysPro: 2.224 ± 0.042	2.483LysGln: 2.483 ± 0.048	2.638LysArg: 2.638 ± 0.049	2.972LysSer: 2.972 ± 0.051	2.64LysThr: 2.64 ± 0.046	3.221LysVal: 3.221 ± 0.05	0.4LysTrp: 0.4 ± 0.017	1.106LysTyr: 1.106 ± 0.031	0.0LysXaa: 0.0 ± 0.0
Leu	9.921LeuAla: 9.921 ± 0.093	1.162LeuCys: 1.162 ± 0.028	6.082LeuAsp: 6.082 ± 0.078	6.95LeuGlu: 6.95 ± 0.08	4.38LeuPhe: 4.38 ± 0.056	7.413LeuGly: 7.413 ± 0.078	1.976LeuHis: 1.976 ± 0.036	6.85LeuIle: 6.85 ± 0.082	5.604LeuLys: 5.604 ± 0.064	11.859LeuLeu: 11.859 ± 0.131	2.755LeuMet: 2.755 ± 0.049	4.386LeuAsn: 4.386 ± 0.055	4.815LeuPro: 4.815 ± 0.061	4.362LeuGln: 4.362 ± 0.065	5.373LeuArg: 5.373 ± 0.073	8.384LeuSer: 8.384 ± 0.084	5.704LeuThr: 5.704 ± 0.059	7.183LeuVal: 7.183 ± 0.064	1.173LeuTrp: 1.173 ± 0.031	2.704LeuTyr: 2.704 ± 0.052	0.0LeuXaa: 0.0 ± 0.0
Met	2.448MetAla: 2.448 ± 0.044	0.178MetCys: 0.178 ± 0.01	1.343MetAsp: 1.343 ± 0.028	1.463MetGlu: 1.463 ± 0.031	0.738MetPhe: 0.738 ± 0.024	1.836MetGly: 1.836 ± 0.036	0.443MetHis: 0.443 ± 0.017	1.524MetIle: 1.524 ± 0.031	1.271MetLys: 1.271 ± 0.028	2.7MetLeu: 2.7 ± 0.046	0.658MetMet: 0.658 ± 0.023	1.05MetAsn: 1.05 ± 0.023	1.182MetPro: 1.182 ± 0.028	0.938MetGln: 0.938 ± 0.024	1.084MetArg: 1.084 ± 0.028	1.838MetSer: 1.838 ± 0.037	1.48MetThr: 1.48 ± 0.029	1.733MetVal: 1.733 ± 0.036	0.153MetTrp: 0.153 ± 0.01	0.417MetTyr: 0.417 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.025AsnAla: 3.025 ± 0.044	0.454AsnCys: 0.454 ± 0.016	2.114AsnAsp: 2.114 ± 0.05	2.127AsnGlu: 2.127 ± 0.038	1.345AsnPhe: 1.345 ± 0.027	2.877AsnGly: 2.877 ± 0.054	0.956AsnHis: 0.956 ± 0.024	2.434AsnIle: 2.434 ± 0.037	1.822AsnLys: 1.822 ± 0.039	3.962AsnLeu: 3.962 ± 0.053	0.865AsnMet: 0.865 ± 0.027	1.825AsnAsn: 1.825 ± 0.036	2.162AsnPro: 2.162 ± 0.036	1.989AsnGln: 1.989 ± 0.037	2.46AsnArg: 2.46 ± 0.042	2.737AsnSer: 2.737 ± 0.042	2.078AsnThr: 2.078 ± 0.038	2.053AsnVal: 2.053 ± 0.04	0.548AsnTrp: 0.548 ± 0.019	1.21AsnTyr: 1.21 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	3.524ProAla: 3.524 ± 0.049	0.371ProCys: 0.371 ± 0.015	2.756ProAsp: 2.756 ± 0.041	3.586ProGlu: 3.586 ± 0.057	1.693ProPhe: 1.693 ± 0.035	3.125ProGly: 3.125 ± 0.049	0.816ProHis: 0.816 ± 0.024	2.331ProIle: 2.331 ± 0.043	1.787ProLys: 1.787 ± 0.032	4.513ProLeu: 4.513 ± 0.055	0.98ProMet: 0.98 ± 0.026	1.39ProAsn: 1.39 ± 0.032	1.493ProPro: 1.493 ± 0.033	1.745ProGln: 1.745 ± 0.035	1.712ProArg: 1.712 ± 0.04	2.614ProSer: 2.614 ± 0.043	2.02ProThr: 2.02 ± 0.035	3.444ProVal: 3.444 ± 0.049	0.551ProTrp: 0.551 ± 0.021	1.236ProTyr: 1.236 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	4.235GlnAla: 4.235 ± 0.061	0.398GlnCys: 0.398 ± 0.015	1.959GlnAsp: 1.959 ± 0.032	2.562GlnGlu: 2.562 ± 0.041	1.568GlnPhe: 1.568 ± 0.032	3.231GlnGly: 3.231 ± 0.051	1.1GlnHis: 1.1 ± 0.026	2.719GlnIle: 2.719 ± 0.044	2.016GlnLys: 2.016 ± 0.039	5.895GlnLeu: 5.895 ± 0.077	1.089GlnMet: 1.089 ± 0.025	1.503GlnAsn: 1.503 ± 0.034	1.878GlnPro: 1.878 ± 0.038	3.456GlnGln: 3.456 ± 0.065	2.797GlnArg: 2.797 ± 0.048	2.937GlnSer: 2.937 ± 0.047	2.091GlnThr: 2.091 ± 0.035	3.145GlnVal: 3.145 ± 0.047	0.668GlnTrp: 0.668 ± 0.023	1.135GlnTyr: 1.135 ± 0.025	0.0GlnXaa: 0.0 ± 0.0
Arg	3.89ArgAla: 3.89 ± 0.048	0.584ArgCys: 0.584 ± 0.019	2.671ArgAsp: 2.671 ± 0.038	3.307ArgGlu: 3.307 ± 0.049	2.418ArgPhe: 2.418 ± 0.043	3.031ArgGly: 3.031 ± 0.046	1.241ArgHis: 1.241 ± 0.024	3.579ArgIle: 3.579 ± 0.049	2.606ArgLys: 2.606 ± 0.039	6.251ArgLeu: 6.251 ± 0.066	1.428ArgMet: 1.428 ± 0.029	2.078ArgAsn: 2.078 ± 0.037	1.951ArgPro: 1.951 ± 0.042	2.751ArgGln: 2.751 ± 0.05	2.941ArgArg: 2.941 ± 0.051	3.453ArgSer: 3.453 ± 0.052	2.477ArgThr: 2.477 ± 0.048	3.637ArgVal: 3.637 ± 0.052	0.774ArgTrp: 0.774 ± 0.024	1.903ArgTyr: 1.903 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	5.851SerAla: 5.851 ± 0.07	0.753SerCys: 0.753 ± 0.02	3.827SerAsp: 3.827 ± 0.058	4.337SerGlu: 4.337 ± 0.052	2.874SerPhe: 2.874 ± 0.036	5.702SerGly: 5.702 ± 0.079	1.548SerHis: 1.548 ± 0.031	4.223SerIle: 4.223 ± 0.053	2.945SerLys: 2.945 ± 0.049	7.482SerLeu: 7.482 ± 0.078	1.762SerMet: 1.762 ± 0.038	2.611SerAsn: 2.611 ± 0.044	2.809SerPro: 2.809 ± 0.049	2.972SerGln: 2.972 ± 0.047	3.604SerArg: 3.604 ± 0.04	4.909SerSer: 4.909 ± 0.077	3.479SerThr: 3.479 ± 0.049	4.562SerVal: 4.562 ± 0.055	0.948SerTrp: 0.948 ± 0.023	2.136SerTyr: 2.136 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	4.622ThrAla: 4.622 ± 0.065	0.446ThrCys: 0.446 ± 0.02	3.147ThrAsp: 3.147 ± 0.059	3.326ThrGlu: 3.326 ± 0.04	1.756ThrPhe: 1.756 ± 0.032	4.378ThrGly: 4.378 ± 0.065	1.071ThrHis: 1.071 ± 0.025	2.879ThrIle: 2.879 ± 0.045	1.745ThrLys: 1.745 ± 0.029	6.061ThrLeu: 6.061 ± 0.067	0.951ThrMet: 0.951 ± 0.025	1.724ThrAsn: 1.724 ± 0.04	2.696ThrPro: 2.696 ± 0.04	2.065ThrGln: 2.065 ± 0.035	2.476ThrArg: 2.476 ± 0.043	3.183ThrSer: 3.183 ± 0.057	2.707ThrThr: 2.707 ± 0.047	3.736ThrVal: 3.736 ± 0.05	0.523ThrTrp: 0.523 ± 0.019	1.318ThrTyr: 1.318 ± 0.036	0.0ThrXaa: 0.0 ± 0.0
Val	6.093ValAla: 6.093 ± 0.065	0.777ValCys: 0.777 ± 0.024	4.073ValAsp: 4.073 ± 0.054	4.585ValGlu: 4.585 ± 0.063	2.63ValPhe: 2.63 ± 0.042	4.661ValGly: 4.661 ± 0.062	1.212ValHis: 1.212 ± 0.026	4.565ValIle: 4.565 ± 0.058	3.022ValLys: 3.022 ± 0.046	7.117ValLeu: 7.117 ± 0.069	1.78ValMet: 1.78 ± 0.035	2.67ValAsn: 2.67 ± 0.04	2.661ValPro: 2.661 ± 0.044	2.313ValGln: 2.313 ± 0.043	3.34ValArg: 3.34 ± 0.048	4.958ValSer: 4.958 ± 0.068	3.693ValThr: 3.693 ± 0.054	5.154ValVal: 5.154 ± 0.063	0.729ValTrp: 0.729 ± 0.021	1.709ValTyr: 1.709 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	0.84TrpAla: 0.84 ± 0.026	0.144TrpCys: 0.144 ± 0.011	0.644TrpAsp: 0.644 ± 0.022	0.605TrpGlu: 0.605 ± 0.018	0.562TrpPhe: 0.562 ± 0.019	0.816TrpGly: 0.816 ± 0.026	0.302TrpHis: 0.302 ± 0.014	0.765TrpIle: 0.765 ± 0.023	0.568TrpLys: 0.568 ± 0.02	1.7TrpLeu: 1.7 ± 0.041	0.328TrpMet: 0.328 ± 0.015	0.516TrpAsn: 0.516 ± 0.018	0.481TrpPro: 0.481 ± 0.018	0.754TrpGln: 0.754 ± 0.024	0.721TrpArg: 0.721 ± 0.023	0.802TrpSer: 0.802 ± 0.023	0.51TrpThr: 0.51 ± 0.018	0.846TrpVal: 0.846 ± 0.023	0.182TrpTrp: 0.182 ± 0.011	0.351TrpTyr: 0.351 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.053TyrAla: 2.053 ± 0.037	0.363TyrCys: 0.363 ± 0.015	1.654TyrAsp: 1.654 ± 0.047	1.503TyrGlu: 1.503 ± 0.027	1.293TyrPhe: 1.293 ± 0.032	2.186TyrGly: 2.186 ± 0.051	0.704TyrHis: 0.704 ± 0.022	1.492TyrIle: 1.492 ± 0.032	1.108TyrLys: 1.108 ± 0.031	3.317TyrLeu: 3.317 ± 0.042	0.548TyrMet: 0.548 ± 0.019	1.01TyrAsn: 1.01 ± 0.029	1.357TyrPro: 1.357 ± 0.03	1.811TyrGln: 1.811 ± 0.035	1.947TyrArg: 1.947 ± 0.036	2.04TyrSer: 2.04 ± 0.039	1.29TyrThr: 1.29 ± 0.032	1.597TyrVal: 1.597 ± 0.03	0.451TyrTrp: 0.451 ± 0.018	0.988TyrTyr: 0.988 ± 0.029	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5085 proteins (1675512 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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