Acidithrix ferrooxidans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Acidimicrobiia; Acidimicrobiales; Acidimicrobiaceae; Acidithrix

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3548 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D8HCP9|A0A0D8HCP9_9ACTN Uncharacterized protein OS=Acidithrix ferrooxidans OX=1280514 GN=AXFE_34560 PE=4 SV=1
MM1 pKa = 7.66EE2 pKa = 5.59APDD5 pKa = 5.4LIGPDD10 pKa = 3.45EE11 pKa = 4.59FDD13 pKa = 3.04QVAVVLCLKK22 pKa = 10.77SLIVDD27 pKa = 3.45NLKK30 pKa = 10.87QIDD33 pKa = 4.27NFYY36 pKa = 11.62QMAFDD41 pKa = 3.57WLYY44 pKa = 11.35LVFVSYY50 pKa = 10.13FVEE53 pKa = 3.95IRR55 pKa = 11.84FVTLLCGNSFEE66 pKa = 4.96LNIVVSYY73 pKa = 11.35SFGKK77 pKa = 10.23EE78 pKa = 3.6ALTSS82 pKa = 3.62

Molecular weight: 9.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D8HCA3|A0A0D8HCA3_9ACTN Uncharacterized protein OS=Acidithrix ferrooxidans OX=1280514 GN=AXFE_35640 PE=4 SV=1
MM1 pKa = 8.0RR2 pKa = 11.84IAKK5 pKa = 9.52FAGIGAAALVAILAINWVLHH25 pKa = 5.91LMTSLFFGFVLVVVVVGAGFFVSKK49 pKa = 8.95TRR51 pKa = 11.84RR52 pKa = 11.84GSSKK56 pKa = 10.3GSRR59 pKa = 11.84NII61 pKa = 3.56

Molecular weight: 6.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3548

0

3548

982248

29

1864

276.8

30.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.307 ± 0.04

0.87 ± 0.015

5.357 ± 0.037

5.752 ± 0.046

3.878 ± 0.028

7.856 ± 0.041

1.939 ± 0.018

6.665 ± 0.034

4.072 ± 0.031

10.201 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.129 ± 0.016

3.541 ± 0.026

4.469 ± 0.027

3.022 ± 0.023

6.015 ± 0.04

8.218 ± 0.044

5.494 ± 0.043

7.479 ± 0.039

1.155 ± 0.016

2.581 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski