Treponema bryantii
Average proteome isoelectric point is 6.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3051 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H9AZM4|A0A1H9AZM4_9SPIO Uncharacterized protein OS=Treponema bryantii OX=163 GN=SAMN04487977_101527 PE=4 SV=1
MM1 pKa = 7.79 SNMYY5 pKa = 10.61 YY6 pKa = 10.31 YY7 pKa = 10.42 GTVTLYY13 pKa = 10.47 TDD15 pKa = 3.78 EE16 pKa = 5.85 GDD18 pKa = 3.04 IDD20 pKa = 3.91 VDD22 pKa = 3.77 VRR24 pKa = 11.84 AEE26 pKa = 3.83 ATGWYY31 pKa = 9.96 SSGDD35 pKa = 3.03 SWGYY39 pKa = 10.22 GCEE42 pKa = 4.11 PPDD45 pKa = 3.73 GDD47 pKa = 3.79 FTIDD51 pKa = 2.93 EE52 pKa = 4.34 VEE54 pKa = 4.16 YY55 pKa = 10.97 VKK57 pKa = 10.8 AYY59 pKa = 10.64 DD60 pKa = 5.06 DD61 pKa = 3.86 YY62 pKa = 11.99 SKK64 pKa = 11.41 DD65 pKa = 3.39 PVEE68 pKa = 4.72 ITDD71 pKa = 3.5 EE72 pKa = 4.04 MKK74 pKa = 10.63 KK75 pKa = 9.15 QVRR78 pKa = 11.84 EE79 pKa = 3.72 KK80 pKa = 10.93 LYY82 pKa = 10.05 DD83 pKa = 3.52 VEE85 pKa = 4.76 FKK87 pKa = 10.71 EE88 pKa = 5.9 HH89 pKa = 6.35 EE90 pKa = 4.39 FEE92 pKa = 5.07 PDD94 pKa = 2.72
Molecular weight: 10.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.757
IPC2_protein 3.859
IPC_protein 3.821
Toseland 3.617
ProMoST 3.935
Dawson 3.808
Bjellqvist 3.999
Wikipedia 3.732
Rodwell 3.656
Grimsley 3.528
Solomon 3.795
Lehninger 3.757
Nozaki 3.923
DTASelect 4.126
Thurlkill 3.668
EMBOSS 3.745
Sillero 3.948
Patrickios 0.693
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.862
Protein with the highest isoelectric point:
>tr|A0A1H9CJB4|A0A1H9CJB4_9SPIO 50S ribosomal protein L23 OS=Treponema bryantii OX=163 GN=rplW PE=3 SV=1
MM1 pKa = 7.64 AKK3 pKa = 10.13 KK4 pKa = 10.52 SLLIKK9 pKa = 10.27 AARR12 pKa = 11.84 KK13 pKa = 8.43 PKK15 pKa = 10.76 YY16 pKa = 7.22 EE17 pKa = 3.61 TRR19 pKa = 11.84 QYY21 pKa = 11.19 NRR23 pKa = 11.84 CQICGRR29 pKa = 11.84 PRR31 pKa = 11.84 GYY33 pKa = 10.38 LRR35 pKa = 11.84 KK36 pKa = 9.85 FKK38 pKa = 9.83 MCRR41 pKa = 11.84 ICFRR45 pKa = 11.84 KK46 pKa = 9.56 LASEE50 pKa = 4.25 GQLPGVTKK58 pKa = 10.74 SSWW61 pKa = 2.87
Molecular weight: 7.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.327
IPC2_protein 9.897
IPC_protein 10.526
Toseland 10.891
ProMoST 10.496
Dawson 10.965
Bjellqvist 10.643
Wikipedia 11.14
Rodwell 11.359
Grimsley 10.994
Solomon 11.067
Lehninger 11.038
Nozaki 10.877
DTASelect 10.628
Thurlkill 10.877
EMBOSS 11.272
Sillero 10.891
Patrickios 11.096
IPC_peptide 11.067
IPC2_peptide 9.765
IPC2.peptide.svr19 8.395
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3051
0
3051
1068725
29
4742
350.3
39.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.19 ± 0.052
1.424 ± 0.018
5.777 ± 0.039
7.037 ± 0.045
4.851 ± 0.041
6.544 ± 0.046
1.536 ± 0.019
7.772 ± 0.05
7.497 ± 0.036
8.715 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.449 ± 0.026
5.283 ± 0.045
3.438 ± 0.026
2.974 ± 0.023
3.722 ± 0.033
6.609 ± 0.047
5.7 ± 0.065
6.311 ± 0.036
1.052 ± 0.017
4.119 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here