Pseudonocardia autotrophica (Amycolata autotrophica) (Nocardia autotrophica)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Pseudonocardia

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6859 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y2MJB2|A0A1Y2MJB2_PSEAH 2-hydroxyhexa-2 4-dienoate hydratase OS=Pseudonocardia autotrophica OX=2074 GN=tesE_2 PE=4 SV=1
MM1 pKa = 7.49ALSPATMAGTAASVVLGAALLAGCGAAQANGLADD35 pKa = 4.85DD36 pKa = 4.61MAASAPQTYY45 pKa = 9.77SFTGAPVTVTVPDD58 pKa = 3.98GATGVTVTATGGSGGVGQGEE78 pKa = 4.47TSAGDD83 pKa = 3.85AACTAATGAAAGGMGGTVTGTSPVTAGQTITIAVGGSGSTDD124 pKa = 3.09GCDD127 pKa = 3.31GATNSAGGWGGDD139 pKa = 3.55GGTGGAGLDD148 pKa = 4.66GYY150 pKa = 11.6SSDD153 pKa = 3.1NWTSGGGGGASTVDD167 pKa = 3.41VAGTTIWAGGGGGGGAKK184 pKa = 10.11GIEE187 pKa = 4.22AGGSGGSGAQNGGPGHH203 pKa = 7.06AGHH206 pKa = 6.84GPGAGKK212 pKa = 10.14EE213 pKa = 4.48GPSEE217 pKa = 4.47DD218 pKa = 3.63MPGPAGGSGGHH229 pKa = 6.39GGWDD233 pKa = 3.5GGDD236 pKa = 3.33GGGGGGGRR244 pKa = 11.84TGGRR248 pKa = 11.84EE249 pKa = 4.09GEE251 pKa = 4.24GGQFGGGGGGGGGAGGTWADD271 pKa = 3.27GRR273 pKa = 11.84VVSGISYY280 pKa = 7.92GTATAAGAGSITLTWQNN297 pKa = 3.12

Molecular weight: 25.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y2MQG0|A0A1Y2MQG0_PSEAH D-inositol 3-phosphate glycosyltransferase OS=Pseudonocardia autotrophica OX=2074 GN=mshA_3 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 10.06GRR40 pKa = 11.84TNVSAA45 pKa = 4.51

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6859

0

6859

2184090

30

8584

318.4

33.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.131 ± 0.049

0.759 ± 0.009

6.361 ± 0.026

5.34 ± 0.026

2.558 ± 0.02

10.021 ± 0.034

2.213 ± 0.016

3.273 ± 0.024

1.227 ± 0.019

10.337 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.66 ± 0.013

1.484 ± 0.016

6.48 ± 0.034

2.441 ± 0.019

8.536 ± 0.039

4.865 ± 0.028

6.074 ± 0.021

9.116 ± 0.03

1.412 ± 0.011

1.713 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski