Botrytis porri botybirnavirus 1
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I3PN88|I3PN88_9VIRU Uncharacterized protein OS=Botrytis porri botybirnavirus 1 OX=1918014 PE=4 SV=1
MM1 pKa = 7.34 KK2 pKa = 10.48 FKK4 pKa = 10.42 MPYY7 pKa = 7.87 NTQTASSAQEE17 pKa = 3.89 DD18 pKa = 3.69 NRR20 pKa = 11.84 VEE22 pKa = 4.15 NVAKK26 pKa = 10.47 SAPQNINLPAEE37 pKa = 4.35 LWALVSSSLQSYY49 pKa = 10.34 DD50 pKa = 3.29 CAVVSKK56 pKa = 10.46 CVGVSSHH63 pKa = 6.64 PLVVAMMSRR72 pKa = 11.84 AADD75 pKa = 3.93 EE76 pKa = 4.34 YY77 pKa = 11.24 KK78 pKa = 10.73 YY79 pKa = 9.95 PCPAARR85 pKa = 11.84 RR86 pKa = 11.84 VYY88 pKa = 10.55 AVPCGCKK95 pKa = 9.86 AFMRR99 pKa = 11.84 STVGHH104 pKa = 6.32 LVVKK108 pKa = 10.14 KK109 pKa = 10.73 DD110 pKa = 3.55 EE111 pKa = 4.32 VGSFICCAYY120 pKa = 10.68 NGTRR124 pKa = 11.84 SCRR127 pKa = 11.84 CKK129 pKa = 10.22 SGSMSWVTTQDD140 pKa = 2.8 RR141 pKa = 11.84 EE142 pKa = 3.9 EE143 pKa = 4.57 ALRR146 pKa = 11.84 QDD148 pKa = 4.68 LLATYY153 pKa = 10.27 ARR155 pKa = 11.84 QTKK158 pKa = 9.02 GRR160 pKa = 11.84 YY161 pKa = 8.65 GPNPTEE167 pKa = 4.35 SEE169 pKa = 4.11 MEE171 pKa = 4.0 EE172 pKa = 4.46 KK173 pKa = 10.64 DD174 pKa = 4.45 RR175 pKa = 11.84 EE176 pKa = 4.2 DD177 pKa = 4.61 RR178 pKa = 11.84 DD179 pKa = 3.48 LADD182 pKa = 2.84 WRR184 pKa = 11.84 DD185 pKa = 3.51 YY186 pKa = 11.53 LRR188 pKa = 11.84 YY189 pKa = 9.44 CAEE192 pKa = 4.05 KK193 pKa = 10.79 VVLEE197 pKa = 4.41 EE198 pKa = 4.28 DD199 pKa = 3.67 LCLKK203 pKa = 10.73 ALFFSRR209 pKa = 11.84 EE210 pKa = 4.12 LTVLGPKK217 pKa = 10.24 GADD220 pKa = 3.42 EE221 pKa = 5.06 PGSDD225 pKa = 4.35 PVPPPPSGPVLDD237 pKa = 4.72 RR238 pKa = 11.84 EE239 pKa = 4.67 TVGDD243 pKa = 4.07 DD244 pKa = 3.51 TVPDD248 pKa = 3.92 QVTGPDD254 pKa = 3.88 LPVAAPTPVNPGDD267 pKa = 3.38 EE268 pKa = 4.03 AAAEE272 pKa = 4.08 QEE274 pKa = 4.15 NSGNKK279 pKa = 9.24 YY280 pKa = 10.77 EE281 pKa = 4.37 NVLSNEE287 pKa = 3.94 RR288 pKa = 11.84 SGQPVEE294 pKa = 4.37 EE295 pKa = 4.83 PEE297 pKa = 4.14 HH298 pKa = 6.72 AGWLSWLNDD307 pKa = 3.48 EE308 pKa = 5.49 DD309 pKa = 4.23 DD310 pKa = 3.98 TMFDD314 pKa = 3.89 RR315 pKa = 11.84 YY316 pKa = 10.5 AGTIMRR322 pKa = 11.84 SARR325 pKa = 11.84 HH326 pKa = 5.7 KK327 pKa = 9.98 AWEE330 pKa = 4.16 PALQGHH336 pKa = 6.43 WNNVFTIGGDD346 pKa = 2.86 IMRR349 pKa = 11.84 DD350 pKa = 3.14 VKK352 pKa = 11.06 AHH354 pKa = 6.31 HH355 pKa = 6.44 KK356 pKa = 8.01 TVAMSLSPHH365 pKa = 4.86 VTWGYY370 pKa = 11.7 GEE372 pKa = 4.29 GTPDD376 pKa = 3.36 ATITKK381 pKa = 9.84 AGYY384 pKa = 7.77 IDD386 pKa = 4.03 SYY388 pKa = 11.48 EE389 pKa = 4.19 VVQCTTDD396 pKa = 2.88 RR397 pKa = 11.84 RR398 pKa = 11.84 TVMIPFARR406 pKa = 11.84 DD407 pKa = 3.46 TIAYY411 pKa = 7.19 NVPFGMSVYY420 pKa = 10.68 NRR422 pKa = 11.84 AQPFNSTASVTPLSDD437 pKa = 2.77 ILSAVEE443 pKa = 5.04 DD444 pKa = 3.82 KK445 pKa = 11.41 ALTSAYY451 pKa = 10.38 NSLILTSSSKK461 pKa = 10.98 GNNHH465 pKa = 6.49 IALGCTMYY473 pKa = 9.93 MRR475 pKa = 11.84 LMALEE480 pKa = 4.0 MLAEE484 pKa = 4.18 QGAATYY490 pKa = 10.4 IDD492 pKa = 3.86 LTAEE496 pKa = 3.91 GFNRR500 pKa = 11.84 FLLPVEE506 pKa = 5.45 DD507 pKa = 5.53 GDD509 pKa = 4.07 DD510 pKa = 3.54 APIRR514 pKa = 11.84 KK515 pKa = 8.71 VARR518 pKa = 11.84 SVVAHH523 pKa = 5.75 QPYY526 pKa = 11.33 NMITLPDD533 pKa = 3.45 QSADD537 pKa = 3.06 SDD539 pKa = 4.7 AILMYY544 pKa = 10.6 YY545 pKa = 10.38 LAGNSRR551 pKa = 11.84 ITTYY555 pKa = 9.45 VTAGPGAPDD564 pKa = 3.85 LAVHH568 pKa = 5.59 STIDD572 pKa = 3.6 LYY574 pKa = 11.66 VPEE577 pKa = 4.73 QRR579 pKa = 11.84 FTLCPLNGSEE589 pKa = 5.39 LIEE592 pKa = 4.63 EE593 pKa = 4.64 RR594 pKa = 11.84 DD595 pKa = 3.63 SDD597 pKa = 4.06 DD598 pKa = 4.86 DD599 pKa = 4.29 GAFKK603 pKa = 10.89 VDD605 pKa = 3.64 AYY607 pKa = 10.36 RR608 pKa = 11.84 ARR610 pKa = 11.84 SLVSRR615 pKa = 11.84 YY616 pKa = 7.62 LTAHH620 pKa = 6.41 NLWQQFPVMRR630 pKa = 11.84 AFAWALLAHH639 pKa = 7.4 PATSVNIQYY648 pKa = 7.76 PAPMHH653 pKa = 5.78 TADD656 pKa = 4.07 LQLNLFPNPASIEE669 pKa = 4.01 HH670 pKa = 6.52 RR671 pKa = 11.84 GVILGEE677 pKa = 4.23 RR678 pKa = 11.84 DD679 pKa = 3.49 HH680 pKa = 5.95 QTSLHH685 pKa = 6.06 ASVVTAARR693 pKa = 11.84 MAEE696 pKa = 4.07 EE697 pKa = 4.42 VLTDD701 pKa = 4.1 SIVSTVVEE709 pKa = 4.23 AGIEE713 pKa = 4.22 YY714 pKa = 8.75 TNPAYY719 pKa = 9.78 TGTVEE724 pKa = 4.29 QEE726 pKa = 4.1 LGRR729 pKa = 11.84 HH730 pKa = 6.13 LYY732 pKa = 9.94 HH733 pKa = 7.3 GYY735 pKa = 10.34 AHH737 pKa = 7.91 LIMPIVEE744 pKa = 4.52 KK745 pKa = 10.92 LLGDD749 pKa = 4.17 TFPEE753 pKa = 4.27 LTQYY757 pKa = 11.17 LADD760 pKa = 3.55 SVLALEE766 pKa = 4.89 HH767 pKa = 6.46 CTTGRR772 pKa = 11.84 DD773 pKa = 3.28 LDD775 pKa = 3.88 RR776 pKa = 11.84 KK777 pKa = 9.9 KK778 pKa = 10.89 FRR780 pKa = 11.84 ASSYY784 pKa = 10.5 LCMEE788 pKa = 4.64 EE789 pKa = 4.22 TPEE792 pKa = 4.47 GEE794 pKa = 3.91 GWQVVFSEE802 pKa = 4.36 SRR804 pKa = 11.84 RR805 pKa = 11.84 KK806 pKa = 9.87 QILQQEE812 pKa = 4.91 YY813 pKa = 8.16 CTEE816 pKa = 4.16 RR817 pKa = 11.84 EE818 pKa = 3.91 QLTCLWLTQKK828 pKa = 10.09 VAQEE832 pKa = 3.96 NVGAVTYY839 pKa = 8.16 YY840 pKa = 9.37 QHH842 pKa = 7.34 EE843 pKa = 4.38 ILPSEE848 pKa = 4.57 SVSDD852 pKa = 3.87 TPVVRR857 pKa = 11.84 GWLGKK862 pKa = 10.02 NRR864 pKa = 11.84 LDD866 pKa = 4.02 YY867 pKa = 11.34 LRR869 pKa = 11.84 GAPILWGTSTTAVAYY884 pKa = 9.03 KK885 pKa = 10.42 HH886 pKa = 6.25 SPQRR890 pKa = 11.84 VKK892 pKa = 10.8 EE893 pKa = 4.09 FQLEE897 pKa = 4.38 SNEE900 pKa = 4.26 LGPPTGLFAQLYY912 pKa = 6.37 EE913 pKa = 4.61 KK914 pKa = 10.62 RR915 pKa = 11.84 MQVNSEE921 pKa = 3.55 AMIRR925 pKa = 11.84 EE926 pKa = 4.29 DD927 pKa = 4.4 EE928 pKa = 4.31 EE929 pKa = 4.61 ALGTASHH936 pKa = 6.74 RR937 pKa = 11.84 EE938 pKa = 4.02 EE939 pKa = 5.07 KK940 pKa = 10.54 GWAPKK945 pKa = 10.43 LEE947 pKa = 4.56 PKK949 pKa = 10.26 RR950 pKa = 11.84 EE951 pKa = 4.0 EE952 pKa = 3.99 KK953 pKa = 10.53 KK954 pKa = 10.4 EE955 pKa = 3.68 EE956 pKa = 4.03 VVRR959 pKa = 11.84 VRR961 pKa = 11.84 PTATKK966 pKa = 8.07 KK967 pKa = 8.85 TKK969 pKa = 9.75 PVRR972 pKa = 11.84 QQVPVLAVKK981 pKa = 10.24 AVPSPDD987 pKa = 3.26 KK988 pKa = 11.25 EE989 pKa = 4.06 GFLPVKK995 pKa = 10.43 GGARR999 pKa = 11.84 PTSVQVTRR1007 pKa = 11.84 MTTMPMGKK1015 pKa = 9.44 SPMKK1019 pKa = 10.17 VANSTPKK1026 pKa = 9.95 VRR1028 pKa = 11.84 RR1029 pKa = 11.84 TLSGGYY1035 pKa = 9.43 KK1036 pKa = 10.09 KK1037 pKa = 8.81 PTPWYY1042 pKa = 8.94 LRR1044 pKa = 11.84 ASSRR1048 pKa = 11.84 EE1049 pKa = 3.97 EE1050 pKa = 3.55 EE1051 pKa = 3.88 EE1052 pKa = 4.18 AALKK1056 pKa = 10.56 KK1057 pKa = 10.48 GAEE1060 pKa = 3.99 ARR1062 pKa = 11.84 KK1063 pKa = 9.79 SFEE1066 pKa = 4.14 SRR1068 pKa = 11.84 PLSLGRR1074 pKa = 11.84 LRR1076 pKa = 11.84 ILLEE1080 pKa = 4.11 KK1081 pKa = 9.8 EE1082 pKa = 3.63 QTSAEE1087 pKa = 3.97 DD1088 pKa = 2.99 RR1089 pKa = 11.84 KK1090 pKa = 11.01 EE1091 pKa = 3.12 IDD1093 pKa = 2.81 RR1094 pKa = 11.84 YY1095 pKa = 10.1 YY1096 pKa = 11.18 DD1097 pKa = 3.13 RR1098 pKa = 11.84 GMDD1101 pKa = 3.63 AALKK1105 pKa = 9.29 RR1106 pKa = 11.84 APYY1109 pKa = 8.17 TAGVMWSKK1117 pKa = 10.68 RR1118 pKa = 11.84 QIIAKK1123 pKa = 9.07 NKK1125 pKa = 8.13 TRR1127 pKa = 11.84 IAHH1130 pKa = 6.97 LKK1132 pKa = 8.33 TAMLNGAYY1140 pKa = 9.83 GKK1142 pKa = 9.32 NAWMVTLGLWIMTNTMTDD1160 pKa = 2.6 NCFEE1164 pKa = 4.43 DD1165 pKa = 4.82 LIKK1168 pKa = 10.81 EE1169 pKa = 4.67 GILKK1173 pKa = 8.98 TEE1175 pKa = 3.91 YY1176 pKa = 10.97 SDD1178 pKa = 4.68 WNSKK1182 pKa = 7.08 WASYY1186 pKa = 11.3 NDD1188 pKa = 3.58 IIRR1191 pKa = 11.84 NKK1193 pKa = 7.09 WAKK1196 pKa = 10.84 GEE1198 pKa = 4.12 FQHH1201 pKa = 6.9 TPDD1204 pKa = 5.64 DD1205 pKa = 4.27 LVQCLYY1211 pKa = 9.4 IANMVGRR1218 pKa = 11.84 PHH1220 pKa = 7.48 RR1221 pKa = 11.84 EE1222 pKa = 3.8 CDD1224 pKa = 2.87 WDD1226 pKa = 4.44 DD1227 pKa = 3.59 EE1228 pKa = 4.94 VKK1230 pKa = 10.68 KK1231 pKa = 9.66 RR1232 pKa = 11.84 TRR1234 pKa = 11.84 NVGEE1238 pKa = 3.72 IKK1240 pKa = 10.14 IARR1243 pKa = 11.84 NGKK1246 pKa = 8.07 MVPADD1251 pKa = 3.51 EE1252 pKa = 4.58 SQLEE1256 pKa = 4.14 EE1257 pKa = 5.21 EE1258 pKa = 4.58 ILQMLLKK1265 pKa = 10.23 EE1266 pKa = 3.95 GSIRR1270 pKa = 11.84 VKK1272 pKa = 10.44 KK1273 pKa = 10.33 PKK1275 pKa = 9.82 TFEE1278 pKa = 3.61 QFYY1281 pKa = 10.02 SSRR1284 pKa = 11.84 GNWMIKK1290 pKa = 10.09 GSASGEE1296 pKa = 3.69 RR1297 pKa = 11.84 MLISEE1302 pKa = 4.47 YY1303 pKa = 10.87 KK1304 pKa = 10.39 EE1305 pKa = 3.36 IVGQVKK1311 pKa = 10.36 GLGVEE1316 pKa = 3.98 LRR1318 pKa = 11.84 EE1319 pKa = 4.18 RR1320 pKa = 11.84 ATKK1323 pKa = 10.19 TDD1325 pKa = 3.01 VAEE1328 pKa = 4.22 YY1329 pKa = 10.75 VSAQAVLALLDD1340 pKa = 4.21 DD1341 pKa = 4.25 MAIHH1345 pKa = 7.09 LAKK1348 pKa = 10.65 AHH1350 pKa = 5.9 TKK1352 pKa = 9.94 GNEE1355 pKa = 3.84 HH1356 pKa = 5.99 GKK1358 pKa = 9.69 VRR1360 pKa = 11.84 AIYY1363 pKa = 9.57 GSLYY1367 pKa = 9.44 AHH1369 pKa = 6.28 YY1370 pKa = 10.54 VLGSFWSTYY1379 pKa = 11.06 LEE1381 pKa = 4.24 DD1382 pKa = 4.06 TVTLASASMNKK1393 pKa = 10.1 DD1394 pKa = 2.89 NSRR1397 pKa = 11.84 LIAEE1401 pKa = 4.81 TMQRR1405 pKa = 11.84 AISCRR1410 pKa = 11.84 SGRR1413 pKa = 11.84 WIVCLDD1419 pKa = 3.57 YY1420 pKa = 11.68 ADD1422 pKa = 5.83 FNAQHH1427 pKa = 6.62 SGMAQRR1433 pKa = 11.84 SVIRR1437 pKa = 11.84 TLYY1440 pKa = 10.07 KK1441 pKa = 7.3 WAKK1444 pKa = 9.27 MMGFQPTEE1452 pKa = 3.61 EE1453 pKa = 4.19 FNRR1456 pKa = 11.84 ISEE1459 pKa = 4.48 WYY1461 pKa = 9.75 ADD1463 pKa = 3.44 SFTNQWFQRR1472 pKa = 11.84 PDD1474 pKa = 2.79 NKK1476 pKa = 10.16 QWVRR1480 pKa = 11.84 AVSGMFSGVRR1490 pKa = 11.84 QTTLINTILNLTYY1503 pKa = 10.7 HH1504 pKa = 7.01 HH1505 pKa = 7.09 IAMKK1509 pKa = 10.33 NCYY1512 pKa = 9.9 NLGQKK1517 pKa = 8.55 VEE1519 pKa = 4.16 CLQTYY1524 pKa = 10.2 VLGDD1528 pKa = 3.94 DD1529 pKa = 3.75 GWVEE1533 pKa = 4.22 FSTKK1537 pKa = 10.34 EE1538 pKa = 3.75 EE1539 pKa = 4.07 AEE1541 pKa = 4.39 MYY1543 pKa = 10.2 VVAARR1548 pKa = 11.84 MGGMEE1553 pKa = 4.17 INAIKK1558 pKa = 10.35 QLIGQGKK1565 pKa = 9.47 GEE1567 pKa = 4.35 YY1568 pKa = 9.95 LRR1570 pKa = 11.84 LIYY1573 pKa = 10.74 DD1574 pKa = 2.68 VDD1576 pKa = 3.52 GRR1578 pKa = 11.84 VRR1580 pKa = 11.84 GCPVRR1585 pKa = 11.84 SLASFVHH1592 pKa = 6.32 GNVEE1596 pKa = 4.05 NKK1598 pKa = 9.08 QASVGQQRR1606 pKa = 11.84 ITEE1609 pKa = 4.4 MYY1611 pKa = 9.8 SQACMLVRR1619 pKa = 11.84 RR1620 pKa = 11.84 GLDD1623 pKa = 3.12 PARR1626 pKa = 11.84 WQKK1629 pKa = 10.43 VFEE1632 pKa = 4.77 DD1633 pKa = 3.57 LAIYY1637 pKa = 9.4 EE1638 pKa = 4.2 IGYY1641 pKa = 5.01 TTQVSRR1647 pKa = 11.84 GQCLKK1652 pKa = 10.56 YY1653 pKa = 10.61 LYY1655 pKa = 10.28 GSKK1658 pKa = 10.41 RR1659 pKa = 11.84 SGGLGLMPLNTATDD1673 pKa = 3.85 RR1674 pKa = 11.84 VAKK1677 pKa = 10.46 LPVEE1681 pKa = 4.23 AEE1683 pKa = 3.97 EE1684 pKa = 4.13 AAEE1687 pKa = 4.12 EE1688 pKa = 4.1 VDD1690 pKa = 3.88 VAQILADD1697 pKa = 3.65 SLVEE1701 pKa = 3.77 GRR1703 pKa = 11.84 VANKK1707 pKa = 9.57 FKK1709 pKa = 10.99 ASSDD1713 pKa = 3.68 YY1714 pKa = 11.35 VGTLEE1719 pKa = 4.87 RR1720 pKa = 11.84 EE1721 pKa = 4.09 YY1722 pKa = 11.38 GVVWKK1727 pKa = 10.78 HH1728 pKa = 5.12 NGKK1731 pKa = 9.53 IKK1733 pKa = 10.02 ATAQVAASNLVEE1745 pKa = 5.16 GITNVGAEE1753 pKa = 4.09 HH1754 pKa = 6.89 EE1755 pKa = 4.42 VLKK1758 pKa = 10.56 MRR1760 pKa = 11.84 VLGAQLEE1767 pKa = 4.39 KK1768 pKa = 11.07 AKK1770 pKa = 10.09 WLEE1773 pKa = 3.68 AMNWDD1778 pKa = 3.43 RR1779 pKa = 11.84 WNNGPLIGPEE1789 pKa = 4.08 NIEE1792 pKa = 3.66 KK1793 pKa = 10.01 QYY1795 pKa = 11.24 RR1796 pKa = 11.84 KK1797 pKa = 9.8 IRR1799 pKa = 11.84 VSEE1802 pKa = 4.4 DD1803 pKa = 2.94 KK1804 pKa = 11.11 LLSTVTKK1811 pKa = 9.75 VAKK1814 pKa = 9.83 LAHH1817 pKa = 6.24 LMSAEE1822 pKa = 4.12 SVEE1825 pKa = 4.01 AVQMKK1830 pKa = 9.8 IAADD1834 pKa = 3.74 LDD1836 pKa = 4.01 VSVTAVKK1843 pKa = 10.49 HH1844 pKa = 4.89 SFKK1847 pKa = 10.6 SASVLRR1853 pKa = 11.84 GEE1855 pKa = 4.53 QIDD1858 pKa = 4.64 YY1859 pKa = 10.6 IPKK1862 pKa = 9.61 PVMAPEE1868 pKa = 4.27 LEE1870 pKa = 4.96 GIYY1873 pKa = 7.83 TQWLTVNNNDD1883 pKa = 3.7 SQQMSIPRR1891 pKa = 11.84 WIADD1895 pKa = 3.27 YY1896 pKa = 9.38 TAVLRR1901 pKa = 11.84 YY1902 pKa = 9.43
Molecular weight: 213.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.965
IPC2_protein 5.995
IPC_protein 6.046
Toseland 6.351
ProMoST 6.415
Dawson 6.326
Bjellqvist 6.338
Wikipedia 6.326
Rodwell 6.313
Grimsley 6.478
Solomon 6.326
Lehninger 6.313
Nozaki 6.59
DTASelect 6.766
Thurlkill 6.781
EMBOSS 6.751
Sillero 6.693
Patrickios 4.215
IPC_peptide 6.338
IPC2_peptide 6.605
IPC2.peptide.svr19 6.628
Protein with the highest isoelectric point:
>tr|I3PN88|I3PN88_9VIRU Uncharacterized protein OS=Botrytis porri botybirnavirus 1 OX=1918014 PE=4 SV=1
MM1 pKa = 7.34 KK2 pKa = 10.48 FKK4 pKa = 10.42 MPYY7 pKa = 7.87 NTQTASSAQEE17 pKa = 3.89 DD18 pKa = 3.69 NRR20 pKa = 11.84 VEE22 pKa = 4.14 NVAKK26 pKa = 10.53 SSPRR30 pKa = 11.84 KK31 pKa = 6.97 TAQPKK36 pKa = 8.8 SKK38 pKa = 10.11 KK39 pKa = 10.0 VKK41 pKa = 9.92 AGKK44 pKa = 8.84 KK45 pKa = 9.59 AKK47 pKa = 9.91 RR48 pKa = 11.84 ALAASKK54 pKa = 10.55 SHH56 pKa = 6.76 FDD58 pKa = 4.51 GYY60 pKa = 11.1 CHH62 pKa = 6.22 MAFFEE67 pKa = 4.44 EE68 pKa = 4.4 KK69 pKa = 10.24 DD70 pKa = 3.32 RR71 pKa = 11.84 AAAASNLLAWPKK83 pKa = 10.57 AGAIKK88 pKa = 10.34 KK89 pKa = 9.67 YY90 pKa = 10.42 VEE92 pKa = 4.12 EE93 pKa = 4.17 NKK95 pKa = 10.02 ISLSTEE101 pKa = 3.73 RR102 pKa = 11.84 VSALLLLAPEE112 pKa = 4.79 DD113 pKa = 3.62 VDD115 pKa = 5.02 AVVAYY120 pKa = 9.72 HH121 pKa = 6.69 IVPGTLFPAEE131 pKa = 4.38 VLYY134 pKa = 11.56 SMADD138 pKa = 3.36 NVVVGGGVSGTNLAAYY154 pKa = 8.84 IVAYY158 pKa = 10.51 ALMLCTYY165 pKa = 9.69 VCIAIARR172 pKa = 11.84 GATSDD177 pKa = 3.51 KK178 pKa = 10.55 EE179 pKa = 4.46 SNEE182 pKa = 3.6 RR183 pKa = 11.84 RR184 pKa = 11.84 LATLGPKK191 pKa = 9.8 GFKK194 pKa = 8.84 PNKK197 pKa = 8.63 MPTKK201 pKa = 10.45 AEE203 pKa = 3.97 RR204 pKa = 11.84 AGAAAAGIYY213 pKa = 9.97 KK214 pKa = 10.17 DD215 pKa = 3.87 AGGMTPVVQTLDD227 pKa = 3.19 NSATLWEE234 pKa = 5.81 AITNWKK240 pKa = 10.0 VNDD243 pKa = 3.82 RR244 pKa = 11.84 LAQSEE249 pKa = 4.66 AYY251 pKa = 9.82 GRR253 pKa = 11.84 VIIQPSATRR262 pKa = 11.84 LISMTGALMAKK273 pKa = 8.82 MGKK276 pKa = 9.87 FKK278 pKa = 10.37 FTDD281 pKa = 3.2 TFSQLKK287 pKa = 7.97 PTLDD291 pKa = 3.81 PVDD294 pKa = 4.38 VALCWGYY301 pKa = 11.04 NLDD304 pKa = 3.94 KK305 pKa = 11.17 LPTLRR310 pKa = 11.84 VGTAYY315 pKa = 9.66 TDD317 pKa = 3.27 KK318 pKa = 11.06 HH319 pKa = 6.63 SVNLWSYY326 pKa = 10.83 RR327 pKa = 11.84 PTSAQSRR334 pKa = 11.84 IKK336 pKa = 10.64 AVNTGIGYY344 pKa = 7.41 TSPRR348 pKa = 11.84 VSANQHH354 pKa = 5.0 IAGVTDD360 pKa = 3.58 NADD363 pKa = 3.57 KK364 pKa = 11.21 LVDD367 pKa = 3.71 AKK369 pKa = 11.19 DD370 pKa = 3.89 YY371 pKa = 11.13 IVLGDD376 pKa = 3.63 MRR378 pKa = 11.84 KK379 pKa = 9.38 KK380 pKa = 10.87 VPSEE384 pKa = 3.68 WYY386 pKa = 9.73 KK387 pKa = 11.07 AGNVKK392 pKa = 10.46 AQFGDD397 pKa = 3.36 NHH399 pKa = 7.87 IGMLVQWLIMLWSVRR414 pKa = 11.84 QAEE417 pKa = 4.5 EE418 pKa = 3.83 QGSTCHH424 pKa = 5.87 VRR426 pKa = 11.84 ASSSAAEE433 pKa = 4.36 KK434 pKa = 9.31 YY435 pKa = 8.55 TLNVVPAKK443 pKa = 9.24 NTNEE447 pKa = 4.01 TTEE450 pKa = 4.1 VKK452 pKa = 10.36 IMEE455 pKa = 4.69 AVAHH459 pKa = 5.26 AWEE462 pKa = 4.25 WAIKK466 pKa = 10.1 AKK468 pKa = 10.5 PFDD471 pKa = 4.03 LVLPGDD477 pKa = 3.94 SANPIDD483 pKa = 5.08 IIHH486 pKa = 6.14 MMFLCGMLQDD496 pKa = 3.97 TTKK499 pKa = 10.41 FRR501 pKa = 11.84 YY502 pKa = 9.79 NKK504 pKa = 8.72 TDD506 pKa = 3.49 LSIVPTYY513 pKa = 9.11 YY514 pKa = 10.38 TYY516 pKa = 9.6 QTDD519 pKa = 2.63 IRR521 pKa = 11.84 EE522 pKa = 4.13 YY523 pKa = 11.22 LRR525 pKa = 11.84 IPLCNSLKK533 pKa = 10.69 ALSLVDD539 pKa = 4.23 LSEE542 pKa = 4.16 NEE544 pKa = 4.09 YY545 pKa = 11.39 LMTSGGLEE553 pKa = 4.45 CIIIDD558 pKa = 3.51 YY559 pKa = 8.85 VRR561 pKa = 11.84 RR562 pKa = 11.84 MGLQDD567 pKa = 3.48 QLHH570 pKa = 5.82 NAQLIACSLIVSDD583 pKa = 5.4 ISNSDD588 pKa = 3.55 SNLMGGIPKK597 pKa = 9.58 PNHH600 pKa = 4.44 VQEE603 pKa = 4.27 YY604 pKa = 8.92 EE605 pKa = 3.92 LWLSTRR611 pKa = 11.84 AQGRR615 pKa = 11.84 GTTLTLMQGEE625 pKa = 4.52 SFALLFALLQIQRR638 pKa = 11.84 GVLIDD643 pKa = 3.4 VVAAKK648 pKa = 9.81 MIEE651 pKa = 4.08 RR652 pKa = 11.84 VQSKK656 pKa = 8.65 GIPMISIAGEE666 pKa = 3.88 RR667 pKa = 11.84 EE668 pKa = 3.63 AGIFLSEE675 pKa = 4.16 VLGSGASQLMSAWGSHH691 pKa = 3.68 MFGYY695 pKa = 10.59 VSAEE699 pKa = 3.85 QEE701 pKa = 4.39 KK702 pKa = 10.66 LLHH705 pKa = 6.36 TDD707 pKa = 2.6 AVMLTRR713 pKa = 11.84 YY714 pKa = 10.16 LEE716 pKa = 4.16 EE717 pKa = 4.09 TFTTGRR723 pKa = 11.84 LRR725 pKa = 11.84 PTSCMLAGTTIEE737 pKa = 4.18 TGAMSLIWDD746 pKa = 3.41 EE747 pKa = 3.91 ATRR750 pKa = 11.84 VKK752 pKa = 10.44 EE753 pKa = 3.94 ATSVFVTKK761 pKa = 10.18 PVARR765 pKa = 11.84 MLSMLYY771 pKa = 9.9 SEE773 pKa = 5.4 KK774 pKa = 10.95 KK775 pKa = 10.64 NIFDD779 pKa = 3.87 TEE781 pKa = 4.35 GLFMFNFTDD790 pKa = 4.31 FGTKK794 pKa = 10.0 NRR796 pKa = 11.84 DD797 pKa = 2.89 QRR799 pKa = 11.84 MTVSSTGLRR808 pKa = 11.84 RR809 pKa = 11.84 VGTIKK814 pKa = 10.61 GAPKK818 pKa = 8.87 MLYY821 pKa = 9.68 VGTVPAHH828 pKa = 4.86 NTMAXRR834 pKa = 11.84 LAHH837 pKa = 6.46 TGTGPQLAAHH847 pKa = 6.18 YY848 pKa = 10.02 EE849 pKa = 4.02 PFFDD853 pKa = 5.27 QDD855 pKa = 2.97 AAANTVQTDD864 pKa = 3.86 LPIKK868 pKa = 10.35 AGPSGNTKK876 pKa = 7.03 MHH878 pKa = 6.78 KK879 pKa = 10.15 DD880 pKa = 3.46 EE881 pKa = 4.89 PVSVSQIKK889 pKa = 10.32 RR890 pKa = 11.84 PTGQRR895 pKa = 11.84 TRR897 pKa = 11.84 KK898 pKa = 9.5 NSWVEE903 pKa = 3.68 FAKK906 pKa = 10.87 DD907 pKa = 3.31 ALATAGRR914 pKa = 11.84 MVEE917 pKa = 4.3 GRR919 pKa = 11.84 SKK921 pKa = 10.9 DD922 pKa = 3.79 LQPVTPPRR930 pKa = 11.84 KK931 pKa = 9.76 DD932 pKa = 2.9 IYY934 pKa = 10.43 VEE936 pKa = 3.9 QMVYY940 pKa = 10.59 AQAPMYY946 pKa = 8.95 TRR948 pKa = 11.84 TMHH951 pKa = 6.83 PGSAYY956 pKa = 10.0 DD957 pKa = 3.59 AYY959 pKa = 11.05 HH960 pKa = 6.48 NGVFDD965 pKa = 3.97 TLEE968 pKa = 4.16 EE969 pKa = 4.12 LASEE973 pKa = 4.57 ASARR977 pKa = 11.84 AQAAIDD983 pKa = 3.61 TMSEE987 pKa = 3.98 PEE989 pKa = 3.98 EE990 pKa = 4.18 NKK992 pKa = 10.27 ASEE995 pKa = 4.72 IITANPARR1003 pKa = 11.84 INKK1006 pKa = 8.91 HH1007 pKa = 4.28 VNQMYY1012 pKa = 10.33 AVADD1016 pKa = 4.0 FEE1018 pKa = 4.44 EE1019 pKa = 5.26 YY1020 pKa = 10.36 EE1021 pKa = 4.35 VVIAEE1026 pKa = 3.83 EE1027 pKa = 4.34 DD1028 pKa = 3.32 QRR1030 pKa = 11.84 GLYY1033 pKa = 10.27 DD1034 pKa = 4.1 HH1035 pKa = 7.11 IKK1037 pKa = 9.63 VVPTIGDD1044 pKa = 3.45 GRR1046 pKa = 11.84 CGVRR1050 pKa = 11.84 ALHH1053 pKa = 6.13 AQMVAKK1059 pKa = 10.43 GDD1061 pKa = 4.01 MISLTEE1067 pKa = 4.07 VYY1069 pKa = 9.8 EE1070 pKa = 4.63 VEE1072 pKa = 4.18 AQVCGLEE1079 pKa = 3.94 EE1080 pKa = 3.93 RR1081 pKa = 11.84 RR1082 pKa = 11.84 VAKK1085 pKa = 9.67 QIWATDD1091 pKa = 3.41 EE1092 pKa = 4.01 VLALVARR1099 pKa = 11.84 QYY1101 pKa = 11.4 GYY1103 pKa = 11.19 SLVMYY1108 pKa = 10.01 QEE1110 pKa = 5.15 SNDD1113 pKa = 3.66 EE1114 pKa = 4.79 DD1115 pKa = 3.74 PTIDD1119 pKa = 3.39 KK1120 pKa = 10.66 VRR1122 pKa = 11.84 VIATGHH1128 pKa = 5.07 NRR1130 pKa = 11.84 YY1131 pKa = 9.92 LEE1133 pKa = 4.3 ILSQPTHH1140 pKa = 4.59 WTAMKK1145 pKa = 9.31 WDD1147 pKa = 3.85 AEE1149 pKa = 3.66 IDD1151 pKa = 3.94 YY1152 pKa = 11.09 ADD1154 pKa = 4.25 EE1155 pKa = 3.97 EE1156 pKa = 4.64 TLRR1159 pKa = 11.84 EE1160 pKa = 4.06 TIHH1163 pKa = 7.12 AYY1165 pKa = 10.0 LAEE1168 pKa = 4.61 FGEE1171 pKa = 4.54 RR1172 pKa = 11.84 AVPGAYY1178 pKa = 9.85 SSEE1181 pKa = 4.02 KK1182 pKa = 9.85 GASGGEE1188 pKa = 3.79 KK1189 pKa = 10.57 DD1190 pKa = 4.99 KK1191 pKa = 11.64 VLFPAKK1197 pKa = 9.44 TPKK1200 pKa = 9.67 AQKK1203 pKa = 9.71 PFRR1206 pKa = 11.84 STPLSAADD1214 pKa = 4.36 DD1215 pKa = 4.52 EE1216 pKa = 5.0 IIAAIEE1222 pKa = 3.86 KK1223 pKa = 7.95 STPKK1227 pKa = 10.37 SKK1229 pKa = 9.94 QHH1231 pKa = 6.18 SAADD1235 pKa = 3.51 HH1236 pKa = 5.68 YY1237 pKa = 11.17 KK1238 pKa = 8.98 WEE1240 pKa = 4.14 NKK1242 pKa = 9.72 ARR1244 pKa = 11.84 VAVVGHH1250 pKa = 5.29 PHH1252 pKa = 5.67 RR1253 pKa = 11.84 TGMTEE1258 pKa = 3.95 FQNGAIGVSRR1268 pKa = 11.84 YY1269 pKa = 8.14 GARR1272 pKa = 11.84 TWDD1275 pKa = 3.35 QYY1277 pKa = 10.58 VEE1279 pKa = 4.3 EE1280 pKa = 4.49 VAAVHH1285 pKa = 6.57 GGISNKK1291 pKa = 9.41 ISEE1294 pKa = 4.53 YY1295 pKa = 9.92 STAEE1299 pKa = 3.72 LDD1301 pKa = 3.6 GTAISPSTLHH1311 pKa = 6.79 LGVEE1315 pKa = 4.76 LSLTTEE1321 pKa = 3.9 RR1322 pKa = 11.84 MIKK1325 pKa = 10.2 HH1326 pKa = 6.58 LAQGADD1332 pKa = 2.98 DD1333 pKa = 5.26 HH1334 pKa = 7.97 YY1335 pKa = 11.98 YY1336 pKa = 10.91 EE1337 pKa = 4.61 DD1338 pKa = 4.01 TLNRR1342 pKa = 11.84 FCDD1345 pKa = 3.37 HH1346 pKa = 6.59 YY1347 pKa = 10.54 MIKK1350 pKa = 10.61 PEE1352 pKa = 5.44 DD1353 pKa = 3.86 RR1354 pKa = 11.84 DD1355 pKa = 3.66 HH1356 pKa = 7.13 LKK1358 pKa = 10.69 NSHH1361 pKa = 6.56 PRR1363 pKa = 11.84 VPVLKK1368 pKa = 10.25 ALAGFAEE1375 pKa = 4.89 LPFAVIEE1382 pKa = 4.08 VDD1384 pKa = 3.22 RR1385 pKa = 11.84 SSEE1388 pKa = 3.9 VMRR1391 pKa = 11.84 LVRR1394 pKa = 11.84 LHH1396 pKa = 6.88 KK1397 pKa = 10.27 YY1398 pKa = 9.65 VPQHH1402 pKa = 5.1 TARR1405 pKa = 11.84 SRR1407 pKa = 11.84 YY1408 pKa = 9.01 QPTFLLYY1415 pKa = 10.97 GNNLFYY1421 pKa = 11.22 LRR1423 pKa = 11.84 MGEE1426 pKa = 4.12 FSDD1429 pKa = 4.13 PNAITRR1435 pKa = 11.84 KK1436 pKa = 9.56 EE1437 pKa = 4.08 GIAAYY1442 pKa = 9.93 LADD1445 pKa = 3.51 VRR1447 pKa = 11.84 TRR1449 pKa = 11.84 YY1450 pKa = 10.2 ADD1452 pKa = 3.15 SSYY1455 pKa = 11.64 AAAQSTKK1462 pKa = 10.42 SRR1464 pKa = 11.84 EE1465 pKa = 3.66 EE1466 pKa = 3.34 WFAAVDD1472 pKa = 3.61 NKK1474 pKa = 10.11 LAEE1477 pKa = 4.17 MGIEE1481 pKa = 3.67 PSAYY1485 pKa = 9.53 KK1486 pKa = 10.12 SVCSVAEE1493 pKa = 3.47 MDD1495 pKa = 4.17 MIFKK1499 pKa = 10.6 KK1500 pKa = 10.16 GTGEE1504 pKa = 5.3 GEE1506 pKa = 4.04 DD1507 pKa = 3.68 QKK1509 pKa = 12.0 DD1510 pKa = 3.4 KK1511 pKa = 10.57 LTSNLEE1517 pKa = 4.04 RR1518 pKa = 11.84 LAGALLFGGYY1528 pKa = 8.81 RR1529 pKa = 11.84 RR1530 pKa = 11.84 EE1531 pKa = 4.23 ARR1533 pKa = 11.84 WMLYY1537 pKa = 8.58 NAKK1540 pKa = 10.41 DD1541 pKa = 3.25 WMKK1544 pKa = 10.45 EE1545 pKa = 3.65 QLGYY1549 pKa = 10.75 FPDD1552 pKa = 4.55 DD1553 pKa = 3.81 PSWINLRR1560 pKa = 11.84 VMAMLANAKK1569 pKa = 9.77 GIKK1572 pKa = 9.09 LWSINSSQRR1581 pKa = 11.84 QITEE1585 pKa = 3.66 WAANTAQNVIPATVYY1600 pKa = 10.42 LARR1603 pKa = 11.84 DD1604 pKa = 3.34 GLTIYY1609 pKa = 9.38 PCSMGTNAAKK1619 pKa = 9.03 LTSILRR1625 pKa = 11.84 FNAHH1629 pKa = 6.49 CKK1631 pKa = 9.62 RR1632 pKa = 11.84 LKK1634 pKa = 10.41 DD1635 pKa = 3.52 EE1636 pKa = 5.17 HH1637 pKa = 8.21 GSIPEE1642 pKa = 4.41 PPSEE1646 pKa = 5.1 LPTIPDD1652 pKa = 3.33 ARR1654 pKa = 11.84 VKK1656 pKa = 9.65 TALAVGSVAAIALGGIAVGAIIAVSVWTVYY1686 pKa = 10.32 RR1687 pKa = 11.84 KK1688 pKa = 9.38 RR1689 pKa = 11.84 VKK1691 pKa = 10.5 AGSIGAAVNEE1701 pKa = 4.28 LVRR1704 pKa = 11.84 GQNTATDD1711 pKa = 3.85 AEE1713 pKa = 4.72 FEE1715 pKa = 4.33 QPLLAVEE1722 pKa = 4.83 EE1723 pKa = 4.31 KK1724 pKa = 10.88 SVWDD1728 pKa = 4.73 DD1729 pKa = 3.04 GWSLSSANHH1738 pKa = 7.02 DD1739 pKa = 3.95 EE1740 pKa = 4.6 GEE1742 pKa = 4.3 VMPDD1746 pKa = 3.0 RR1747 pKa = 11.84 LSQYY1751 pKa = 10.15 TSMAVPLGGDD1761 pKa = 3.55 PLGVTPAGPDD1771 pKa = 2.75 NAAPVRR1777 pKa = 11.84 SWTRR1781 pKa = 11.84 AMLSKK1786 pKa = 10.52 MNN1788 pKa = 4.03
Molecular weight: 197.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.33
IPC2_protein 6.338
IPC_protein 6.415
Toseland 6.707
ProMoST 6.825
Dawson 6.766
Bjellqvist 6.766
Wikipedia 6.781
Rodwell 6.766
Grimsley 6.868
Solomon 6.766
Lehninger 6.766
Nozaki 7.044
DTASelect 7.205
Thurlkill 7.234
EMBOSS 7.205
Sillero 7.146
Patrickios 4.317
IPC_peptide 6.781
IPC2_peptide 6.868
IPC2.peptide.svr19 6.87
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
3690
1788
1902
1845.0
205.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.244 ± 0.866
1.192 ± 0.221
5.149 ± 0.039
6.992 ± 0.458
2.304 ± 0.158
6.396 ± 0.068
2.168 ± 0.093
4.472 ± 0.334
5.908 ± 0.098
7.995 ± 0.122
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.333 ± 0.1
3.794 ± 0.035
4.526 ± 0.08
3.686 ± 0.245
5.691 ± 0.364
6.694 ± 0.055
6.341 ± 0.192
7.344 ± 0.262
1.816 ± 0.144
3.93 ± 0.094
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here