Methylocella tundrae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Beijerinckiaceae; Methylocella

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4853 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V6IN84|A0A4V6IN84_METTU Pyruvate dehydrogenase E1 component subunit alpha OS=Methylocella tundrae OX=227605 GN=pdhA PE=4 SV=1
MM1 pKa = 7.85ASNDD5 pKa = 2.63IDD7 pKa = 3.84VFVICEE13 pKa = 3.76AAAIAPGAAKK23 pKa = 10.3AFSLSRR29 pKa = 11.84INDD32 pKa = 3.51AGDD35 pKa = 3.83PEE37 pKa = 4.5TFPIFVIHH45 pKa = 6.12TAADD49 pKa = 3.78EE50 pKa = 4.05YY51 pKa = 11.15LGYY54 pKa = 11.38VNACPHH60 pKa = 6.6KK61 pKa = 10.69GVLLNKK67 pKa = 10.41GPGTFFTQDD76 pKa = 3.05RR77 pKa = 11.84KK78 pKa = 10.7LLEE81 pKa = 4.75CGQHH85 pKa = 5.97GAMFDD90 pKa = 3.59IDD92 pKa = 3.55TGLCVDD98 pKa = 5.3GPCKK102 pKa = 10.3AQSLEE107 pKa = 4.12PVALAVIDD115 pKa = 5.48GDD117 pKa = 3.66ICLCGVKK124 pKa = 10.18LVEE127 pKa = 5.96DD128 pKa = 4.68DD129 pKa = 5.21GIPNPFEE136 pKa = 4.26MPDD139 pKa = 3.83DD140 pKa = 4.6GPVVLIDD147 pKa = 3.71SDD149 pKa = 3.81

Molecular weight: 15.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4U8Z3T4|A0A4U8Z3T4_METTU Acyl-CoA thioesterase II OS=Methylocella tundrae OX=227605 GN=tesB PE=3 SV=1
MM1 pKa = 7.62WFTRR5 pKa = 11.84PPHH8 pKa = 6.56ALTAAARR15 pKa = 11.84GIPTRR20 pKa = 11.84AQAKK24 pKa = 9.06AKK26 pKa = 10.25ALNRR30 pKa = 11.84HH31 pKa = 5.65FWRR34 pKa = 11.84AFAQSARR41 pKa = 11.84RR42 pKa = 11.84TLIASSIPRR51 pKa = 11.84RR52 pKa = 11.84QSRR55 pKa = 11.84RR56 pKa = 11.84SS57 pKa = 3.25

Molecular weight: 6.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4207

646

4853

1304048

20

2702

268.7

29.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.189 ± 0.055

0.943 ± 0.01

5.499 ± 0.026

5.555 ± 0.036

3.914 ± 0.025

8.298 ± 0.033

2.055 ± 0.015

5.284 ± 0.027

3.514 ± 0.028

10.094 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.184 ± 0.015

2.586 ± 0.023

5.428 ± 0.028

3.069 ± 0.021

7.631 ± 0.044

5.848 ± 0.024

4.753 ± 0.025

6.777 ± 0.029

1.27 ± 0.015

2.107 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski