Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Rhodobacter; Rhodobacter capsulatus

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3632 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D5AMD5|D5AMD5_RHOCB zf-CHCC domain-containing protein OS=Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003) OX=272942 GN=RCAP_rcc02481 PE=4 SV=1
MM1 pKa = 8.23PITGLPVTSGNNTVTVTPTGIYY23 pKa = 9.84SVNAEE28 pKa = 4.24GGVDD32 pKa = 4.01TLVMDD37 pKa = 4.27YY38 pKa = 10.13STLTSDD44 pKa = 5.23IIYY47 pKa = 9.69EE48 pKa = 4.01YY49 pKa = 10.73AGNGFYY55 pKa = 10.85RR56 pKa = 11.84FTDD59 pKa = 3.81YY60 pKa = 11.1LSSSIDD66 pKa = 3.5FYY68 pKa = 11.49NFEE71 pKa = 4.25RR72 pKa = 11.84FILRR76 pKa = 11.84GGSGDD81 pKa = 4.0DD82 pKa = 4.24DD83 pKa = 3.94LRR85 pKa = 11.84GANDD89 pKa = 2.93VDD91 pKa = 3.68QLYY94 pKa = 10.78GGAGADD100 pKa = 3.68SLTSGLGADD109 pKa = 4.11VVNGGAGFDD118 pKa = 3.37RR119 pKa = 11.84WTAGYY124 pKa = 8.05GTVTGNVLITLSADD138 pKa = 3.32PAITQLVAATGARR151 pKa = 11.84FTQIEE156 pKa = 4.35ALSITTGIGDD166 pKa = 4.0DD167 pKa = 4.48VINTSAFVGNDD178 pKa = 3.94DD179 pKa = 3.57IATGNGSDD187 pKa = 3.47QVNAGRR193 pKa = 11.84GVDD196 pKa = 3.99WINGGEE202 pKa = 4.33GTDD205 pKa = 2.95IMVFDD210 pKa = 4.72WSAITDD216 pKa = 3.58PSFAIRR222 pKa = 11.84NSYY225 pKa = 8.95VSNGWYY231 pKa = 9.89RR232 pKa = 11.84YY233 pKa = 9.21AALSGDD239 pKa = 3.3QVNYY243 pKa = 10.47INFEE247 pKa = 4.27RR248 pKa = 11.84YY249 pKa = 9.13FLTGGAGNDD258 pKa = 3.51WLSGAGQADD267 pKa = 4.37RR268 pKa = 11.84LTGNGGNDD276 pKa = 3.46TLIGGTGADD285 pKa = 4.31TIAGGAGIDD294 pKa = 3.58LWQVDD299 pKa = 4.3LTDD302 pKa = 3.79IEE304 pKa = 5.33ANTSVSLVSQTSNTGAVLSGIEE326 pKa = 3.95RR327 pKa = 11.84LTYY330 pKa = 9.79TGSAFDD336 pKa = 4.14DD337 pKa = 3.77TVVAQSGSYY346 pKa = 10.4NDD348 pKa = 4.08SFTMGAGDD356 pKa = 4.14DD357 pKa = 3.68AVTTGRR363 pKa = 11.84GVDD366 pKa = 3.75GANGEE371 pKa = 4.22AGEE374 pKa = 5.0DD375 pKa = 3.45LLVMNWSGITDD386 pKa = 3.85ATQGISRR393 pKa = 11.84SYY395 pKa = 10.92VSNGWYY401 pKa = 9.94RR402 pKa = 11.84FSAASGDD409 pKa = 3.53RR410 pKa = 11.84LDD412 pKa = 4.38YY413 pKa = 11.3INFEE417 pKa = 4.31RR418 pKa = 11.84FFLTGGAGNDD428 pKa = 3.81YY429 pKa = 11.17LLGAALNDD437 pKa = 3.5RR438 pKa = 11.84LVGNGGDD445 pKa = 3.81DD446 pKa = 3.67TLSGGGGVDD455 pKa = 4.85AITGGDD461 pKa = 4.43GVDD464 pKa = 2.93LWEE467 pKa = 5.09ANLSEE472 pKa = 5.07HH473 pKa = 5.66EE474 pKa = 4.47TNVTINFQTQTTNYY488 pKa = 8.56GAVISGLEE496 pKa = 3.86RR497 pKa = 11.84LNYY500 pKa = 9.57TGTAVRR506 pKa = 11.84DD507 pKa = 4.24LITTRR512 pKa = 11.84SGAYY516 pKa = 9.85DD517 pKa = 3.61DD518 pKa = 4.81NLYY521 pKa = 10.89LGAGNDD527 pKa = 3.54SAAVWRR533 pKa = 11.84GVDD536 pKa = 3.33AVNGAEE542 pKa = 5.07GEE544 pKa = 4.27DD545 pKa = 3.82TLILDD550 pKa = 4.01WSGIAGATQGITYY563 pKa = 10.01SYY565 pKa = 10.85VSNGWYY571 pKa = 10.01RR572 pKa = 11.84FSSTSGDD579 pKa = 3.23QVDD582 pKa = 5.49FINIEE587 pKa = 4.2HH588 pKa = 6.87YY589 pKa = 11.51DD590 pKa = 3.5MTGGAGHH597 pKa = 7.56DD598 pKa = 3.86SLVGGTMNDD607 pKa = 3.59TLRR610 pKa = 11.84GGAGNDD616 pKa = 3.7TLDD619 pKa = 3.7SGAGRR624 pKa = 11.84AVIDD628 pKa = 4.03GGAGTDD634 pKa = 3.16LWRR637 pKa = 11.84ADD639 pKa = 3.91LSAISAGLRR648 pKa = 11.84FSASASQTAAQATGSGCDD666 pKa = 3.23VRR668 pKa = 11.84NIEE671 pKa = 4.3QVSLTGGAGADD682 pKa = 4.32LIDD685 pKa = 3.67TTGQSGNDD693 pKa = 3.2WFAGNAGNDD702 pKa = 3.81TVSLGLGQDD711 pKa = 3.74GFDD714 pKa = 3.7GGAGVDD720 pKa = 4.96LLILDD725 pKa = 4.4YY726 pKa = 11.01SASTEE731 pKa = 4.45AIQSRR736 pKa = 11.84YY737 pKa = 7.43TSNGWNRR744 pKa = 11.84YY745 pKa = 8.07GEE747 pKa = 4.02QGDD750 pKa = 3.87AHH752 pKa = 6.66FLDD755 pKa = 4.81YY756 pKa = 11.33INVEE760 pKa = 4.17RR761 pKa = 11.84FNVTGGAGADD771 pKa = 3.57TLIGAGAADD780 pKa = 4.52TLTGGAGNDD789 pKa = 3.6WLNGGAGADD798 pKa = 4.11VINGGAGVDD807 pKa = 3.19TWQASFAGATVAIGLTLSAAGAATVSGAGTVLTGIEE843 pKa = 4.3AVSLTTGQNADD854 pKa = 3.6IVNLSAGTGNDD865 pKa = 2.79AVATNEE871 pKa = 4.16GNDD874 pKa = 3.65TVNLGRR880 pKa = 11.84GRR882 pKa = 11.84SEE884 pKa = 3.87WADD887 pKa = 3.16GGAGTDD893 pKa = 3.54SLTLNASLATSGLRR907 pKa = 11.84MDD909 pKa = 4.15YY910 pKa = 10.77VSNGAYY916 pKa = 10.01HH917 pKa = 7.57IYY919 pKa = 9.57ATDD922 pKa = 3.59GSYY925 pKa = 11.38DD926 pKa = 3.17ATFNNFEE933 pKa = 4.53TYY935 pKa = 10.62NITGSARR942 pKa = 11.84NDD944 pKa = 2.98RR945 pKa = 11.84LYY947 pKa = 11.31AFGGNDD953 pKa = 4.19RR954 pKa = 11.84ISTGAGNDD962 pKa = 3.23ILNGGDD968 pKa = 4.25GNDD971 pKa = 3.8TLLGGAGADD980 pKa = 3.07VFQFTNLYY988 pKa = 9.91GCGIDD993 pKa = 4.18TIADD997 pKa = 3.66GAIGDD1002 pKa = 4.09RR1003 pKa = 11.84LRR1005 pKa = 11.84LSGLALSSIANGAGTTLGAGQIAVSASGGVTTLAIGLDD1043 pKa = 3.83GTAGADD1049 pKa = 3.22LTIRR1053 pKa = 11.84LTGSFTAADD1062 pKa = 4.51FSLSGSDD1069 pKa = 3.92VIFII1073 pKa = 4.75

Molecular weight: 109.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D5AM50|D5AM50_RHOCB S1 RNA binding domain protein OS=Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003) OX=272942 GN=RCAP_rcc00355 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.43GGRR28 pKa = 11.84KK29 pKa = 8.99VLNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.97VLSAA44 pKa = 4.11

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3632

0

3632

1160234

28

2145

319.4

34.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.118 ± 0.079

0.878 ± 0.013

5.491 ± 0.037

5.738 ± 0.044

3.567 ± 0.025

8.913 ± 0.047

1.922 ± 0.018

4.742 ± 0.03

2.983 ± 0.037

10.712 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.558 ± 0.022

2.12 ± 0.025

5.553 ± 0.041

3.025 ± 0.024

7.241 ± 0.044

4.648 ± 0.029

5.39 ± 0.037

7.102 ± 0.035

1.367 ± 0.017

1.932 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski