Lentilactobacillus curieae
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1930 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1S6QJE4|A0A1S6QJE4_9LACO Ribonuclease M5 OS=Lentilactobacillus curieae OX=1138822 GN=rnmV PE=3 SV=1
MM1 pKa = 7.53 INLASAHH8 pKa = 5.1 VVYY11 pKa = 9.01 ATITGNNEE19 pKa = 3.5 DD20 pKa = 3.54 VADD23 pKa = 5.08 IITEE27 pKa = 3.94 HH28 pKa = 7.98 LEE30 pKa = 3.93 DD31 pKa = 4.38 LGIDD35 pKa = 3.56 VSEE38 pKa = 4.5 TEE40 pKa = 4.57 ISQTDD45 pKa = 3.37 PSVFEE50 pKa = 5.27 DD51 pKa = 3.25 VDD53 pKa = 3.46 ICIVCPYY60 pKa = 9.68 TYY62 pKa = 10.69 DD63 pKa = 3.35 EE64 pKa = 4.73 GALPEE69 pKa = 4.34 EE70 pKa = 4.73 GMDD73 pKa = 3.92 FYY75 pKa = 11.69 EE76 pKa = 5.79 DD77 pKa = 4.92 LPEE80 pKa = 5.83 LDD82 pKa = 5.18 LNGKK86 pKa = 9.27 VYY88 pKa = 10.53 GVAGSGDD95 pKa = 3.3 TFYY98 pKa = 11.69 EE99 pKa = 3.94 EE100 pKa = 4.93 FYY102 pKa = 11.4 NLAVDD107 pKa = 3.9 KK108 pKa = 10.89 FDD110 pKa = 4.15 AAFAKK115 pKa = 10.04 TGATKK120 pKa = 10.34 GAEE123 pKa = 3.99 SVKK126 pKa = 10.52 INLEE130 pKa = 4.04 PGEE133 pKa = 4.18 EE134 pKa = 4.51 DD135 pKa = 3.86 IANLDD140 pKa = 3.71 KK141 pKa = 10.8 FVEE144 pKa = 4.96 AIVEE148 pKa = 4.07 KK149 pKa = 10.97 SKK151 pKa = 11.43
Molecular weight: 16.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.753
IPC2_protein 3.77
IPC_protein 3.732
Toseland 3.541
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.617
Rodwell 3.567
Grimsley 3.452
Solomon 3.706
Lehninger 3.656
Nozaki 3.834
DTASelect 4.012
Thurlkill 3.579
EMBOSS 3.63
Sillero 3.859
Patrickios 1.837
IPC_peptide 3.706
IPC2_peptide 3.834
IPC2.peptide.svr19 3.779
Protein with the highest isoelectric point:
>tr|A0A1S6QHU9|A0A1S6QHU9_9LACO Phosphate transport system permease protein OS=Lentilactobacillus curieae OX=1138822 GN=PL11_004215 PE=3 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.69 QPKK8 pKa = 8.19 KK9 pKa = 7.37 RR10 pKa = 11.84 HH11 pKa = 5.5 RR12 pKa = 11.84 ARR14 pKa = 11.84 VHH16 pKa = 5.84 GFRR19 pKa = 11.84 KK20 pKa = 10.03 RR21 pKa = 11.84 MSTSNGRR28 pKa = 11.84 KK29 pKa = 8.01 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 10.55 GRR39 pKa = 11.84 KK40 pKa = 8.96 SLTAA44 pKa = 4.07
Molecular weight: 5.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.398
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.135
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.06
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1930
0
1930
602619
39
3597
312.2
34.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.65 ± 0.153
0.401 ± 0.012
6.076 ± 0.061
5.615 ± 0.073
4.46 ± 0.046
6.898 ± 0.054
1.921 ± 0.021
7.318 ± 0.061
6.904 ± 0.057
9.058 ± 0.075
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.678 ± 0.032
5.291 ± 0.044
3.553 ± 0.029
3.925 ± 0.036
3.63 ± 0.042
6.755 ± 0.084
5.976 ± 0.041
7.426 ± 0.045
0.962 ± 0.021
3.502 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here