Hypoxylon sp. CO27-5

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Xylariomycetidae; Xylariales; Hypoxylaceae; Hypoxylon; unclassified Hypoxylon

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12244 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1Y2V5S5|A0A1Y2V5S5_9PEZI MFS domain-containing protein OS=Hypoxylon sp. CO27-5 OX=1001938 GN=M434DRAFT_75144 PE=4 SV=1
MM1 pKa = 7.5SYY3 pKa = 10.43AGPMHH8 pKa = 6.47PHH10 pKa = 6.58ALVLEE15 pKa = 4.63DD16 pKa = 3.83FHH18 pKa = 9.51AEE20 pKa = 3.7VSTEE24 pKa = 3.87LTVKK28 pKa = 10.46KK29 pKa = 10.33GEE31 pKa = 4.17HH32 pKa = 5.99VFLIWTHH39 pKa = 5.29EE40 pKa = 4.4DD41 pKa = 2.52GWYY44 pKa = 9.77VCNSKK49 pKa = 10.7NVFGCVPNRR58 pKa = 11.84FLKK61 pKa = 10.44PDD63 pKa = 3.48GYY65 pKa = 11.22GQLKK69 pKa = 9.24TDD71 pKa = 3.44VPPIKK76 pKa = 10.61FPDD79 pKa = 3.64TDD81 pKa = 4.22DD82 pKa = 3.99EE83 pKa = 4.72NGSEE87 pKa = 4.3TEE89 pKa = 3.93SDD91 pKa = 3.63ADD93 pKa = 3.94SSDD96 pKa = 4.63DD97 pKa = 4.6SGDD100 pKa = 4.13DD101 pKa = 3.74NSDD104 pKa = 3.56TCSCSSDD111 pKa = 3.3EE112 pKa = 4.74DD113 pKa = 4.44DD114 pKa = 3.75EE115 pKa = 5.34EE116 pKa = 4.69EE117 pKa = 4.7YY118 pKa = 10.96EE119 pKa = 4.38DD120 pKa = 4.19EE121 pKa = 6.16AVASWW126 pKa = 3.08

Molecular weight:
14.03 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1Y2VA98|A0A1Y2VA98_9PEZI Pre-rRNA-processing protein PNO1 OS=Hypoxylon sp. CO27-5 OX=1001938 GN=M434DRAFT_395426 PE=3 SV=1
MM1 pKa = 7.88PSHH4 pKa = 6.91KK5 pKa = 10.39SFRR8 pKa = 11.84TKK10 pKa = 10.45QKK12 pKa = 9.84LAKK15 pKa = 9.55AQKK18 pKa = 8.59QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.14WRR45 pKa = 11.84KK46 pKa = 7.41TRR48 pKa = 11.84LGII51 pKa = 4.46

Molecular weight:
6.25 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12244

0

12244

5616406

50

4917

458.7

50.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.289 ± 0.02

1.251 ± 0.009

5.724 ± 0.016

6.165 ± 0.024

3.794 ± 0.015

6.851 ± 0.021

2.337 ± 0.01

5.243 ± 0.015

4.929 ± 0.022

8.888 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.108 ± 0.008

3.86 ± 0.013

6.016 ± 0.023

3.856 ± 0.015

6.087 ± 0.018

8.123 ± 0.027

5.957 ± 0.012

6.082 ± 0.015

1.528 ± 0.008

2.912 ± 0.011

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski