Hypoxylon sp. CO27-5
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12244 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y2V5S5|A0A1Y2V5S5_9PEZI MFS domain-containing protein OS=Hypoxylon sp. CO27-5 OX=1001938 GN=M434DRAFT_75144 PE=4 SV=1
MM1 pKa = 7.5 SYY3 pKa = 10.43 AGPMHH8 pKa = 6.47 PHH10 pKa = 6.58 ALVLEE15 pKa = 4.63 DD16 pKa = 3.83 FHH18 pKa = 9.51 AEE20 pKa = 3.7 VSTEE24 pKa = 3.87 LTVKK28 pKa = 10.46 KK29 pKa = 10.33 GEE31 pKa = 4.17 HH32 pKa = 5.99 VFLIWTHH39 pKa = 5.29 EE40 pKa = 4.4 DD41 pKa = 2.52 GWYY44 pKa = 9.77 VCNSKK49 pKa = 10.7 NVFGCVPNRR58 pKa = 11.84 FLKK61 pKa = 10.44 PDD63 pKa = 3.48 GYY65 pKa = 11.22 GQLKK69 pKa = 9.24 TDD71 pKa = 3.44 VPPIKK76 pKa = 10.61 FPDD79 pKa = 3.64 TDD81 pKa = 4.22 DD82 pKa = 3.99 EE83 pKa = 4.72 NGSEE87 pKa = 4.3 TEE89 pKa = 3.93 SDD91 pKa = 3.63 ADD93 pKa = 3.94 SSDD96 pKa = 4.63 DD97 pKa = 4.6 SGDD100 pKa = 4.13 DD101 pKa = 3.74 NSDD104 pKa = 3.56 TCSCSSDD111 pKa = 3.3 EE112 pKa = 4.74 DD113 pKa = 4.44 DD114 pKa = 3.75 EE115 pKa = 5.34 EE116 pKa = 4.69 EE117 pKa = 4.7 YY118 pKa = 10.96 EE119 pKa = 4.38 DD120 pKa = 4.19 EE121 pKa = 6.16 AVASWW126 pKa = 3.08
Molecular weight: 14.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.748
IPC2_protein 3.872
IPC_protein 3.859
Toseland 3.656
ProMoST 4.024
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.77
Rodwell 3.694
Grimsley 3.567
Solomon 3.846
Lehninger 3.795
Nozaki 3.961
DTASelect 4.177
Thurlkill 3.706
EMBOSS 3.783
Sillero 3.986
Patrickios 1.901
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.869
Protein with the highest isoelectric point:
>tr|A0A1Y2VA98|A0A1Y2VA98_9PEZI Pre-rRNA-processing protein PNO1 OS=Hypoxylon sp. CO27-5 OX=1001938 GN=M434DRAFT_395426 PE=3 SV=1
MM1 pKa = 7.88 PSHH4 pKa = 6.91 KK5 pKa = 10.39 SFRR8 pKa = 11.84 TKK10 pKa = 10.45 QKK12 pKa = 9.84 LAKK15 pKa = 9.55 AQKK18 pKa = 8.59 QNRR21 pKa = 11.84 PIPQWIRR28 pKa = 11.84 LRR30 pKa = 11.84 TGNTIRR36 pKa = 11.84 YY37 pKa = 5.79 NAKK40 pKa = 8.89 RR41 pKa = 11.84 RR42 pKa = 11.84 HH43 pKa = 4.14 WRR45 pKa = 11.84 KK46 pKa = 7.41 TRR48 pKa = 11.84 LGII51 pKa = 4.46
Molecular weight: 6.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.39
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.34
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.076
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.006
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12244
0
12244
5616406
50
4917
458.7
50.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.289 ± 0.02
1.251 ± 0.009
5.724 ± 0.016
6.165 ± 0.024
3.794 ± 0.015
6.851 ± 0.021
2.337 ± 0.01
5.243 ± 0.015
4.929 ± 0.022
8.888 ± 0.022
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.108 ± 0.008
3.86 ± 0.013
6.016 ± 0.023
3.856 ± 0.015
6.087 ± 0.018
8.123 ± 0.027
5.957 ± 0.012
6.082 ± 0.015
1.528 ± 0.008
2.912 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here