Giant panda circovirus 3
Average proteome isoelectric point is 7.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A220IGT2|A0A220IGT2_9CIRC Capsid protein OS=Giant panda circovirus 3 OX=2016458 PE=4 SV=1
MM1 pKa = 6.66 TQEE4 pKa = 3.59 ITQISDD10 pKa = 3.37 ANDD13 pKa = 3.1 VSDD16 pKa = 3.77 SFEE19 pKa = 4.03 TKK21 pKa = 10.68 FIVKK25 pKa = 9.8 NSKK28 pKa = 10.03 QVTTVINTSNASVLCQSYY46 pKa = 8.63 FCRR49 pKa = 11.84 VRR51 pKa = 11.84 YY52 pKa = 9.89 DD53 pKa = 2.77 IGTGQNYY60 pKa = 9.58 QDD62 pKa = 3.92 LCQLLGDD69 pKa = 4.42 GFFQRR74 pKa = 11.84 GLDD77 pKa = 3.84 TQSGSANSGVSDD89 pKa = 4.44 ASLTPFDD96 pKa = 3.52 SHH98 pKa = 6.98 KK99 pKa = 10.14 FCSYY103 pKa = 10.67 FNIFKK108 pKa = 8.76 VQKK111 pKa = 10.02 YY112 pKa = 7.41 EE113 pKa = 4.15 LEE115 pKa = 4.5 AGATKK120 pKa = 10.15 MLTVQTNKK128 pKa = 10.08 PYY130 pKa = 10.25 TVNTSHH136 pKa = 7.4 LFTGSSRR143 pKa = 11.84 TFPPTFSPCILHH155 pKa = 6.84 KK156 pKa = 10.23 KK157 pKa = 10.37 GEE159 pKa = 4.26 MFVFTKK165 pKa = 10.66 FEE167 pKa = 4.72 GIPADD172 pKa = 3.76 SQSGQWYY179 pKa = 5.71 TTPKK183 pKa = 10.35 LDD185 pKa = 4.41 TITKK189 pKa = 6.75 THH191 pKa = 6.15 YY192 pKa = 10.28 NYY194 pKa = 10.69 VMLSPNPPTLLRR206 pKa = 11.84 KK207 pKa = 8.94 PATGYY212 pKa = 7.07 TAPGANALTIMQEE225 pKa = 3.82 EE226 pKa = 4.59 SGNNVPQTNAA236 pKa = 3.22
Molecular weight: 26.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.306
IPC2_protein 6.364
IPC_protein 6.561
Toseland 6.532
ProMoST 6.985
Dawson 7.059
Bjellqvist 6.883
Wikipedia 7.029
Rodwell 7.044
Grimsley 6.605
Solomon 7.102
Lehninger 7.117
Nozaki 7.234
DTASelect 7.322
Thurlkill 7.366
EMBOSS 7.395
Sillero 7.454
Patrickios 3.719
IPC_peptide 7.102
IPC2_peptide 6.956
IPC2.peptide.svr19 6.932
Protein with the highest isoelectric point:
>tr|A0A220IGT2|A0A220IGT2_9CIRC Capsid protein OS=Giant panda circovirus 3 OX=2016458 PE=4 SV=1
MM1 pKa = 7.51 TNFFFVIFHH10 pKa = 6.8 LLITFPNMTPRR21 pKa = 11.84 NISDD25 pKa = 3.2 YY26 pKa = 9.06 HH27 pKa = 6.45 CAVSFCTQPYY37 pKa = 8.64 QDD39 pKa = 4.63 SGYY42 pKa = 8.9 MCYY45 pKa = 10.41 FSAAPNARR53 pKa = 11.84 RR54 pKa = 11.84 AYY56 pKa = 9.89 RR57 pKa = 11.84 SLPSRR62 pKa = 11.84 SNMVARR68 pKa = 11.84 KK69 pKa = 9.39 SFLRR73 pKa = 11.84 ARR75 pKa = 11.84 ASKK78 pKa = 9.68 RR79 pKa = 11.84 RR80 pKa = 11.84 PRR82 pKa = 11.84 RR83 pKa = 11.84 RR84 pKa = 11.84 VVKK87 pKa = 10.24 RR88 pKa = 11.84 KK89 pKa = 9.41 RR90 pKa = 11.84 VARR93 pKa = 11.84 KK94 pKa = 7.97 PRR96 pKa = 11.84 RR97 pKa = 11.84 RR98 pKa = 11.84 TRR100 pKa = 11.84 KK101 pKa = 6.86 TPKK104 pKa = 10.39 NGVQWKK110 pKa = 7.96 PANILSLDD118 pKa = 3.63 YY119 pKa = 11.54 DD120 pKa = 3.82 NADD123 pKa = 3.36 TRR125 pKa = 11.84 LVTAGSAIQGKK136 pKa = 9.23 RR137 pKa = 11.84 CPP139 pKa = 3.8
Molecular weight: 16.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.173
IPC2_protein 10.321
IPC_protein 11.52
Toseland 11.637
ProMoST 12.076
Dawson 11.652
Bjellqvist 11.608
Wikipedia 12.091
Rodwell 11.491
Grimsley 11.696
Solomon 12.106
Lehninger 12.003
Nozaki 11.637
DTASelect 11.608
Thurlkill 11.637
EMBOSS 12.12
Sillero 11.637
Patrickios 11.199
IPC_peptide 12.106
IPC2_peptide 11.082
IPC2.peptide.svr19 9.13
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
674
139
299
224.7
25.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.38 ± 0.988
2.819 ± 0.3
4.748 ± 0.4
4.303 ± 1.613
6.231 ± 0.153
6.231 ± 1.112
2.077 ± 0.309
3.858 ± 0.162
6.083 ± 0.128
5.341 ± 0.423
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.374 ± 0.172
5.786 ± 0.246
4.451 ± 1.15
4.599 ± 0.808
7.27 ± 3.067
8.012 ± 0.563
8.012 ± 1.621
5.045 ± 0.357
1.78 ± 0.778
4.599 ± 0.208
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here