Chimpanzee faeces associated microphage 3

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A186YBY4|A0A186YBY4_9VIRU Minor CP 1 OS=Chimpanzee faeces associated microphage 3 OX=1676183 PE=4 SV=1
MM1 pKa = 7.63TFFTLFNKK9 pKa = 9.8PSPVGTACNTPSLTIQSEE27 pKa = 4.28KK28 pKa = 10.56AQCDD32 pKa = 3.02INAIIARR39 pKa = 11.84YY40 pKa = 9.21KK41 pKa = 8.51KK42 pKa = 9.09TGVVDD47 pKa = 4.5HH48 pKa = 7.23IKK50 pKa = 10.23RR51 pKa = 11.84DD52 pKa = 3.47QPLYY56 pKa = 10.8ADD58 pKa = 4.54CEE60 pKa = 4.07QAITDD65 pKa = 4.08LEE67 pKa = 4.16KK68 pKa = 11.12ARR70 pKa = 11.84ILVEE74 pKa = 4.04DD75 pKa = 3.98TEE77 pKa = 4.52EE78 pKa = 4.29AFWMLPSSVRR88 pKa = 11.84DD89 pKa = 3.78LIGEE93 pKa = 4.27PSNLPTWASVNRR105 pKa = 11.84SEE107 pKa = 4.26AEE109 pKa = 3.68KK110 pKa = 11.08YY111 pKa = 10.98GFIKK115 pKa = 10.02PQSTPPVDD123 pKa = 3.71SATPPPVDD131 pKa = 3.46SAANGG136 pKa = 3.39

Molecular weight: 14.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A186YBY4|A0A186YBY4_9VIRU Minor CP 1 OS=Chimpanzee faeces associated microphage 3 OX=1676183 PE=4 SV=1
MM1 pKa = 7.62ARR3 pKa = 11.84PPPNWRR9 pKa = 11.84RR10 pKa = 11.84LFCLKK15 pKa = 10.16GDD17 pKa = 3.71YY18 pKa = 9.54MAFGVDD24 pKa = 3.48DD25 pKa = 4.43ALFAAVAGGSQGASNIAGAFLGDD48 pKa = 3.62YY49 pKa = 9.87FNRR52 pKa = 11.84KK53 pKa = 8.51AASKK57 pKa = 9.92QRR59 pKa = 11.84QASWDD64 pKa = 3.57MLLAEE69 pKa = 4.24QAFNARR75 pKa = 11.84EE76 pKa = 3.98AQKK79 pKa = 10.6NRR81 pKa = 11.84EE82 pKa = 3.94WQEE85 pKa = 3.82RR86 pKa = 11.84LSNTAHH92 pKa = 5.37QRR94 pKa = 11.84EE95 pKa = 4.52VADD98 pKa = 4.56LRR100 pKa = 11.84AAGLNPVLSATGGNGASTGSGAMATSNMSAPDD132 pKa = 4.02LSALAQSGQAFSRR145 pKa = 11.84VGNGVVDD152 pKa = 3.76KK153 pKa = 10.85ALRR156 pKa = 11.84AASLRR161 pKa = 11.84SDD163 pKa = 3.44IKK165 pKa = 11.28LMEE168 pKa = 4.86SNASNAEE175 pKa = 3.87AQAANSWEE183 pKa = 3.87NVLYY187 pKa = 10.44TKK189 pKa = 11.16ANTAKK194 pKa = 9.96VLAEE198 pKa = 4.0AGVFEE203 pKa = 4.38SQLDD207 pKa = 3.8RR208 pKa = 11.84IEE210 pKa = 3.94WLKK213 pKa = 11.14RR214 pKa = 11.84NSPNTWYY221 pKa = 10.64LWGDD225 pKa = 3.34KK226 pKa = 10.7DD227 pKa = 3.72PSVWQLLPGFSRR239 pKa = 11.84GSEE242 pKa = 3.94FGAQSVGNGVKK253 pKa = 9.88SVYY256 pKa = 10.77DD257 pKa = 3.61FVKK260 pKa = 10.73DD261 pKa = 4.53PIASAVKK268 pKa = 9.95KK269 pKa = 10.48VYY271 pKa = 10.6NAVKK275 pKa = 10.73DD276 pKa = 4.81FGANNASSSSNQSIGYY292 pKa = 9.29SSYY295 pKa = 11.85DD296 pKa = 3.31KK297 pKa = 11.15ASSSDD302 pKa = 3.58FGKK305 pKa = 10.86VPLTTTRR312 pKa = 11.84WRR314 pKa = 11.84KK315 pKa = 8.19GQPLRR320 pKa = 11.84SGKK323 pKa = 9.74FDD325 pKa = 3.5YY326 pKa = 11.27QEE328 pKa = 3.79VRR330 pKa = 11.84RR331 pKa = 11.84KK332 pKa = 10.04RR333 pKa = 11.84LEE335 pKa = 4.04SLKK338 pKa = 10.8KK339 pKa = 10.29

Molecular weight: 36.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1315

136

558

328.8

36.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.365 ± 2.031

1.597 ± 0.545

6.54 ± 0.42

4.411 ± 0.407

5.095 ± 0.427

7.072 ± 0.538

1.749 ± 0.594

3.27 ± 0.762

5.019 ± 0.606

8.213 ± 0.687

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.825 ± 0.078

5.171 ± 0.56

5.399 ± 0.778

3.422 ± 0.605

6.236 ± 0.726

9.658 ± 0.605

4.867 ± 0.896

6.312 ± 0.731

2.205 ± 0.151

3.574 ± 0.417
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski