Peptostreptococcaceae bacterium pGA-8

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae; unclassified Peptostreptococcaceae

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1688 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I2ASK6|A0A1I2ASK6_9FIRM D-3-phosphoglycerate dehydrogenase OS=Peptostreptococcaceae bacterium pGA-8 OX=1520829 GN=SAMN02910327_01277 PE=3 SV=1
MM1 pKa = 7.51TDD3 pKa = 3.04KK4 pKa = 11.03EE5 pKa = 4.7KK6 pKa = 10.44YY7 pKa = 8.83TEE9 pKa = 3.96EE10 pKa = 4.55ADD12 pKa = 4.0IITLEE17 pKa = 3.86FDD19 pKa = 3.14EE20 pKa = 6.19GEE22 pKa = 4.42VEE24 pKa = 4.14CEE26 pKa = 3.24IMGIFEE32 pKa = 5.19HH33 pKa = 7.25GDD35 pKa = 3.07KK36 pKa = 10.64EE37 pKa = 4.49YY38 pKa = 10.71IALIPHH44 pKa = 7.66DD45 pKa = 4.65DD46 pKa = 3.58TDD48 pKa = 4.14DD49 pKa = 3.8VYY51 pKa = 11.24LYY53 pKa = 10.16EE54 pKa = 4.32YY55 pKa = 11.08KK56 pKa = 10.63EE57 pKa = 3.94VDD59 pKa = 3.19TDD61 pKa = 3.77DD62 pKa = 4.48EE63 pKa = 4.39EE64 pKa = 5.66VEE66 pKa = 4.45FEE68 pKa = 5.55LIDD71 pKa = 4.58IEE73 pKa = 5.28DD74 pKa = 4.34DD75 pKa = 3.94GLFEE79 pKa = 4.38EE80 pKa = 5.06VVKK83 pKa = 10.22TFEE86 pKa = 4.56EE87 pKa = 4.13IMAEE91 pKa = 4.0NEE93 pKa = 4.03EE94 pKa = 4.24NN95 pKa = 3.4

Molecular weight: 11.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I2ARW9|A0A1I2ARW9_9FIRM Uncharacterized protein OS=Peptostreptococcaceae bacterium pGA-8 OX=1520829 GN=SAMN02910327_01272 PE=4 SV=1
MM1 pKa = 7.49RR2 pKa = 11.84RR3 pKa = 11.84DD4 pKa = 3.38AFSGYY9 pKa = 9.89NPYY12 pKa = 11.09VNFIFYY18 pKa = 10.25IGVLGFSMILKK29 pKa = 10.06HH30 pKa = 6.57PIVLLTGFICAAAYY44 pKa = 9.82GGYY47 pKa = 9.99LRR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84ILRR55 pKa = 11.84TLFCFIFPMMLIGSLLNPLFNHH77 pKa = 7.21RR78 pKa = 11.84GQTILFYY85 pKa = 11.17LRR87 pKa = 11.84GNPMTLEE94 pKa = 3.91AMLFGVAAAFMVGTVILWFYY114 pKa = 9.37TFNDD118 pKa = 2.97VMTSDD123 pKa = 3.72KK124 pKa = 10.89LMHH127 pKa = 5.87VFGRR131 pKa = 11.84IIPGLNLLFSMVLRR145 pKa = 11.84FIPRR149 pKa = 11.84FNKK152 pKa = 10.14QIGVISNAQKK162 pKa = 10.82CIGRR166 pKa = 11.84DD167 pKa = 3.4VSNGSRR173 pKa = 11.84MDD175 pKa = 3.72RR176 pKa = 11.84ARR178 pKa = 11.84HH179 pKa = 4.14GMKK182 pKa = 9.67IISVMTTWGLEE193 pKa = 3.99NAVDD197 pKa = 4.9LGDD200 pKa = 3.81SMKK203 pKa = 10.83SRR205 pKa = 11.84GFGLRR210 pKa = 11.84GRR212 pKa = 11.84TSFFKK217 pKa = 11.0YY218 pKa = 10.25RR219 pKa = 11.84FDD221 pKa = 4.05SRR223 pKa = 11.84DD224 pKa = 3.1SRR226 pKa = 11.84LTLFMVTMAIIITYY240 pKa = 9.83GIVTGDD246 pKa = 3.7ANLEE250 pKa = 4.14YY251 pKa = 10.26YY252 pKa = 9.89PKK254 pKa = 10.61FIFPPFTLRR263 pKa = 11.84SIVTFIAYY271 pKa = 9.61FLFSSIPLAVDD282 pKa = 3.92FQEE285 pKa = 4.09DD286 pKa = 4.41LKK288 pKa = 10.84WKK290 pKa = 9.65RR291 pKa = 11.84LKK293 pKa = 10.89SRR295 pKa = 11.84II296 pKa = 3.79

Molecular weight: 34.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1688

0

1688

541392

39

2364

320.7

35.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.35 ± 0.061

1.185 ± 0.024

5.764 ± 0.045

7.516 ± 0.064

4.249 ± 0.043

7.35 ± 0.049

1.549 ± 0.025

8.443 ± 0.059

7.982 ± 0.055

8.788 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.954 ± 0.033

4.802 ± 0.037

3.164 ± 0.032

2.413 ± 0.028

4.086 ± 0.043

5.991 ± 0.043

5.111 ± 0.05

6.812 ± 0.044

0.772 ± 0.017

3.721 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski