Amino acid dipepetide frequency for Peptostreptococcaceae bacterium pGA-8

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.578AlaAla: 6.578 ± 0.141
0.9AlaCys: 0.9 ± 0.049
4.254AlaAsp: 4.254 ± 0.098
5.464AlaGlu: 5.464 ± 0.111
3.125AlaPhe: 3.125 ± 0.09
5.744AlaGly: 5.744 ± 0.122
1.058AlaHis: 1.058 ± 0.048
6.36AlaIle: 6.36 ± 0.126
5.371AlaLys: 5.371 ± 0.12
6.969AlaLeu: 6.969 ± 0.13
2.516AlaMet: 2.516 ± 0.073
2.867AlaAsn: 2.867 ± 0.077
1.919AlaPro: 1.919 ± 0.065
1.696AlaGln: 1.696 ± 0.062
2.708AlaArg: 2.708 ± 0.081
3.615AlaSer: 3.615 ± 0.087
3.517AlaThr: 3.517 ± 0.083
5.859AlaVal: 5.859 ± 0.123
0.447AlaTrp: 0.447 ± 0.029
2.532AlaTyr: 2.532 ± 0.072
0.0AlaXaa: 0.0 ± 0.0
Cys
0.779CysAla: 0.779 ± 0.04
0.185CysCys: 0.185 ± 0.022
0.715CysAsp: 0.715 ± 0.043
0.696CysGlu: 0.696 ± 0.032
0.473CysPhe: 0.473 ± 0.026
1.293CysGly: 1.293 ± 0.057
0.26CysHis: 0.26 ± 0.025
0.872CysIle: 0.872 ± 0.044
0.892CysLys: 0.892 ± 0.041
0.898CysLeu: 0.898 ± 0.041
0.279CysMet: 0.279 ± 0.025
0.573CysAsn: 0.573 ± 0.033
0.517CysPro: 0.517 ± 0.036
0.321CysGln: 0.321 ± 0.027
0.58CysArg: 0.58 ± 0.033
0.785CysSer: 0.785 ± 0.041
0.552CysThr: 0.552 ± 0.034
0.691CysVal: 0.691 ± 0.038
0.07CysTrp: 0.07 ± 0.011
0.412CysTyr: 0.412 ± 0.03
0.0CysXaa: 0.0 ± 0.0
Asp
3.873AspAla: 3.873 ± 0.091
0.611AspCys: 0.611 ± 0.034
3.127AspAsp: 3.127 ± 0.086
5.19AspGlu: 5.19 ± 0.103
2.861AspPhe: 2.861 ± 0.07
4.427AspGly: 4.427 ± 0.093
0.731AspHis: 0.731 ± 0.039
5.237AspIle: 5.237 ± 0.112
4.791AspLys: 4.791 ± 0.115
4.559AspLeu: 4.559 ± 0.097
1.753AspMet: 1.753 ± 0.049
2.693AspAsn: 2.693 ± 0.086
1.622AspPro: 1.622 ± 0.053
0.942AspGln: 0.942 ± 0.04
2.433AspArg: 2.433 ± 0.071
3.144AspSer: 3.144 ± 0.069
2.641AspThr: 2.641 ± 0.071
3.953AspVal: 3.953 ± 0.097
0.445AspTrp: 0.445 ± 0.03
2.606AspTyr: 2.606 ± 0.082
0.0AspXaa: 0.0 ± 0.0
Glu
6.11GluAla: 6.11 ± 0.117
0.621GluCys: 0.621 ± 0.032
4.23GluAsp: 4.23 ± 0.088
6.513GluGlu: 6.513 ± 0.148
2.992GluPhe: 2.992 ± 0.071
4.601GluGly: 4.601 ± 0.095
1.204GluHis: 1.204 ± 0.049
6.757GluIle: 6.757 ± 0.124
7.361GluLys: 7.361 ± 0.132
6.443GluLeu: 6.443 ± 0.11
2.325GluMet: 2.325 ± 0.068
4.56GluAsn: 4.56 ± 0.093
1.786GluPro: 1.786 ± 0.063
1.904GluGln: 1.904 ± 0.061
3.288GluArg: 3.288 ± 0.088
3.436GluSer: 3.436 ± 0.074
3.229GluThr: 3.229 ± 0.069
4.49GluVal: 4.49 ± 0.107
0.493GluTrp: 0.493 ± 0.034
2.813GluTyr: 2.813 ± 0.082
0.0GluXaa: 0.0 ± 0.0
Phe
3.085PheAla: 3.085 ± 0.075
0.573PheCys: 0.573 ± 0.032
2.728PheAsp: 2.728 ± 0.082
2.724PheGlu: 2.724 ± 0.068
2.108PhePhe: 2.108 ± 0.073
3.146PheGly: 3.146 ± 0.088
0.621PheHis: 0.621 ± 0.034
3.413PheIle: 3.413 ± 0.087
2.939PheLys: 2.939 ± 0.069
3.578PheLeu: 3.578 ± 0.092
1.164PheMet: 1.164 ± 0.056
2.106PheAsn: 2.106 ± 0.067
1.415PhePro: 1.415 ± 0.055
1.027PheGln: 1.027 ± 0.049
1.614PheArg: 1.614 ± 0.061
2.978PheSer: 2.978 ± 0.086
2.484PheThr: 2.484 ± 0.079
2.935PheVal: 2.935 ± 0.079
0.347PheTrp: 0.347 ± 0.026
1.509PheTyr: 1.509 ± 0.051
0.0PheXaa: 0.0 ± 0.0
Gly
5.255GlyAla: 5.255 ± 0.118
0.986GlyCys: 0.986 ± 0.054
4.053GlyAsp: 4.053 ± 0.092
4.98GlyGlu: 4.98 ± 0.109
3.371GlyPhe: 3.371 ± 0.085
5.192GlyGly: 5.192 ± 0.137
1.191GlyHis: 1.191 ± 0.055
6.838GlyIle: 6.838 ± 0.119
6.238GlyLys: 6.238 ± 0.111
6.118GlyLeu: 6.118 ± 0.129
2.144GlyMet: 2.144 ± 0.062
3.491GlyAsn: 3.491 ± 0.085
1.387GlyPro: 1.387 ± 0.053
1.853GlyGln: 1.853 ± 0.053
3.12GlyArg: 3.12 ± 0.083
4.287GlySer: 4.287 ± 0.103
4.095GlyThr: 4.095 ± 0.091
5.17GlyVal: 5.17 ± 0.112
0.593GlyTrp: 0.593 ± 0.035
3.142GlyTyr: 3.142 ± 0.069
0.0GlyXaa: 0.0 ± 0.0
His
0.807HisAla: 0.807 ± 0.037
0.235HisCys: 0.235 ± 0.021
0.772HisAsp: 0.772 ± 0.039
0.972HisGlu: 0.972 ± 0.041
0.652HisPhe: 0.652 ± 0.037
1.28HisGly: 1.28 ± 0.058
0.36HisHis: 0.36 ± 0.036
1.385HisIle: 1.385 ± 0.047
1.162HisLys: 1.162 ± 0.041
1.361HisLeu: 1.361 ± 0.055
0.512HisMet: 0.512 ± 0.029
0.755HisAsn: 0.755 ± 0.036
0.689HisPro: 0.689 ± 0.036
0.423HisGln: 0.423 ± 0.03
0.695HisArg: 0.695 ± 0.033
0.946HisSer: 0.946 ± 0.041
0.783HisThr: 0.783 ± 0.041
0.936HisVal: 0.936 ± 0.04
0.148HisTrp: 0.148 ± 0.016
0.613HisTyr: 0.613 ± 0.035
0.0HisXaa: 0.0 ± 0.0
Ile
6.513IleAla: 6.513 ± 0.13
1.195IleCys: 1.195 ± 0.052
5.041IleAsp: 5.041 ± 0.106
6.249IleGlu: 6.249 ± 0.108
3.644IlePhe: 3.644 ± 0.094
5.903IleGly: 5.903 ± 0.126
1.202IleHis: 1.202 ± 0.049
6.927IleIle: 6.927 ± 0.157
6.391IleLys: 6.391 ± 0.131
7.327IleLeu: 7.327 ± 0.133
2.318IleMet: 2.318 ± 0.07
3.997IleAsn: 3.997 ± 0.085
3.146IlePro: 3.146 ± 0.072
1.781IleGln: 1.781 ± 0.053
3.24IleArg: 3.24 ± 0.076
6.134IleSer: 6.134 ± 0.14
4.35IleThr: 4.35 ± 0.094
5.628IleVal: 5.628 ± 0.115
0.526IleTrp: 0.526 ± 0.033
2.922IleTyr: 2.922 ± 0.079
0.0IleXaa: 0.0 ± 0.0
Lys
6.354LysAla: 6.354 ± 0.146
0.648LysCys: 0.648 ± 0.044
4.852LysAsp: 4.852 ± 0.101
7.132LysGlu: 7.132 ± 0.123
2.599LysPhe: 2.599 ± 0.064
5.072LysGly: 5.072 ± 0.095
1.134LysHis: 1.134 ± 0.041
6.171LysIle: 6.171 ± 0.117
6.923LysLys: 6.923 ± 0.127
6.807LysLeu: 6.807 ± 0.119
2.324LysMet: 2.324 ± 0.063
4.206LysAsn: 4.206 ± 0.109
2.425LysPro: 2.425 ± 0.076
2.024LysGln: 2.024 ± 0.064
3.225LysArg: 3.225 ± 0.08
4.655LysSer: 4.655 ± 0.099
4.259LysThr: 4.259 ± 0.104
5.12LysVal: 5.12 ± 0.117
0.624LysTrp: 0.624 ± 0.036
3.268LysTyr: 3.268 ± 0.087
0.0LysXaa: 0.0 ± 0.0
Leu
6.45LeuAla: 6.45 ± 0.12
1.162LeuCys: 1.162 ± 0.052
4.945LeuAsp: 4.945 ± 0.098
6.415LeuGlu: 6.415 ± 0.128
3.461LeuPhe: 3.461 ± 0.094
6.291LeuGly: 6.291 ± 0.135
1.215LeuHis: 1.215 ± 0.049
7.111LeuIle: 7.111 ± 0.119
7.019LeuLys: 7.019 ± 0.118
7.73LeuLeu: 7.73 ± 0.133
2.691LeuMet: 2.691 ± 0.077
4.228LeuAsn: 4.228 ± 0.099
3.273LeuPro: 3.273 ± 0.091
2.104LeuGln: 2.104 ± 0.064
3.659LeuArg: 3.659 ± 0.081
6.472LeuSer: 6.472 ± 0.133
4.603LeuThr: 4.603 ± 0.088
5.454LeuVal: 5.454 ± 0.1
0.707LeuTrp: 0.707 ± 0.044
2.885LeuTyr: 2.885 ± 0.075
0.0LeuXaa: 0.0 ± 0.0
Met
2.612MetAla: 2.612 ± 0.073
0.275MetCys: 0.275 ± 0.021
1.77MetAsp: 1.77 ± 0.063
2.305MetGlu: 2.305 ± 0.065
0.997MetPhe: 0.997 ± 0.042
2.228MetGly: 2.228 ± 0.06
0.384MetHis: 0.384 ± 0.025
2.167MetIle: 2.167 ± 0.066
2.51MetLys: 2.51 ± 0.067
2.519MetLeu: 2.519 ± 0.064
0.831MetMet: 0.831 ± 0.04
1.498MetAsn: 1.498 ± 0.051
1.066MetPro: 1.066 ± 0.042
0.75MetGln: 0.75 ± 0.04
1.13MetArg: 1.13 ± 0.051
1.858MetSer: 1.858 ± 0.057
1.705MetThr: 1.705 ± 0.057
1.91MetVal: 1.91 ± 0.055
0.203MetTrp: 0.203 ± 0.021
0.818MetTyr: 0.818 ± 0.035
0.0MetXaa: 0.0 ± 0.0
Asn
2.843AsnAla: 2.843 ± 0.071
0.549AsnCys: 0.549 ± 0.033
2.294AsnAsp: 2.294 ± 0.07
3.136AsnGlu: 3.136 ± 0.085
2.043AsnPhe: 2.043 ± 0.074
3.556AsnGly: 3.556 ± 0.097
0.82AsnHis: 0.82 ± 0.042
4.341AsnIle: 4.341 ± 0.095
3.825AsnLys: 3.825 ± 0.093
4.586AsnLeu: 4.586 ± 0.094
1.365AsnMet: 1.365 ± 0.048
2.314AsnAsn: 2.314 ± 0.077
2.168AsnPro: 2.168 ± 0.067
1.26AsnGln: 1.26 ± 0.048
2.194AsnArg: 2.194 ± 0.068
2.898AsnSer: 2.898 ± 0.078
2.309AsnThr: 2.309 ± 0.067
3.17AsnVal: 3.17 ± 0.075
0.445AsnTrp: 0.445 ± 0.032
1.903AsnTyr: 1.903 ± 0.064
0.0AsnXaa: 0.0 ± 0.0
Pro
2.091ProAla: 2.091 ± 0.067
0.408ProCys: 0.408 ± 0.028
1.971ProAsp: 1.971 ± 0.072
2.955ProGlu: 2.955 ± 0.082
1.396ProPhe: 1.396 ± 0.053
2.276ProGly: 2.276 ± 0.069
0.556ProHis: 0.556 ± 0.034
2.606ProIle: 2.606 ± 0.076
2.268ProLys: 2.268 ± 0.068
2.61ProLeu: 2.61 ± 0.062
0.89ProMet: 0.89 ± 0.041
1.38ProAsn: 1.38 ± 0.053
0.731ProPro: 0.731 ± 0.034
0.914ProGln: 0.914 ± 0.05
0.983ProArg: 0.983 ± 0.042
1.821ProSer: 1.821 ± 0.056
1.742ProThr: 1.742 ± 0.055
2.519ProVal: 2.519 ± 0.065
0.292ProTrp: 0.292 ± 0.025
1.225ProTyr: 1.225 ± 0.05
0.0ProXaa: 0.0 ± 0.0
Gln
1.679GlnAla: 1.679 ± 0.062
0.264GlnCys: 0.264 ± 0.021
1.153GlnAsp: 1.153 ± 0.047
1.624GlnGlu: 1.624 ± 0.055
0.986GlnPhe: 0.986 ± 0.045
1.818GlnGly: 1.818 ± 0.06
0.368GlnHis: 0.368 ± 0.025
2.037GlnIle: 2.037 ± 0.065
1.901GlnLys: 1.901 ± 0.061
2.425GlnLeu: 2.425 ± 0.074
0.816GlnMet: 0.816 ± 0.038
1.117GlnAsn: 1.117 ± 0.052
0.7GlnPro: 0.7 ± 0.045
0.669GlnGln: 0.669 ± 0.044
1.09GlnArg: 1.09 ± 0.044
1.456GlnSer: 1.456 ± 0.061
1.123GlnThr: 1.123 ± 0.047
1.773GlnVal: 1.773 ± 0.055
0.249GlnTrp: 0.249 ± 0.022
0.879GlnTyr: 0.879 ± 0.04
0.0GlnXaa: 0.0 ± 0.0
Arg
2.756ArgAla: 2.756 ± 0.073
0.465ArgCys: 0.465 ± 0.03
2.314ArgAsp: 2.314 ± 0.067
3.373ArgGlu: 3.373 ± 0.082
1.635ArgPhe: 1.635 ± 0.054
2.59ArgGly: 2.59 ± 0.073
0.761ArgHis: 0.761 ± 0.036
3.362ArgIle: 3.362 ± 0.085
3.428ArgLys: 3.428 ± 0.094
3.872ArgLeu: 3.872 ± 0.095
1.304ArgMet: 1.304 ± 0.049
2.143ArgAsn: 2.143 ± 0.068
1.166ArgPro: 1.166 ± 0.048
1.219ArgGln: 1.219 ± 0.044
2.061ArgArg: 2.061 ± 0.077
1.914ArgSer: 1.914 ± 0.061
1.949ArgThr: 1.949 ± 0.059
2.682ArgVal: 2.682 ± 0.075
0.336ArgTrp: 0.336 ± 0.025
1.535ArgTyr: 1.535 ± 0.058
0.0ArgXaa: 0.0 ± 0.0
Ser
4.062SerAla: 4.062 ± 0.086
0.726SerCys: 0.726 ± 0.04
3.292SerAsp: 3.292 ± 0.072
3.999SerGlu: 3.999 ± 0.086
2.944SerPhe: 2.944 ± 0.072
5.124SerGly: 5.124 ± 0.091
1.082SerHis: 1.082 ± 0.043
5.024SerIle: 5.024 ± 0.114
4.575SerLys: 4.575 ± 0.111
5.445SerLeu: 5.445 ± 0.118
1.838SerMet: 1.838 ± 0.057
2.689SerAsn: 2.689 ± 0.094
1.884SerPro: 1.884 ± 0.064
1.624SerGln: 1.624 ± 0.059
2.532SerArg: 2.532 ± 0.079
3.659SerSer: 3.659 ± 0.107
2.907SerThr: 2.907 ± 0.079
3.812SerVal: 3.812 ± 0.08
0.501SerTrp: 0.501 ± 0.032
2.194SerTyr: 2.194 ± 0.062
0.0SerXaa: 0.0 ± 0.0
Thr
4.03ThrAla: 4.03 ± 0.091
0.602ThrCys: 0.602 ± 0.037
3.066ThrAsp: 3.066 ± 0.093
3.303ThrGlu: 3.303 ± 0.078
2.084ThrPhe: 2.084 ± 0.065
4.536ThrGly: 4.536 ± 0.091
0.811ThrHis: 0.811 ± 0.041
4.193ThrIle: 4.193 ± 0.1
3.53ThrLys: 3.53 ± 0.086
4.791ThrLeu: 4.791 ± 0.096
1.354ThrMet: 1.354 ± 0.049
1.949ThrAsn: 1.949 ± 0.073
2.065ThrPro: 2.065 ± 0.063
1.068ThrGln: 1.068 ± 0.048
1.729ThrArg: 1.729 ± 0.051
2.784ThrSer: 2.784 ± 0.072
2.719ThrThr: 2.719 ± 0.081
4.276ThrVal: 4.276 ± 0.101
0.392ThrTrp: 0.392 ± 0.026
1.827ThrTyr: 1.827 ± 0.065
0.0ThrXaa: 0.0 ± 0.0
Val
4.79ValAla: 4.79 ± 0.111
0.835ValCys: 0.835 ± 0.043
4.163ValAsp: 4.163 ± 0.097
4.897ValGlu: 4.897 ± 0.101
2.998ValPhe: 2.998 ± 0.08
4.812ValGly: 4.812 ± 0.097
0.94ValHis: 0.94 ± 0.042
5.948ValIle: 5.948 ± 0.113
5.351ValLys: 5.351 ± 0.107
6.082ValLeu: 6.082 ± 0.131
1.877ValMet: 1.877 ± 0.054
3.21ValAsn: 3.21 ± 0.077
2.348ValPro: 2.348 ± 0.075
1.404ValGln: 1.404 ± 0.051
2.567ValArg: 2.567 ± 0.076
4.361ValSer: 4.361 ± 0.089
3.783ValThr: 3.783 ± 0.105
4.921ValVal: 4.921 ± 0.107
0.51ValTrp: 0.51 ± 0.035
2.325ValTyr: 2.325 ± 0.065
0.0ValXaa: 0.0 ± 0.0
Trp
0.484TrpAla: 0.484 ± 0.029
0.1TrpCys: 0.1 ± 0.014
0.488TrpAsp: 0.488 ± 0.031
0.532TrpGlu: 0.532 ± 0.032
0.325TrpPhe: 0.325 ± 0.03
0.604TrpGly: 0.604 ± 0.039
0.17TrpHis: 0.17 ± 0.018
0.6TrpIle: 0.6 ± 0.036
0.582TrpLys: 0.582 ± 0.034
0.717TrpLeu: 0.717 ± 0.035
0.246TrpMet: 0.246 ± 0.022
0.43TrpAsn: 0.43 ± 0.034
0.205TrpPro: 0.205 ± 0.02
0.281TrpGln: 0.281 ± 0.023
0.281TrpArg: 0.281 ± 0.024
0.408TrpSer: 0.408 ± 0.032
0.401TrpThr: 0.401 ± 0.029
0.449TrpVal: 0.449 ± 0.029
0.068TrpTrp: 0.068 ± 0.012
0.345TrpTyr: 0.345 ± 0.024
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.348TyrAla: 2.348 ± 0.067
0.506TyrCys: 0.506 ± 0.032
2.414TyrAsp: 2.414 ± 0.071
2.699TyrGlu: 2.699 ± 0.076
1.797TyrPhe: 1.797 ± 0.061
3.011TyrGly: 3.011 ± 0.076
0.615TyrHis: 0.615 ± 0.035
3.086TyrIle: 3.086 ± 0.08
2.765TyrLys: 2.765 ± 0.088
3.04TyrLeu: 3.04 ± 0.081
0.988TyrMet: 0.988 ± 0.044
1.812TyrAsn: 1.812 ± 0.067
1.123TyrPro: 1.123 ± 0.043
0.864TyrGln: 0.864 ± 0.045
1.734TyrArg: 1.734 ± 0.058
2.305TyrSer: 2.305 ± 0.065
1.956TyrThr: 1.956 ± 0.068
2.372TyrVal: 2.372 ± 0.062
0.318TyrTrp: 0.318 ± 0.027
1.454TyrTyr: 1.454 ± 0.065
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1688 proteins (541392 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski