Asanoa hainanensis
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8104 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A239GE25|A0A239GE25_9ACTN Uncharacterized protein OS=Asanoa hainanensis OX=560556 GN=SAMN05421812_101356 PE=4 SV=1
MM1 pKa = 7.62 SDD3 pKa = 2.47 HH4 pKa = 6.03 WTWDD8 pKa = 3.48 DD9 pKa = 3.05 GHH11 pKa = 7.49 ADD13 pKa = 3.73 HH14 pKa = 7.48 GDD16 pKa = 3.78 HH17 pKa = 7.36 GDD19 pKa = 3.33 HH20 pKa = 7.2 LEE22 pKa = 4.09 DD23 pKa = 4.39 HH24 pKa = 6.88 GSHH27 pKa = 7.31 DD28 pKa = 3.41 EE29 pKa = 4.07 HH30 pKa = 8.48 GGYY33 pKa = 10.08 EE34 pKa = 4.1 EE35 pKa = 5.11 PEE37 pKa = 4.13 FDD39 pKa = 5.03 SGADD43 pKa = 3.33 TDD45 pKa = 4.17 PFGVEE50 pKa = 4.69 LGDD53 pKa = 3.56 HH54 pKa = 6.6 HH55 pKa = 7.39 LATHH59 pKa = 7.51 DD60 pKa = 4.96 DD61 pKa = 3.76 HH62 pKa = 9.12 QEE64 pKa = 3.64 WDD66 pKa = 4.13 DD67 pKa = 5.43 DD68 pKa = 4.45 SGADD72 pKa = 5.09 DD73 pKa = 4.68 PFAPHH78 pKa = 6.87 ALVSEE83 pKa = 4.18 QHH85 pKa = 6.32 DD86 pKa = 3.57 WAAGLDD92 pKa = 3.84 ALDD95 pKa = 5.45 ADD97 pKa = 4.69 DD98 pKa = 5.22 TPAPLDD104 pKa = 3.44 ATGLFGLDD112 pKa = 3.96 PDD114 pKa = 4.0 VSAYY118 pKa = 9.53 TDD120 pKa = 3.82 ALWPTHH126 pKa = 6.19 EE127 pKa = 5.09 FPAALDD133 pKa = 3.98 LGADD137 pKa = 3.62 LPEE140 pKa = 4.83 PVDD143 pKa = 3.66 GAPWTDD149 pKa = 3.39 PATLGTLGPADD160 pKa = 4.96 LMPSDD165 pKa = 4.17 VPEE168 pKa = 4.45 PTDD171 pKa = 3.36 LAAYY175 pKa = 9.88 AGLDD179 pKa = 3.64 PASSGWEE186 pKa = 3.85 TLLGSEE192 pKa = 5.15 DD193 pKa = 3.85 PATAALARR201 pKa = 11.84 FWGPPSAA208 pKa = 4.97
Molecular weight: 21.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.687
IPC2_protein 3.617
IPC_protein 3.656
Toseland 3.427
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.63
Rodwell 3.478
Grimsley 3.325
Solomon 3.668
Lehninger 3.617
Nozaki 3.783
DTASelect 4.075
Thurlkill 3.49
EMBOSS 3.643
Sillero 3.783
Patrickios 1.202
IPC_peptide 3.656
IPC2_peptide 3.757
IPC2.peptide.svr19 3.722
Protein with the highest isoelectric point:
>tr|A0A239LMV0|A0A239LMV0_9ACTN Pimeloyl-ACP methyl ester carboxylesterase OS=Asanoa hainanensis OX=560556 GN=SAMN05421812_104467 PE=4 SV=1
MM1 pKa = 7.65 KK2 pKa = 10.38 VRR4 pKa = 11.84 TSLRR8 pKa = 11.84 SLARR12 pKa = 11.84 KK13 pKa = 9.0 PGAKK17 pKa = 8.57 IVRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 GRR24 pKa = 11.84 IMVLNRR30 pKa = 11.84 KK31 pKa = 8.1 NPRR34 pKa = 11.84 FSGRR38 pKa = 11.84 QGG40 pKa = 2.96
Molecular weight: 4.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.954
ProMoST 13.451
Dawson 12.954
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.618
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.34
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.165
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8104
0
8104
2630270
24
3842
324.6
34.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.062 ± 0.041
0.68 ± 0.007
6.209 ± 0.024
4.918 ± 0.026
2.884 ± 0.015
9.277 ± 0.027
2.069 ± 0.013
3.451 ± 0.019
1.805 ± 0.02
10.363 ± 0.034
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.646 ± 0.01
1.939 ± 0.019
6.093 ± 0.026
2.643 ± 0.014
7.992 ± 0.033
5.027 ± 0.024
6.168 ± 0.025
9.05 ± 0.029
1.632 ± 0.01
2.092 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here