Prauserella shujinwangii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Prauserella

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5884 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T0M078|A0A2T0M078_9PSEU TetR_C_36 domain-containing protein OS=Prauserella shujinwangii OX=1453103 GN=B0I33_102107 PE=4 SV=1
MM1 pKa = 7.75SAPQPPNQPWGGEE14 pKa = 3.95QPPQGSPGGPQPEE27 pKa = 4.35QQDD30 pKa = 3.87SAFDD34 pKa = 3.87SPEE37 pKa = 3.84PTQVVQPQQSQQGEE51 pKa = 4.33QQFGDD56 pKa = 3.98SPEE59 pKa = 4.13PTQVVQPGAGQGTSGQGEE77 pKa = 4.6GGPGAEE83 pKa = 4.46STQLVPPGSQPASGIPYY100 pKa = 9.81APPPSAADD108 pKa = 3.2NPAAVNPQGGFGQPGGFDD126 pKa = 3.51PQQGGFGQQQGGFGQPGFGGPPPGQPGQPGQPGQPGAFGGQPGGFDD172 pKa = 3.55PQQQGGFGQPGFGAPPQQPGFGPGPYY198 pKa = 9.55GAPPPGFPPQQGPSGGGGNPLFGWIAAGVVSVLGLVAVIFSLIDD242 pKa = 3.41VTDD245 pKa = 3.4VTGDD249 pKa = 3.12ITGGAGVSEE258 pKa = 4.36EE259 pKa = 4.14QIEE262 pKa = 4.28QAFEE266 pKa = 3.84QLGFASIGTLRR277 pKa = 11.84FYY279 pKa = 11.04FIMVLVGSLLALAGGVFIALAGKK302 pKa = 9.86LGPVFKK308 pKa = 10.44KK309 pKa = 10.03IAPIVIAAGGVLMALFAVLIMAGTTVSDD337 pKa = 3.55EE338 pKa = 3.69ARR340 pKa = 11.84NRR342 pKa = 11.84FGDD345 pKa = 3.67AVDD348 pKa = 4.65DD349 pKa = 4.23LGGPGTVWLYY359 pKa = 11.16LVFGIIFVAVGVLGLIPATAHH380 pKa = 5.07YY381 pKa = 9.94VGLGGGQGGPGGPGGFTGPPSGGFPPPQPGFGQPGQPGGYY421 pKa = 8.46GQPGGFGQPGQPGQPGQPGGPNPSSGGFPQPGQPGGYY458 pKa = 8.98GAPPPGQPGGFGQPGQPPQQPGGYY482 pKa = 9.21GPPSHH487 pKa = 7.42PGGFGQPGQPPQQPGQPPQQWW508 pKa = 2.9

Molecular weight: 49.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T0LV94|A0A2T0LV94_9PSEU Conjugative relaxase-like TrwC/TraI family protein OS=Prauserella shujinwangii OX=1453103 GN=B0I33_105326 PE=4 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.53GKK5 pKa = 8.66RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84THH19 pKa = 5.69GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AILAARR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.35GRR42 pKa = 11.84QRR44 pKa = 11.84LSAA47 pKa = 4.04

Molecular weight: 5.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5884

0

5884

1850372

30

4488

314.5

33.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.647 ± 0.052

0.751 ± 0.009

5.976 ± 0.027

5.975 ± 0.03

2.807 ± 0.019

9.616 ± 0.033

2.258 ± 0.015

3.088 ± 0.02

1.647 ± 0.021

10.637 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.591 ± 0.012

1.683 ± 0.015

6.039 ± 0.023

2.501 ± 0.018

8.752 ± 0.032

4.698 ± 0.016

5.839 ± 0.023

9.047 ± 0.026

1.488 ± 0.014

1.961 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski