Geodermatophilus sp. LHW52908
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4784 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A372IY23|A0A372IY23_9ACTN Uncharacterized protein OS=Geodermatophilus sp. LHW52908 OX=2303986 GN=D0Z06_18850 PE=4 SV=1
MM1 pKa = 7.37 RR2 pKa = 11.84 HH3 pKa = 4.91 VLSRR7 pKa = 11.84 AIAAAAVGSVLLAGCSSTVTGTASAARR34 pKa = 11.84 PVTDD38 pKa = 3.3 VTAEE42 pKa = 3.95 EE43 pKa = 4.34 FLVTGATDD51 pKa = 3.57 SDD53 pKa = 3.08 IDD55 pKa = 3.75 RR56 pKa = 11.84 YY57 pKa = 10.77 ARR59 pKa = 11.84 NALADD64 pKa = 3.64 LTTFWEE70 pKa = 3.95 QAYY73 pKa = 9.68 PEE75 pKa = 4.87 FYY77 pKa = 10.99 GEE79 pKa = 4.84 PLTPLQGGYY88 pKa = 10.49 FSVDD92 pKa = 2.74 ADD94 pKa = 4.93 DD95 pKa = 4.66 IDD97 pKa = 3.8 EE98 pKa = 4.29 SRR100 pKa = 11.84 YY101 pKa = 9.21 PPTGVGCARR110 pKa = 11.84 LPIDD114 pKa = 3.62 PAEE117 pKa = 4.19 VEE119 pKa = 4.39 GNAFYY124 pKa = 10.91 EE125 pKa = 4.44 LNCDD129 pKa = 3.79 VIVYY133 pKa = 9.96 DD134 pKa = 4.69 SSLLQEE140 pKa = 4.68 LGEE143 pKa = 3.94 LHH145 pKa = 7.03 GRR147 pKa = 11.84 FLGPAVMAHH156 pKa = 6.07 EE157 pKa = 5.65 FGHH160 pKa = 6.59 AMQGRR165 pKa = 11.84 FGFSEE170 pKa = 3.89 VTIRR174 pKa = 11.84 DD175 pKa = 3.63 EE176 pKa = 4.19 TQADD180 pKa = 4.0 CFAGAFTRR188 pKa = 11.84 WVVDD192 pKa = 3.6 GNAEE196 pKa = 4.07 HH197 pKa = 6.23 VALRR201 pKa = 11.84 VSEE204 pKa = 4.5 LDD206 pKa = 3.56 DD207 pKa = 3.49 VLLGFIEE214 pKa = 4.44 LRR216 pKa = 11.84 DD217 pKa = 3.81 PVGLTDD223 pKa = 3.54 EE224 pKa = 4.59 TVEE227 pKa = 4.27 GAHH230 pKa = 6.45 GSGFDD235 pKa = 3.47 RR236 pKa = 11.84 VSGFYY241 pKa = 10.3 AGYY244 pKa = 7.7 TGGVGTCRR252 pKa = 11.84 DD253 pKa = 3.38 EE254 pKa = 4.58 FGPEE258 pKa = 3.61 RR259 pKa = 11.84 VFTAAEE265 pKa = 3.61 FDD267 pKa = 3.77 EE268 pKa = 5.99 AEE270 pKa = 4.27 EE271 pKa = 4.06 ATEE274 pKa = 4.36 GNADD278 pKa = 3.5 YY279 pKa = 11.62 ADD281 pKa = 3.26 IQEE284 pKa = 4.27 IVEE287 pKa = 4.13 LSLPAFYY294 pKa = 10.61 DD295 pKa = 3.1 SWFPQVGGAAFDD307 pKa = 3.87 APAIEE312 pKa = 4.97 PFDD315 pKa = 3.89 GTAPACGDD323 pKa = 3.36 MGAEE327 pKa = 4.0 DD328 pKa = 4.67 RR329 pKa = 11.84 DD330 pKa = 4.07 LGFCAADD337 pKa = 3.29 STVYY341 pKa = 10.97 ADD343 pKa = 3.71 EE344 pKa = 4.61 TDD346 pKa = 3.81 LLRR349 pKa = 11.84 PAYY352 pKa = 10.25 QEE354 pKa = 4.17 FGDD357 pKa = 4.22 FAVATAISLPYY368 pKa = 9.77 AQAARR373 pKa = 11.84 AQLGLSTDD381 pKa = 3.89 DD382 pKa = 3.48 SAGTISTVCLTGWYY396 pKa = 9.59 AARR399 pKa = 11.84 FFDD402 pKa = 5.5 GDD404 pKa = 3.59 FADD407 pKa = 4.55 TEE409 pKa = 4.38 LSPGDD414 pKa = 3.47 VDD416 pKa = 4.06 EE417 pKa = 6.64 AILFLLGHH425 pKa = 6.05 GQDD428 pKa = 4.95 DD429 pKa = 4.02 SVLPNVEE436 pKa = 3.89 LSGFEE441 pKa = 3.75 LVGAFRR447 pKa = 11.84 DD448 pKa = 4.01 GFLQGGTACDD458 pKa = 3.73 LGVV461 pKa = 3.23
Molecular weight: 49.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.696
IPC2_protein 3.783
IPC_protein 3.808
Toseland 3.592
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.935
Wikipedia 3.706
Rodwell 3.63
Grimsley 3.503
Solomon 3.783
Lehninger 3.745
Nozaki 3.897
DTASelect 4.126
Thurlkill 3.63
EMBOSS 3.719
Sillero 3.923
Patrickios 1.151
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.821
Protein with the highest isoelectric point:
>tr|A0A372IXK3|A0A372IXK3_9ACTN Uncharacterized protein OS=Geodermatophilus sp. LHW52908 OX=2303986 GN=D0Z06_23070 PE=4 SV=1
MM1 pKa = 7.57 PHH3 pKa = 7.03 RR4 pKa = 11.84 PQPASSRR11 pKa = 11.84 TAAPRR16 pKa = 11.84 TVPRR20 pKa = 11.84 STARR24 pKa = 11.84 PSTAPPSTAPPSTAPPSTAPRR45 pKa = 11.84 STAHH49 pKa = 7.24 RR50 pKa = 11.84 STGPSPTAPRR60 pKa = 11.84 RR61 pKa = 11.84 TGPRR65 pKa = 11.84 RR66 pKa = 11.84 AGGTAPPVTPRR77 pKa = 11.84 PRR79 pKa = 11.84 RR80 pKa = 11.84 TPAPSRR86 pKa = 11.84 PRR88 pKa = 11.84 ARR90 pKa = 3.61
Molecular weight: 9.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.516
IPC2_protein 11.433
IPC_protein 13.042
Toseland 13.203
ProMoST 13.7
Dawson 13.203
Bjellqvist 13.203
Wikipedia 13.685
Rodwell 12.705
Grimsley 13.247
Solomon 13.7
Lehninger 13.598
Nozaki 13.203
DTASelect 13.203
Thurlkill 13.203
EMBOSS 13.7
Sillero 13.203
Patrickios 12.427
IPC_peptide 13.7
IPC2_peptide 12.691
IPC2.peptide.svr19 9.338
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4784
0
4784
1526184
24
2587
319.0
33.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
15.01 ± 0.054
0.698 ± 0.009
6.082 ± 0.027
5.535 ± 0.034
2.474 ± 0.022
10.026 ± 0.033
2.063 ± 0.016
2.346 ± 0.025
1.113 ± 0.019
10.761 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.486 ± 0.013
1.261 ± 0.016
6.562 ± 0.04
2.463 ± 0.019
8.817 ± 0.039
4.333 ± 0.021
5.766 ± 0.028
10.0 ± 0.04
1.512 ± 0.018
1.691 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here