Deinococcus puniceus
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2606 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A172T6X9|A0A172T6X9_9DEIO Peptidyl-prolyl cis-trans isomerase OS=Deinococcus puniceus OX=1182568 GN=SU48_02320 PE=3 SV=1
MM1 pKa = 7.45 LNVADD6 pKa = 4.73 YY7 pKa = 11.41 GDD9 pKa = 4.43 APTAYY14 pKa = 10.16 GNVAHH19 pKa = 6.85 LPVLGWNGGTLGAGTNTIFPNTNTAPTFGLATQIPPTTALLGSVVDD65 pKa = 4.24 VEE67 pKa = 4.54 RR68 pKa = 11.84 TSQANATATADD79 pKa = 3.79 DD80 pKa = 4.8 ANSIDD85 pKa = 4.98 DD86 pKa = 4.03 EE87 pKa = 5.99 DD88 pKa = 4.91 GVTMPASVMIGNVVTLPVAIGAAGLLSAWFDD119 pKa = 3.49 WNRR122 pKa = 11.84 DD123 pKa = 2.7 GDD125 pKa = 3.88 FADD128 pKa = 4.29 AGEE131 pKa = 4.84 QITSDD136 pKa = 3.11 NSVSVGTTNLSVTVPTNAVAGTTFARR162 pKa = 11.84 FRR164 pKa = 11.84 ICTAAASCNSPTGVSPSGEE183 pKa = 4.07 VEE185 pKa = 4.31 DD186 pKa = 4.36 YY187 pKa = 11.19 SLVVMPTTADD197 pKa = 3.5 LNINKK202 pKa = 8.23 TGPSTVVQGSLATYY216 pKa = 8.48 TITVWNKK223 pKa = 9.87 GPGNSTGATISDD235 pKa = 4.03 PVPSNLTNVNWTCAASDD252 pKa = 3.56 TAACGIAGGSGNTINFVSGILPVNASATAPISGSYY287 pKa = 8.53 LTLTVTGTATTSGTLINTASVTAAPGTTDD316 pKa = 3.73 PVPGNNSSSQSTTVTAAMPVQPAINLKK343 pKa = 10.16 KK344 pKa = 10.13 YY345 pKa = 9.1 VRR347 pKa = 11.84 NVTTGTAFSEE357 pKa = 4.64 TSTTARR363 pKa = 11.84 PKK365 pKa = 10.66 DD366 pKa = 3.31 IIEE369 pKa = 4.06 YY370 pKa = 10.18 CITYY374 pKa = 10.35 INAGGNAANFKK385 pKa = 9.62 LTDD388 pKa = 3.66 NVPAGMIVQLDD399 pKa = 4.27 AYY401 pKa = 10.45 AAGQGILWNSTSNTVSGNTTPAGTNLTNAPSDD433 pKa = 3.77 DD434 pKa = 3.82 AGTLTGADD442 pKa = 3.9 TLPPIDD448 pKa = 3.88 FTVHH452 pKa = 5.11 GTNNTGLLTLDD463 pKa = 4.5 LSATGLANLGKK474 pKa = 9.45 GTVCFRR480 pKa = 11.84 TQIPP484 pKa = 3.4
Molecular weight: 48.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.755
IPC2_protein 3.935
IPC_protein 3.948
Toseland 3.719
ProMoST 4.139
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.923
Rodwell 3.77
Grimsley 3.63
Solomon 3.961
Lehninger 3.91
Nozaki 4.075
DTASelect 4.38
Thurlkill 3.783
EMBOSS 3.935
Sillero 4.075
Patrickios 1.952
IPC_peptide 3.948
IPC2_peptide 4.05
IPC2.peptide.svr19 3.961
Protein with the highest isoelectric point:
>tr|A0A172TCG9|A0A172TCG9_9DEIO Transcriptional regulator OS=Deinococcus puniceus OX=1182568 GN=SU48_00050 PE=4 SV=1
MM1 pKa = 7.62 LSLPAAKK8 pKa = 9.8 PLTPARR14 pKa = 11.84 RR15 pKa = 11.84 PQPAIPRR22 pKa = 11.84 QPLTRR27 pKa = 11.84 RR28 pKa = 11.84 TTAASQTTQWPVFRR42 pKa = 11.84 PGHH45 pKa = 6.33 KK46 pKa = 9.6 LQQAHH51 pKa = 6.74 RR52 pKa = 11.84 PPHH55 pKa = 5.92 RR56 pKa = 11.84 KK57 pKa = 8.49 PRR59 pKa = 11.84 QARR62 pKa = 11.84 GARR65 pKa = 11.84 PFPP68 pKa = 4.4
Molecular weight: 7.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 12.998
ProMoST 13.495
Dawson 12.998
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.618
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.34
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.193
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2606
0
2606
801718
47
3650
307.6
33.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.726 ± 0.073
0.531 ± 0.013
5.126 ± 0.039
5.351 ± 0.058
3.169 ± 0.03
9.126 ± 0.057
2.052 ± 0.033
3.864 ± 0.043
2.757 ± 0.046
11.366 ± 0.069
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.967 ± 0.025
2.704 ± 0.038
5.729 ± 0.042
3.684 ± 0.03
6.804 ± 0.048
5.11 ± 0.035
6.369 ± 0.064
7.912 ± 0.037
1.33 ± 0.022
2.325 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here