Reptilian orthoreovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Spinareovirinae; Orthoreovirus

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W8PTM7|W8PTM7_9REOV Sigma C OS=Reptilian orthoreovirus OX=226613 GN=SC PE=4 SV=1
MM1 pKa = 7.5GNASSLVQNFNITGDD16 pKa = 3.89GNSFAPTADD25 pKa = 3.52QTSSAVPTLSLQPGILNPGGLAFKK49 pKa = 10.79LIDD52 pKa = 3.7PTKK55 pKa = 10.91DD56 pKa = 3.06PTDD59 pKa = 4.24PGALAIMTTEE69 pKa = 4.73DD70 pKa = 4.02LPEE73 pKa = 4.07LAITDD78 pKa = 4.15TNNSAGALPISGMYY92 pKa = 10.2LDD94 pKa = 5.0SKK96 pKa = 10.33PEE98 pKa = 3.87KK99 pKa = 9.17LTVVTDD105 pKa = 3.72HH106 pKa = 7.31ALEE109 pKa = 3.88QMRR112 pKa = 11.84KK113 pKa = 8.86NEE115 pKa = 4.08SAVEE119 pKa = 3.7ISRR122 pKa = 11.84PYY124 pKa = 10.16LSRR127 pKa = 11.84IAVMPQSTFFTDD139 pKa = 3.44YY140 pKa = 9.91VVKK143 pKa = 10.75IKK145 pKa = 10.68GCYY148 pKa = 8.75VGASATQASRR158 pKa = 11.84TYY160 pKa = 10.79VEE162 pKa = 4.25GPYY165 pKa = 10.6VITQSRR171 pKa = 11.84MTTYY175 pKa = 10.09MSIMATASNALSKK188 pKa = 10.16WSRR191 pKa = 11.84AVTEE195 pKa = 4.09VLNIIPTTTPYY206 pKa = 11.37GKK208 pKa = 8.09TEE210 pKa = 3.99CDD212 pKa = 2.93MRR214 pKa = 11.84SIIKK218 pKa = 10.15FLDD221 pKa = 3.25QVLPEE226 pKa = 4.11DD227 pKa = 4.09HH228 pKa = 7.12LARR231 pKa = 11.84DD232 pKa = 4.07YY233 pKa = 10.99PEE235 pKa = 4.79EE236 pKa = 3.87MAEE239 pKa = 4.21TVAKK243 pKa = 10.39INGGIRR249 pKa = 11.84WKK251 pKa = 11.03DD252 pKa = 3.51EE253 pKa = 3.96LTGKK257 pKa = 8.67ATSLAINDD265 pKa = 3.43VAAPTMATLSNTMPLAEE282 pKa = 4.56KK283 pKa = 10.87SEE285 pKa = 4.31TTTDD289 pKa = 3.47SLSLINDD296 pKa = 3.57ADD298 pKa = 4.07VDD300 pKa = 4.38LMSCDD305 pKa = 3.74RR306 pKa = 11.84PISSTVWARR315 pKa = 11.84TVEE318 pKa = 4.06PKK320 pKa = 10.48DD321 pKa = 3.43YY322 pKa = 10.54NIRR325 pKa = 11.84VLPLEE330 pKa = 3.83ASMWLRR336 pKa = 11.84TAALTANFVVSWKK349 pKa = 10.42SKK351 pKa = 10.61EE352 pKa = 4.03SDD354 pKa = 2.69ATNYY358 pKa = 9.32IKK360 pKa = 10.55IMTGARR366 pKa = 11.84VINLEE371 pKa = 3.86QTGTMTFTVDD381 pKa = 5.31LEE383 pKa = 4.31GKK385 pKa = 9.58NYY387 pKa = 7.99KK388 pKa = 7.72TTSFDD393 pKa = 4.12PNGKK397 pKa = 7.48TVGLVAMQSKK407 pKa = 9.86VPFEE411 pKa = 3.98QWTAASMVIGATMVASEE428 pKa = 4.64VLVAAQSSTPGASIIAKK445 pKa = 8.47TALTYY450 pKa = 10.25IFEE453 pKa = 4.82RR454 pKa = 11.84EE455 pKa = 4.29TIPLADD461 pKa = 3.92TEE463 pKa = 4.66VNTYY467 pKa = 9.03LFCGFVLTEE476 pKa = 4.36TPTEE480 pKa = 4.43SNYY483 pKa = 10.06PWYY486 pKa = 9.97DD487 pKa = 2.8VWDD490 pKa = 3.89ATTTLTPMTTGTVTVNGSTVNEE512 pKa = 4.16VVPTSLIGAYY522 pKa = 8.91TPTAMSAALPNDD534 pKa = 3.49AGVILQGRR542 pKa = 11.84AEE544 pKa = 4.33KK545 pKa = 10.23LAKK548 pKa = 10.22AIKK551 pKa = 10.71NEE553 pKa = 4.14DD554 pKa = 3.57DD555 pKa = 3.79SVADD559 pKa = 3.79EE560 pKa = 4.45ASTYY564 pKa = 11.13SMPIMGVLALQDD576 pKa = 3.38AGKK579 pKa = 8.21PTMRR583 pKa = 11.84AKK585 pKa = 10.46FDD587 pKa = 3.81PVGLLKK593 pKa = 10.53KK594 pKa = 10.56ASRR597 pKa = 11.84AFQMFLGNPKK607 pKa = 10.31SILDD611 pKa = 3.43MTTPVMKK618 pKa = 10.71DD619 pKa = 3.12PAVWRR624 pKa = 11.84ALAQGVRR631 pKa = 11.84DD632 pKa = 4.77GIRR635 pKa = 11.84NKK637 pKa = 10.97SMGAGVKK644 pKa = 10.46SIFDD648 pKa = 3.6KK649 pKa = 11.37VSATKK654 pKa = 10.31SVQKK658 pKa = 9.71WKK660 pKa = 10.75QGILTQIQTLYY671 pKa = 9.69PPSGEE676 pKa = 3.99

Molecular weight: 72.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W8P1A7|W8P1A7_9REOV Lambda C OS=Reptilian orthoreovirus OX=226613 GN=L2 PE=4 SV=1
MM1 pKa = 7.59GNGPSNFVNHH11 pKa = 6.27SPAEE15 pKa = 4.19TIISGLDD22 pKa = 2.97KK23 pKa = 11.26GADD26 pKa = 3.34KK27 pKa = 11.32VAGTVSNTIWEE38 pKa = 4.54VVGGVLLLLFLIAVGFGLYY57 pKa = 9.77RR58 pKa = 11.84YY59 pKa = 10.14AKK61 pKa = 9.73GRR63 pKa = 11.84RR64 pKa = 11.84EE65 pKa = 4.07KK66 pKa = 10.66KK67 pKa = 9.9RR68 pKa = 11.84EE69 pKa = 3.63LTEE72 pKa = 3.84FQKK75 pKa = 10.85RR76 pKa = 11.84FLRR79 pKa = 11.84NSYY82 pKa = 10.12RR83 pKa = 11.84LSQRR87 pKa = 11.84RR88 pKa = 11.84SLTQSPDD95 pKa = 3.32YY96 pKa = 10.85EE97 pKa = 4.4EE98 pKa = 4.15PTEE101 pKa = 4.01YY102 pKa = 10.82GITKK106 pKa = 9.05PLPPPPYY113 pKa = 8.93ATYY116 pKa = 10.94INII119 pKa = 4.03

Molecular weight: 13.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

7699

119

1290

699.9

78.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.131 ± 0.616

1.221 ± 0.142

6.092 ± 0.321

4.429 ± 0.448

3.286 ± 0.172

5.221 ± 0.383

1.766 ± 0.284

5.663 ± 0.269

4.052 ± 0.548

9.469 ± 0.272

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.312 ± 0.272

4.546 ± 0.42

5.624 ± 0.346

4.234 ± 0.241

5.611 ± 0.312

7.715 ± 0.231

7.274 ± 0.486

7.17 ± 0.408

1.611 ± 0.127

3.572 ± 0.388
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski