Bacillus phage Negev_SA

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Wbetavirus; unclassified Wbetavirus

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A288WFX1|A0A288WFX1_9CAUD Uncharacterized protein OS=Bacillus phage Negev_SA OX=1983579 PE=4 SV=1
MM1 pKa = 7.25QKK3 pKa = 10.28EE4 pKa = 4.17NQFNSHH10 pKa = 6.98EE11 pKa = 4.2ILYY14 pKa = 10.22GAALDD19 pKa = 4.29PSSIGPTLPPTPTFTVPTGPTGPTGNTGPTGITGPTGNTGPTGITGPTGNTGPTGITGPTGNTGPTGDD87 pKa = 4.17TGPTGNTGPTGPPDD101 pKa = 4.17GPTGPTGPTGPTGPPDD117 pKa = 4.17GPTGPTGPTGITGPTGDD134 pKa = 4.33TGPTGITGPTGITGPTGVTGPTGITGPTGVTGPTGITGPTGDD176 pKa = 4.33TGPTGITGPTGDD188 pKa = 4.28TGPTGDD194 pKa = 4.24TGPTGDD200 pKa = 4.24TGPTGDD206 pKa = 4.27TGPTGNTGPTGIVSVVFNQTNTSGLGTSLVVSLPAINVGINQIVKK251 pKa = 10.31LDD253 pKa = 3.74GFFTTNFISQATGTQFSIDD272 pKa = 3.64AILFLRR278 pKa = 11.84RR279 pKa = 11.84DD280 pKa = 4.47AIALTQIEE288 pKa = 4.45EE289 pKa = 4.48TEE291 pKa = 4.32TIILKK296 pKa = 9.69PVNTVSQIRR305 pKa = 11.84AQSSVTWVDD314 pKa = 3.07IPPPGIYY321 pKa = 9.04IYY323 pKa = 11.63NMILQVGGSNLSNSIIGPRR342 pKa = 11.84NLTATVINNN351 pKa = 3.35

Molecular weight: 34.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A288WFW1|A0A288WFW1_9CAUD Uncharacterized protein OS=Bacillus phage Negev_SA OX=1983579 PE=4 SV=1
MM1 pKa = 7.26RR2 pKa = 11.84TWKK5 pKa = 10.13KK6 pKa = 10.35KK7 pKa = 7.96HH8 pKa = 5.1VKK10 pKa = 9.81RR11 pKa = 11.84AFLNRR16 pKa = 11.84QKK18 pKa = 10.85EE19 pKa = 3.9IDD21 pKa = 3.67KK22 pKa = 10.74EE23 pKa = 4.02RR24 pKa = 11.84TAAAWRR30 pKa = 11.84NIFVKK35 pKa = 10.61SGIIKK40 pKa = 10.38

Molecular weight: 4.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

12142

40

1332

213.0

24.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.177 ± 0.685

0.931 ± 0.182

5.617 ± 0.266

8.401 ± 0.335

4.06 ± 0.272

6.218 ± 0.471

1.647 ± 0.191

7.083 ± 0.312

9.455 ± 0.387

8.45 ± 0.393

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.619 ± 0.172

5.189 ± 0.208

2.924 ± 0.446

4.044 ± 0.324

4.662 ± 0.24

5.625 ± 0.25

5.963 ± 0.54

6.276 ± 0.222

1.112 ± 0.154

3.55 ± 0.246
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski