Amino acid dipepetide frequency for Bacillus phage Negev_SA

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.942AlaAla: 4.942 ± 1.305
0.577AlaCys: 0.577 ± 0.312
3.377AlaAsp: 3.377 ± 0.567
5.107AlaGlu: 5.107 ± 0.809
2.883AlaPhe: 2.883 ± 0.454
4.53AlaGly: 4.53 ± 0.876
0.824AlaHis: 0.824 ± 0.286
4.695AlaIle: 4.695 ± 0.582
4.695AlaLys: 4.695 ± 0.685
5.601AlaLeu: 5.601 ± 0.78
1.235AlaMet: 1.235 ± 0.381
3.295AlaAsn: 3.295 ± 0.539
1.318AlaPro: 1.318 ± 0.316
2.224AlaGln: 2.224 ± 0.453
2.718AlaArg: 2.718 ± 0.542
3.954AlaSer: 3.954 ± 0.764
3.377AlaThr: 3.377 ± 0.847
3.624AlaVal: 3.624 ± 0.867
0.659AlaTrp: 0.659 ± 0.229
2.142AlaTyr: 2.142 ± 0.549
0.0AlaXaa: 0.0 ± 0.0
Cys
0.247CysAla: 0.247 ± 0.146
0.082CysCys: 0.082 ± 0.076
0.329CysAsp: 0.329 ± 0.134
1.071CysGlu: 1.071 ± 0.42
0.247CysPhe: 0.247 ± 0.152
0.247CysGly: 0.247 ± 0.145
0.247CysHis: 0.247 ± 0.138
0.577CysIle: 0.577 ± 0.241
0.741CysLys: 0.741 ± 0.273
0.906CysLeu: 0.906 ± 0.435
0.329CysMet: 0.329 ± 0.203
0.659CysAsn: 0.659 ± 0.227
0.577CysPro: 0.577 ± 0.244
0.329CysGln: 0.329 ± 0.135
0.494CysArg: 0.494 ± 0.205
0.659CysSer: 0.659 ± 0.238
0.412CysThr: 0.412 ± 0.154
0.412CysVal: 0.412 ± 0.154
0.0CysTrp: 0.0 ± 0.0
0.741CysTyr: 0.741 ± 0.31
0.0CysXaa: 0.0 ± 0.0
Asp
3.295AspAla: 3.295 ± 0.548
0.247AspCys: 0.247 ± 0.157
3.295AspAsp: 3.295 ± 0.431
4.612AspGlu: 4.612 ± 0.608
2.718AspPhe: 2.718 ± 0.544
3.871AspGly: 3.871 ± 0.516
0.741AspHis: 0.741 ± 0.216
4.283AspIle: 4.283 ± 0.505
6.013AspLys: 6.013 ± 0.54
4.942AspLeu: 4.942 ± 0.614
2.306AspMet: 2.306 ± 0.416
2.389AspAsn: 2.389 ± 0.473
1.318AspPro: 1.318 ± 0.392
1.812AspGln: 1.812 ± 0.428
2.636AspArg: 2.636 ± 0.407
1.894AspSer: 1.894 ± 0.383
3.212AspThr: 3.212 ± 0.615
3.624AspVal: 3.624 ± 0.435
0.659AspTrp: 0.659 ± 0.182
2.306AspTyr: 2.306 ± 0.691
0.0AspXaa: 0.0 ± 0.0
Glu
5.601GluAla: 5.601 ± 0.662
1.318GluCys: 1.318 ± 0.419
4.53GluAsp: 4.53 ± 0.656
8.319GluGlu: 8.319 ± 1.325
2.8GluPhe: 2.8 ± 0.412
4.448GluGly: 4.448 ± 0.504
1.4GluHis: 1.4 ± 0.31
7.331GluIle: 7.331 ± 0.753
8.319GluLys: 8.319 ± 0.688
8.154GluLeu: 8.154 ± 0.752
3.13GluMet: 3.13 ± 0.431
3.954GluAsn: 3.954 ± 0.602
1.4GluPro: 1.4 ± 0.402
4.283GluGln: 4.283 ± 0.804
3.542GluArg: 3.542 ± 0.613
3.624GluSer: 3.624 ± 0.692
4.695GluThr: 4.695 ± 0.587
4.283GluVal: 4.283 ± 0.577
0.741GluTrp: 0.741 ± 0.279
2.142GluTyr: 2.142 ± 0.475
0.0GluXaa: 0.0 ± 0.0
Phe
2.553PheAla: 2.553 ± 0.412
0.577PheCys: 0.577 ± 0.209
2.636PheAsp: 2.636 ± 0.485
2.636PheGlu: 2.636 ± 0.417
1.647PhePhe: 1.647 ± 0.418
2.142PheGly: 2.142 ± 0.365
0.577PheHis: 0.577 ± 0.185
2.965PheIle: 2.965 ± 0.656
3.377PheLys: 3.377 ± 0.326
3.871PheLeu: 3.871 ± 0.637
1.565PheMet: 1.565 ± 0.313
2.8PheAsn: 2.8 ± 0.525
0.906PhePro: 0.906 ± 0.282
1.235PheGln: 1.235 ± 0.351
2.224PheArg: 2.224 ± 0.375
2.306PheSer: 2.306 ± 0.417
2.224PheThr: 2.224 ± 0.522
2.059PheVal: 2.059 ± 0.416
0.412PheTrp: 0.412 ± 0.194
1.894PheTyr: 1.894 ± 0.438
0.0PheXaa: 0.0 ± 0.0
Gly
3.377GlyAla: 3.377 ± 0.581
0.494GlyCys: 0.494 ± 0.222
3.13GlyAsp: 3.13 ± 0.707
5.107GlyGlu: 5.107 ± 0.782
3.542GlyPhe: 3.542 ± 0.479
3.212GlyGly: 3.212 ± 0.596
0.824GlyHis: 0.824 ± 0.199
5.601GlyIle: 5.601 ± 1.065
5.766GlyLys: 5.766 ± 0.628
5.518GlyLeu: 5.518 ± 0.934
1.894GlyMet: 1.894 ± 0.431
2.965GlyAsn: 2.965 ± 0.651
3.789GlyPro: 3.789 ± 3.219
1.894GlyGln: 1.894 ± 0.402
1.73GlyArg: 1.73 ± 0.407
2.965GlySer: 2.965 ± 0.423
2.636GlyThr: 2.636 ± 0.426
4.53GlyVal: 4.53 ± 0.611
1.071GlyTrp: 1.071 ± 0.396
2.142GlyTyr: 2.142 ± 0.435
0.0GlyXaa: 0.0 ± 0.0
His
1.071HisAla: 1.071 ± 0.312
0.659HisCys: 0.659 ± 0.251
0.659HisAsp: 0.659 ± 0.194
1.153HisGlu: 1.153 ± 0.239
0.577HisPhe: 0.577 ± 0.205
0.577HisGly: 0.577 ± 0.184
0.329HisHis: 0.329 ± 0.177
0.906HisIle: 0.906 ± 0.27
1.071HisLys: 1.071 ± 0.317
1.894HisLeu: 1.894 ± 0.469
0.577HisMet: 0.577 ± 0.237
0.906HisAsn: 0.906 ± 0.261
0.329HisPro: 0.329 ± 0.176
0.494HisGln: 0.494 ± 0.206
1.153HisArg: 1.153 ± 0.271
0.906HisSer: 0.906 ± 0.369
1.071HisThr: 1.071 ± 0.33
0.741HisVal: 0.741 ± 0.261
0.329HisTrp: 0.329 ± 0.16
1.071HisTyr: 1.071 ± 0.258
0.0HisXaa: 0.0 ± 0.0
Ile
4.53IleAla: 4.53 ± 0.451
0.577IleCys: 0.577 ± 0.21
4.86IleAsp: 4.86 ± 0.561
6.425IleGlu: 6.425 ± 0.692
1.977IlePhe: 1.977 ± 0.397
4.201IleGly: 4.201 ± 0.654
0.824IleHis: 0.824 ± 0.261
4.036IleIle: 4.036 ± 0.624
7.166IleLys: 7.166 ± 0.991
6.26IleLeu: 6.26 ± 0.777
1.4IleMet: 1.4 ± 0.345
4.118IleAsn: 4.118 ± 0.454
3.13IlePro: 3.13 ± 0.469
2.718IleGln: 2.718 ± 0.442
4.283IleArg: 4.283 ± 0.455
3.871IleSer: 3.871 ± 0.532
4.036IleThr: 4.036 ± 0.772
3.871IleVal: 3.871 ± 0.503
0.577IleTrp: 0.577 ± 0.213
1.977IleTyr: 1.977 ± 0.409
0.0IleXaa: 0.0 ± 0.0
Lys
5.107LysAla: 5.107 ± 0.85
0.741LysCys: 0.741 ± 0.247
4.118LysAsp: 4.118 ± 0.476
9.472LysGlu: 9.472 ± 1.055
2.718LysPhe: 2.718 ± 0.464
5.354LysGly: 5.354 ± 0.646
1.565LysHis: 1.565 ± 0.376
7.001LysIle: 7.001 ± 0.752
9.307LysLys: 9.307 ± 1.104
8.401LysLeu: 8.401 ± 0.916
3.377LysMet: 3.377 ± 0.555
4.777LysAsn: 4.777 ± 0.598
2.883LysPro: 2.883 ± 0.56
3.459LysGln: 3.459 ± 0.689
4.942LysArg: 4.942 ± 0.754
4.942LysSer: 4.942 ± 0.589
5.271LysThr: 5.271 ± 0.695
6.507LysVal: 6.507 ± 0.721
1.071LysTrp: 1.071 ± 0.315
3.542LysTyr: 3.542 ± 0.604
0.0LysXaa: 0.0 ± 0.0
Leu
5.271LeuAla: 5.271 ± 0.655
0.577LeuCys: 0.577 ± 0.228
5.436LeuAsp: 5.436 ± 0.538
7.166LeuGlu: 7.166 ± 0.892
4.118LeuPhe: 4.118 ± 0.895
5.024LeuGly: 5.024 ± 0.746
1.235LeuHis: 1.235 ± 0.309
4.777LeuIle: 4.777 ± 0.732
8.566LeuLys: 8.566 ± 0.717
6.425LeuLeu: 6.425 ± 0.882
1.647LeuMet: 1.647 ± 0.282
5.271LeuAsn: 5.271 ± 0.779
1.894LeuPro: 1.894 ± 0.476
4.283LeuGln: 4.283 ± 0.698
3.459LeuArg: 3.459 ± 0.522
5.354LeuSer: 5.354 ± 0.588
5.354LeuThr: 5.354 ± 0.63
4.612LeuVal: 4.612 ± 0.72
0.906LeuTrp: 0.906 ± 0.348
3.13LeuTyr: 3.13 ± 0.604
0.0LeuXaa: 0.0 ± 0.0
Met
1.565MetAla: 1.565 ± 0.349
0.165MetCys: 0.165 ± 0.112
1.4MetAsp: 1.4 ± 0.349
2.059MetGlu: 2.059 ± 0.39
0.824MetPhe: 0.824 ± 0.408
1.4MetGly: 1.4 ± 0.38
0.906MetHis: 0.906 ± 0.305
1.894MetIle: 1.894 ± 0.37
3.13MetLys: 3.13 ± 0.528
1.565MetLeu: 1.565 ± 0.375
0.494MetMet: 0.494 ± 0.224
1.153MetAsn: 1.153 ± 0.314
0.824MetPro: 0.824 ± 0.187
0.741MetGln: 0.741 ± 0.299
1.4MetArg: 1.4 ± 0.257
1.977MetSer: 1.977 ± 0.407
1.977MetThr: 1.977 ± 0.302
1.318MetVal: 1.318 ± 0.34
0.659MetTrp: 0.659 ± 0.234
0.741MetTyr: 0.741 ± 0.262
0.0MetXaa: 0.0 ± 0.0
Asn
3.377AsnAla: 3.377 ± 0.63
0.247AsnCys: 0.247 ± 0.154
2.306AsnAsp: 2.306 ± 0.418
4.53AsnGlu: 4.53 ± 0.702
2.059AsnPhe: 2.059 ± 0.387
3.295AsnGly: 3.295 ± 0.439
1.318AsnHis: 1.318 ± 0.361
3.048AsnIle: 3.048 ± 0.556
5.189AsnLys: 5.189 ± 0.585
3.954AsnLeu: 3.954 ± 0.507
1.318AsnMet: 1.318 ± 0.385
2.306AsnAsn: 2.306 ± 0.397
1.894AsnPro: 1.894 ± 0.429
2.8AsnGln: 2.8 ± 0.413
3.295AsnArg: 3.295 ± 0.493
2.718AsnSer: 2.718 ± 0.471
2.965AsnThr: 2.965 ± 0.688
3.377AsnVal: 3.377 ± 0.556
0.741AsnTrp: 0.741 ± 0.269
1.153AsnTyr: 1.153 ± 0.391
0.0AsnXaa: 0.0 ± 0.0
Pro
1.647ProAla: 1.647 ± 0.315
0.329ProCys: 0.329 ± 0.198
1.4ProAsp: 1.4 ± 0.362
2.306ProGlu: 2.306 ± 0.438
0.988ProPhe: 0.988 ± 0.262
0.988ProGly: 0.988 ± 0.184
0.659ProHis: 0.659 ± 0.27
1.318ProIle: 1.318 ± 0.396
1.73ProLys: 1.73 ± 0.425
2.059ProLeu: 2.059 ± 0.433
0.494ProMet: 0.494 ± 0.195
1.483ProAsn: 1.483 ± 0.367
0.988ProPro: 0.988 ± 0.446
1.071ProGln: 1.071 ± 0.248
0.988ProArg: 0.988 ± 0.343
2.059ProSer: 2.059 ± 0.338
4.942ProThr: 4.942 ± 3.181
2.636ProVal: 2.636 ± 0.471
0.247ProTrp: 0.247 ± 0.201
0.906ProTyr: 0.906 ± 0.314
0.0ProXaa: 0.0 ± 0.0
Gln
2.636GlnAla: 2.636 ± 0.824
0.165GlnCys: 0.165 ± 0.123
1.565GlnAsp: 1.565 ± 0.331
3.377GlnGlu: 3.377 ± 0.549
2.059GlnPhe: 2.059 ± 0.462
2.224GlnGly: 2.224 ± 0.478
0.659GlnHis: 0.659 ± 0.232
2.471GlnIle: 2.471 ± 0.386
4.448GlnLys: 4.448 ± 0.659
2.8GlnLeu: 2.8 ± 0.535
1.153GlnMet: 1.153 ± 0.325
2.142GlnAsn: 2.142 ± 0.487
1.647GlnPro: 1.647 ± 0.319
2.306GlnGln: 2.306 ± 0.68
1.894GlnArg: 1.894 ± 0.422
2.553GlnSer: 2.553 ± 0.369
1.647GlnThr: 1.647 ± 0.377
2.883GlnVal: 2.883 ± 0.551
0.329GlnTrp: 0.329 ± 0.137
1.483GlnTyr: 1.483 ± 0.403
0.0GlnXaa: 0.0 ± 0.0
Arg
3.13ArgAla: 3.13 ± 0.454
0.329ArgCys: 0.329 ± 0.176
3.459ArgAsp: 3.459 ± 0.463
3.871ArgGlu: 3.871 ± 0.467
2.306ArgPhe: 2.306 ± 0.451
2.883ArgGly: 2.883 ± 0.477
0.741ArgHis: 0.741 ± 0.21
3.871ArgIle: 3.871 ± 0.634
3.954ArgLys: 3.954 ± 0.761
4.365ArgLeu: 4.365 ± 0.644
0.741ArgMet: 0.741 ± 0.236
2.389ArgAsn: 2.389 ± 0.533
0.988ArgPro: 0.988 ± 0.296
1.894ArgGln: 1.894 ± 0.625
1.73ArgArg: 1.73 ± 0.34
2.8ArgSer: 2.8 ± 0.624
2.224ArgThr: 2.224 ± 0.362
2.306ArgVal: 2.306 ± 0.351
0.494ArgTrp: 0.494 ± 0.204
2.142ArgTyr: 2.142 ± 0.408
0.0ArgXaa: 0.0 ± 0.0
Ser
3.377SerAla: 3.377 ± 0.715
0.412SerCys: 0.412 ± 0.2
3.789SerAsp: 3.789 ± 0.505
3.13SerGlu: 3.13 ± 0.491
2.306SerPhe: 2.306 ± 0.51
3.542SerGly: 3.542 ± 0.559
1.153SerHis: 1.153 ± 0.328
3.706SerIle: 3.706 ± 0.504
6.425SerLys: 6.425 ± 0.798
5.354SerLeu: 5.354 ± 0.697
1.318SerMet: 1.318 ± 0.362
2.8SerAsn: 2.8 ± 0.41
1.071SerPro: 1.071 ± 0.332
2.059SerGln: 2.059 ± 0.371
2.306SerArg: 2.306 ± 0.374
3.13SerSer: 3.13 ± 0.445
3.212SerThr: 3.212 ± 0.523
4.365SerVal: 4.365 ± 0.599
0.577SerTrp: 0.577 ± 0.231
2.142SerTyr: 2.142 ± 0.419
0.0SerXaa: 0.0 ± 0.0
Thr
3.542ThrAla: 3.542 ± 0.76
0.247ThrCys: 0.247 ± 0.143
2.8ThrAsp: 2.8 ± 0.4
4.365ThrGlu: 4.365 ± 0.803
2.306ThrPhe: 2.306 ± 0.461
8.237ThrGly: 8.237 ± 4.956
0.577ThrHis: 0.577 ± 0.219
4.53ThrIle: 4.53 ± 0.649
5.024ThrLys: 5.024 ± 0.664
4.448ThrLeu: 4.448 ± 0.666
1.235ThrMet: 1.235 ± 0.338
2.471ThrAsn: 2.471 ± 0.579
1.894ThrPro: 1.894 ± 0.473
2.718ThrGln: 2.718 ± 0.399
2.142ThrArg: 2.142 ± 0.365
3.212ThrSer: 3.212 ± 0.409
3.871ThrThr: 3.871 ± 0.613
3.789ThrVal: 3.789 ± 0.485
0.329ThrTrp: 0.329 ± 0.171
1.894ThrTyr: 1.894 ± 0.357
0.0ThrXaa: 0.0 ± 0.0
Val
3.871ValAla: 3.871 ± 0.603
0.577ValCys: 0.577 ± 0.258
3.954ValAsp: 3.954 ± 0.447
5.518ValGlu: 5.518 ± 0.701
2.224ValPhe: 2.224 ± 0.537
3.542ValGly: 3.542 ± 0.559
1.071ValHis: 1.071 ± 0.306
4.365ValIle: 4.365 ± 0.64
5.354ValLys: 5.354 ± 0.58
4.612ValLeu: 4.612 ± 0.851
1.318ValMet: 1.318 ± 0.348
3.212ValAsn: 3.212 ± 0.503
1.565ValPro: 1.565 ± 0.34
2.718ValGln: 2.718 ± 0.542
3.13ValArg: 3.13 ± 0.454
4.612ValSer: 4.612 ± 0.511
3.377ValThr: 3.377 ± 0.434
4.448ValVal: 4.448 ± 0.624
0.659ValTrp: 0.659 ± 0.247
2.636ValTyr: 2.636 ± 0.565
0.0ValXaa: 0.0 ± 0.0
Trp
0.412TrpAla: 0.412 ± 0.281
0.247TrpCys: 0.247 ± 0.151
0.824TrpAsp: 0.824 ± 0.24
0.906TrpGlu: 0.906 ± 0.251
0.577TrpPhe: 0.577 ± 0.216
0.577TrpGly: 0.577 ± 0.216
0.082TrpHis: 0.082 ± 0.087
0.824TrpIle: 0.824 ± 0.401
0.741TrpLys: 0.741 ± 0.256
0.988TrpLeu: 0.988 ± 0.289
0.0TrpMet: 0.0 ± 0.0
0.741TrpAsn: 0.741 ± 0.232
0.082TrpPro: 0.082 ± 0.086
0.329TrpGln: 0.329 ± 0.165
0.659TrpArg: 0.659 ± 0.228
0.988TrpSer: 0.988 ± 0.321
0.824TrpThr: 0.824 ± 0.241
0.824TrpVal: 0.824 ± 0.276
0.165TrpTrp: 0.165 ± 0.095
0.329TrpTyr: 0.329 ± 0.174
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.224TyrAla: 2.224 ± 0.434
0.741TyrCys: 0.741 ± 0.279
2.306TyrAsp: 2.306 ± 0.402
2.883TyrGlu: 2.883 ± 0.429
1.73TyrPhe: 1.73 ± 0.389
1.894TyrGly: 1.894 ± 0.381
0.741TyrHis: 0.741 ± 0.201
2.636TyrIle: 2.636 ± 0.458
3.542TyrLys: 3.542 ± 0.681
2.389TyrLeu: 2.389 ± 0.539
0.577TyrMet: 0.577 ± 0.179
2.059TyrAsn: 2.059 ± 0.415
0.741TyrPro: 0.741 ± 0.296
1.071TyrGln: 1.071 ± 0.316
1.894TyrArg: 1.894 ± 0.338
1.73TyrSer: 1.73 ± 0.339
2.306TyrThr: 2.306 ± 0.46
2.553TyrVal: 2.553 ± 0.456
0.494TyrTrp: 0.494 ± 0.198
0.988TyrTyr: 0.988 ± 0.325
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 57 proteins (12142 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski