Pectobacterium phage MA2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Corkvirinae; Kotilavirus; unclassified Kotilavirus

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 34 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2F404|A0A5Q2F404_9CAUD Uncharacterized protein OS=Pectobacterium phage MA2 OX=2608298 GN=MA2_07 PE=4 SV=1
MM1 pKa = 7.42YY2 pKa = 9.95SVQIAVSDD10 pKa = 4.2GSLSRR15 pKa = 11.84IALSIEE21 pKa = 4.04YY22 pKa = 10.05FEE24 pKa = 6.23KK25 pKa = 10.88DD26 pKa = 4.01DD27 pKa = 3.51ISLYY31 pKa = 10.61RR32 pKa = 11.84NLSTVPMVLGDD43 pKa = 3.35DD44 pKa = 3.98WQWDD48 pKa = 3.42GDD50 pKa = 3.71AHH52 pKa = 7.08INLLSDD58 pKa = 3.55VPVPNGGYY66 pKa = 7.81ITVRR70 pKa = 11.84RR71 pKa = 11.84NTNIDD76 pKa = 3.36RR77 pKa = 11.84AFNIYY82 pKa = 10.56DD83 pKa = 3.51GGAAFTRR90 pKa = 11.84DD91 pKa = 3.39TLDD94 pKa = 3.28EE95 pKa = 4.31NFKK98 pKa = 11.21QMIYY102 pKa = 10.2LAQEE106 pKa = 3.97FTEE109 pKa = 4.83GNGLTGLFFPLNMNGFKK126 pKa = 9.12ITNLGAGTDD135 pKa = 4.25DD136 pKa = 3.86KK137 pKa = 11.79DD138 pKa = 4.07AVNKK142 pKa = 10.0GQLDD146 pKa = 4.07GVSDD150 pKa = 3.82RR151 pKa = 11.84VSSLEE156 pKa = 3.88GTFVTGTTSYY166 pKa = 10.17PWYY169 pKa = 9.47TITTEE174 pKa = 3.89PTLVFSPPFLFTKK187 pKa = 10.47AALYY191 pKa = 10.83LNGVCQVPGLSYY203 pKa = 10.84SVSNNQIRR211 pKa = 11.84LATNVAPGTVVFARR225 pKa = 11.84LGEE228 pKa = 4.38DD229 pKa = 2.79TDD231 pKa = 3.88AATEE235 pKa = 4.25GVTQAQFLALMARR248 pKa = 11.84VEE250 pKa = 4.05ALEE253 pKa = 4.05NPP255 pKa = 4.01

Molecular weight: 27.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2F5A2|A0A5Q2F5A2_9CAUD Putative tubular protein A OS=Pectobacterium phage MA2 OX=2608298 GN=MA2_27 PE=4 SV=1
MM1 pKa = 7.74RR2 pKa = 11.84KK3 pKa = 9.21LARR6 pKa = 11.84SQVRR10 pKa = 11.84PTAMRR15 pKa = 11.84LLQQQGGVCPLCNKK29 pKa = 9.8PVDD32 pKa = 4.14LTEE35 pKa = 4.52KK36 pKa = 10.37GALVLDD42 pKa = 4.54HH43 pKa = 7.41DD44 pKa = 4.86HH45 pKa = 6.51GTGQVRR51 pKa = 11.84GALHH55 pKa = 6.9RR56 pKa = 11.84SCNSAEE62 pKa = 3.99GKK64 pKa = 9.22VANAAGRR71 pKa = 11.84WGAKK75 pKa = 7.3SMRR78 pKa = 11.84YY79 pKa = 9.14EE80 pKa = 4.59DD81 pKa = 3.22IVPFLEE87 pKa = 4.18RR88 pKa = 11.84LVAYY92 pKa = 9.97LRR94 pKa = 11.84QPPQDD99 pKa = 3.34MIYY102 pKa = 8.45PTFKK106 pKa = 10.29TADD109 pKa = 3.98EE110 pKa = 4.12LRR112 pKa = 11.84MARR115 pKa = 11.84NVKK118 pKa = 9.38EE119 pKa = 3.84RR120 pKa = 11.84TRR122 pKa = 11.84RR123 pKa = 11.84AEE125 pKa = 3.75RR126 pKa = 11.84KK127 pKa = 9.17ARR129 pKa = 11.84EE130 pKa = 3.83TVRR133 pKa = 11.84RR134 pKa = 11.84SRR136 pKa = 11.84VNKK139 pKa = 9.72QDD141 pKa = 3.0

Molecular weight: 16.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

34

0

34

11986

104

1230

352.5

38.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.469 ± 0.494

1.226 ± 0.185

6.616 ± 0.253

4.964 ± 0.329

3.137 ± 0.185

7.517 ± 0.234

2.127 ± 0.187

4.338 ± 0.181

4.063 ± 0.265

8.76 ± 0.274

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.953 ± 0.151

4.297 ± 0.253

4.322 ± 0.211

4.53 ± 0.433

6.015 ± 0.286

6.249 ± 0.3

6.508 ± 0.403

7.617 ± 0.228

1.285 ± 0.129

4.005 ± 0.264
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski