Amino acid dipepetide frequency for Pectobacterium phage MA2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.93AlaAla: 10.93 ± 0.954
1.001AlaCys: 1.001 ± 0.311
7.259AlaAsp: 7.259 ± 0.923
5.507AlaGlu: 5.507 ± 0.974
3.338AlaPhe: 3.338 ± 0.437
7.76AlaGly: 7.76 ± 0.871
1.919AlaHis: 1.919 ± 0.466
3.588AlaIle: 3.588 ± 0.455
3.421AlaLys: 3.421 ± 0.487
8.678AlaLeu: 8.678 ± 0.982
2.837AlaMet: 2.837 ± 0.562
3.421AlaAsn: 3.421 ± 0.577
3.504AlaPro: 3.504 ± 0.504
4.923AlaGln: 4.923 ± 0.667
4.422AlaArg: 4.422 ± 0.666
4.923AlaSer: 4.923 ± 0.537
5.257AlaThr: 5.257 ± 0.897
7.426AlaVal: 7.426 ± 0.79
1.168AlaTrp: 1.168 ± 0.239
3.421AlaTyr: 3.421 ± 0.465
0.0AlaXaa: 0.0 ± 0.0
Cys
0.751CysAla: 0.751 ± 0.296
0.25CysCys: 0.25 ± 0.188
1.252CysAsp: 1.252 ± 0.453
0.334CysGlu: 0.334 ± 0.159
0.25CysPhe: 0.25 ± 0.135
0.334CysGly: 0.334 ± 0.154
0.501CysHis: 0.501 ± 0.274
0.918CysIle: 0.918 ± 0.29
0.584CysLys: 0.584 ± 0.254
0.668CysLeu: 0.668 ± 0.229
0.751CysMet: 0.751 ± 0.243
0.501CysAsn: 0.501 ± 0.271
0.584CysPro: 0.584 ± 0.228
0.334CysGln: 0.334 ± 0.179
0.167CysArg: 0.167 ± 0.115
0.751CysSer: 0.751 ± 0.356
1.001CysThr: 1.001 ± 0.332
1.252CysVal: 1.252 ± 0.394
0.25CysTrp: 0.25 ± 0.135
0.834CysTyr: 0.834 ± 0.292
0.0CysXaa: 0.0 ± 0.0
Asp
6.842AspAla: 6.842 ± 0.73
0.834AspCys: 0.834 ± 0.254
4.422AspAsp: 4.422 ± 0.622
3.004AspGlu: 3.004 ± 0.489
1.836AspPhe: 1.836 ± 0.329
5.34AspGly: 5.34 ± 0.945
0.584AspHis: 0.584 ± 0.255
3.421AspIle: 3.421 ± 0.482
3.087AspLys: 3.087 ± 0.478
4.673AspLeu: 4.673 ± 0.564
2.253AspMet: 2.253 ± 0.337
3.504AspAsn: 3.504 ± 0.682
2.253AspPro: 2.253 ± 0.373
1.502AspGln: 1.502 ± 0.35
3.254AspArg: 3.254 ± 0.539
5.006AspSer: 5.006 ± 0.703
5.507AspThr: 5.507 ± 0.595
5.34AspVal: 5.34 ± 0.691
1.001AspTrp: 1.001 ± 0.322
2.503AspTyr: 2.503 ± 0.493
0.0AspXaa: 0.0 ± 0.0
Glu
4.422GluAla: 4.422 ± 0.577
0.25GluCys: 0.25 ± 0.16
2.587GluAsp: 2.587 ± 0.322
3.338GluGlu: 3.338 ± 0.655
2.42GluPhe: 2.42 ± 0.493
3.087GluGly: 3.087 ± 0.503
1.335GluHis: 1.335 ± 0.295
1.418GluIle: 1.418 ± 0.313
1.669GluLys: 1.669 ± 0.506
4.506GluLeu: 4.506 ± 0.661
1.585GluMet: 1.585 ± 0.386
2.503GluAsn: 2.503 ± 0.474
0.834GluPro: 0.834 ± 0.219
2.503GluGln: 2.503 ± 0.491
3.671GluArg: 3.671 ± 0.543
3.421GluSer: 3.421 ± 0.587
2.67GluThr: 2.67 ± 0.572
3.838GluVal: 3.838 ± 0.697
0.668GluTrp: 0.668 ± 0.238
2.837GluTyr: 2.837 ± 0.505
0.0GluXaa: 0.0 ± 0.0
Phe
3.254PheAla: 3.254 ± 0.432
0.083PheCys: 0.083 ± 0.08
3.087PheAsp: 3.087 ± 0.511
1.168PheGlu: 1.168 ± 0.3
1.168PhePhe: 1.168 ± 0.312
3.171PheGly: 3.171 ± 0.449
0.417PheHis: 0.417 ± 0.157
1.168PheIle: 1.168 ± 0.304
1.669PheLys: 1.669 ± 0.352
2.169PheLeu: 2.169 ± 0.306
0.834PheMet: 0.834 ± 0.188
1.669PheAsn: 1.669 ± 0.424
1.335PhePro: 1.335 ± 0.325
0.834PheGln: 0.834 ± 0.261
2.003PheArg: 2.003 ± 0.484
2.086PheSer: 2.086 ± 0.555
1.502PheThr: 1.502 ± 0.357
2.67PheVal: 2.67 ± 0.419
0.417PheTrp: 0.417 ± 0.167
0.668PheTyr: 0.668 ± 0.265
0.0PheXaa: 0.0 ± 0.0
Gly
7.676GlyAla: 7.676 ± 1.123
1.418GlyCys: 1.418 ± 0.459
4.923GlyAsp: 4.923 ± 0.697
2.336GlyGlu: 2.336 ± 0.272
2.587GlyPhe: 2.587 ± 0.311
5.423GlyGly: 5.423 ± 0.798
0.834GlyHis: 0.834 ± 0.202
5.674GlyIle: 5.674 ± 0.43
4.422GlyLys: 4.422 ± 0.712
5.59GlyLeu: 5.59 ± 0.533
2.003GlyMet: 2.003 ± 0.41
2.753GlyAsn: 2.753 ± 0.405
2.003GlyPro: 2.003 ± 0.607
2.753GlyGln: 2.753 ± 0.453
4.088GlyArg: 4.088 ± 0.501
4.172GlySer: 4.172 ± 0.645
6.174GlyThr: 6.174 ± 1.081
5.507GlyVal: 5.507 ± 0.523
0.918GlyTrp: 0.918 ± 0.278
3.922GlyTyr: 3.922 ± 0.793
0.0GlyXaa: 0.0 ± 0.0
His
1.418HisAla: 1.418 ± 0.372
0.501HisCys: 0.501 ± 0.213
1.418HisAsp: 1.418 ± 0.3
1.001HisGlu: 1.001 ± 0.339
0.25HisPhe: 0.25 ± 0.155
1.752HisGly: 1.752 ± 0.465
0.584HisHis: 0.584 ± 0.253
1.085HisIle: 1.085 ± 0.207
0.918HisLys: 0.918 ± 0.375
2.086HisLeu: 2.086 ± 0.511
0.918HisMet: 0.918 ± 0.321
0.834HisAsn: 0.834 ± 0.255
1.252HisPro: 1.252 ± 0.427
0.918HisGln: 0.918 ± 0.208
1.585HisArg: 1.585 ± 0.346
1.168HisSer: 1.168 ± 0.326
0.751HisThr: 0.751 ± 0.293
1.418HisVal: 1.418 ± 0.364
0.501HisTrp: 0.501 ± 0.226
0.918HisTyr: 0.918 ± 0.381
0.0HisXaa: 0.0 ± 0.0
Ile
4.339IleAla: 4.339 ± 0.621
0.501IleCys: 0.501 ± 0.215
2.42IleAsp: 2.42 ± 0.319
3.254IleGlu: 3.254 ± 0.594
0.584IlePhe: 0.584 ± 0.217
3.004IleGly: 3.004 ± 0.391
1.418IleHis: 1.418 ± 0.333
1.085IleIle: 1.085 ± 0.257
2.837IleLys: 2.837 ± 0.399
3.755IleLeu: 3.755 ± 0.744
1.085IleMet: 1.085 ± 0.301
1.752IleAsn: 1.752 ± 0.318
2.67IlePro: 2.67 ± 0.371
2.253IleGln: 2.253 ± 0.443
1.919IleArg: 1.919 ± 0.459
2.92IleSer: 2.92 ± 0.463
4.172IleThr: 4.172 ± 0.792
1.836IleVal: 1.836 ± 0.393
0.417IleTrp: 0.417 ± 0.198
1.168IleTyr: 1.168 ± 0.365
0.0IleXaa: 0.0 ± 0.0
Lys
4.756LysAla: 4.756 ± 0.766
0.334LysCys: 0.334 ± 0.184
2.837LysAsp: 2.837 ± 0.491
2.837LysGlu: 2.837 ± 0.53
0.918LysPhe: 0.918 ± 0.268
2.67LysGly: 2.67 ± 0.401
0.668LysHis: 0.668 ± 0.247
1.085LysIle: 1.085 ± 0.314
1.335LysLys: 1.335 ± 0.458
4.339LysLeu: 4.339 ± 0.513
1.168LysMet: 1.168 ± 0.304
1.418LysAsn: 1.418 ± 0.308
2.003LysPro: 2.003 ± 0.35
2.42LysGln: 2.42 ± 0.533
2.67LysArg: 2.67 ± 0.464
2.086LysSer: 2.086 ± 0.414
2.336LysThr: 2.336 ± 0.503
3.004LysVal: 3.004 ± 0.606
0.334LysTrp: 0.334 ± 0.201
1.418LysTyr: 1.418 ± 0.418
0.0LysXaa: 0.0 ± 0.0
Leu
7.426LeuAla: 7.426 ± 0.785
1.418LeuCys: 1.418 ± 0.383
5.257LeuAsp: 5.257 ± 0.493
4.255LeuGlu: 4.255 ± 0.551
2.587LeuPhe: 2.587 ± 0.414
6.091LeuGly: 6.091 ± 0.663
2.253LeuHis: 2.253 ± 0.331
3.338LeuIle: 3.338 ± 0.455
3.504LeuLys: 3.504 ± 0.48
7.343LeuLeu: 7.343 ± 0.799
2.92LeuMet: 2.92 ± 0.289
3.671LeuAsn: 3.671 ± 0.579
5.173LeuPro: 5.173 ± 0.523
2.92LeuGln: 2.92 ± 0.456
6.091LeuArg: 6.091 ± 0.827
6.925LeuSer: 6.925 ± 0.795
4.923LeuThr: 4.923 ± 0.53
7.676LeuVal: 7.676 ± 0.853
0.834LeuTrp: 0.834 ± 0.266
3.004LeuTyr: 3.004 ± 0.545
0.0LeuXaa: 0.0 ± 0.0
Met
3.004MetAla: 3.004 ± 0.632
0.334MetCys: 0.334 ± 0.167
1.585MetAsp: 1.585 ± 0.492
0.918MetGlu: 0.918 ± 0.24
1.168MetPhe: 1.168 ± 0.296
2.086MetGly: 2.086 ± 0.361
0.834MetHis: 0.834 ± 0.276
0.834MetIle: 0.834 ± 0.233
1.001MetLys: 1.001 ± 0.26
2.67MetLeu: 2.67 ± 0.562
0.668MetMet: 0.668 ± 0.221
0.668MetAsn: 0.668 ± 0.181
1.669MetPro: 1.669 ± 0.548
2.42MetGln: 2.42 ± 0.421
2.253MetArg: 2.253 ± 0.457
1.335MetSer: 1.335 ± 0.39
1.585MetThr: 1.585 ± 0.373
2.67MetVal: 2.67 ± 0.375
0.334MetTrp: 0.334 ± 0.155
1.502MetTyr: 1.502 ± 0.283
0.0MetXaa: 0.0 ± 0.0
Asn
4.005AsnAla: 4.005 ± 0.561
0.25AsnCys: 0.25 ± 0.137
1.669AsnAsp: 1.669 ± 0.401
1.669AsnGlu: 1.669 ± 0.293
2.003AsnPhe: 2.003 ± 0.376
3.504AsnGly: 3.504 ± 0.589
0.751AsnHis: 0.751 ± 0.267
1.919AsnIle: 1.919 ± 0.455
2.336AsnLys: 2.336 ± 0.316
4.673AsnLeu: 4.673 ± 0.603
1.836AsnMet: 1.836 ± 0.335
2.086AsnAsn: 2.086 ± 0.332
2.086AsnPro: 2.086 ± 0.526
2.003AsnGln: 2.003 ± 0.674
2.336AsnArg: 2.336 ± 0.346
2.42AsnSer: 2.42 ± 0.455
2.92AsnThr: 2.92 ± 0.445
2.92AsnVal: 2.92 ± 0.582
0.501AsnTrp: 0.501 ± 0.19
1.085AsnTyr: 1.085 ± 0.297
0.0AsnXaa: 0.0 ± 0.0
Pro
3.504ProAla: 3.504 ± 0.636
0.334ProCys: 0.334 ± 0.158
4.088ProAsp: 4.088 ± 0.589
3.171ProGlu: 3.171 ± 0.497
1.168ProPhe: 1.168 ± 0.301
2.587ProGly: 2.587 ± 0.359
0.334ProHis: 0.334 ± 0.151
1.585ProIle: 1.585 ± 0.499
1.252ProLys: 1.252 ± 0.331
3.171ProLeu: 3.171 ± 0.569
1.585ProMet: 1.585 ± 0.257
2.336ProAsn: 2.336 ± 0.456
2.169ProPro: 2.169 ± 0.519
1.836ProGln: 1.836 ± 0.505
2.003ProArg: 2.003 ± 0.419
3.004ProSer: 3.004 ± 0.444
3.671ProThr: 3.671 ± 0.615
3.254ProVal: 3.254 ± 0.403
0.417ProTrp: 0.417 ± 0.219
1.752ProTyr: 1.752 ± 0.288
0.0ProXaa: 0.0 ± 0.0
Gln
4.339GlnAla: 4.339 ± 0.735
0.417GlnCys: 0.417 ± 0.276
2.003GlnAsp: 2.003 ± 0.475
2.837GlnGlu: 2.837 ± 0.495
1.752GlnPhe: 1.752 ± 0.28
4.088GlnGly: 4.088 ± 0.567
1.585GlnHis: 1.585 ± 0.441
1.585GlnIle: 1.585 ± 0.337
0.918GlnLys: 0.918 ± 0.227
3.838GlnLeu: 3.838 ± 0.65
1.418GlnMet: 1.418 ± 0.375
2.169GlnAsn: 2.169 ± 0.535
1.252GlnPro: 1.252 ± 0.361
3.087GlnGln: 3.087 ± 0.572
3.004GlnArg: 3.004 ± 0.552
2.587GlnSer: 2.587 ± 0.492
1.585GlnThr: 1.585 ± 0.276
3.504GlnVal: 3.504 ± 0.497
0.501GlnTrp: 0.501 ± 0.183
2.837GlnTyr: 2.837 ± 0.413
0.0GlnXaa: 0.0 ± 0.0
Arg
4.088ArgAla: 4.088 ± 0.47
1.001ArgCys: 1.001 ± 0.382
4.923ArgAsp: 4.923 ± 0.627
2.587ArgGlu: 2.587 ± 0.625
1.585ArgPhe: 1.585 ± 0.418
4.673ArgGly: 4.673 ± 0.595
1.752ArgHis: 1.752 ± 0.423
3.338ArgIle: 3.338 ± 0.47
2.086ArgLys: 2.086 ± 0.409
5.257ArgLeu: 5.257 ± 0.818
1.836ArgMet: 1.836 ± 0.381
2.42ArgAsn: 2.42 ± 0.47
2.169ArgPro: 2.169 ± 0.395
2.753ArgGln: 2.753 ± 0.439
4.506ArgArg: 4.506 ± 0.494
4.005ArgSer: 4.005 ± 0.675
3.922ArgThr: 3.922 ± 0.673
4.088ArgVal: 4.088 ± 0.604
0.668ArgTrp: 0.668 ± 0.221
2.503ArgTyr: 2.503 ± 0.536
0.0ArgXaa: 0.0 ± 0.0
Ser
8.01SerAla: 8.01 ± 0.784
0.668SerCys: 0.668 ± 0.234
3.588SerAsp: 3.588 ± 0.551
2.169SerGlu: 2.169 ± 0.345
1.919SerPhe: 1.919 ± 0.424
5.34SerGly: 5.34 ± 0.609
0.751SerHis: 0.751 ± 0.221
3.004SerIle: 3.004 ± 0.479
2.753SerLys: 2.753 ± 0.39
5.924SerLeu: 5.924 ± 0.623
1.168SerMet: 1.168 ± 0.319
3.004SerAsn: 3.004 ± 0.609
2.503SerPro: 2.503 ± 0.508
2.253SerGln: 2.253 ± 0.372
3.171SerArg: 3.171 ± 0.556
3.171SerSer: 3.171 ± 0.328
4.839SerThr: 4.839 ± 0.754
5.507SerVal: 5.507 ± 0.66
1.085SerTrp: 1.085 ± 0.265
1.669SerTyr: 1.669 ± 0.322
0.0SerXaa: 0.0 ± 0.0
Thr
6.842ThrAla: 6.842 ± 0.616
0.834ThrCys: 0.834 ± 0.266
4.589ThrAsp: 4.589 ± 0.638
3.504ThrGlu: 3.504 ± 0.505
1.585ThrPhe: 1.585 ± 0.358
5.423ThrGly: 5.423 ± 0.772
1.919ThrHis: 1.919 ± 0.364
2.169ThrIle: 2.169 ± 0.392
2.753ThrLys: 2.753 ± 0.613
5.757ThrLeu: 5.757 ± 0.886
0.834ThrMet: 0.834 ± 0.248
2.837ThrAsn: 2.837 ± 0.455
2.503ThrPro: 2.503 ± 0.514
2.503ThrGln: 2.503 ± 0.483
3.588ThrArg: 3.588 ± 0.576
4.005ThrSer: 4.005 ± 0.744
4.005ThrThr: 4.005 ± 1.055
5.507ThrVal: 5.507 ± 0.769
1.168ThrTrp: 1.168 ± 0.324
2.753ThrTyr: 2.753 ± 0.658
0.0ThrXaa: 0.0 ± 0.0
Val
6.258ValAla: 6.258 ± 0.604
1.335ValCys: 1.335 ± 0.351
5.006ValAsp: 5.006 ± 0.741
3.087ValGlu: 3.087 ± 0.649
2.336ValPhe: 2.336 ± 0.517
4.839ValGly: 4.839 ± 0.445
1.752ValHis: 1.752 ± 0.537
3.588ValIle: 3.588 ± 0.634
2.587ValLys: 2.587 ± 0.757
7.343ValLeu: 7.343 ± 0.836
2.003ValMet: 2.003 ± 0.379
3.004ValAsn: 3.004 ± 0.519
4.339ValPro: 4.339 ± 0.675
4.506ValGln: 4.506 ± 0.841
5.423ValArg: 5.423 ± 0.891
4.756ValSer: 4.756 ± 0.654
4.673ValThr: 4.673 ± 0.666
5.257ValVal: 5.257 ± 0.803
0.918ValTrp: 0.918 ± 0.299
3.171ValTyr: 3.171 ± 0.515
0.0ValXaa: 0.0 ± 0.0
Trp
0.834TrpAla: 0.834 ± 0.236
0.167TrpCys: 0.167 ± 0.139
0.751TrpAsp: 0.751 ± 0.215
0.834TrpGlu: 0.834 ± 0.344
0.918TrpPhe: 0.918 ± 0.406
0.834TrpGly: 0.834 ± 0.244
0.334TrpHis: 0.334 ± 0.142
0.25TrpIle: 0.25 ± 0.132
0.25TrpLys: 0.25 ± 0.14
1.418TrpLeu: 1.418 ± 0.477
0.334TrpMet: 0.334 ± 0.147
0.501TrpAsn: 0.501 ± 0.173
0.334TrpPro: 0.334 ± 0.164
0.918TrpGln: 0.918 ± 0.259
0.918TrpArg: 0.918 ± 0.273
0.501TrpSer: 0.501 ± 0.207
0.668TrpThr: 0.668 ± 0.222
1.001TrpVal: 1.001 ± 0.24
0.167TrpTrp: 0.167 ± 0.106
0.918TrpTyr: 0.918 ± 0.237
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.003TyrAla: 2.003 ± 0.295
0.334TyrCys: 0.334 ± 0.173
2.503TyrAsp: 2.503 ± 0.449
1.836TyrGlu: 1.836 ± 0.4
1.001TyrPhe: 1.001 ± 0.288
3.171TyrGly: 3.171 ± 0.51
0.751TyrHis: 0.751 ± 0.254
2.336TyrIle: 2.336 ± 0.446
1.252TyrLys: 1.252 ± 0.417
3.755TyrLeu: 3.755 ± 0.827
1.418TyrMet: 1.418 ± 0.339
1.919TyrAsn: 1.919 ± 0.35
2.587TyrPro: 2.587 ± 0.412
1.669TyrGln: 1.669 ± 0.289
3.087TyrArg: 3.087 ± 0.415
3.254TyrSer: 3.254 ± 0.497
2.92TyrThr: 2.92 ± 0.655
2.503TyrVal: 2.503 ± 0.503
0.584TyrTrp: 0.584 ± 0.229
1.168TyrTyr: 1.168 ± 0.295
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 34 proteins (11986 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski