Strawberry vein banding virus
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q88441|Q88441_9VIRU Coat protein OS=Strawberry vein banding virus OX=47903 GN=ORF IV PE=3 SV=1
MM1 pKa = 7.67 GSVTQKK7 pKa = 8.87 TEE9 pKa = 4.06 RR10 pKa = 11.84 IYY12 pKa = 11.37 DD13 pKa = 3.52 EE14 pKa = 4.02 MLKK17 pKa = 9.47 MEE19 pKa = 4.36 EE20 pKa = 3.9 RR21 pKa = 11.84 FSRR24 pKa = 11.84 LEE26 pKa = 3.93 QEE28 pKa = 4.46 QQDD31 pKa = 4.0 HH32 pKa = 6.3 FSEE35 pKa = 5.62 KK36 pKa = 9.3 IDD38 pKa = 3.51 QASAILLAEE47 pKa = 3.79 IRR49 pKa = 11.84 KK50 pKa = 8.8 IQSKK54 pKa = 11.17 LEE56 pKa = 3.94 TCDD59 pKa = 3.53 CNKK62 pKa = 10.55 EE63 pKa = 3.83 ILDD66 pKa = 3.79 ALKK69 pKa = 10.89 AQDD72 pKa = 3.21 RR73 pKa = 11.84 TKK75 pKa = 11.18 NKK77 pKa = 10.31 GSGPSNLDD85 pKa = 3.53 ALDD88 pKa = 4.51 LAHH91 pKa = 7.49 KK92 pKa = 10.58 DD93 pKa = 3.49 NSPKK97 pKa = 10.33 RR98 pKa = 11.84 NPIRR102 pKa = 11.84 GTEE105 pKa = 3.71 NWHH108 pKa = 5.77 PQDD111 pKa = 5.06 LKK113 pKa = 9.48 LTKK116 pKa = 8.98 WW117 pKa = 3.24
Molecular weight: 13.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.113
IPC2_protein 5.995
IPC_protein 6.084
Toseland 6.389
ProMoST 6.376
Dawson 6.364
Bjellqvist 6.3
Wikipedia 6.376
Rodwell 6.351
Grimsley 6.504
Solomon 6.364
Lehninger 6.364
Nozaki 6.59
DTASelect 6.795
Thurlkill 6.795
EMBOSS 6.795
Sillero 6.722
Patrickios 4.851
IPC_peptide 6.389
IPC2_peptide 6.576
IPC2.peptide.svr19 6.661
Protein with the highest isoelectric point:
>tr|Q88443|Q88443_9VIRU ORF VI protein OS=Strawberry vein banding virus OX=47903 GN=ORF VI PE=4 SV=1
MM1 pKa = 7.66 EE2 pKa = 5.88 NNLQLLEE9 pKa = 4.22 EE10 pKa = 4.68 LNHH13 pKa = 7.69 LEE15 pKa = 4.73 EE16 pKa = 4.64 GWEE19 pKa = 3.95 MLTDD23 pKa = 3.8 EE24 pKa = 5.47 DD25 pKa = 6.11 FKK27 pKa = 11.43 LQQNEE32 pKa = 4.33 DD33 pKa = 3.81 EE34 pKa = 4.43 EE35 pKa = 4.46 QLLEE39 pKa = 4.29 SVDD42 pKa = 3.62 SEE44 pKa = 4.44 EE45 pKa = 5.18 RR46 pKa = 11.84 FSTLTKK52 pKa = 10.01 TNPNSIYY59 pKa = 10.23 IRR61 pKa = 11.84 GNFYY65 pKa = 10.26 FKK67 pKa = 10.67 GYY69 pKa = 10.34 KK70 pKa = 9.7 KK71 pKa = 10.74 YY72 pKa = 11.06 SLDD75 pKa = 3.79 LYY77 pKa = 10.59 VDD79 pKa = 4.32 TGASMCTANKK89 pKa = 10.02 HH90 pKa = 5.49 VIPEE94 pKa = 4.23 EE95 pKa = 3.76 FWVNAKK101 pKa = 10.44 NPIRR105 pKa = 11.84 ARR107 pKa = 11.84 IANDD111 pKa = 3.53 SIMTFNKK118 pKa = 9.43 VAEE121 pKa = 4.13 LMQVQIADD129 pKa = 3.66 EE130 pKa = 4.3 TFIIPTLYY138 pKa = 10.45 QATTKK143 pKa = 11.15 GDD145 pKa = 3.12 ITLGNNFCRR154 pKa = 11.84 LYY156 pKa = 10.98 EE157 pKa = 4.01 PFVQYY162 pKa = 11.36 KK163 pKa = 11.03 DD164 pKa = 3.49 MITFHH169 pKa = 6.96 KK170 pKa = 10.33 DD171 pKa = 2.48 GRR173 pKa = 11.84 AVSTKK178 pKa = 10.15 KK179 pKa = 8.48 VTKK182 pKa = 10.07 AYY184 pKa = 10.35 FHH186 pKa = 6.73 GLPGFLEE193 pKa = 4.35 SKK195 pKa = 10.43 KK196 pKa = 10.53 VGSSTSTPNPEE207 pKa = 4.37 NITPVTINQDD217 pKa = 3.72 NISKK221 pKa = 9.95 IFKK224 pKa = 10.13 GEE226 pKa = 4.03 EE227 pKa = 3.66 IGEE230 pKa = 4.26 SEE232 pKa = 4.41 QLFSTISAYY241 pKa = 9.53 TEE243 pKa = 4.01 VEE245 pKa = 3.97 KK246 pKa = 11.1 LLDD249 pKa = 4.57 SICSEE254 pKa = 4.35 HH255 pKa = 7.0 PLDD258 pKa = 4.66 SRR260 pKa = 11.84 INKK263 pKa = 9.53 GKK265 pKa = 10.7 FEE267 pKa = 4.02 AQIALLDD274 pKa = 3.61 TNKK277 pKa = 10.11 IIKK280 pKa = 8.56 CKK282 pKa = 9.69 PMQYY286 pKa = 10.22 SPQDD290 pKa = 3.24 RR291 pKa = 11.84 EE292 pKa = 3.95 EE293 pKa = 4.08 FKK295 pKa = 10.25 TQIEE299 pKa = 4.31 EE300 pKa = 4.11 LLKK303 pKa = 10.97 LGIIRR308 pKa = 11.84 PSKK311 pKa = 10.44 SPHH314 pKa = 6.05 SSPAFMVRR322 pKa = 11.84 NHH324 pKa = 6.76 AEE326 pKa = 3.83 IKK328 pKa = 9.7 RR329 pKa = 11.84 GKK331 pKa = 10.15 ARR333 pKa = 11.84 MVINYY338 pKa = 8.06 KK339 pKa = 10.32 KK340 pKa = 10.96 LNDD343 pKa = 3.53 HH344 pKa = 6.52 TKK346 pKa = 10.7 GDD348 pKa = 4.18 GYY350 pKa = 11.37 LLPNKK355 pKa = 9.54 EE356 pKa = 4.03 QLLQRR361 pKa = 11.84 IGGKK365 pKa = 7.24 TFYY368 pKa = 11.14 SSFDD372 pKa = 3.75 CKK374 pKa = 10.91 SGFWQVRR381 pKa = 11.84 LAPEE385 pKa = 5.06 TIQLTAFSCPQGHH398 pKa = 5.69 YY399 pKa = 9.03 EE400 pKa = 3.94 WLVMPFGLKK409 pKa = 9.04 QAPAIFQRR417 pKa = 11.84 HH418 pKa = 4.77 MDD420 pKa = 3.46 EE421 pKa = 4.83 SLSNMYY427 pKa = 9.71 PQFCAVYY434 pKa = 9.19 VDD436 pKa = 4.82 DD437 pKa = 6.12 IIVFSKK443 pKa = 10.0 TEE445 pKa = 4.01 EE446 pKa = 3.83 EE447 pKa = 4.28 HH448 pKa = 6.5 LGHH451 pKa = 6.61 VKK453 pKa = 10.05 IVLNRR458 pKa = 11.84 CKK460 pKa = 10.63 ALGIVLSKK468 pKa = 10.87 KK469 pKa = 9.87 KK470 pKa = 10.54 AQLCKK475 pKa = 8.61 TTINFLGLVIEE486 pKa = 4.63 RR487 pKa = 11.84 GNLKK491 pKa = 9.61 VQSHH495 pKa = 6.75 IGLHH499 pKa = 5.94 LVAFPDD505 pKa = 3.61 QLSDD509 pKa = 3.36 RR510 pKa = 11.84 NALQRR515 pKa = 11.84 FLGLLNYY522 pKa = 9.96 ISAYY526 pKa = 9.1 FPKK529 pKa = 10.03 IANLRR534 pKa = 11.84 SPLQVKK540 pKa = 9.46 LKK542 pKa = 11.05 KK543 pKa = 10.31 EE544 pKa = 4.27 ITWSWTEE551 pKa = 3.54 KK552 pKa = 9.89 DD553 pKa = 3.3 TEE555 pKa = 4.38 TVRR558 pKa = 11.84 KK559 pKa = 9.39 IKK561 pKa = 10.77 SLVKK565 pKa = 9.74 TLPDD569 pKa = 4.37 LYY571 pKa = 11.08 NPSPEE576 pKa = 4.2 DD577 pKa = 3.66 KK578 pKa = 10.84 PIIEE582 pKa = 5.18 CDD584 pKa = 3.38 ASDD587 pKa = 3.98 DD588 pKa = 3.4 HH589 pKa = 6.84 WGAILKK595 pKa = 10.54 AKK597 pKa = 10.21 LPEE600 pKa = 4.37 GKK602 pKa = 9.94 EE603 pKa = 4.14 VICRR607 pKa = 11.84 YY608 pKa = 10.34 ASGTFKK614 pKa = 10.46 PAEE617 pKa = 4.16 KK618 pKa = 10.04 NYY620 pKa = 10.23 HH621 pKa = 5.5 SNEE624 pKa = 3.89 KK625 pKa = 10.26 EE626 pKa = 3.54 ILSIIKK632 pKa = 9.95 AIKK635 pKa = 9.99 AFRR638 pKa = 11.84 AYY640 pKa = 9.61 ILPYY644 pKa = 9.78 KK645 pKa = 10.45 FLVRR649 pKa = 11.84 TDD651 pKa = 3.64 NTNAAYY657 pKa = 9.54 FVRR660 pKa = 11.84 TNIAGDD666 pKa = 3.7 YY667 pKa = 8.76 KK668 pKa = 10.47 QSRR671 pKa = 11.84 LVRR674 pKa = 11.84 WQMALRR680 pKa = 11.84 EE681 pKa = 3.91 YY682 pKa = 10.99 SFDD685 pKa = 3.45 IEE687 pKa = 4.39 HH688 pKa = 7.15 VSGQKK693 pKa = 10.16 NVLADD698 pKa = 3.1 IMTRR702 pKa = 11.84 EE703 pKa = 4.15 LAGKK707 pKa = 7.03 TT708 pKa = 3.48
Molecular weight: 81.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.139
IPC2_protein 7.6
IPC_protein 7.439
Toseland 7.249
ProMoST 8.009
Dawson 8.214
Bjellqvist 8.463
Wikipedia 8.141
Rodwell 8.258
Grimsley 7.322
Solomon 8.273
Lehninger 8.273
Nozaki 8.639
DTASelect 8.258
Thurlkill 8.346
EMBOSS 8.419
Sillero 8.624
Patrickios 4.457
IPC_peptide 8.258
IPC2_peptide 7.293
IPC2.peptide.svr19 7.618
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2302
117
708
383.7
44.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.778 ± 0.806
1.781 ± 0.313
5.083 ± 0.25
9.296 ± 0.793
3.475 ± 0.497
4.083 ± 0.453
2.433 ± 0.284
7.081 ± 0.457
9.6 ± 0.516
9.513 ± 0.267
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.738 ± 0.282
5.039 ± 0.461
4.344 ± 0.313
4.561 ± 0.669
5.213 ± 0.476
7.515 ± 0.367
5.908 ± 0.117
3.736 ± 0.449
0.956 ± 0.119
3.866 ± 0.338
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here