Synechococcus phage S-WAM1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Libanvirus; unclassified Libanvirus

Average proteome isoelectric point is 5.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 221 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D8KSQ4|A0A1D8KSQ4_9CAUD Peptidase OS=Synechococcus phage S-WAM1 OX=1815521 GN=P090810_134 PE=4 SV=1
MM1 pKa = 7.82ADD3 pKa = 3.4ASFLYY8 pKa = 10.16IAFFLTIGSFVLGFISSWNLKK29 pKa = 9.75HH30 pKa = 7.03VFDD33 pKa = 4.11VWVDD37 pKa = 3.2RR38 pKa = 11.84ADD40 pKa = 3.52YY41 pKa = 10.81ARR43 pKa = 11.84VVMHH47 pKa = 6.84PEE49 pKa = 3.94MYY51 pKa = 10.6DD52 pKa = 3.45EE53 pKa = 6.46DD54 pKa = 5.67GMLTDD59 pKa = 3.82QPLIYY64 pKa = 10.46LHH66 pKa = 7.77IEE68 pKa = 4.43DD69 pKa = 5.23EE70 pKa = 4.54DD71 pKa = 4.81DD72 pKa = 3.42MMYY75 pKa = 11.07DD76 pKa = 3.76EE77 pKa = 6.61DD78 pKa = 4.97DD79 pKa = 3.44

Molecular weight: 9.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D8KS44|A0A1D8KS44_9CAUD Uncharacterized protein OS=Synechococcus phage S-WAM1 OX=1815521 GN=P090810_030 PE=4 SV=1
MM1 pKa = 7.06NTPNWQHH8 pKa = 6.03HH9 pKa = 5.47SKK11 pKa = 10.91KK12 pKa = 9.31EE13 pKa = 3.94AKK15 pKa = 8.62RR16 pKa = 11.84TLKK19 pKa = 10.42PQALRR24 pKa = 11.84QAKK27 pKa = 9.23KK28 pKa = 10.21RR29 pKa = 11.84RR30 pKa = 11.84AALKK34 pKa = 10.43AKK36 pKa = 10.12LLAASVVLVGAASPAQAVTWGEE58 pKa = 3.56FWEE61 pKa = 4.78PFTGDD66 pKa = 2.98RR67 pKa = 11.84HH68 pKa = 5.17HH69 pKa = 6.77HH70 pKa = 5.3HH71 pKa = 6.72VEE73 pKa = 3.18RR74 pKa = 11.84HH75 pKa = 5.25YY76 pKa = 10.5YY77 pKa = 9.68HH78 pKa = 7.57PAPPPPRR85 pKa = 11.84MCRR88 pKa = 11.84RR89 pKa = 11.84KK90 pKa = 9.81QVVDD94 pKa = 2.91TWVEE98 pKa = 3.78PTPFYY103 pKa = 10.34PGYY106 pKa = 7.78WSQRR110 pKa = 11.84IRR112 pKa = 11.84WTWEE116 pKa = 3.13PCHH119 pKa = 6.13RR120 pKa = 11.84HH121 pKa = 3.95RR122 pKa = 11.84HH123 pKa = 5.59RR124 pKa = 11.84YY125 pKa = 8.66DD126 pKa = 2.93HH127 pKa = 7.04

Molecular weight: 15.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

221

0

221

57904

35

6737

262.0

29.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.088 ± 0.206

0.92 ± 0.093

6.92 ± 0.173

6.131 ± 0.263

4.302 ± 0.111

7.939 ± 0.35

1.743 ± 0.149

6.155 ± 0.28

5.468 ± 0.407

7.103 ± 0.214

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.083 ± 0.187

5.915 ± 0.224

4.076 ± 0.137

3.791 ± 0.088

4.312 ± 0.165

6.702 ± 0.159

7.265 ± 0.409

6.588 ± 0.163

1.314 ± 0.095

4.185 ± 0.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski