Amino acid dipepetide frequency for Synechococcus phage S-WAM1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.787AlaAla: 6.787 ± 0.514
0.397AlaCys: 0.397 ± 0.1
4.628AlaAsp: 4.628 ± 0.355
3.955AlaGlu: 3.955 ± 0.297
2.66AlaPhe: 2.66 ± 0.225
6.701AlaGly: 6.701 ± 0.428
1.088AlaHis: 1.088 ± 0.162
3.644AlaIle: 3.644 ± 0.23
3.903AlaLys: 3.903 ± 0.497
4.905AlaLeu: 4.905 ± 0.325
1.451AlaMet: 1.451 ± 0.197
4.473AlaAsn: 4.473 ± 0.4
2.746AlaPro: 2.746 ± 0.196
2.66AlaGln: 2.66 ± 0.213
2.988AlaArg: 2.988 ± 0.234
5.147AlaSer: 5.147 ± 0.377
5.526AlaThr: 5.526 ± 0.548
3.972AlaVal: 3.972 ± 0.29
0.829AlaTrp: 0.829 ± 0.126
2.418AlaTyr: 2.418 ± 0.193
0.0AlaXaa: 0.0 ± 0.0
Cys
0.57CysAla: 0.57 ± 0.095
0.104CysCys: 0.104 ± 0.039
0.812CysAsp: 0.812 ± 0.118
0.535CysGlu: 0.535 ± 0.11
0.397CysPhe: 0.397 ± 0.098
0.553CysGly: 0.553 ± 0.099
0.363CysHis: 0.363 ± 0.107
0.604CysIle: 0.604 ± 0.111
0.57CysLys: 0.57 ± 0.111
0.414CysLeu: 0.414 ± 0.092
0.242CysMet: 0.242 ± 0.079
0.553CysAsn: 0.553 ± 0.117
0.449CysPro: 0.449 ± 0.099
0.294CysGln: 0.294 ± 0.066
0.397CysArg: 0.397 ± 0.092
0.656CysSer: 0.656 ± 0.111
0.432CysThr: 0.432 ± 0.097
0.639CysVal: 0.639 ± 0.121
0.173CysTrp: 0.173 ± 0.058
0.449CysTyr: 0.449 ± 0.13
0.0CysXaa: 0.0 ± 0.0
Asp
5.129AspAla: 5.129 ± 0.321
0.639AspCys: 0.639 ± 0.131
4.991AspAsp: 4.991 ± 0.348
4.335AspGlu: 4.335 ± 0.349
3.281AspPhe: 3.281 ± 0.214
5.699AspGly: 5.699 ± 0.447
1.053AspHis: 1.053 ± 0.188
4.68AspIle: 4.68 ± 0.482
3.143AspLys: 3.143 ± 0.331
5.457AspLeu: 5.457 ± 0.331
1.589AspMet: 1.589 ± 0.166
3.696AspAsn: 3.696 ± 0.309
3.109AspPro: 3.109 ± 0.272
2.435AspGln: 2.435 ± 0.217
2.781AspArg: 2.781 ± 0.203
4.093AspSer: 4.093 ± 0.299
4.352AspThr: 4.352 ± 0.315
4.853AspVal: 4.853 ± 0.326
1.071AspTrp: 1.071 ± 0.174
2.815AspTyr: 2.815 ± 0.221
0.0AspXaa: 0.0 ± 0.0
Glu
3.817GluAla: 3.817 ± 0.32
0.691GluCys: 0.691 ± 0.136
4.214GluAsp: 4.214 ± 0.286
4.438GluGlu: 4.438 ± 0.481
3.16GluPhe: 3.16 ± 0.248
4.11GluGly: 4.11 ± 0.265
1.278GluHis: 1.278 ± 0.205
3.886GluIle: 3.886 ± 0.324
3.558GluLys: 3.558 ± 0.464
4.991GluLeu: 4.991 ± 0.419
1.623GluMet: 1.623 ± 0.23
2.832GluAsn: 2.832 ± 0.242
2.124GluPro: 2.124 ± 0.207
2.262GluGln: 2.262 ± 0.267
2.711GluArg: 2.711 ± 0.234
3.609GluSer: 3.609 ± 0.278
3.92GluThr: 3.92 ± 0.253
4.318GluVal: 4.318 ± 0.267
0.846GluTrp: 0.846 ± 0.16
2.919GluTyr: 2.919 ± 0.247
0.0GluXaa: 0.0 ± 0.0
Phe
2.591PheAla: 2.591 ± 0.226
0.38PheCys: 0.38 ± 0.093
3.575PheAsp: 3.575 ± 0.286
2.85PheGlu: 2.85 ± 0.194
1.727PhePhe: 1.727 ± 0.154
3.143PheGly: 3.143 ± 0.267
0.76PheHis: 0.76 ± 0.155
2.487PheIle: 2.487 ± 0.206
2.228PheLys: 2.228 ± 0.261
2.763PheLeu: 2.763 ± 0.239
0.881PheMet: 0.881 ± 0.171
3.04PheAsn: 3.04 ± 0.251
1.917PhePro: 1.917 ± 0.214
1.71PheGln: 1.71 ± 0.165
1.503PheArg: 1.503 ± 0.182
3.091PheSer: 3.091 ± 0.207
3.661PheThr: 3.661 ± 0.271
2.729PheVal: 2.729 ± 0.215
0.414PheTrp: 0.414 ± 0.099
1.572PheTyr: 1.572 ± 0.169
0.0PheXaa: 0.0 ± 0.0
Gly
6.165GlyAla: 6.165 ± 0.538
0.639GlyCys: 0.639 ± 0.172
5.233GlyAsp: 5.233 ± 0.321
4.473GlyGlu: 4.473 ± 0.299
3.057GlyPhe: 3.057 ± 0.272
8.393GlyGly: 8.393 ± 1.011
1.226GlyHis: 1.226 ± 0.158
4.076GlyIle: 4.076 ± 0.25
4.024GlyLys: 4.024 ± 0.414
4.905GlyLeu: 4.905 ± 0.365
1.503GlyMet: 1.503 ± 0.247
4.991GlyAsn: 4.991 ± 0.685
2.072GlyPro: 2.072 ± 0.241
2.85GlyGln: 2.85 ± 0.199
3.558GlyArg: 3.558 ± 0.225
6.528GlySer: 6.528 ± 0.506
6.528GlyThr: 6.528 ± 0.817
4.508GlyVal: 4.508 ± 0.278
1.313GlyTrp: 1.313 ± 0.148
3.35GlyTyr: 3.35 ± 0.349
0.0GlyXaa: 0.0 ± 0.0
His
0.933HisAla: 0.933 ± 0.126
0.19HisCys: 0.19 ± 0.055
0.95HisAsp: 0.95 ± 0.134
1.174HisGlu: 1.174 ± 0.185
0.864HisPhe: 0.864 ± 0.16
1.554HisGly: 1.554 ± 0.209
0.587HisHis: 0.587 ± 0.109
0.933HisIle: 0.933 ± 0.139
1.088HisLys: 1.088 ± 0.203
0.933HisLeu: 0.933 ± 0.113
0.535HisMet: 0.535 ± 0.13
0.864HisAsn: 0.864 ± 0.161
1.036HisPro: 1.036 ± 0.15
0.743HisGln: 0.743 ± 0.118
0.76HisArg: 0.76 ± 0.145
1.002HisSer: 1.002 ± 0.138
1.053HisThr: 1.053 ± 0.137
1.036HisVal: 1.036 ± 0.158
0.345HisTrp: 0.345 ± 0.09
0.846HisTyr: 0.846 ± 0.156
0.0HisXaa: 0.0 ± 0.0
Ile
4.266IleAla: 4.266 ± 0.372
0.501IleCys: 0.501 ± 0.107
5.026IleAsp: 5.026 ± 0.407
4.266IleGlu: 4.266 ± 0.307
1.969IlePhe: 1.969 ± 0.169
4.145IleGly: 4.145 ± 0.28
1.036IleHis: 1.036 ± 0.125
3.627IleIle: 3.627 ± 0.329
3.799IleLys: 3.799 ± 0.302
4.3IleLeu: 4.3 ± 0.353
0.898IleMet: 0.898 ± 0.12
3.92IleAsn: 3.92 ± 0.35
2.85IlePro: 2.85 ± 0.227
2.521IleGln: 2.521 ± 0.197
2.314IleArg: 2.314 ± 0.206
4.559IleSer: 4.559 ± 0.516
4.974IleThr: 4.974 ± 0.652
3.558IleVal: 3.558 ± 0.309
0.691IleTrp: 0.691 ± 0.108
2.331IleTyr: 2.331 ± 0.245
0.0IleXaa: 0.0 ± 0.0
Lys
3.212LysAla: 3.212 ± 0.403
0.414LysCys: 0.414 ± 0.087
3.368LysAsp: 3.368 ± 0.352
3.869LysGlu: 3.869 ± 0.443
2.418LysPhe: 2.418 ± 0.264
3.247LysGly: 3.247 ± 0.342
1.019LysHis: 1.019 ± 0.207
4.594LysIle: 4.594 ± 0.316
4.248LysLys: 4.248 ± 0.619
3.799LysLeu: 3.799 ± 0.382
1.451LysMet: 1.451 ± 0.224
3.022LysAsn: 3.022 ± 0.347
1.865LysPro: 1.865 ± 0.243
2.176LysGln: 2.176 ± 0.289
2.504LysArg: 2.504 ± 0.323
3.661LysSer: 3.661 ± 0.38
2.97LysThr: 2.97 ± 0.196
3.368LysVal: 3.368 ± 0.327
0.622LysTrp: 0.622 ± 0.121
2.85LysTyr: 2.85 ± 0.339
0.0LysXaa: 0.0 ± 0.0
Leu
4.801LeuAla: 4.801 ± 0.266
0.743LeuCys: 0.743 ± 0.154
5.889LeuAsp: 5.889 ± 0.366
4.508LeuGlu: 4.508 ± 0.5
2.936LeuPhe: 2.936 ± 0.228
4.732LeuGly: 4.732 ± 0.405
1.261LeuHis: 1.261 ± 0.202
4.369LeuIle: 4.369 ± 0.279
3.817LeuLys: 3.817 ± 0.36
4.887LeuLeu: 4.887 ± 0.363
1.382LeuMet: 1.382 ± 0.228
4.87LeuAsn: 4.87 ± 0.313
3.005LeuPro: 3.005 ± 0.247
2.781LeuGln: 2.781 ± 0.248
3.42LeuArg: 3.42 ± 0.286
4.542LeuSer: 4.542 ± 0.254
5.216LeuThr: 5.216 ± 0.592
4.3LeuVal: 4.3 ± 0.244
0.604LeuTrp: 0.604 ± 0.15
2.97LeuTyr: 2.97 ± 0.238
0.0LeuXaa: 0.0 ± 0.0
Met
1.658MetAla: 1.658 ± 0.228
0.207MetCys: 0.207 ± 0.065
0.846MetAsp: 0.846 ± 0.206
1.105MetGlu: 1.105 ± 0.17
0.846MetPhe: 0.846 ± 0.121
1.105MetGly: 1.105 ± 0.185
0.484MetHis: 0.484 ± 0.111
1.261MetIle: 1.261 ± 0.163
1.762MetLys: 1.762 ± 0.296
1.606MetLeu: 1.606 ± 0.197
0.466MetMet: 0.466 ± 0.103
1.209MetAsn: 1.209 ± 0.157
0.915MetPro: 0.915 ± 0.157
1.019MetGln: 1.019 ± 0.173
0.95MetArg: 0.95 ± 0.122
1.727MetSer: 1.727 ± 0.231
1.485MetThr: 1.485 ± 0.241
1.14MetVal: 1.14 ± 0.162
0.345MetTrp: 0.345 ± 0.069
0.691MetTyr: 0.691 ± 0.119
0.0MetXaa: 0.0 ± 0.0
Asn
3.869AsnAla: 3.869 ± 0.442
0.846AsnCys: 0.846 ± 0.128
3.195AsnAsp: 3.195 ± 0.229
3.54AsnGlu: 3.54 ± 0.245
2.763AsnPhe: 2.763 ± 0.204
4.646AsnGly: 4.646 ± 0.381
0.846AsnHis: 0.846 ± 0.121
3.938AsnIle: 3.938 ± 0.402
2.746AsnLys: 2.746 ± 0.207
4.698AsnLeu: 4.698 ± 0.376
1.036AsnMet: 1.036 ± 0.161
3.489AsnAsn: 3.489 ± 0.404
3.54AsnPro: 3.54 ± 0.24
2.193AsnGln: 2.193 ± 0.232
2.729AsnArg: 2.729 ± 0.187
3.471AsnSer: 3.471 ± 0.324
4.145AsnThr: 4.145 ± 0.529
4.162AsnVal: 4.162 ± 0.366
0.622AsnTrp: 0.622 ± 0.108
2.677AsnTyr: 2.677 ± 0.207
0.0AsnXaa: 0.0 ± 0.0
Pro
2.711ProAla: 2.711 ± 0.243
0.328ProCys: 0.328 ± 0.088
2.97ProAsp: 2.97 ± 0.331
2.815ProGlu: 2.815 ± 0.266
1.779ProPhe: 1.779 ± 0.249
3.471ProGly: 3.471 ± 0.327
0.777ProHis: 0.777 ± 0.132
2.124ProIle: 2.124 ± 0.2
2.193ProLys: 2.193 ± 0.279
2.591ProLeu: 2.591 ± 0.225
0.587ProMet: 0.587 ± 0.145
2.142ProAsn: 2.142 ± 0.169
1.762ProPro: 1.762 ± 0.2
1.503ProGln: 1.503 ± 0.166
1.71ProArg: 1.71 ± 0.239
2.919ProSer: 2.919 ± 0.25
3.195ProThr: 3.195 ± 0.286
2.832ProVal: 2.832 ± 0.234
0.604ProTrp: 0.604 ± 0.082
1.744ProTyr: 1.744 ± 0.2
0.0ProXaa: 0.0 ± 0.0
Gln
2.418GlnAla: 2.418 ± 0.185
0.466GlnCys: 0.466 ± 0.101
2.124GlnAsp: 2.124 ± 0.193
2.349GlnGlu: 2.349 ± 0.223
1.969GlnPhe: 1.969 ± 0.15
2.66GlnGly: 2.66 ± 0.319
0.535GlnHis: 0.535 ± 0.108
2.401GlnIle: 2.401 ± 0.211
2.003GlnLys: 2.003 ± 0.248
2.832GlnLeu: 2.832 ± 0.21
1.209GlnMet: 1.209 ± 0.186
2.021GlnAsn: 2.021 ± 0.177
1.641GlnPro: 1.641 ± 0.145
1.762GlnGln: 1.762 ± 0.247
1.658GlnArg: 1.658 ± 0.183
2.401GlnSer: 2.401 ± 0.229
2.228GlnThr: 2.228 ± 0.18
2.556GlnVal: 2.556 ± 0.207
0.777GlnTrp: 0.777 ± 0.097
1.9GlnTyr: 1.9 ± 0.206
0.0GlnXaa: 0.0 ± 0.0
Arg
2.798ArgAla: 2.798 ± 0.207
0.484ArgCys: 0.484 ± 0.112
2.85ArgAsp: 2.85 ± 0.26
2.193ArgGlu: 2.193 ± 0.29
2.159ArgPhe: 2.159 ± 0.172
3.23ArgGly: 3.23 ± 0.214
1.019ArgHis: 1.019 ± 0.174
2.953ArgIle: 2.953 ± 0.213
2.591ArgLys: 2.591 ± 0.284
3.23ArgLeu: 3.23 ± 0.203
1.157ArgMet: 1.157 ± 0.184
2.142ArgAsn: 2.142 ± 0.197
1.52ArgPro: 1.52 ± 0.145
1.658ArgGln: 1.658 ± 0.183
2.055ArgArg: 2.055 ± 0.256
2.694ArgSer: 2.694 ± 0.252
2.573ArgThr: 2.573 ± 0.28
3.091ArgVal: 3.091 ± 0.275
0.743ArgTrp: 0.743 ± 0.12
1.986ArgTyr: 1.986 ± 0.193
0.0ArgXaa: 0.0 ± 0.0
Ser
5.25SerAla: 5.25 ± 0.286
0.328SerCys: 0.328 ± 0.077
4.231SerAsp: 4.231 ± 0.292
3.851SerGlu: 3.851 ± 0.283
3.16SerPhe: 3.16 ± 0.193
6.891SerGly: 6.891 ± 0.484
0.95SerHis: 0.95 ± 0.135
4.335SerIle: 4.335 ± 0.345
3.437SerLys: 3.437 ± 0.318
4.266SerLeu: 4.266 ± 0.24
1.399SerMet: 1.399 ± 0.2
4.179SerAsn: 4.179 ± 0.278
2.435SerPro: 2.435 ± 0.229
2.211SerGln: 2.211 ± 0.191
2.401SerArg: 2.401 ± 0.19
4.853SerSer: 4.853 ± 0.342
5.233SerThr: 5.233 ± 0.547
4.335SerVal: 4.335 ± 0.299
0.708SerTrp: 0.708 ± 0.115
2.556SerTyr: 2.556 ± 0.217
0.0SerXaa: 0.0 ± 0.0
Thr
5.613ThrAla: 5.613 ± 0.464
0.553ThrCys: 0.553 ± 0.099
4.577ThrAsp: 4.577 ± 0.548
3.592ThrGlu: 3.592 ± 0.274
2.988ThrPhe: 2.988 ± 0.265
6.925ThrGly: 6.925 ± 0.803
1.002ThrHis: 1.002 ± 0.127
4.559ThrIle: 4.559 ± 0.477
3.35ThrLys: 3.35 ± 0.337
5.699ThrLeu: 5.699 ± 0.719
1.036ThrMet: 1.036 ± 0.142
4.162ThrAsn: 4.162 ± 0.447
3.42ThrPro: 3.42 ± 0.286
2.694ThrGln: 2.694 ± 0.198
2.763ThrArg: 2.763 ± 0.251
4.231ThrSer: 4.231 ± 0.471
5.699ThrThr: 5.699 ± 0.711
6.114ThrVal: 6.114 ± 0.646
0.95ThrTrp: 0.95 ± 0.131
2.729ThrTyr: 2.729 ± 0.227
0.0ThrXaa: 0.0 ± 0.0
Val
4.887ValAla: 4.887 ± 0.354
0.501ValCys: 0.501 ± 0.103
5.233ValAsp: 5.233 ± 0.499
3.955ValGlu: 3.955 ± 0.284
2.711ValPhe: 2.711 ± 0.222
4.611ValGly: 4.611 ± 0.319
1.071ValHis: 1.071 ± 0.126
4.076ValIle: 4.076 ± 0.501
3.454ValLys: 3.454 ± 0.268
4.663ValLeu: 4.663 ± 0.262
1.313ValMet: 1.313 ± 0.156
3.869ValAsn: 3.869 ± 0.384
2.297ValPro: 2.297 ± 0.285
2.262ValGln: 2.262 ± 0.24
2.97ValArg: 2.97 ± 0.257
4.3ValSer: 4.3 ± 0.267
5.906ValThr: 5.906 ± 0.562
4.508ValVal: 4.508 ± 0.428
0.656ValTrp: 0.656 ± 0.083
2.642ValTyr: 2.642 ± 0.203
0.0ValXaa: 0.0 ± 0.0
Trp
0.691TrpAla: 0.691 ± 0.139
0.242TrpCys: 0.242 ± 0.074
1.053TrpAsp: 1.053 ± 0.179
0.864TrpGlu: 0.864 ± 0.159
0.501TrpPhe: 0.501 ± 0.09
0.76TrpGly: 0.76 ± 0.151
0.432TrpHis: 0.432 ± 0.086
0.76TrpIle: 0.76 ± 0.124
0.691TrpLys: 0.691 ± 0.132
0.829TrpLeu: 0.829 ± 0.119
0.328TrpMet: 0.328 ± 0.094
1.002TrpAsn: 1.002 ± 0.132
0.345TrpPro: 0.345 ± 0.08
0.466TrpGln: 0.466 ± 0.095
0.57TrpArg: 0.57 ± 0.117
0.812TrpSer: 0.812 ± 0.103
0.777TrpThr: 0.777 ± 0.109
0.898TrpVal: 0.898 ± 0.139
0.311TrpTrp: 0.311 ± 0.079
0.812TrpTyr: 0.812 ± 0.14
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.711TyrAla: 2.711 ± 0.2
0.553TyrCys: 0.553 ± 0.109
3.437TyrAsp: 3.437 ± 0.277
2.642TyrGlu: 2.642 ± 0.168
1.675TyrPhe: 1.675 ± 0.154
2.815TyrGly: 2.815 ± 0.25
0.639TyrHis: 0.639 ± 0.124
2.245TyrIle: 2.245 ± 0.237
2.072TyrLys: 2.072 ± 0.189
3.264TyrLeu: 3.264 ± 0.29
0.725TyrMet: 0.725 ± 0.13
2.677TyrAsn: 2.677 ± 0.218
1.71TyrPro: 1.71 ± 0.211
1.71TyrGln: 1.71 ± 0.199
2.383TyrArg: 2.383 ± 0.234
2.729TyrSer: 2.729 ± 0.242
2.781TyrThr: 2.781 ± 0.213
2.97TyrVal: 2.97 ± 0.229
0.518TyrTrp: 0.518 ± 0.091
1.589TyrTyr: 1.589 ± 0.186
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 221 proteins (57904 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski