Edwardsiella phage MSW-3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Yokohamavirus; Edwardsiella virus MSW3

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L0MZ26|L0MZ26_9CAUD Uncharacterized protein OS=Edwardsiella phage MSW-3 OX=1264700 PE=4 SV=1
MM1 pKa = 8.44IEE3 pKa = 4.1IPLKK7 pKa = 10.65GGAANAHH14 pKa = 4.31QTFNIQLGDD23 pKa = 3.75TYY25 pKa = 11.81VDD27 pKa = 3.93FTLNYY32 pKa = 10.11VSYY35 pKa = 9.49TDD37 pKa = 3.6KK38 pKa = 10.63PAWSMDD44 pKa = 2.64ISRR47 pKa = 11.84DD48 pKa = 3.34GAPLVNGAMLEE59 pKa = 4.19PGCDD63 pKa = 3.41VIQSYY68 pKa = 9.59GAGIGKK74 pKa = 9.87LIFIGAEE81 pKa = 3.86VTLDD85 pKa = 3.55NLGTDD90 pKa = 3.25NHH92 pKa = 6.35LVWVSEE98 pKa = 4.08

Molecular weight: 10.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L0MZ40|L0MZ40_9CAUD Uncharacterized protein OS=Edwardsiella phage MSW-3 OX=1264700 PE=4 SV=1
MM1 pKa = 7.12TRR3 pKa = 11.84AKK5 pKa = 10.39SRR7 pKa = 11.84EE8 pKa = 3.83KK9 pKa = 10.25LTVGVRR15 pKa = 11.84NNKK18 pKa = 8.24VRR20 pKa = 11.84NSTDD24 pKa = 2.86GMVHH28 pKa = 6.32SFIFPFTANQNLMSAGGTRR47 pKa = 11.84CEE49 pKa = 3.97

Molecular weight: 5.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

12662

31

782

191.8

21.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.872 ± 0.558

1.406 ± 0.138

6.531 ± 0.258

5.457 ± 0.23

3.467 ± 0.186

7.7 ± 0.308

1.864 ± 0.182

5.189 ± 0.189

4.754 ± 0.181

7.195 ± 0.285

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.97 ± 0.145

4.454 ± 0.237

4.415 ± 0.298

3.609 ± 0.214

6.192 ± 0.271

5.994 ± 0.265

6.413 ± 0.335

7.692 ± 0.326

1.627 ± 0.148

3.199 ± 0.209
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski