Planctomyces sp. SH-PL62

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Planctomycetales; Planctomycetaceae; Planctomyces; unclassified Planctomyces

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5245 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A142YFM9|A0A142YFM9_9PLAN Ribosomal RNA small subunit methyltransferase H OS=Planctomyces sp. SH-PL62 OX=1636152 GN=rsmH_1 PE=3 SV=1
MM1 pKa = 7.71PRR3 pKa = 11.84RR4 pKa = 11.84GDD6 pKa = 3.35EE7 pKa = 4.24DD8 pKa = 5.33DD9 pKa = 5.61RR10 pKa = 11.84DD11 pKa = 4.17DD12 pKa = 5.82DD13 pKa = 4.86VDD15 pKa = 4.29DD16 pKa = 6.26ALDD19 pKa = 4.15EE20 pKa = 5.06DD21 pKa = 5.59DD22 pKa = 5.16GDD24 pKa = 4.0EE25 pKa = 4.25TAPCPYY31 pKa = 9.34CGNPIHH37 pKa = 6.84EE38 pKa = 4.43EE39 pKa = 4.12SEE41 pKa = 3.95RR42 pKa = 11.84CPYY45 pKa = 10.28CNTYY49 pKa = 10.11ISRR52 pKa = 11.84EE53 pKa = 3.89DD54 pKa = 3.15APYY57 pKa = 10.44VRR59 pKa = 11.84KK60 pKa = 8.88PWWIIVGVIAGLYY73 pKa = 9.35AVYY76 pKa = 10.11RR77 pKa = 11.84WVAFF81 pKa = 3.78

Molecular weight: 9.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A142YCE9|A0A142YCE9_9PLAN Uncharacterized protein OS=Planctomyces sp. SH-PL62 OX=1636152 GN=VT85_04830 PE=4 SV=1
MM1 pKa = 7.28GRR3 pKa = 11.84MRR5 pKa = 11.84QGGPNVPGGGLGANAGANPGGFTPRR30 pKa = 11.84MRR32 pKa = 11.84GGGASPSFAPQLHH45 pKa = 7.0ASPAPNPGVAGLGSNPEE62 pKa = 4.1GLGGMRR68 pKa = 11.84GMRR71 pKa = 11.84SSGVANAPGLAPGFGAGAVNAGGPAGFNGGAMRR104 pKa = 11.84GAGTPNFGAPPAAGAINPGFGGMRR128 pKa = 11.84GVNVGGGGNSAGGLGSGFSGGMRR151 pKa = 11.84GPGGGFGGGMRR162 pKa = 11.84GAGGFGGGVGTGGFGGTPSGGGFGGMRR189 pKa = 11.84GGGGMGGVGGSGFGGGMGGGGFGGGGGGGMGAAGGVNVGGGGGMGGAGGGGFGGHH244 pKa = 5.87MRR246 pKa = 4.17

Molecular weight: 21.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5245

0

5245

1933445

29

4434

368.6

40.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.173 ± 0.04

0.858 ± 0.011

6.16 ± 0.027

6.006 ± 0.031

3.485 ± 0.019

8.908 ± 0.037

1.941 ± 0.015

4.052 ± 0.021

2.941 ± 0.028

10.058 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.877 ± 0.015

2.238 ± 0.022

6.446 ± 0.029

2.686 ± 0.019

8.274 ± 0.039

5.517 ± 0.028

4.985 ± 0.028

7.591 ± 0.028

1.56 ± 0.015

2.243 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski