Piscine orthoreovirus
Average proteome isoelectric point is 6.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S2EGR3|A0A0S2EGR3_9REOV RNA-directed RNA polymerase (Fragment) OS=Piscine orthoreovirus OX=1157337 PE=2 SV=1
MM1 pKa = 7.02 ATLYY5 pKa = 10.73 GLRR8 pKa = 11.84 ITRR11 pKa = 11.84 LKK13 pKa = 10.65 NLPLLEE19 pKa = 5.31 RR20 pKa = 11.84 DD21 pKa = 3.68 TEE23 pKa = 4.25 EE24 pKa = 3.86 YY25 pKa = 9.35 TYY27 pKa = 11.39 KK28 pKa = 10.78 EE29 pKa = 4.23 IITFLTTDD37 pKa = 3.14 VVKK40 pKa = 10.74 RR41 pKa = 11.84 LRR43 pKa = 11.84 QFQNGDD49 pKa = 3.51 RR50 pKa = 11.84 EE51 pKa = 4.54 CYY53 pKa = 9.83 AVQLLFPLTGWCPSVDD69 pKa = 3.77 VVDD72 pKa = 4.07 GTSYY76 pKa = 9.57 NTLGKK81 pKa = 10.24 LINLIQTSCGLLARR95 pKa = 11.84 QLNVRR100 pKa = 11.84 YY101 pKa = 8.73 PLVGAANAIVNSLVITQLVDD121 pKa = 3.06 CAIRR125 pKa = 11.84 HH126 pKa = 5.89 EE127 pKa = 4.51 STAALIEE134 pKa = 4.4 HH135 pKa = 7.1 LFDD138 pKa = 6.11 DD139 pKa = 4.42 NGQISSLTVHH149 pKa = 6.33 ATTWDD154 pKa = 3.57 EE155 pKa = 4.33 VKK157 pKa = 10.45 LSKK160 pKa = 11.17 NMTVRR165 pKa = 11.84 RR166 pKa = 11.84 RR167 pKa = 11.84 VVEE170 pKa = 4.09 CVAGLKK176 pKa = 9.99 YY177 pKa = 10.14 WLFRR181 pKa = 11.84 NVKK184 pKa = 8.54 GAKK187 pKa = 9.97 SFEE190 pKa = 4.36 TWGKK194 pKa = 10.53 DD195 pKa = 3.36 YY196 pKa = 10.95 PGYY199 pKa = 11.13 ANVHH203 pKa = 6.04 FFDD206 pKa = 5.98 DD207 pKa = 4.1 SAGKK211 pKa = 9.14 QAAIRR216 pKa = 11.84 HH217 pKa = 6.0 IGNDD221 pKa = 3.02 VHH223 pKa = 8.4 IFQHH227 pKa = 6.37 FDD229 pKa = 3.0 NPTYY233 pKa = 10.7 APHH236 pKa = 7.12 LYY238 pKa = 10.49 VPLEE242 pKa = 4.23 GNYY245 pKa = 10.09 SRR247 pKa = 11.84 DD248 pKa = 3.27 MYY250 pKa = 10.65 TDD252 pKa = 3.26 SFSTLVQMEE261 pKa = 4.53 CVVDD265 pKa = 3.61 QARR268 pKa = 11.84 ANSNSGLKK276 pKa = 9.01 MVSRR280 pKa = 11.84 RR281 pKa = 11.84 FIEE284 pKa = 4.05 VMKK287 pKa = 10.31 CIQRR291 pKa = 11.84 PMGEE295 pKa = 4.13 TGVSILSKK303 pKa = 10.29 LDD305 pKa = 3.88 EE306 pKa = 5.17 IGTVLANGGQFEE318 pKa = 5.07 LATLDD323 pKa = 3.42 LSRR326 pKa = 11.84 RR327 pKa = 11.84 EE328 pKa = 4.56 VIHH331 pKa = 7.45 SMIDD335 pKa = 3.71 TISDD339 pKa = 3.69 TPNSSRR345 pKa = 11.84 AIPFDD350 pKa = 3.15 ATRR353 pKa = 11.84 LVIFLDD359 pKa = 3.73 TAYY362 pKa = 9.08 TGPMPSTDD370 pKa = 4.09 FNVSTYY376 pKa = 10.82 EE377 pKa = 4.04 FGFSLIGSVSGKK389 pKa = 10.33 AFSRR393 pKa = 11.84 PIRR396 pKa = 11.84 YY397 pKa = 9.27 SPNYY401 pKa = 9.91 KK402 pKa = 10.22 DD403 pKa = 5.53 DD404 pKa = 5.27 LGDD407 pKa = 3.46 LHH409 pKa = 7.23 DD410 pKa = 3.93 VRR412 pKa = 11.84 EE413 pKa = 4.12 LLRR416 pKa = 11.84 TFVKK420 pKa = 10.56 RR421 pKa = 11.84 KK422 pKa = 10.23 DD423 pKa = 3.53 DD424 pKa = 3.68 VTISNIWDD432 pKa = 3.68 GLPLVDD438 pKa = 5.12 FAKK441 pKa = 10.61 FGNAAATPVDD451 pKa = 3.22 PRR453 pKa = 11.84 LRR455 pKa = 11.84 KK456 pKa = 9.19 EE457 pKa = 3.98 FPNDD461 pKa = 3.07 YY462 pKa = 10.32 FDD464 pKa = 5.34 RR465 pKa = 11.84 EE466 pKa = 3.9 QSINRR471 pKa = 11.84 MLFRR475 pKa = 11.84 GYY477 pKa = 10.46 RR478 pKa = 11.84 KK479 pKa = 8.68 TIDD482 pKa = 3.39 RR483 pKa = 11.84 SWAKK487 pKa = 10.45 DD488 pKa = 3.31 QAVLEE493 pKa = 4.81 TIFSIAGNWLTANKK507 pKa = 9.78 SYY509 pKa = 9.4 TAAYY513 pKa = 9.14 FGASGIHH520 pKa = 6.61 PNDD523 pKa = 4.07 DD524 pKa = 3.37 QPLVIDD530 pKa = 4.32 PWSKK534 pKa = 9.46 GTIFGVPAPSSKK546 pKa = 10.0 VSQYY550 pKa = 11.41 GYY552 pKa = 10.57 DD553 pKa = 3.32 VSNGVITDD561 pKa = 3.78 LTRR564 pKa = 11.84 PSPSGTFSFIYY575 pKa = 10.33 CDD577 pKa = 3.17 VDD579 pKa = 3.64 QVQDD583 pKa = 3.49 AGDD586 pKa = 4.08 DD587 pKa = 3.82 LGVCYY592 pKa = 10.32 QIVRR596 pKa = 11.84 SLFDD600 pKa = 4.01 TINDD604 pKa = 3.71 ALTTGGSFVMKK615 pKa = 10.17 INFPTRR621 pKa = 11.84 QIMDD625 pKa = 3.69 YY626 pKa = 9.7 LVEE629 pKa = 4.23 VVAPKK634 pKa = 9.45 FTDD637 pKa = 3.84 GVLIKK642 pKa = 10.42 PVVSNNLEE650 pKa = 3.97 LFVGFFCKK658 pKa = 10.13 VDD660 pKa = 3.24 NRR662 pKa = 11.84 GCHH665 pKa = 5.41 WNSDD669 pKa = 3.46 CSRR672 pKa = 11.84 FMFRR676 pKa = 11.84 LHH678 pKa = 6.14 NRR680 pKa = 11.84 YY681 pKa = 8.6 NHH683 pKa = 7.09 LDD685 pKa = 3.61 HH686 pKa = 7.11 ACDD689 pKa = 3.99 YY690 pKa = 11.16 IPIIGNARR698 pKa = 11.84 EE699 pKa = 3.99 HH700 pKa = 6.52 PRR702 pKa = 11.84 AISRR706 pKa = 11.84 QEE708 pKa = 3.5 FAIRR712 pKa = 11.84 NPTSSSDD719 pKa = 3.45 TLSQEE724 pKa = 3.92 IEE726 pKa = 3.95 LSLGLFSQQCAANTITISRR745 pKa = 11.84 NLLHH749 pKa = 7.01 GMTEE753 pKa = 3.95 ILVSGVVTASSLNRR767 pKa = 11.84 CEE769 pKa = 4.26 RR770 pKa = 11.84 LDD772 pKa = 3.75 YY773 pKa = 11.28 SPTIDD778 pKa = 3.32 STTILHH784 pKa = 4.99 QHH786 pKa = 6.17 RR787 pKa = 11.84 EE788 pKa = 3.83 IATASPQLFQFEE800 pKa = 4.16 ASEE803 pKa = 4.12 WTLLAMGYY811 pKa = 10.06 NEE813 pKa = 3.75 LAARR817 pKa = 11.84 FVNGSAKK824 pKa = 10.56 SLVDD828 pKa = 3.42 VGSGPEE834 pKa = 3.9 GRR836 pKa = 11.84 SINYY840 pKa = 8.71 VDD842 pKa = 4.51 SDD844 pKa = 3.6 IKK846 pKa = 10.7 VTLFDD851 pKa = 4.13 QRR853 pKa = 11.84 TPHH856 pKa = 7.15 INVDD860 pKa = 2.81 WFANVEE866 pKa = 4.28 YY867 pKa = 10.53 IQGDD871 pKa = 3.71 YY872 pKa = 10.51 LQRR875 pKa = 11.84 RR876 pKa = 11.84 DD877 pKa = 3.14 WRR879 pKa = 11.84 GCTFDD884 pKa = 3.26 TAICIFSFGAATAGSPTGMIEE905 pKa = 4.11 YY906 pKa = 8.8 LTEE909 pKa = 4.0 LLEE912 pKa = 4.05 ILKK915 pKa = 10.54 DD916 pKa = 3.65 AGCTRR921 pKa = 11.84 IIIQLNCPLMTKK933 pKa = 8.69 PTGVVSKK940 pKa = 11.13 LEE942 pKa = 3.71 IDD944 pKa = 4.55 VINDD948 pKa = 3.06 DD949 pKa = 4.48 YY950 pKa = 11.99 YY951 pKa = 10.98 FIKK954 pKa = 10.14 QGRR957 pKa = 11.84 VEE959 pKa = 4.92 PYY961 pKa = 10.65 ASPQDD966 pKa = 3.27 ILGAITQALPQSTVQIKK983 pKa = 8.34 TLDD986 pKa = 4.33 DD987 pKa = 3.76 EE988 pKa = 5.76 LSWFPRR994 pKa = 11.84 IISEE998 pKa = 4.27 GFRR1001 pKa = 11.84 VTTEE1005 pKa = 3.53 AMRR1008 pKa = 11.84 DD1009 pKa = 3.6 AITLSKK1015 pKa = 10.64 LLPLFLIEE1023 pKa = 4.04 TSKK1026 pKa = 10.0 TLFRR1030 pKa = 11.84 PAKK1033 pKa = 10.1 YY1034 pKa = 9.43 IGLVDD1039 pKa = 3.93 EE1040 pKa = 5.59 VITATWTVTDD1050 pKa = 3.94 PFVDD1054 pKa = 3.5 VSVYY1058 pKa = 11.08 LEE1060 pKa = 4.1 DD1061 pKa = 3.54 TSVGFFNTIDD1071 pKa = 3.6 NEE1073 pKa = 4.4 IVGVEE1078 pKa = 4.1 VKK1080 pKa = 10.66 AVFDD1084 pKa = 3.5 GRR1086 pKa = 11.84 GTYY1089 pKa = 10.22 RR1090 pKa = 11.84 GTFSTDD1096 pKa = 2.5 KK1097 pKa = 10.9 AGVVTFEE1104 pKa = 4.07 QTEE1107 pKa = 4.07 KK1108 pKa = 11.1 DD1109 pKa = 3.5 GTSTILGSFLCVTGPNAVAITWPANEE1135 pKa = 4.18 VVGDD1139 pKa = 3.95 NPNVASLTNNTGYY1152 pKa = 10.68 EE1153 pKa = 4.14 LIVAYY1158 pKa = 9.58 EE1159 pKa = 4.03 YY1160 pKa = 10.93 DD1161 pKa = 3.89 GTWIGVNAYY1170 pKa = 8.68 KK1171 pKa = 11.0 ANVYY1175 pKa = 10.22 EE1176 pKa = 5.14 DD1177 pKa = 3.57 AAGDD1181 pKa = 3.78 DD1182 pKa = 3.36 KK1183 pKa = 11.27 MEE1185 pKa = 4.37 YY1186 pKa = 8.02 YY1187 pKa = 10.55 HH1188 pKa = 7.2 VVGEE1192 pKa = 4.67 EE1193 pKa = 3.73 KK1194 pKa = 10.71 LAWALVDD1201 pKa = 3.14 HH1202 pKa = 7.2 HH1203 pKa = 7.21 YY1204 pKa = 10.75 GSPGARR1210 pKa = 11.84 VVIPFVWPDD1219 pKa = 3.13 VTALPGDD1226 pKa = 4.06 VLVAPPYY1233 pKa = 10.66 AGDD1236 pKa = 3.23 WLVNVDD1242 pKa = 3.9 GNLTAEE1248 pKa = 4.24 LHH1250 pKa = 5.72 VDD1252 pKa = 3.71 EE1253 pKa = 5.7 PDD1255 pKa = 4.1 EE1256 pKa = 5.53 IPALWTLMTRR1266 pKa = 11.84 SVANNGSSLSYY1277 pKa = 10.08 IGQAGIYY1284 pKa = 9.02 TFLKK1288 pKa = 10.76 LPP1290 pKa = 3.92
Molecular weight: 143.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.863
IPC2_protein 4.914
IPC_protein 4.902
Toseland 4.8
ProMoST 5.054
Dawson 4.902
Bjellqvist 5.029
Wikipedia 4.8
Rodwell 4.787
Grimsley 4.724
Solomon 4.902
Lehninger 4.863
Nozaki 5.016
DTASelect 5.219
Thurlkill 4.813
EMBOSS 4.825
Sillero 5.08
Patrickios 3.77
IPC_peptide 4.902
IPC2_peptide 5.067
IPC2.peptide.svr19 5.018
Protein with the highest isoelectric point:
>tr|S5LJC9|S5LJC9_9REOV Outer fiber OS=Piscine orthoreovirus OX=1157337 PE=2 SV=1
MM1 pKa = 7.38 ARR3 pKa = 11.84 AIFSGISAFFANAPYY18 pKa = 10.53 VQDD21 pKa = 3.43 GDD23 pKa = 4.41 TIKK26 pKa = 10.73 HH27 pKa = 6.0 AFLSGDD33 pKa = 3.5 SLFFQGTNTLYY44 pKa = 7.9 PTLSTSYY51 pKa = 10.6 QGDD54 pKa = 3.57 TDD56 pKa = 4.49 LPTPFTVMYY65 pKa = 7.21 QTAMVRR71 pKa = 11.84 SALFQVPLFGGLWNARR87 pKa = 11.84 SYY89 pKa = 11.42 RR90 pKa = 11.84 DD91 pKa = 4.18 LVFTSQAMLNVKK103 pKa = 9.12 TNTSVTCPPPVIPRR117 pKa = 11.84 PAYY120 pKa = 10.13 VYY122 pKa = 11.08 NVMNNQRR129 pKa = 11.84 FAQSATARR137 pKa = 11.84 NKK139 pKa = 9.89 VYY141 pKa = 11.0 VDD143 pKa = 4.49 FSITTLFQMDD153 pKa = 3.5 INGFALPLLFNPDD166 pKa = 3.59 DD167 pKa = 4.04 NGIDD171 pKa = 3.37 VTLALTSLVGQSWSTIVGARR191 pKa = 11.84 YY192 pKa = 9.15 EE193 pKa = 4.45 SAGNAAMDD201 pKa = 3.86 IDD203 pKa = 4.26 NPIHH207 pKa = 5.53 RR208 pKa = 11.84 TNRR211 pKa = 11.84 ALMLLYY217 pKa = 10.51 LGSACGYY224 pKa = 8.69 FNPTMTWNGFYY235 pKa = 10.25 FRR237 pKa = 11.84 QAGKK241 pKa = 9.19 PGSWGADD248 pKa = 3.16 LDD250 pKa = 4.93 PILVRR255 pKa = 11.84 GDD257 pKa = 3.22 SALINRR263 pKa = 11.84 ATFVRR268 pKa = 11.84 LNRR271 pKa = 11.84 WVVFKK276 pKa = 11.23 DD277 pKa = 3.86 FLWQMSRR284 pKa = 11.84 GTLHH288 pKa = 6.95 ALVLGGMICAVEE300 pKa = 4.01 QPLRR304 pKa = 11.84 GLSVISVLANTVCAPWTGVNGRR326 pKa = 11.84 AGDD329 pKa = 3.79 EE330 pKa = 4.12 VTTIGLKK337 pKa = 10.18 YY338 pKa = 10.27 VAIEE342 pKa = 3.66 NLIRR346 pKa = 11.84 SGSYY350 pKa = 8.44 TVAEE354 pKa = 4.44 GVVADD359 pKa = 4.36 AQIAAWGVRR368 pKa = 11.84 NTDD371 pKa = 2.34 HH372 pKa = 7.15 MDD374 pKa = 3.3 RR375 pKa = 11.84 VRR377 pKa = 11.84 AADD380 pKa = 3.94 DD381 pKa = 4.1 ANVLAGVNIRR391 pKa = 11.84 RR392 pKa = 11.84 AKK394 pKa = 10.05 PWDD397 pKa = 3.39 NGGGFQRR404 pKa = 11.84 LAAVRR409 pKa = 11.84 ALVNLMAANTRR420 pKa = 3.62
Molecular weight: 45.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.337
IPC2_protein 8.317
IPC_protein 8.404
Toseland 8.375
ProMoST 8.829
Dawson 8.887
Bjellqvist 9.019
Wikipedia 9.004
Rodwell 8.902
Grimsley 8.478
Solomon 9.048
Lehninger 9.004
Nozaki 8.99
DTASelect 8.843
Thurlkill 8.843
EMBOSS 9.019
Sillero 9.092
Patrickios 4.317
IPC_peptide 9.033
IPC2_peptide 8.097
IPC2.peptide.svr19 8.025
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11
0
11
7599
124
1290
690.8
76.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.317 ± 0.48
1.342 ± 0.142
6.238 ± 0.339
4.356 ± 0.264
3.961 ± 0.329
6.159 ± 0.392
1.856 ± 0.213
5.948 ± 0.211
4.237 ± 0.404
9.396 ± 0.315
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.921 ± 0.3
4.448 ± 0.293
5.316 ± 0.341
3.5 ± 0.209
5.303 ± 0.315
8.093 ± 0.375
7.369 ± 0.374
7.593 ± 0.352
1.5 ± 0.131
3.145 ± 0.238
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here