Piscine orthoreovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Spinareovirinae; Orthoreovirus

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0S2EGR3|A0A0S2EGR3_9REOV RNA-directed RNA polymerase (Fragment) OS=Piscine orthoreovirus OX=1157337 PE=2 SV=1
MM1 pKa = 7.02ATLYY5 pKa = 10.73GLRR8 pKa = 11.84ITRR11 pKa = 11.84LKK13 pKa = 10.65NLPLLEE19 pKa = 5.31RR20 pKa = 11.84DD21 pKa = 3.68TEE23 pKa = 4.25EE24 pKa = 3.86YY25 pKa = 9.35TYY27 pKa = 11.39KK28 pKa = 10.78EE29 pKa = 4.23IITFLTTDD37 pKa = 3.14VVKK40 pKa = 10.74RR41 pKa = 11.84LRR43 pKa = 11.84QFQNGDD49 pKa = 3.51RR50 pKa = 11.84EE51 pKa = 4.54CYY53 pKa = 9.83AVQLLFPLTGWCPSVDD69 pKa = 3.77VVDD72 pKa = 4.07GTSYY76 pKa = 9.57NTLGKK81 pKa = 10.24LINLIQTSCGLLARR95 pKa = 11.84QLNVRR100 pKa = 11.84YY101 pKa = 8.73PLVGAANAIVNSLVITQLVDD121 pKa = 3.06CAIRR125 pKa = 11.84HH126 pKa = 5.89EE127 pKa = 4.51STAALIEE134 pKa = 4.4HH135 pKa = 7.1LFDD138 pKa = 6.11DD139 pKa = 4.42NGQISSLTVHH149 pKa = 6.33ATTWDD154 pKa = 3.57EE155 pKa = 4.33VKK157 pKa = 10.45LSKK160 pKa = 11.17NMTVRR165 pKa = 11.84RR166 pKa = 11.84RR167 pKa = 11.84VVEE170 pKa = 4.09CVAGLKK176 pKa = 9.99YY177 pKa = 10.14WLFRR181 pKa = 11.84NVKK184 pKa = 8.54GAKK187 pKa = 9.97SFEE190 pKa = 4.36TWGKK194 pKa = 10.53DD195 pKa = 3.36YY196 pKa = 10.95PGYY199 pKa = 11.13ANVHH203 pKa = 6.04FFDD206 pKa = 5.98DD207 pKa = 4.1SAGKK211 pKa = 9.14QAAIRR216 pKa = 11.84HH217 pKa = 6.0IGNDD221 pKa = 3.02VHH223 pKa = 8.4IFQHH227 pKa = 6.37FDD229 pKa = 3.0NPTYY233 pKa = 10.7APHH236 pKa = 7.12LYY238 pKa = 10.49VPLEE242 pKa = 4.23GNYY245 pKa = 10.09SRR247 pKa = 11.84DD248 pKa = 3.27MYY250 pKa = 10.65TDD252 pKa = 3.26SFSTLVQMEE261 pKa = 4.53CVVDD265 pKa = 3.61QARR268 pKa = 11.84ANSNSGLKK276 pKa = 9.01MVSRR280 pKa = 11.84RR281 pKa = 11.84FIEE284 pKa = 4.05VMKK287 pKa = 10.31CIQRR291 pKa = 11.84PMGEE295 pKa = 4.13TGVSILSKK303 pKa = 10.29LDD305 pKa = 3.88EE306 pKa = 5.17IGTVLANGGQFEE318 pKa = 5.07LATLDD323 pKa = 3.42LSRR326 pKa = 11.84RR327 pKa = 11.84EE328 pKa = 4.56VIHH331 pKa = 7.45SMIDD335 pKa = 3.71TISDD339 pKa = 3.69TPNSSRR345 pKa = 11.84AIPFDD350 pKa = 3.15ATRR353 pKa = 11.84LVIFLDD359 pKa = 3.73TAYY362 pKa = 9.08TGPMPSTDD370 pKa = 4.09FNVSTYY376 pKa = 10.82EE377 pKa = 4.04FGFSLIGSVSGKK389 pKa = 10.33AFSRR393 pKa = 11.84PIRR396 pKa = 11.84YY397 pKa = 9.27SPNYY401 pKa = 9.91KK402 pKa = 10.22DD403 pKa = 5.53DD404 pKa = 5.27LGDD407 pKa = 3.46LHH409 pKa = 7.23DD410 pKa = 3.93VRR412 pKa = 11.84EE413 pKa = 4.12LLRR416 pKa = 11.84TFVKK420 pKa = 10.56RR421 pKa = 11.84KK422 pKa = 10.23DD423 pKa = 3.53DD424 pKa = 3.68VTISNIWDD432 pKa = 3.68GLPLVDD438 pKa = 5.12FAKK441 pKa = 10.61FGNAAATPVDD451 pKa = 3.22PRR453 pKa = 11.84LRR455 pKa = 11.84KK456 pKa = 9.19EE457 pKa = 3.98FPNDD461 pKa = 3.07YY462 pKa = 10.32FDD464 pKa = 5.34RR465 pKa = 11.84EE466 pKa = 3.9QSINRR471 pKa = 11.84MLFRR475 pKa = 11.84GYY477 pKa = 10.46RR478 pKa = 11.84KK479 pKa = 8.68TIDD482 pKa = 3.39RR483 pKa = 11.84SWAKK487 pKa = 10.45DD488 pKa = 3.31QAVLEE493 pKa = 4.81TIFSIAGNWLTANKK507 pKa = 9.78SYY509 pKa = 9.4TAAYY513 pKa = 9.14FGASGIHH520 pKa = 6.61PNDD523 pKa = 4.07DD524 pKa = 3.37QPLVIDD530 pKa = 4.32PWSKK534 pKa = 9.46GTIFGVPAPSSKK546 pKa = 10.0VSQYY550 pKa = 11.41GYY552 pKa = 10.57DD553 pKa = 3.32VSNGVITDD561 pKa = 3.78LTRR564 pKa = 11.84PSPSGTFSFIYY575 pKa = 10.33CDD577 pKa = 3.17VDD579 pKa = 3.64QVQDD583 pKa = 3.49AGDD586 pKa = 4.08DD587 pKa = 3.82LGVCYY592 pKa = 10.32QIVRR596 pKa = 11.84SLFDD600 pKa = 4.01TINDD604 pKa = 3.71ALTTGGSFVMKK615 pKa = 10.17INFPTRR621 pKa = 11.84QIMDD625 pKa = 3.69YY626 pKa = 9.7LVEE629 pKa = 4.23VVAPKK634 pKa = 9.45FTDD637 pKa = 3.84GVLIKK642 pKa = 10.42PVVSNNLEE650 pKa = 3.97LFVGFFCKK658 pKa = 10.13VDD660 pKa = 3.24NRR662 pKa = 11.84GCHH665 pKa = 5.41WNSDD669 pKa = 3.46CSRR672 pKa = 11.84FMFRR676 pKa = 11.84LHH678 pKa = 6.14NRR680 pKa = 11.84YY681 pKa = 8.6NHH683 pKa = 7.09LDD685 pKa = 3.61HH686 pKa = 7.11ACDD689 pKa = 3.99YY690 pKa = 11.16IPIIGNARR698 pKa = 11.84EE699 pKa = 3.99HH700 pKa = 6.52PRR702 pKa = 11.84AISRR706 pKa = 11.84QEE708 pKa = 3.5FAIRR712 pKa = 11.84NPTSSSDD719 pKa = 3.45TLSQEE724 pKa = 3.92IEE726 pKa = 3.95LSLGLFSQQCAANTITISRR745 pKa = 11.84NLLHH749 pKa = 7.01GMTEE753 pKa = 3.95ILVSGVVTASSLNRR767 pKa = 11.84CEE769 pKa = 4.26RR770 pKa = 11.84LDD772 pKa = 3.75YY773 pKa = 11.28SPTIDD778 pKa = 3.32STTILHH784 pKa = 4.99QHH786 pKa = 6.17RR787 pKa = 11.84EE788 pKa = 3.83IATASPQLFQFEE800 pKa = 4.16ASEE803 pKa = 4.12WTLLAMGYY811 pKa = 10.06NEE813 pKa = 3.75LAARR817 pKa = 11.84FVNGSAKK824 pKa = 10.56SLVDD828 pKa = 3.42VGSGPEE834 pKa = 3.9GRR836 pKa = 11.84SINYY840 pKa = 8.71VDD842 pKa = 4.51SDD844 pKa = 3.6IKK846 pKa = 10.7VTLFDD851 pKa = 4.13QRR853 pKa = 11.84TPHH856 pKa = 7.15INVDD860 pKa = 2.81WFANVEE866 pKa = 4.28YY867 pKa = 10.53IQGDD871 pKa = 3.71YY872 pKa = 10.51LQRR875 pKa = 11.84RR876 pKa = 11.84DD877 pKa = 3.14WRR879 pKa = 11.84GCTFDD884 pKa = 3.26TAICIFSFGAATAGSPTGMIEE905 pKa = 4.11YY906 pKa = 8.8LTEE909 pKa = 4.0LLEE912 pKa = 4.05ILKK915 pKa = 10.54DD916 pKa = 3.65AGCTRR921 pKa = 11.84IIIQLNCPLMTKK933 pKa = 8.69PTGVVSKK940 pKa = 11.13LEE942 pKa = 3.71IDD944 pKa = 4.55VINDD948 pKa = 3.06DD949 pKa = 4.48YY950 pKa = 11.99YY951 pKa = 10.98FIKK954 pKa = 10.14QGRR957 pKa = 11.84VEE959 pKa = 4.92PYY961 pKa = 10.65ASPQDD966 pKa = 3.27ILGAITQALPQSTVQIKK983 pKa = 8.34TLDD986 pKa = 4.33DD987 pKa = 3.76EE988 pKa = 5.76LSWFPRR994 pKa = 11.84IISEE998 pKa = 4.27GFRR1001 pKa = 11.84VTTEE1005 pKa = 3.53AMRR1008 pKa = 11.84DD1009 pKa = 3.6AITLSKK1015 pKa = 10.64LLPLFLIEE1023 pKa = 4.04TSKK1026 pKa = 10.0TLFRR1030 pKa = 11.84PAKK1033 pKa = 10.1YY1034 pKa = 9.43IGLVDD1039 pKa = 3.93EE1040 pKa = 5.59VITATWTVTDD1050 pKa = 3.94PFVDD1054 pKa = 3.5VSVYY1058 pKa = 11.08LEE1060 pKa = 4.1DD1061 pKa = 3.54TSVGFFNTIDD1071 pKa = 3.6NEE1073 pKa = 4.4IVGVEE1078 pKa = 4.1VKK1080 pKa = 10.66AVFDD1084 pKa = 3.5GRR1086 pKa = 11.84GTYY1089 pKa = 10.22RR1090 pKa = 11.84GTFSTDD1096 pKa = 2.5KK1097 pKa = 10.9AGVVTFEE1104 pKa = 4.07QTEE1107 pKa = 4.07KK1108 pKa = 11.1DD1109 pKa = 3.5GTSTILGSFLCVTGPNAVAITWPANEE1135 pKa = 4.18VVGDD1139 pKa = 3.95NPNVASLTNNTGYY1152 pKa = 10.68EE1153 pKa = 4.14LIVAYY1158 pKa = 9.58EE1159 pKa = 4.03YY1160 pKa = 10.93DD1161 pKa = 3.89GTWIGVNAYY1170 pKa = 8.68KK1171 pKa = 11.0ANVYY1175 pKa = 10.22EE1176 pKa = 5.14DD1177 pKa = 3.57AAGDD1181 pKa = 3.78DD1182 pKa = 3.36KK1183 pKa = 11.27MEE1185 pKa = 4.37YY1186 pKa = 8.02YY1187 pKa = 10.55HH1188 pKa = 7.2VVGEE1192 pKa = 4.67EE1193 pKa = 3.73KK1194 pKa = 10.71LAWALVDD1201 pKa = 3.14HH1202 pKa = 7.2HH1203 pKa = 7.21YY1204 pKa = 10.75GSPGARR1210 pKa = 11.84VVIPFVWPDD1219 pKa = 3.13VTALPGDD1226 pKa = 4.06VLVAPPYY1233 pKa = 10.66AGDD1236 pKa = 3.23WLVNVDD1242 pKa = 3.9GNLTAEE1248 pKa = 4.24LHH1250 pKa = 5.72VDD1252 pKa = 3.71EE1253 pKa = 5.7PDD1255 pKa = 4.1EE1256 pKa = 5.53IPALWTLMTRR1266 pKa = 11.84SVANNGSSLSYY1277 pKa = 10.08IGQAGIYY1284 pKa = 9.02TFLKK1288 pKa = 10.76LPP1290 pKa = 3.92

Molecular weight:
143.7 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|S5LJC9|S5LJC9_9REOV Outer fiber OS=Piscine orthoreovirus OX=1157337 PE=2 SV=1
MM1 pKa = 7.38ARR3 pKa = 11.84AIFSGISAFFANAPYY18 pKa = 10.53VQDD21 pKa = 3.43GDD23 pKa = 4.41TIKK26 pKa = 10.73HH27 pKa = 6.0AFLSGDD33 pKa = 3.5SLFFQGTNTLYY44 pKa = 7.9PTLSTSYY51 pKa = 10.6QGDD54 pKa = 3.57TDD56 pKa = 4.49LPTPFTVMYY65 pKa = 7.21QTAMVRR71 pKa = 11.84SALFQVPLFGGLWNARR87 pKa = 11.84SYY89 pKa = 11.42RR90 pKa = 11.84DD91 pKa = 4.18LVFTSQAMLNVKK103 pKa = 9.12TNTSVTCPPPVIPRR117 pKa = 11.84PAYY120 pKa = 10.13VYY122 pKa = 11.08NVMNNQRR129 pKa = 11.84FAQSATARR137 pKa = 11.84NKK139 pKa = 9.89VYY141 pKa = 11.0VDD143 pKa = 4.49FSITTLFQMDD153 pKa = 3.5INGFALPLLFNPDD166 pKa = 3.59DD167 pKa = 4.04NGIDD171 pKa = 3.37VTLALTSLVGQSWSTIVGARR191 pKa = 11.84YY192 pKa = 9.15EE193 pKa = 4.45SAGNAAMDD201 pKa = 3.86IDD203 pKa = 4.26NPIHH207 pKa = 5.53RR208 pKa = 11.84TNRR211 pKa = 11.84ALMLLYY217 pKa = 10.51LGSACGYY224 pKa = 8.69FNPTMTWNGFYY235 pKa = 10.25FRR237 pKa = 11.84QAGKK241 pKa = 9.19PGSWGADD248 pKa = 3.16LDD250 pKa = 4.93PILVRR255 pKa = 11.84GDD257 pKa = 3.22SALINRR263 pKa = 11.84ATFVRR268 pKa = 11.84LNRR271 pKa = 11.84WVVFKK276 pKa = 11.23DD277 pKa = 3.86FLWQMSRR284 pKa = 11.84GTLHH288 pKa = 6.95ALVLGGMICAVEE300 pKa = 4.01QPLRR304 pKa = 11.84GLSVISVLANTVCAPWTGVNGRR326 pKa = 11.84AGDD329 pKa = 3.79EE330 pKa = 4.12VTTIGLKK337 pKa = 10.18YY338 pKa = 10.27VAIEE342 pKa = 3.66NLIRR346 pKa = 11.84SGSYY350 pKa = 8.44TVAEE354 pKa = 4.44GVVADD359 pKa = 4.36AQIAAWGVRR368 pKa = 11.84NTDD371 pKa = 2.34HH372 pKa = 7.15MDD374 pKa = 3.3RR375 pKa = 11.84VRR377 pKa = 11.84AADD380 pKa = 3.94DD381 pKa = 4.1ANVLAGVNIRR391 pKa = 11.84RR392 pKa = 11.84AKK394 pKa = 10.05PWDD397 pKa = 3.39NGGGFQRR404 pKa = 11.84LAAVRR409 pKa = 11.84ALVNLMAANTRR420 pKa = 3.62

Molecular weight:
45.89 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

7599

124

1290

690.8

76.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.317 ± 0.48

1.342 ± 0.142

6.238 ± 0.339

4.356 ± 0.264

3.961 ± 0.329

6.159 ± 0.392

1.856 ± 0.213

5.948 ± 0.211

4.237 ± 0.404

9.396 ± 0.315

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.921 ± 0.3

4.448 ± 0.293

5.316 ± 0.341

3.5 ± 0.209

5.303 ± 0.315

8.093 ± 0.375

7.369 ± 0.374

7.593 ± 0.352

1.5 ± 0.131

3.145 ± 0.238

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski