Gokushovirinae Fen672_31

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; Gokushovirinae; unclassified Gokushovirinae

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G2UMF8|A0A0G2UMF8_9VIRU Internal scaffolding protein VP3 OS=Gokushovirinae Fen672_31 OX=1655656 PE=4 SV=1
MM1 pKa = 7.58ASDD4 pKa = 3.06KK5 pKa = 11.04RR6 pKa = 11.84GVHH9 pKa = 6.38RR10 pKa = 11.84IEE12 pKa = 4.62SEE14 pKa = 4.02VFHH17 pKa = 6.01YY18 pKa = 7.94EE19 pKa = 3.86KK20 pKa = 10.51PKK22 pKa = 11.07EE23 pKa = 4.05NGLSDD28 pKa = 4.07FYY30 pKa = 11.09KK31 pKa = 9.88PHH33 pKa = 6.72IAVGSPAGGPTLTRR47 pKa = 11.84QEE49 pKa = 4.18FAEE52 pKa = 4.41EE53 pKa = 4.14CDD55 pKa = 3.43VNAIMEE61 pKa = 4.46RR62 pKa = 11.84YY63 pKa = 6.85EE64 pKa = 4.25TTGVLPNVVGQPFYY78 pKa = 11.59YY79 pKa = 10.64DD80 pKa = 3.72FTTLPTDD87 pKa = 3.22MMSAMDD93 pKa = 3.75VMRR96 pKa = 11.84NATDD100 pKa = 3.12AFYY103 pKa = 10.11TLPAKK108 pKa = 9.83VRR110 pKa = 11.84KK111 pKa = 9.82EE112 pKa = 3.97FDD114 pKa = 2.91NDD116 pKa = 2.78AAAFVDD122 pKa = 5.39FASDD126 pKa = 4.11PDD128 pKa = 4.12NLDD131 pKa = 3.24QMRR134 pKa = 11.84SWGLAPQAKK143 pKa = 9.86PEE145 pKa = 4.12EE146 pKa = 4.43PLVAPQAAPGPALAPAAPGPAAAPGGASKK175 pKa = 10.03PAA177 pKa = 3.6

Molecular weight: 19.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G2UMF8|A0A0G2UMF8_9VIRU Internal scaffolding protein VP3 OS=Gokushovirinae Fen672_31 OX=1655656 PE=4 SV=1
MM1 pKa = 7.37SKK3 pKa = 10.29KK4 pKa = 10.34ASRR7 pKa = 11.84KK8 pKa = 9.53SFTKK12 pKa = 10.6GAVKK16 pKa = 9.71VHH18 pKa = 6.29KK19 pKa = 10.26KK20 pKa = 9.86NVPTRR25 pKa = 11.84VPMRR29 pKa = 11.84GGIRR33 pKa = 11.84LL34 pKa = 3.45

Molecular weight: 3.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1404

34

526

200.6

22.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.256 ± 1.31

1.211 ± 0.373

5.342 ± 0.603

3.775 ± 0.531

4.843 ± 0.87

7.692 ± 0.522

1.852 ± 0.338

3.704 ± 0.647

4.202 ± 1.257

6.909 ± 0.614

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.632 ± 0.868

3.989 ± 0.766

6.054 ± 0.986

4.63 ± 1.374

6.339 ± 1.328

7.55 ± 0.923

6.766 ± 0.897

6.838 ± 0.889

0.997 ± 0.324

3.419 ± 0.572
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski