Talaromyces atroroseus
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9520 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A225ACX0|A0A225ACX0_9EURO Protein kinase gsk3 OS=Talaromyces atroroseus OX=1441469 GN=UA08_07899 PE=3 SV=1
MM1 pKa = 7.57 PSKK4 pKa = 9.88 TGLALFGLAASALAEE19 pKa = 4.08 PQVLHH24 pKa = 6.33 MPLTRR29 pKa = 11.84 NPNANRR35 pKa = 11.84 LAKK38 pKa = 10.47 RR39 pKa = 11.84 NGDD42 pKa = 3.67 ALVDD46 pKa = 3.52 ITNDD50 pKa = 3.25 LSEE53 pKa = 3.98 GLYY56 pKa = 10.08 YY57 pKa = 11.2 VNASVGTPGQEE68 pKa = 3.56 IQLTLDD74 pKa = 3.71 TGSSDD79 pKa = 2.37 IWFFGANSCDD89 pKa = 4.22 EE90 pKa = 4.14 NTTDD94 pKa = 5.52 CFGGTYY100 pKa = 10.22 NPSKK104 pKa = 10.43 SSSVTVLNPKK114 pKa = 9.92 GAFSIQYY121 pKa = 7.84 GTAGSNVTGNYY132 pKa = 7.7 ITDD135 pKa = 4.01 DD136 pKa = 4.04 FSVGAASVKK145 pKa = 10.15 NLTMAYY151 pKa = 7.93 ATYY154 pKa = 10.47 AAYY157 pKa = 10.62 VPTGVMGIGFDD168 pKa = 3.65 TNEE171 pKa = 5.1 AITDD175 pKa = 4.06 EE176 pKa = 4.52 YY177 pKa = 10.99 GSPYY181 pKa = 10.28 PNFVDD186 pKa = 3.78 VLVSQNVTKK195 pKa = 10.51 TKK197 pKa = 10.37 AYY199 pKa = 10.47 SLWLNDD205 pKa = 4.18 LDD207 pKa = 4.75 SSTGNILFGGYY218 pKa = 7.58 DD219 pKa = 3.27 TKK221 pKa = 11.17 KK222 pKa = 10.77 FSGEE226 pKa = 4.07 LLTVDD231 pKa = 3.97 IQPDD235 pKa = 3.58 EE236 pKa = 4.09 MSGEE240 pKa = 4.17 ITSMTVAWTSLNVTANSKK258 pKa = 5.72 TTQVTKK264 pKa = 10.96 SDD266 pKa = 3.85 FTSPALLDD274 pKa = 3.68 SGTTITIVPDD284 pKa = 4.38 DD285 pKa = 3.33 IYY287 pKa = 11.47 YY288 pKa = 11.23 ALFEE292 pKa = 4.26 YY293 pKa = 9.94 FQAEE297 pKa = 4.1 SDD299 pKa = 4.18 GEE301 pKa = 3.81 GDD303 pKa = 4.94 AIVKK307 pKa = 10.42 CSLLDD312 pKa = 3.79 SSDD315 pKa = 3.18 GTLDD319 pKa = 3.45 FGFGGTGGPVVKK331 pKa = 10.81 VPFSEE336 pKa = 4.4 LALPATDD343 pKa = 4.83 TNGNWLAFEE352 pKa = 5.07 DD353 pKa = 4.84 GEE355 pKa = 4.45 LACVLGLEE363 pKa = 4.43 GTDD366 pKa = 3.55 DD367 pKa = 4.17 GEE369 pKa = 4.37 MPVIFGDD376 pKa = 3.54 TFLRR380 pKa = 11.84 SAYY383 pKa = 9.93 VVYY386 pKa = 10.88 DD387 pKa = 3.61 LTNKK391 pKa = 10.0 QISLAQTVFNATDD404 pKa = 3.53 SNIVEE409 pKa = 4.53 ISSSSPVASVVTGVTVTQTATNNPLGQATATAASSTPTDD448 pKa = 3.34 SSGSSINSIGVLSSSTSTATSAASTSSHH476 pKa = 5.89 GSAASIPAPVPGLMTSLLVAGASMMFGSVFFMLHH510 pKa = 5.43
Molecular weight: 53.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.711
IPC2_protein 3.846
IPC_protein 3.872
Toseland 3.643
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.808
Rodwell 3.694
Grimsley 3.554
Solomon 3.859
Lehninger 3.821
Nozaki 3.973
DTASelect 4.228
Thurlkill 3.694
EMBOSS 3.808
Sillero 3.999
Patrickios 1.036
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.88
Protein with the highest isoelectric point:
>tr|A0A1Q5Q693|A0A1Q5Q693_9EURO Uncharacterized protein OS=Talaromyces atroroseus OX=1441469 GN=UA08_09365 PE=3 SV=1
MM1 pKa = 8.0 AIPRR5 pKa = 11.84 TPRR8 pKa = 11.84 TMAKK12 pKa = 10.16 HH13 pKa = 5.7 PMPPFLRR20 pKa = 11.84 QEE22 pKa = 4.12 EE23 pKa = 4.34 EE24 pKa = 4.09 KK25 pKa = 10.39 MRR27 pKa = 11.84 MEE29 pKa = 4.81 SPRR32 pKa = 11.84 FACPPKK38 pKa = 9.29 NTAIKK43 pKa = 7.75 TTIKK47 pKa = 7.01 TTLMKK52 pKa = 10.24 PSLISVVHH60 pKa = 6.74 GKK62 pKa = 8.98 RR63 pKa = 11.84 QPWRR67 pKa = 11.84 HH68 pKa = 4.06 MWIPRR73 pKa = 11.84 RR74 pKa = 11.84 PRR76 pKa = 11.84 THH78 pKa = 6.5 CWKK81 pKa = 9.96 SACPGPVQRR90 pKa = 11.84 LLTSVLTRR98 pKa = 11.84 PTGQRR103 pKa = 11.84 SPRR106 pKa = 11.84 NSNYY110 pKa = 9.82 LRR112 pKa = 11.84 LILKK116 pKa = 8.93 CQFWANGARR125 pKa = 11.84 KK126 pKa = 9.58 HH127 pKa = 5.79 ILNVRR132 pKa = 11.84 NGKK135 pKa = 10.25 RR136 pKa = 11.84 IDD138 pKa = 3.67 SQEE141 pKa = 3.8 VLGDD145 pKa = 3.62 STRR148 pKa = 11.84 ATAATKK154 pKa = 9.29 RR155 pKa = 11.84 TAPHH159 pKa = 6.48 HH160 pKa = 7.05 DD161 pKa = 3.97 SYY163 pKa = 11.56 NSPVGRR169 pKa = 11.84 LIGEE173 pKa = 4.1 GRR175 pKa = 11.84 VRR177 pKa = 11.84 SPGLFAIRR185 pKa = 11.84 NDD187 pKa = 3.73 HH188 pKa = 7.44 DD189 pKa = 4.1 GGGYY193 pKa = 8.36 GTDD196 pKa = 3.67 DD197 pKa = 4.0 SGSASASRR205 pKa = 11.84 GSIFSRR211 pKa = 11.84 QSEE214 pKa = 4.02 KK215 pKa = 10.46 RR216 pKa = 11.84 YY217 pKa = 10.1 SGSVTSGEE225 pKa = 4.31 GPSPNSLVDD234 pKa = 3.82 PGAEE238 pKa = 3.41 QSGFSRR244 pKa = 11.84 AAGGQSRR251 pKa = 11.84 VPAEE255 pKa = 4.31 SPSVASVSRR264 pKa = 11.84 QLGSEE269 pKa = 4.08 IEE271 pKa = 4.32 DD272 pKa = 3.73 SEE274 pKa = 4.76 SASDD278 pKa = 3.53 TDD280 pKa = 5.2 DD281 pKa = 4.74 ADD283 pKa = 3.49 HH284 pKa = 7.16 HH285 pKa = 7.34 SGDD288 pKa = 3.54 GRR290 pKa = 11.84 WQYY293 pKa = 11.54 RR294 pKa = 11.84 CILKK298 pKa = 10.32 RR299 pKa = 11.84 RR300 pKa = 11.84 LDD302 pKa = 3.48 SSGRR306 pKa = 11.84 RR307 pKa = 11.84 MALVRR312 pKa = 11.84 WEE314 pKa = 4.36 DD315 pKa = 3.07 TWEE318 pKa = 4.37 SEE320 pKa = 4.99 DD321 pKa = 3.56 EE322 pKa = 4.17 LGGVKK327 pKa = 10.05 RR328 pKa = 11.84 ALRR331 pKa = 11.84 QYY333 pKa = 11.34 ARR335 pKa = 11.84 ARR337 pKa = 11.84 QAKK340 pKa = 8.37 QVPATEE346 pKa = 4.46 TCSKK350 pKa = 10.3 RR351 pKa = 11.84 RR352 pKa = 11.84 GRR354 pKa = 11.84 KK355 pKa = 8.8 RR356 pKa = 11.84 KK357 pKa = 9.9 CPSS360 pKa = 2.78
Molecular weight: 40.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.575
IPC_protein 10.365
Toseland 10.774
ProMoST 10.482
Dawson 10.833
Bjellqvist 10.555
Wikipedia 11.052
Rodwell 11.008
Grimsley 10.877
Solomon 10.994
Lehninger 10.965
Nozaki 10.76
DTASelect 10.555
Thurlkill 10.76
EMBOSS 11.184
Sillero 10.774
Patrickios 10.716
IPC_peptide 10.994
IPC2_peptide 9.663
IPC2.peptide.svr19 8.693
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9520
0
9520
5014686
49
7850
526.8
58.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.365 ± 0.017
1.16 ± 0.009
5.705 ± 0.016
6.164 ± 0.024
3.797 ± 0.012
6.559 ± 0.021
2.401 ± 0.012
5.277 ± 0.016
4.7 ± 0.023
9.046 ± 0.028
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.094 ± 0.009
3.938 ± 0.012
5.666 ± 0.024
4.081 ± 0.016
5.939 ± 0.021
8.632 ± 0.029
6.03 ± 0.02
6.117 ± 0.017
1.427 ± 0.009
2.904 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here