Hydrogenimonas sp.
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2173 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G9HAA2|A0A3G9HAA2_9PROT Cell division trigger factor OS=Hydrogenimonas sp. OX=2231112 GN=NNO_0091 PE=4 SV=1
MM1 pKa = 7.73 TYY3 pKa = 10.92 FLMALIIIFLAAIILIAKK21 pKa = 9.07 YY22 pKa = 10.52 ADD24 pKa = 3.48 SEE26 pKa = 4.3 DD27 pKa = 3.97 MYY29 pKa = 11.65 GEE31 pKa = 4.61 DD32 pKa = 4.13 SGSDD36 pKa = 3.2 RR37 pKa = 11.84 SDD39 pKa = 3.46 DD40 pKa = 3.49
Molecular weight: 4.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.808
IPC2_protein 3.681
IPC_protein 3.554
Toseland 3.363
ProMoST 3.656
Dawson 3.592
Bjellqvist 3.872
Wikipedia 3.605
Rodwell 3.401
Grimsley 3.287
Solomon 3.528
Lehninger 3.478
Nozaki 3.745
DTASelect 3.961
Thurlkill 3.478
EMBOSS 3.605
Sillero 3.694
Patrickios 1.863
IPC_peptide 3.516
IPC2_peptide 3.643
IPC2.peptide.svr19 3.747
Protein with the highest isoelectric point:
>tr|A0A3G9H8I5|A0A3G9H8I5_9PROT Dipeptide epimerase OS=Hydrogenimonas sp. OX=2231112 GN=NNO_0100 PE=3 SV=1
MM1 pKa = 7.46 YY2 pKa = 10.5 DD3 pKa = 4.67 DD4 pKa = 4.23 DD5 pKa = 5.12 TIMAIYY11 pKa = 9.78 DD12 pKa = 3.25 VHH14 pKa = 8.1 RR15 pKa = 11.84 GGISTTMRR23 pKa = 11.84 VKK25 pKa = 9.38 TVKK28 pKa = 10.53 AGTQHH33 pKa = 6.7 KK34 pKa = 10.07 RR35 pKa = 11.84 GTKK38 pKa = 8.92 EE39 pKa = 3.59 SLKK42 pKa = 10.38 RR43 pKa = 11.84 SVPGEE48 pKa = 3.75 RR49 pKa = 11.84 RR50 pKa = 11.84 VKK52 pKa = 10.19 VCRR55 pKa = 11.84 THH57 pKa = 7.83 IDD59 pKa = 2.51 IRR61 pKa = 11.84 QGMRR65 pKa = 11.84 LRR67 pKa = 11.84 YY68 pKa = 9.22 KK69 pKa = 10.58 LPRR72 pKa = 11.84 VCGRR76 pKa = 11.84 MYY78 pKa = 10.97 KK79 pKa = 10.83 VMGLSVNRR87 pKa = 11.84 ASYY90 pKa = 10.65 SGATQRR96 pKa = 11.84 YY97 pKa = 8.33 RR98 pKa = 11.84 EE99 pKa = 4.14 TT100 pKa = 3.48
Molecular weight: 11.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.331
IPC2_protein 9.94
IPC_protein 10.818
Toseland 10.789
ProMoST 10.555
Dawson 10.906
Bjellqvist 10.643
Wikipedia 11.14
Rodwell 11.096
Grimsley 10.965
Solomon 11.023
Lehninger 10.994
Nozaki 10.774
DTASelect 10.643
Thurlkill 10.804
EMBOSS 11.199
Sillero 10.833
Patrickios 10.818
IPC_peptide 11.038
IPC2_peptide 9.706
IPC2.peptide.svr19 8.297
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2171
2
2173
652339
37
2070
300.2
33.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.888 ± 0.052
0.919 ± 0.022
5.48 ± 0.036
8.089 ± 0.067
4.643 ± 0.038
6.934 ± 0.046
1.915 ± 0.023
7.172 ± 0.052
6.901 ± 0.055
9.875 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.726 ± 0.023
3.479 ± 0.036
3.815 ± 0.033
2.382 ± 0.025
5.738 ± 0.051
6.159 ± 0.043
4.563 ± 0.037
6.695 ± 0.044
0.921 ± 0.02
3.705 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here