Ktedonosporobacter rubrisoli
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9315 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V0Z0F6|A0A4V0Z0F6_9CHLR NUDIX domain-containing protein OS=Ktedonosporobacter rubrisoli OX=2509675 GN=EPA93_46660 PE=4 SV=1
MM1 pKa = 7.31 NLEE4 pKa = 3.94 QIKK7 pKa = 10.23 YY8 pKa = 7.25 QTCPWDD14 pKa = 3.39 QEE16 pKa = 4.32 EE17 pKa = 4.97 GVLLTDD23 pKa = 4.32 INEE26 pKa = 4.72 DD27 pKa = 4.85 LPDD30 pKa = 3.64 TDD32 pKa = 5.86 ADD34 pKa = 4.37 GEE36 pKa = 4.24 LQYY39 pKa = 11.49 YY40 pKa = 8.9 CLSGRR45 pKa = 11.84 HH46 pKa = 4.79 VFSYY50 pKa = 11.29 DD51 pKa = 3.24 EE52 pKa = 4.61 DD53 pKa = 4.25 SEE55 pKa = 4.43 NEE57 pKa = 3.65 RR58 pKa = 11.84 RR59 pKa = 11.84 KK60 pKa = 10.47
Molecular weight: 7.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.789
IPC2_protein 3.999
IPC_protein 3.91
Toseland 3.719
ProMoST 4.062
Dawson 3.884
Bjellqvist 4.05
Wikipedia 3.808
Rodwell 3.745
Grimsley 3.63
Solomon 3.872
Lehninger 3.821
Nozaki 4.012
DTASelect 4.19
Thurlkill 3.77
EMBOSS 3.821
Sillero 4.024
Patrickios 1.901
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.92
Protein with the highest isoelectric point:
>tr|A0A4P6K5J2|A0A4P6K5J2_9CHLR Methyltransferase domain-containing protein OS=Ktedonosporobacter rubrisoli OX=2509675 GN=EPA93_47640 PE=4 SV=1
MM1 pKa = 7.88 PGPTGPNPGMMAAQRR16 pKa = 11.84 AAQQAAQQAAQRR28 pKa = 11.84 ASMNAQRR35 pKa = 11.84 AAQQAAQRR43 pKa = 11.84 ASMNAQQAAQRR54 pKa = 11.84 ASVNAQQAARR64 pKa = 11.84 RR65 pKa = 11.84 ASMNAQASLRR75 pKa = 11.84 SAQQTTFRR83 pKa = 11.84 MPLSTRR89 pKa = 11.84 QATNSRR95 pKa = 11.84 SNQTQHH101 pKa = 6.63 ASSNSGFAFGALIAWIVIIGLIVLILWFFFF131 pKa = 4.14
Molecular weight: 13.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.52
IPC2_protein 11.228
IPC_protein 12.837
Toseland 12.998
ProMoST 13.495
Dawson 12.998
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.501
Grimsley 13.042
Solomon 13.495
Lehninger 13.393
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.495
Sillero 12.998
Patrickios 12.237
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9315
0
9315
3002708
12
3235
322.4
35.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.043 ± 0.029
1.037 ± 0.008
4.4 ± 0.017
6.15 ± 0.03
3.68 ± 0.015
6.992 ± 0.027
2.546 ± 0.012
5.813 ± 0.023
3.339 ± 0.018
11.85 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.232 ± 0.01
2.935 ± 0.016
5.055 ± 0.02
5.156 ± 0.024
6.269 ± 0.023
6.116 ± 0.019
5.421 ± 0.017
6.409 ± 0.023
1.522 ± 0.01
3.034 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here