Rhodoferax sediminis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Rhodoferax

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4235 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A515ET31|A0A515ET31_9BURK Histidine kinase OS=Rhodoferax sediminis OX=2509614 GN=EXZ61_17540 PE=4 SV=1
MM1 pKa = 7.13QANPPPNLDD10 pKa = 5.59DD11 pKa = 4.77EE12 pKa = 5.25DD13 pKa = 6.88ADD15 pKa = 4.96DD16 pKa = 5.66GPSACIMVFNANDD29 pKa = 3.33ASGAGGISADD39 pKa = 3.55LTAIASVGAHH49 pKa = 7.06ALPVITGAYY58 pKa = 10.07ARR60 pKa = 11.84DD61 pKa = 3.29TSEE64 pKa = 5.19ILDD67 pKa = 4.27HH68 pKa = 7.07YY69 pKa = 10.46PFDD72 pKa = 5.11DD73 pKa = 4.11EE74 pKa = 5.92AVTEE78 pKa = 4.02QARR81 pKa = 11.84AILEE85 pKa = 4.75DD86 pKa = 3.43IPVDD90 pKa = 3.59VFKK93 pKa = 11.33VGFVGSPEE101 pKa = 4.0NLSAIAEE108 pKa = 4.32LASDD112 pKa = 4.19YY113 pKa = 11.8ADD115 pKa = 4.33LPLVAYY121 pKa = 9.51MPDD124 pKa = 3.73LSWWQEE130 pKa = 3.84DD131 pKa = 4.12LTDD134 pKa = 3.99QYY136 pKa = 11.85HH137 pKa = 6.65DD138 pKa = 3.66ACTEE142 pKa = 3.91LLLPQTTVLVGNYY155 pKa = 7.45STLTRR160 pKa = 11.84WLLPDD165 pKa = 3.87WSTPLPPSALDD176 pKa = 3.46LARR179 pKa = 11.84AANEE183 pKa = 3.76YY184 pKa = 9.5GVPYY188 pKa = 9.96TLVTGMPLPDD198 pKa = 3.24QHH200 pKa = 7.88IDD202 pKa = 3.49NVLCTPEE209 pKa = 5.22AVLCSHH215 pKa = 6.99KK216 pKa = 10.56YY217 pKa = 9.7EE218 pKa = 4.08RR219 pKa = 11.84FEE221 pKa = 4.67AVFSGAGDD229 pKa = 3.75TLSAALAALIASDD242 pKa = 4.09TEE244 pKa = 4.03LVEE247 pKa = 4.47AVGEE251 pKa = 4.0ALSYY255 pKa = 11.16LDD257 pKa = 3.7RR258 pKa = 11.84CLEE261 pKa = 3.95NGFRR265 pKa = 11.84PGMGNVLPDD274 pKa = 3.6RR275 pKa = 11.84LFWAHH280 pKa = 6.8PEE282 pKa = 3.86EE283 pKa = 6.34DD284 pKa = 3.87EE285 pKa = 4.79TDD287 pKa = 3.58DD288 pKa = 5.32ATDD291 pKa = 3.81LDD293 pKa = 4.89SNFEE297 pKa = 3.89ISPNATRR304 pKa = 11.84HH305 pKa = 4.44

Molecular weight: 32.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A515EVA7|A0A515EVA7_9BURK Uncharacterized protein OS=Rhodoferax sediminis OX=2509614 GN=EXZ61_01280 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.07QPSKK9 pKa = 9.07IRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.91GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4235

0

4235

1418997

32

6154

335.1

36.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.323 ± 0.049

0.967 ± 0.011

5.075 ± 0.03

4.996 ± 0.035

3.561 ± 0.026

7.803 ± 0.033

2.372 ± 0.023

4.401 ± 0.025

3.942 ± 0.04

10.695 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.633 ± 0.022

2.974 ± 0.025

4.836 ± 0.033

4.526 ± 0.035

5.786 ± 0.034

5.957 ± 0.029

5.563 ± 0.048

7.778 ± 0.029

1.491 ± 0.018

2.321 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski