Codonopsis vein clearing virus
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A411AUG2|A0A411AUG2_9VIRU ORF2 OS=Codonopsis vein clearing virus OX=2510980 PE=4 SV=1
MM1 pKa = 7.66 RR2 pKa = 11.84 AALEE6 pKa = 4.09 CKK8 pKa = 9.57 EE9 pKa = 4.18 FEE11 pKa = 4.37 EE12 pKa = 5.23 EE13 pKa = 4.14 VEE15 pKa = 3.84 KK16 pKa = 11.02 WEE18 pKa = 4.14 IPEE21 pKa = 4.21 SSTLAGFKK29 pKa = 10.32 EE30 pKa = 4.2 LEE32 pKa = 4.02 QYY34 pKa = 10.67 PNLRR38 pKa = 11.84 KK39 pKa = 9.82 NVVYY43 pKa = 7.33 PTCKK47 pKa = 10.09 LPCFHH52 pKa = 7.25 FSALADD58 pKa = 4.06 PDD60 pKa = 3.38 AHH62 pKa = 6.06 ATFTKK67 pKa = 10.59 RR68 pKa = 11.84 EE69 pKa = 3.97 DD70 pKa = 3.27 RR71 pKa = 11.84 VPFVVNTLFDD81 pKa = 3.88 LAIIHH86 pKa = 5.69 QVNLSYY92 pKa = 10.87 IHH94 pKa = 6.7 TRR96 pKa = 11.84 LQLLSRR102 pKa = 11.84 YY103 pKa = 7.23 QARR106 pKa = 11.84 KK107 pKa = 5.8 TTGAPPLATIPEE119 pKa = 4.36 DD120 pKa = 3.82 RR121 pKa = 11.84 PLPDD125 pKa = 4.19 LSPVVEE131 pKa = 4.38 AVTDD135 pKa = 3.82 SVTGLKK141 pKa = 10.02 SQIVSLKK148 pKa = 10.45 ADD150 pKa = 3.39 LRR152 pKa = 11.84 EE153 pKa = 4.06 IKK155 pKa = 10.48 AGQTSIKK162 pKa = 10.2 LALSDD167 pKa = 3.54 LRR169 pKa = 11.84 EE170 pKa = 4.18 VVTALTEE177 pKa = 4.09 RR178 pKa = 11.84 EE179 pKa = 4.43 LDD181 pKa = 3.78 PKK183 pKa = 10.57 PIEE186 pKa = 4.51 ADD188 pKa = 3.34 TAKK191 pKa = 10.54 VAEE194 pKa = 4.02 QLKK197 pKa = 10.33 VSVKK201 pKa = 10.2 EE202 pKa = 3.99 IQASLKK208 pKa = 10.3 DD209 pKa = 3.77 LKK211 pKa = 11.13 DD212 pKa = 3.55 FAKK215 pKa = 10.77 SLVPEE220 pKa = 4.34 AA221 pKa = 4.62
Molecular weight: 24.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.404
IPC2_protein 5.448
IPC_protein 5.372
Toseland 5.448
ProMoST 5.563
Dawson 5.423
Bjellqvist 5.525
Wikipedia 5.308
Rodwell 5.372
Grimsley 5.41
Solomon 5.423
Lehninger 5.385
Nozaki 5.575
DTASelect 5.715
Thurlkill 5.499
EMBOSS 5.436
Sillero 5.664
Patrickios 4.482
IPC_peptide 5.436
IPC2_peptide 5.664
IPC2.peptide.svr19 5.734
Protein with the highest isoelectric point:
>tr|A0A411AUG2|A0A411AUG2_9VIRU ORF2 OS=Codonopsis vein clearing virus OX=2510980 PE=4 SV=1
MM1 pKa = 7.69 SSWQLAASTDD11 pKa = 3.81 EE12 pKa = 3.84 YY13 pKa = 11.28 RR14 pKa = 11.84 KK15 pKa = 10.31 ALNATGSLTKK25 pKa = 10.46 DD26 pKa = 3.15 NRR28 pKa = 11.84 PVGFVEE34 pKa = 3.84 PHH36 pKa = 6.49 LFQPNLGDD44 pKa = 4.12 TNIQRR49 pKa = 11.84 QNNTLIHH56 pKa = 6.6 LLIQTLEE63 pKa = 4.07 EE64 pKa = 4.2 VKK66 pKa = 10.68 ALRR69 pKa = 11.84 DD70 pKa = 3.3 QVQILRR76 pKa = 11.84 DD77 pKa = 3.74 QVDD80 pKa = 3.69 SLSKK84 pKa = 10.73 GKK86 pKa = 10.44 APEE89 pKa = 3.91 VQLPKK94 pKa = 10.93 DD95 pKa = 3.95 VVDD98 pKa = 4.92 KK99 pKa = 11.16 LAAQLTTTHH108 pKa = 5.54 VTPKK112 pKa = 10.63 GKK114 pKa = 9.94 FRR116 pKa = 11.84 GKK118 pKa = 10.33 GKK120 pKa = 10.19 EE121 pKa = 3.79 GQFRR125 pKa = 11.84 VWSS128 pKa = 3.83
Molecular weight: 14.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.75
IPC2_protein 8.77
IPC_protein 8.756
Toseland 9.882
ProMoST 9.326
Dawson 9.97
Bjellqvist 9.516
Wikipedia 10.058
Rodwell 10.643
Grimsley 9.999
Solomon 10.028
Lehninger 10.028
Nozaki 9.823
DTASelect 9.531
Thurlkill 9.853
EMBOSS 10.248
Sillero 9.882
Patrickios 10.467
IPC_peptide 10.043
IPC2_peptide 7.849
IPC2.peptide.svr19 7.804
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
2404
128
2055
801.3
90.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.947 ± 1.06
1.581 ± 0.394
5.075 ± 0.467
8.403 ± 0.778
3.245 ± 0.131
5.865 ± 1.465
2.163 ± 0.121
5.74 ± 0.864
7.488 ± 0.408
8.444 ± 1.805
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.121 ± 0.781
3.785 ± 0.678
5.699 ± 0.379
4.201 ± 0.928
5.408 ± 0.154
6.614 ± 0.312
6.406 ± 0.392
6.073 ± 0.982
1.331 ± 0.308
3.411 ± 0.982
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here