Persephonella marina (strain DSM 14350 / EX-H1)

Taxonomy: cellular organisms; Bacteria; Aquificae; Aquificae; Aquificales; Hydrogenothermaceae; Persephonella; Persephonella marina

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2048 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C0QRJ0|C0QRJ0_PERMH Protoglobin domain-containing protein OS=Persephonella marina (strain DSM 14350 / EX-H1) OX=123214 GN=PERMA_1519 PE=4 SV=1
MM1 pKa = 7.61RR2 pKa = 11.84KK3 pKa = 8.96EE4 pKa = 4.15FKK6 pKa = 10.4KK7 pKa = 10.61KK8 pKa = 10.53AILGGSLSLSALLFACGGAGDD29 pKa = 4.14VASDD33 pKa = 3.58SPYY36 pKa = 9.86ATVTGTVTSASSTGSLSTTGIDD58 pKa = 2.89IGNVNLSFIAAVAIDD73 pKa = 3.94NGKK76 pKa = 9.49LVYY79 pKa = 9.44TADD82 pKa = 5.55DD83 pKa = 3.52IDD85 pKa = 4.3NNGNFNLKK93 pKa = 10.22LKK95 pKa = 10.51EE96 pKa = 4.08GLDD99 pKa = 3.54YY100 pKa = 11.57AFILFDD106 pKa = 3.35ISKK109 pKa = 9.9KK110 pKa = 9.99PKK112 pKa = 10.55LIVKK116 pKa = 9.75DD117 pKa = 3.89SNGNAIKK124 pKa = 10.59INGDD128 pKa = 3.12GSVNISLSDD137 pKa = 3.5SDD139 pKa = 4.84GDD141 pKa = 4.59GIPDD145 pKa = 3.67TASVNITGSVSLTKK159 pKa = 10.54DD160 pKa = 3.08SQLDD164 pKa = 3.84DD165 pKa = 4.41NDD167 pKa = 3.5NDD169 pKa = 4.29HH170 pKa = 6.7YY171 pKa = 11.23PDD173 pKa = 4.85GIEE176 pKa = 4.54NINAGGSVNPDD187 pKa = 2.98YY188 pKa = 11.6DD189 pKa = 3.83EE190 pKa = 6.35DD191 pKa = 5.49GDD193 pKa = 4.73GIFDD197 pKa = 5.24GIEE200 pKa = 4.13DD201 pKa = 4.34KK202 pKa = 11.5DD203 pKa = 3.72GDD205 pKa = 4.26GYY207 pKa = 10.71IDD209 pKa = 3.45GHH211 pKa = 6.31EE212 pKa = 4.67DD213 pKa = 3.45SNSNGIPDD221 pKa = 4.07VYY223 pKa = 11.1EE224 pKa = 5.72DD225 pKa = 4.62DD226 pKa = 5.92DD227 pKa = 6.15KK228 pKa = 12.02DD229 pKa = 3.8GLPNYY234 pKa = 10.38LDD236 pKa = 5.29DD237 pKa = 5.29SDD239 pKa = 5.27GDD241 pKa = 4.19GYY243 pKa = 9.96PDD245 pKa = 5.8HH246 pKa = 8.06IDD248 pKa = 3.64EE249 pKa = 5.8DD250 pKa = 4.79DD251 pKa = 3.76SDD253 pKa = 4.27GYY255 pKa = 10.66KK256 pKa = 10.28YY257 pKa = 10.79EE258 pKa = 4.16MKK260 pKa = 10.06GTVSNIDD267 pKa = 3.34TTTGTFVFTYY277 pKa = 10.58NGTDD281 pKa = 3.47YY282 pKa = 11.0NVSVTDD288 pKa = 3.53TTVCEE293 pKa = 4.46INDD296 pKa = 4.03TYY298 pKa = 11.73YY299 pKa = 11.05KK300 pKa = 10.88GADD303 pKa = 3.92CLSHH307 pKa = 6.36LTDD310 pKa = 3.51GAYY313 pKa = 9.36IEE315 pKa = 5.74LKK317 pKa = 10.21TNDD320 pKa = 5.09DD321 pKa = 3.35ISTATDD327 pKa = 3.21ISAVKK332 pKa = 10.39FEE334 pKa = 4.73MEE336 pKa = 3.88DD337 pKa = 2.91EE338 pKa = 4.8GYY340 pKa = 10.41EE341 pKa = 4.04DD342 pKa = 4.62HH343 pKa = 7.56SSDD346 pKa = 3.1SYY348 pKa = 11.32RR349 pKa = 11.84FEE351 pKa = 5.14IYY353 pKa = 10.83GKK355 pKa = 8.4TKK357 pKa = 10.84NIDD360 pKa = 3.38LTNGTFVFDD369 pKa = 3.89WNGTEE374 pKa = 3.78ITVSVDD380 pKa = 3.3TNIKK384 pKa = 10.67CEE386 pKa = 4.08INDD389 pKa = 3.33VYY391 pKa = 11.53YY392 pKa = 11.2YY393 pKa = 10.43GTDD396 pKa = 3.51CLNNLADD403 pKa = 4.05NLCIEE408 pKa = 5.34LKK410 pKa = 10.3TSDD413 pKa = 4.78DD414 pKa = 3.9VYY416 pKa = 11.29NYY418 pKa = 10.55DD419 pKa = 3.14GSAPITAVEE428 pKa = 4.72FEE430 pKa = 4.33TSDD433 pKa = 4.15DD434 pKa = 4.16CGTSYY439 pKa = 11.7

Molecular weight: 47.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C0QQI0|C0QQI0_PERMH Sodium-driven multidrug efflux pump protein OS=Persephonella marina (strain DSM 14350 / EX-H1) OX=123214 GN=PERMA_1144 PE=4 SV=1
MM1 pKa = 8.07DD2 pKa = 3.3NTARR6 pKa = 11.84LDD8 pKa = 3.58YY9 pKa = 10.43PRR11 pKa = 11.84VKK13 pKa = 10.79GGISPAAPGTPKK25 pKa = 10.59GALPSLPPILRR36 pKa = 11.84MTRR39 pKa = 11.84KK40 pKa = 9.71QSMPGYY46 pKa = 8.93SKK48 pKa = 11.05ASRR51 pKa = 11.84GLSVLPRR58 pKa = 11.84VVGILTDD65 pKa = 3.62TTISPGLSSRR75 pKa = 11.84QRR77 pKa = 11.84GDD79 pKa = 2.16RR80 pKa = 11.84WTIHH84 pKa = 6.78AGRR87 pKa = 11.84NLPDD91 pKa = 3.14KK92 pKa = 10.04EE93 pKa = 4.13FRR95 pKa = 11.84YY96 pKa = 10.06HH97 pKa = 5.39RR98 pKa = 11.84TVIVTAAVYY107 pKa = 9.81PGFGSRR113 pKa = 11.84LAPLPLTYY121 pKa = 10.47GHH123 pKa = 7.22WAGFTPHH130 pKa = 6.41TSSFEE135 pKa = 3.91FAQCCVFGKK144 pKa = 10.15QSPPPGFCDD153 pKa = 4.44PLPHH157 pKa = 7.2LNGTACGHH165 pKa = 6.22PLSRR169 pKa = 11.84SYY171 pKa = 11.23GVNLPSSLRR180 pKa = 11.84RR181 pKa = 11.84VTPTALGYY189 pKa = 10.69

Molecular weight: 20.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2048

0

2048

622608

37

5809

304.0

34.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.536 ± 0.062

0.742 ± 0.023

5.699 ± 0.044

8.178 ± 0.077

4.992 ± 0.057

6.49 ± 0.056

1.507 ± 0.025

9.36 ± 0.053

9.185 ± 0.07

9.283 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.999 ± 0.024

4.241 ± 0.061

3.671 ± 0.039

2.328 ± 0.03

4.664 ± 0.044

5.676 ± 0.071

4.562 ± 0.042

6.839 ± 0.044

0.804 ± 0.021

4.245 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski