Xanthomonas citri phage CP2
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 40 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K7ZLE9|K7ZLE9_9CAUD Uncharacterized protein OS=Xanthomonas citri phage CP2 OX=1188795 PE=4 SV=1
MM1 pKa = 7.03 TVSANSRR8 pKa = 11.84 RR9 pKa = 11.84 RR10 pKa = 11.84 EE11 pKa = 3.87 YY12 pKa = 10.53 QGNGVTQVFNGPMAYY27 pKa = 10.1 ARR29 pKa = 11.84 AHH31 pKa = 5.77 VYY33 pKa = 10.96 AFLVKK38 pKa = 9.93 GKK40 pKa = 8.85 TMTLVPNASFDD51 pKa = 3.94 VEE53 pKa = 4.15 KK54 pKa = 10.83 LGYY57 pKa = 10.12 EE58 pKa = 3.77 SGTRR62 pKa = 11.84 VTMHH66 pKa = 6.87 TPPAADD72 pKa = 3.58 EE73 pKa = 4.09 MLLLLRR79 pKa = 11.84 TMPYY83 pKa = 8.38 TQEE86 pKa = 3.69 TDD88 pKa = 2.79 ITNQGAFHH96 pKa = 7.35 AEE98 pKa = 4.19 TIEE101 pKa = 4.04 KK102 pKa = 10.63 GFDD105 pKa = 3.36 ALEE108 pKa = 3.93 MQIQQLVDD116 pKa = 3.25 GTIQLVFEE124 pKa = 5.26 DD125 pKa = 6.01 GEE127 pKa = 4.68 FVWDD131 pKa = 3.88 AKK133 pKa = 10.24 GSRR136 pKa = 11.84 IIRR139 pKa = 11.84 VGDD142 pKa = 3.35 PTGDD146 pKa = 3.15 QDD148 pKa = 4.64 AVNLRR153 pKa = 11.84 TLYY156 pKa = 10.89 LVIEE160 pKa = 4.36 QIQNGGGSVGVSPKK174 pKa = 9.62 YY175 pKa = 10.11 WEE177 pKa = 3.86 ITGDD181 pKa = 4.02 GEE183 pKa = 4.54 DD184 pKa = 4.21 TDD186 pKa = 5.37 FEE188 pKa = 5.0 LPGSDD193 pKa = 3.69 VSDD196 pKa = 3.54 LLFYY200 pKa = 11.12 DD201 pKa = 4.05 VVVEE205 pKa = 4.24 GLSKK209 pKa = 10.69 EE210 pKa = 4.37 PYY212 pKa = 9.9 DD213 pKa = 3.92 EE214 pKa = 4.17 YY215 pKa = 11.18 TIVAASDD222 pKa = 3.6 GSDD225 pKa = 3.29 FAVRR229 pKa = 11.84 FPAPLPNGAEE239 pKa = 4.0 GFVILRR245 pKa = 11.84 GYY247 pKa = 10.62 AKK249 pKa = 10.0 PFTGNTPVQTLRR261 pKa = 11.84 IPIIDD266 pKa = 3.73 VDD268 pKa = 3.65 ATTYY272 pKa = 10.31 TVDD275 pKa = 3.34 KK276 pKa = 10.54 SSEE279 pKa = 3.96 FALLRR284 pKa = 11.84 TTNVNPCLLTLRR296 pKa = 11.84 ANQGDD301 pKa = 4.62 LFDD304 pKa = 4.91 MGDD307 pKa = 3.0 GSYY310 pKa = 11.07 FSVCQRR316 pKa = 11.84 GDD318 pKa = 3.12 GQARR322 pKa = 11.84 IVADD326 pKa = 3.42 TGVTLVLPEE335 pKa = 4.08 GFVPYY340 pKa = 9.63 TRR342 pKa = 11.84 APGSIISAVCEE353 pKa = 4.01 YY354 pKa = 11.34 AEE356 pKa = 4.35 GDD358 pKa = 3.61 TWVLSGDD365 pKa = 4.04 LAQGG369 pKa = 3.38
Molecular weight: 40.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.288
IPC2_protein 4.253
IPC_protein 4.228
Toseland 4.037
ProMoST 4.368
Dawson 4.202
Bjellqvist 4.355
Wikipedia 4.101
Rodwell 4.062
Grimsley 3.948
Solomon 4.202
Lehninger 4.151
Nozaki 4.304
DTASelect 4.52
Thurlkill 4.062
EMBOSS 4.113
Sillero 4.342
Patrickios 3.516
IPC_peptide 4.202
IPC2_peptide 4.329
IPC2.peptide.svr19 4.272
Protein with the highest isoelectric point:
>tr|K7ZJS9|K7ZJS9_9CAUD Uncharacterized protein OS=Xanthomonas citri phage CP2 OX=1188795 PE=4 SV=1
MM1 pKa = 7.56 GARR4 pKa = 11.84 TPSPPYY10 pKa = 8.56 PRR12 pKa = 11.84 MMKK15 pKa = 9.84 RR16 pKa = 11.84 VRR18 pKa = 11.84 VAEE21 pKa = 4.28 SGCWLYY27 pKa = 10.73 TGSKK31 pKa = 10.41 DD32 pKa = 3.14 GGGYY36 pKa = 8.34 GTVSIRR42 pKa = 11.84 RR43 pKa = 11.84 GAAPGKK49 pKa = 9.25 AHH51 pKa = 7.28 RR52 pKa = 11.84 ISYY55 pKa = 7.13 EE56 pKa = 3.83 HH57 pKa = 6.94 HH58 pKa = 6.37 VGAIPVGAMVCHH70 pKa = 6.52 RR71 pKa = 11.84 CDD73 pKa = 3.09 TPACVNPDD81 pKa = 3.16 HH82 pKa = 7.01 LFIGTQGDD90 pKa = 3.59 NMQDD94 pKa = 3.49 CASKK98 pKa = 10.94 GRR100 pKa = 11.84 ISPRR104 pKa = 11.84 SILNLQPGAPGHH116 pKa = 6.6 HH117 pKa = 6.19 GAAPRR122 pKa = 11.84 KK123 pKa = 8.56 QAA125 pKa = 3.65
Molecular weight: 13.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.25
IPC2_protein 9.18
IPC_protein 9.341
Toseland 9.721
ProMoST 9.575
Dawson 10.014
Bjellqvist 9.823
Wikipedia 10.204
Rodwell 10.204
Grimsley 10.087
Solomon 10.072
Lehninger 10.043
Nozaki 9.97
DTASelect 9.75
Thurlkill 9.867
EMBOSS 10.16
Sillero 9.984
Patrickios 7.541
IPC_peptide 10.058
IPC2_peptide 9.121
IPC2.peptide.svr19 7.924
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
40
0
40
12684
104
2478
317.1
34.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.679 ± 0.819
0.922 ± 0.295
6.457 ± 0.335
5.637 ± 0.326
2.996 ± 0.202
8.538 ± 0.369
1.451 ± 0.146
4.06 ± 0.276
3.784 ± 0.269
7.411 ± 0.397
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.294 ± 0.127
3.319 ± 0.233
5.503 ± 0.647
4.857 ± 0.287
6.875 ± 0.38
5.361 ± 0.335
5.905 ± 0.261
7.017 ± 0.301
1.632 ± 0.205
2.302 ± 0.156
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here