Chamaesiphon minutus (strain ATCC 27169 / PCC 6605)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Chamaesiphonaceae; Chamaesiphon; Chamaesiphon minutus

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5815 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K9UP81|K9UP81_CHAP6 Cell shape-determining protein MreB OS=Chamaesiphon minutus (strain ATCC 27169 / PCC 6605) OX=1173020 GN=mreB PE=3 SV=1
MM1 pKa = 7.8IDD3 pKa = 3.21NSANNNSPSRR13 pKa = 11.84LWFAHH18 pKa = 4.59NWEE21 pKa = 4.56SEE23 pKa = 4.62SFFPTQEE30 pKa = 3.7EE31 pKa = 4.6AIAEE35 pKa = 4.19ANSDD39 pKa = 4.26LKK41 pKa = 11.13CCQDD45 pKa = 3.88PDD47 pKa = 3.94DD48 pKa = 5.6GSWADD53 pKa = 3.85YY54 pKa = 11.48VEE56 pKa = 5.35DD57 pKa = 4.01IYY59 pKa = 11.67VGYY62 pKa = 8.51VTHH65 pKa = 5.77QVKK68 pKa = 10.32PNADD72 pKa = 3.77GYY74 pKa = 10.69SLEE77 pKa = 4.25PTLDD81 pKa = 3.36RR82 pKa = 11.84NSPEE86 pKa = 3.59EE87 pKa = 4.01RR88 pKa = 11.84LCQRR92 pKa = 11.84VVATVLAALRR102 pKa = 11.84YY103 pKa = 8.26FQANRR108 pKa = 11.84DD109 pKa = 4.32DD110 pKa = 5.5ILDD113 pKa = 4.12LDD115 pKa = 4.66LPHH118 pKa = 6.8FVNDD122 pKa = 3.91EE123 pKa = 4.15PLSSDD128 pKa = 4.76EE129 pKa = 5.71IDD131 pKa = 4.35DD132 pKa = 4.05LCEE135 pKa = 4.47AICLSEE141 pKa = 4.11VQLL144 pKa = 4.27

Molecular weight: 16.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K9UNM6|K9UNM6_CHAP6 WD40 repeat-containing protein OS=Chamaesiphon minutus (strain ATCC 27169 / PCC 6605) OX=1173020 GN=Cha6605_4861 PE=4 SV=1
MM1 pKa = 7.55TKK3 pKa = 9.13RR4 pKa = 11.84TLGGTNRR11 pKa = 11.84KK12 pKa = 7.61QKK14 pKa = 8.99RR15 pKa = 11.84TSGFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84SRR26 pKa = 11.84TGQKK30 pKa = 9.54VIKK33 pKa = 9.95ARR35 pKa = 11.84RR36 pKa = 11.84ARR38 pKa = 11.84GRR40 pKa = 11.84HH41 pKa = 4.93RR42 pKa = 11.84LSVV45 pKa = 3.19

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5815

0

5815

1743111

25

2788

299.8

33.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.773 ± 0.038

1.048 ± 0.012

5.454 ± 0.027

5.666 ± 0.035

3.736 ± 0.021

6.68 ± 0.039

1.874 ± 0.018

7.069 ± 0.028

4.454 ± 0.031

10.758 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.962 ± 0.016

4.051 ± 0.028

4.753 ± 0.028

4.89 ± 0.022

5.582 ± 0.023

6.51 ± 0.028

5.934 ± 0.031

6.401 ± 0.029

1.419 ± 0.016

2.985 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski