Staphylococcus microti

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Staphylococcaceae; Staphylococcus

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2166 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D6XS18|A0A0D6XS18_9STAP Zinc metalloprotease OS=Staphylococcus microti OX=569857 GN=NCTC13832_00988 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 10.13KK3 pKa = 10.15QSFLVGVVASTLLLTGCNFLTPNNQNNNQSTNGANNQSSNQNNQQNTNGQGSYY56 pKa = 10.04IYY58 pKa = 10.04DD59 pKa = 3.39QNTRR63 pKa = 11.84EE64 pKa = 4.14YY65 pKa = 10.51YY66 pKa = 9.16AQIWLTVRR74 pKa = 11.84DD75 pKa = 4.02NIEE78 pKa = 3.92TFNQGDD84 pKa = 3.62VSYY87 pKa = 10.25TPVDD91 pKa = 3.49VAGTLVNPYY100 pKa = 9.99NADD103 pKa = 3.1ATATYY108 pKa = 10.32PEE110 pKa = 4.37GTITLSASPTAAGSITFKK128 pKa = 10.74DD129 pKa = 3.54NHH131 pKa = 6.98DD132 pKa = 3.79GTITVYY138 pKa = 10.18NAPGHH143 pKa = 5.52FHH145 pKa = 7.65DD146 pKa = 5.94DD147 pKa = 2.76RR148 pKa = 11.84WFEE151 pKa = 4.48DD152 pKa = 3.73DD153 pKa = 3.69FSLRR157 pKa = 11.84EE158 pKa = 3.92SQKK161 pKa = 10.86IIDD164 pKa = 3.83NGYY167 pKa = 7.73TLTIKK172 pKa = 10.45NASQSDD178 pKa = 3.6INYY181 pKa = 7.33VAQYY185 pKa = 8.27ITQSTPQPSPSEE197 pKa = 4.06AYY199 pKa = 9.79EE200 pKa = 4.84GIDD203 pKa = 3.19SGRR206 pKa = 11.84EE207 pKa = 3.74EE208 pKa = 4.37SSSSEE213 pKa = 3.81DD214 pKa = 3.43SEE216 pKa = 4.3ITVTRR221 pKa = 11.84DD222 pKa = 3.09NVIDD226 pKa = 3.92LVEE229 pKa = 4.83AYY231 pKa = 9.86EE232 pKa = 4.95GDD234 pKa = 4.0FLDD237 pKa = 3.78TDD239 pKa = 4.01TYY241 pKa = 10.25TFKK244 pKa = 10.9EE245 pKa = 4.4PEE247 pKa = 4.1KK248 pKa = 10.37MSDD251 pKa = 3.64GSWGFSILDD260 pKa = 3.62KK261 pKa = 10.6EE262 pKa = 4.61TGEE265 pKa = 4.19LAGSYY270 pKa = 10.14IIDD273 pKa = 4.07PDD275 pKa = 3.98GTVTKK280 pKa = 10.37YY281 pKa = 10.63DD282 pKa = 3.31EE283 pKa = 4.96HH284 pKa = 7.2GNPEE288 pKa = 3.79

Molecular weight: 31.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D6XN60|A0A0D6XN60_9STAP ATP-dependent zinc metalloprotease FtsH OS=Staphylococcus microti OX=569857 GN=ftsH PE=3 SV=1
MM1 pKa = 7.38KK2 pKa = 9.94VQRR5 pKa = 11.84IVIQQKK11 pKa = 8.25HH12 pKa = 4.4QKK14 pKa = 10.12ANQLKK19 pKa = 9.59MMRR22 pKa = 11.84QHH24 pKa = 5.78QLNRR28 pKa = 11.84TVNRR32 pKa = 11.84LLNQKK37 pKa = 9.25VRR39 pKa = 11.84VRR41 pKa = 11.84LNQYY45 pKa = 9.73LFQRR49 pKa = 11.84ASKK52 pKa = 10.02LQHH55 pKa = 5.18QKK57 pKa = 10.05VRR59 pKa = 11.84VHH61 pKa = 5.87QLQKK65 pKa = 10.52VKK67 pKa = 10.47KK68 pKa = 7.57HH69 pKa = 4.4QKK71 pKa = 8.77VRR73 pKa = 11.84KK74 pKa = 8.19QRR76 pKa = 11.84LRR78 pKa = 11.84IQKK81 pKa = 7.7QHH83 pKa = 5.19QRR85 pKa = 11.84AKK87 pKa = 9.23RR88 pKa = 11.84QVTVTHH94 pKa = 5.86NHH96 pKa = 4.44QMFPRR101 pKa = 11.84PQTAA105 pKa = 2.73

Molecular weight: 13.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2166

0

2166

640098

37

2944

295.5

33.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.378 ± 0.088

0.625 ± 0.016

5.982 ± 0.08

6.643 ± 0.073

4.312 ± 0.053

6.329 ± 0.052

2.494 ± 0.029

7.724 ± 0.061

6.439 ± 0.06

9.402 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.819 ± 0.03

4.417 ± 0.036

3.486 ± 0.038

4.503 ± 0.044

4.015 ± 0.041

5.523 ± 0.073

6.039 ± 0.041

7.225 ± 0.042

0.761 ± 0.018

3.886 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski