Amino acid dipepetide frequency for Staphylococcus microti

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.182AlaAla: 5.182 ± 0.203
0.575AlaCys: 0.575 ± 0.031
3.851AlaAsp: 3.851 ± 0.166
4.643AlaGlu: 4.643 ± 0.16
3.37AlaPhe: 3.37 ± 0.083
4.624AlaGly: 4.624 ± 0.109
1.895AlaHis: 1.895 ± 0.061
6.18AlaIle: 6.18 ± 0.114
4.648AlaLys: 4.648 ± 0.181
8.064AlaLeu: 8.064 ± 0.142
2.251AlaMet: 2.251 ± 0.07
2.796AlaAsn: 2.796 ± 0.082
2.225AlaPro: 2.225 ± 0.066
3.701AlaGln: 3.701 ± 0.119
2.882AlaArg: 2.882 ± 0.067
4.024AlaSer: 4.024 ± 0.077
4.324AlaThr: 4.324 ± 0.084
5.184AlaVal: 5.184 ± 0.118
0.511AlaTrp: 0.511 ± 0.033
2.846AlaTyr: 2.846 ± 0.069
0.0AlaXaa: 0.0 ± 0.0
Cys
0.397CysAla: 0.397 ± 0.024
0.078CysCys: 0.078 ± 0.012
0.389CysAsp: 0.389 ± 0.026
0.35CysGlu: 0.35 ± 0.025
0.256CysPhe: 0.256 ± 0.021
0.614CysGly: 0.614 ± 0.035
0.195CysHis: 0.195 ± 0.018
0.508CysIle: 0.508 ± 0.024
0.264CysLys: 0.264 ± 0.022
0.558CysLeu: 0.558 ± 0.035
0.162CysMet: 0.162 ± 0.015
0.234CysAsn: 0.234 ± 0.021
0.327CysPro: 0.327 ± 0.024
0.239CysGln: 0.239 ± 0.019
0.256CysArg: 0.256 ± 0.02
0.375CysSer: 0.375 ± 0.026
0.325CysThr: 0.325 ± 0.026
0.43CysVal: 0.43 ± 0.027
0.034CysTrp: 0.034 ± 0.006
0.255CysTyr: 0.255 ± 0.023
0.0CysXaa: 0.0 ± 0.0
Asp
4.615AspAla: 4.615 ± 0.185
0.319AspCys: 0.319 ± 0.022
3.623AspAsp: 3.623 ± 0.091
4.615AspGlu: 4.615 ± 0.093
2.554AspPhe: 2.554 ± 0.07
3.553AspGly: 3.553 ± 0.107
1.276AspHis: 1.276 ± 0.047
4.841AspIle: 4.841 ± 0.095
3.996AspLys: 3.996 ± 0.104
4.866AspLeu: 4.866 ± 0.098
1.703AspMet: 1.703 ± 0.056
2.314AspAsn: 2.314 ± 0.059
1.92AspPro: 1.92 ± 0.109
2.242AspGln: 2.242 ± 0.086
2.147AspArg: 2.147 ± 0.066
3.398AspSer: 3.398 ± 0.542
3.392AspThr: 3.392 ± 0.077
5.043AspVal: 5.043 ± 0.121
0.52AspTrp: 0.52 ± 0.029
2.886AspTyr: 2.886 ± 0.071
0.0AspXaa: 0.0 ± 0.0
Glu
5.973GluAla: 5.973 ± 0.151
0.25GluCys: 0.25 ± 0.022
3.857GluAsp: 3.857 ± 0.099
4.643GluGlu: 4.643 ± 0.139
1.776GluPhe: 1.776 ± 0.051
3.67GluGly: 3.67 ± 0.086
1.647GluHis: 1.647 ± 0.054
4.738GluIle: 4.738 ± 0.098
4.87GluLys: 4.87 ± 0.125
5.559GluLeu: 5.559 ± 0.114
2.184GluMet: 2.184 ± 0.057
3.076GluAsn: 3.076 ± 0.07
1.939GluPro: 1.939 ± 0.069
3.623GluGln: 3.623 ± 0.091
3.445GluArg: 3.445 ± 0.083
3.27GluSer: 3.27 ± 0.104
4.534GluThr: 4.534 ± 0.094
4.959GluVal: 4.959 ± 0.102
0.541GluTrp: 0.541 ± 0.032
1.879GluTyr: 1.879 ± 0.062
0.0GluXaa: 0.0 ± 0.0
Phe
2.765PheAla: 2.765 ± 0.068
0.28PheCys: 0.28 ± 0.018
2.87PheAsp: 2.87 ± 0.074
3.021PheGlu: 3.021 ± 0.087
2.034PhePhe: 2.034 ± 0.076
3.046PheGly: 3.046 ± 0.078
0.886PheHis: 0.886 ± 0.036
3.762PheIle: 3.762 ± 0.114
2.864PheLys: 2.864 ± 0.069
3.77PheLeu: 3.77 ± 0.101
1.123PheMet: 1.123 ± 0.042
2.273PheAsn: 2.273 ± 0.065
1.319PhePro: 1.319 ± 0.046
1.512PheGln: 1.512 ± 0.054
1.378PheArg: 1.378 ± 0.046
2.475PheSer: 2.475 ± 0.063
2.322PheThr: 2.322 ± 0.066
3.409PheVal: 3.409 ± 0.091
0.328PheTrp: 0.328 ± 0.029
1.679PheTyr: 1.679 ± 0.054
0.0PheXaa: 0.0 ± 0.0
Gly
4.715GlyAla: 4.715 ± 0.111
0.487GlyCys: 0.487 ± 0.033
3.317GlyAsp: 3.317 ± 0.071
3.617GlyGlu: 3.617 ± 0.084
3.093GlyPhe: 3.093 ± 0.078
4.299GlyGly: 4.299 ± 0.095
1.629GlyHis: 1.629 ± 0.056
5.301GlyIle: 5.301 ± 0.123
4.093GlyLys: 4.093 ± 0.091
6.132GlyLeu: 6.132 ± 0.12
1.847GlyMet: 1.847 ± 0.056
2.406GlyAsn: 2.406 ± 0.074
1.62GlyPro: 1.62 ± 0.054
2.553GlyGln: 2.553 ± 0.056
2.575GlyArg: 2.575 ± 0.071
3.176GlySer: 3.176 ± 0.07
3.895GlyThr: 3.895 ± 0.104
5.16GlyVal: 5.16 ± 0.106
0.533GlyTrp: 0.533 ± 0.033
2.84GlyTyr: 2.84 ± 0.078
0.0GlyXaa: 0.0 ± 0.0
His
1.767HisAla: 1.767 ± 0.052
0.147HisCys: 0.147 ± 0.015
1.57HisAsp: 1.57 ± 0.05
1.558HisGlu: 1.558 ± 0.057
1.27HisPhe: 1.27 ± 0.048
1.476HisGly: 1.476 ± 0.056
0.875HisHis: 0.875 ± 0.039
2.09HisIle: 2.09 ± 0.061
1.297HisLys: 1.297 ± 0.041
2.301HisLeu: 2.301 ± 0.057
0.672HisMet: 0.672 ± 0.035
1.014HisAsn: 1.014 ± 0.036
1.153HisPro: 1.153 ± 0.041
1.144HisGln: 1.144 ± 0.049
0.995HisArg: 0.995 ± 0.04
1.048HisSer: 1.048 ± 0.036
1.367HisThr: 1.367 ± 0.047
1.77HisVal: 1.77 ± 0.051
0.184HisTrp: 0.184 ± 0.017
1.244HisTyr: 1.244 ± 0.048
0.0HisXaa: 0.0 ± 0.0
Ile
6.44IleAla: 6.44 ± 0.111
0.597IleCys: 0.597 ± 0.033
5.215IleAsp: 5.215 ± 0.096
5.566IleGlu: 5.566 ± 0.101
3.195IlePhe: 3.195 ± 0.098
5.743IleGly: 5.743 ± 0.131
1.809IleHis: 1.809 ± 0.052
5.71IleIle: 5.71 ± 0.141
4.543IleLys: 4.543 ± 0.087
6.591IleLeu: 6.591 ± 0.148
1.826IleMet: 1.826 ± 0.058
3.403IleAsn: 3.403 ± 0.073
2.907IlePro: 2.907 ± 0.078
3.351IleGln: 3.351 ± 0.077
2.723IleArg: 2.723 ± 0.062
4.296IleSer: 4.296 ± 0.107
4.407IleThr: 4.407 ± 0.096
5.893IleVal: 5.893 ± 0.109
0.494IleTrp: 0.494 ± 0.029
2.529IleTyr: 2.529 ± 0.074
0.0IleXaa: 0.0 ± 0.0
Lys
4.949LysAla: 4.949 ± 0.185
0.286LysCys: 0.286 ± 0.023
4.084LysAsp: 4.084 ± 0.118
4.837LysGlu: 4.837 ± 0.105
1.992LysPhe: 1.992 ± 0.062
4.035LysGly: 4.035 ± 0.086
1.609LysHis: 1.609 ± 0.049
3.931LysIle: 3.931 ± 0.082
4.812LysLys: 4.812 ± 0.1
5.398LysLeu: 5.398 ± 0.097
1.964LysMet: 1.964 ± 0.058
3.0LysAsn: 3.0 ± 0.082
2.129LysPro: 2.129 ± 0.063
3.559LysGln: 3.559 ± 0.091
3.215LysArg: 3.215 ± 0.084
3.389LysSer: 3.389 ± 0.074
3.612LysThr: 3.612 ± 0.077
4.731LysVal: 4.731 ± 0.101
0.52LysTrp: 0.52 ± 0.026
2.336LysTyr: 2.336 ± 0.071
0.0LysXaa: 0.0 ± 0.0
Leu
6.935LeuAla: 6.935 ± 0.125
0.625LeuCys: 0.625 ± 0.031
5.282LeuAsp: 5.282 ± 0.117
5.787LeuGlu: 5.787 ± 0.109
4.306LeuPhe: 4.306 ± 0.111
5.963LeuGly: 5.963 ± 0.109
1.926LeuHis: 1.926 ± 0.061
7.325LeuIle: 7.325 ± 0.152
6.48LeuLys: 6.48 ± 0.1
9.111LeuLeu: 9.111 ± 0.183
2.754LeuMet: 2.754 ± 0.087
4.629LeuAsn: 4.629 ± 0.093
3.756LeuPro: 3.756 ± 0.109
3.506LeuGln: 3.506 ± 0.076
3.282LeuArg: 3.282 ± 0.081
6.444LeuSer: 6.444 ± 0.141
6.107LeuThr: 6.107 ± 0.108
6.074LeuVal: 6.074 ± 0.119
0.631LeuTrp: 0.631 ± 0.032
3.092LeuTyr: 3.092 ± 0.084
0.0LeuXaa: 0.0 ± 0.0
Met
2.059MetAla: 2.059 ± 0.064
0.162MetCys: 0.162 ± 0.013
1.428MetAsp: 1.428 ± 0.044
1.323MetGlu: 1.323 ± 0.046
1.067MetPhe: 1.067 ± 0.044
1.615MetGly: 1.615 ± 0.055
0.628MetHis: 0.628 ± 0.026
2.392MetIle: 2.392 ± 0.069
2.17MetLys: 2.17 ± 0.061
2.712MetLeu: 2.712 ± 0.083
1.028MetMet: 1.028 ± 0.046
1.498MetAsn: 1.498 ± 0.045
1.065MetPro: 1.065 ± 0.04
1.109MetGln: 1.109 ± 0.042
1.208MetArg: 1.208 ± 0.042
1.87MetSer: 1.87 ± 0.061
2.156MetThr: 2.156 ± 0.061
1.554MetVal: 1.554 ± 0.051
0.162MetTrp: 0.162 ± 0.017
0.983MetTyr: 0.983 ± 0.039
0.0MetXaa: 0.0 ± 0.0
Asn
3.185AsnAla: 3.185 ± 0.083
0.258AsnCys: 0.258 ± 0.023
2.848AsnAsp: 2.848 ± 0.077
3.223AsnGlu: 3.223 ± 0.073
1.79AsnPhe: 1.79 ± 0.052
3.032AsnGly: 3.032 ± 0.087
1.209AsnHis: 1.209 ± 0.045
3.499AsnIle: 3.499 ± 0.081
3.037AsnLys: 3.037 ± 0.074
3.426AsnLeu: 3.426 ± 0.081
1.214AsnMet: 1.214 ± 0.043
2.081AsnAsn: 2.081 ± 0.076
1.92AsnPro: 1.92 ± 0.054
2.109AsnGln: 2.109 ± 0.07
1.793AsnArg: 1.793 ± 0.054
1.843AsnSer: 1.843 ± 0.059
2.362AsnThr: 2.362 ± 0.066
3.229AsnVal: 3.229 ± 0.073
0.314AsnTrp: 0.314 ± 0.027
1.8AsnTyr: 1.8 ± 0.055
0.0AsnXaa: 0.0 ± 0.0
Pro
2.164ProAla: 2.164 ± 0.09
0.172ProCys: 0.172 ± 0.015
1.942ProAsp: 1.942 ± 0.056
2.815ProGlu: 2.815 ± 0.109
1.784ProPhe: 1.784 ± 0.06
2.001ProGly: 2.001 ± 0.068
0.884ProHis: 0.884 ± 0.034
2.771ProIle: 2.771 ± 0.069
2.315ProLys: 2.315 ± 0.064
3.267ProLeu: 3.267 ± 0.083
0.837ProMet: 0.837 ± 0.043
1.79ProAsn: 1.79 ± 0.057
0.761ProPro: 0.761 ± 0.037
1.292ProGln: 1.292 ± 0.045
1.062ProArg: 1.062 ± 0.041
2.097ProSer: 2.097 ± 0.062
2.382ProThr: 2.382 ± 0.084
2.775ProVal: 2.775 ± 0.079
0.262ProTrp: 0.262 ± 0.024
1.489ProTyr: 1.489 ± 0.046
0.0ProXaa: 0.0 ± 0.0
Gln
3.584GlnAla: 3.584 ± 0.113
0.203GlnCys: 0.203 ± 0.018
2.182GlnAsp: 2.182 ± 0.067
2.456GlnGlu: 2.456 ± 0.077
1.912GlnPhe: 1.912 ± 0.052
2.432GlnGly: 2.432 ± 0.064
1.39GlnHis: 1.39 ± 0.052
2.865GlnIle: 2.865 ± 0.071
2.842GlnLys: 2.842 ± 0.078
4.79GlnLeu: 4.79 ± 0.114
1.283GlnMet: 1.283 ± 0.046
1.981GlnAsn: 1.981 ± 0.058
1.567GlnPro: 1.567 ± 0.052
3.075GlnGln: 3.075 ± 0.103
2.217GlnArg: 2.217 ± 0.06
2.682GlnSer: 2.682 ± 0.082
2.634GlnThr: 2.634 ± 0.081
2.621GlnVal: 2.621 ± 0.068
0.439GlnTrp: 0.439 ± 0.028
1.87GlnTyr: 1.87 ± 0.058
0.0GlnXaa: 0.0 ± 0.0
Arg
2.731ArgAla: 2.731 ± 0.078
0.216ArgCys: 0.216 ± 0.02
2.342ArgAsp: 2.342 ± 0.062
2.796ArgGlu: 2.796 ± 0.069
1.94ArgPhe: 1.94 ± 0.053
2.215ArgGly: 2.215 ± 0.077
1.134ArgHis: 1.134 ± 0.043
3.126ArgIle: 3.126 ± 0.07
2.581ArgLys: 2.581 ± 0.06
3.968ArgLeu: 3.968 ± 0.09
1.201ArgMet: 1.201 ± 0.044
1.656ArgAsn: 1.656 ± 0.046
1.322ArgPro: 1.322 ± 0.058
2.079ArgGln: 2.079 ± 0.056
1.898ArgArg: 1.898 ± 0.058
1.828ArgSer: 1.828 ± 0.053
2.156ArgThr: 2.156 ± 0.061
2.789ArgVal: 2.789 ± 0.08
0.297ArgTrp: 0.297 ± 0.021
1.87ArgTyr: 1.87 ± 0.07
0.0ArgXaa: 0.0 ± 0.0
Ser
3.54SerAla: 3.54 ± 0.084
0.289SerCys: 0.289 ± 0.025
4.185SerAsp: 4.185 ± 0.538
3.668SerGlu: 3.668 ± 0.087
2.667SerPhe: 2.667 ± 0.076
3.709SerGly: 3.709 ± 0.081
1.429SerHis: 1.429 ± 0.047
4.207SerIle: 4.207 ± 0.096
3.378SerLys: 3.378 ± 0.075
5.552SerLeu: 5.552 ± 0.159
1.565SerMet: 1.565 ± 0.055
2.386SerAsn: 2.386 ± 0.068
1.778SerPro: 1.778 ± 0.06
2.253SerGln: 2.253 ± 0.065
2.139SerArg: 2.139 ± 0.055
3.021SerSer: 3.021 ± 0.08
3.062SerThr: 3.062 ± 0.082
3.706SerVal: 3.706 ± 0.089
0.426SerTrp: 0.426 ± 0.028
2.272SerTyr: 2.272 ± 0.056
0.0SerXaa: 0.0 ± 0.0
Thr
4.326ThrAla: 4.326 ± 0.095
0.416ThrCys: 0.416 ± 0.028
3.324ThrAsp: 3.324 ± 0.093
3.734ThrGlu: 3.734 ± 0.084
2.956ThrPhe: 2.956 ± 0.077
3.951ThrGly: 3.951 ± 0.095
1.65ThrHis: 1.65 ± 0.054
4.716ThrIle: 4.716 ± 0.1
3.257ThrLys: 3.257 ± 0.077
6.283ThrLeu: 6.283 ± 0.112
1.45ThrMet: 1.45 ± 0.047
2.387ThrAsn: 2.387 ± 0.065
2.875ThrPro: 2.875 ± 0.093
2.475ThrGln: 2.475 ± 0.064
2.061ThrArg: 2.061 ± 0.059
3.362ThrSer: 3.362 ± 0.102
3.74ThrThr: 3.74 ± 0.095
4.409ThrVal: 4.409 ± 0.097
0.478ThrTrp: 0.478 ± 0.029
2.539ThrTyr: 2.539 ± 0.07
0.0ThrXaa: 0.0 ± 0.0
Val
5.455ValAla: 5.455 ± 0.106
0.562ValCys: 0.562 ± 0.031
4.471ValAsp: 4.471 ± 0.095
4.696ValGlu: 4.696 ± 0.117
2.77ValPhe: 2.77 ± 0.079
4.476ValGly: 4.476 ± 0.098
1.539ValHis: 1.539 ± 0.051
5.854ValIle: 5.854 ± 0.108
4.515ValLys: 4.515 ± 0.092
6.813ValLeu: 6.813 ± 0.129
1.951ValMet: 1.951 ± 0.062
3.264ValAsn: 3.264 ± 0.09
2.729ValPro: 2.729 ± 0.073
2.712ValGln: 2.712 ± 0.069
2.687ValArg: 2.687 ± 0.07
4.365ValSer: 4.365 ± 0.087
4.907ValThr: 4.907 ± 0.093
5.513ValVal: 5.513 ± 0.114
0.514ValTrp: 0.514 ± 0.032
2.454ValTyr: 2.454 ± 0.069
0.0ValXaa: 0.0 ± 0.0
Trp
0.406TrpAla: 0.406 ± 0.027
0.048TrpCys: 0.048 ± 0.009
0.372TrpAsp: 0.372 ± 0.026
0.345TrpGlu: 0.345 ± 0.025
0.442TrpPhe: 0.442 ± 0.027
0.458TrpGly: 0.458 ± 0.033
0.23TrpHis: 0.23 ± 0.017
0.644TrpIle: 0.644 ± 0.036
0.348TrpLys: 0.348 ± 0.027
1.058TrpLeu: 1.058 ± 0.046
0.241TrpMet: 0.241 ± 0.017
0.298TrpAsn: 0.298 ± 0.021
0.198TrpPro: 0.198 ± 0.018
0.409TrpGln: 0.409 ± 0.025
0.336TrpArg: 0.336 ± 0.023
0.45TrpSer: 0.45 ± 0.026
0.458TrpThr: 0.458 ± 0.023
0.464TrpVal: 0.464 ± 0.025
0.086TrpTrp: 0.086 ± 0.012
0.323TrpTyr: 0.323 ± 0.027
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.59TyrAla: 2.59 ± 0.065
0.277TyrCys: 0.277 ± 0.022
2.67TyrAsp: 2.67 ± 0.074
2.737TyrGlu: 2.737 ± 0.065
1.94TyrPhe: 1.94 ± 0.062
2.375TyrGly: 2.375 ± 0.061
1.1TyrHis: 1.1 ± 0.043
2.776TyrIle: 2.776 ± 0.079
2.076TyrLys: 2.076 ± 0.054
3.796TyrLeu: 3.796 ± 0.098
0.933TyrMet: 0.933 ± 0.038
1.689TyrAsn: 1.689 ± 0.055
1.354TyrPro: 1.354 ± 0.043
2.084TyrGln: 2.084 ± 0.064
1.847TyrArg: 1.847 ± 0.051
1.818TyrSer: 1.818 ± 0.059
2.247TyrThr: 2.247 ± 0.059
2.537TyrVal: 2.537 ± 0.063
0.339TyrTrp: 0.339 ± 0.022
1.672TyrTyr: 1.672 ± 0.067
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2166 proteins (640098 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski