Red clover powdery mildew-associated totivirus 7

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Totiviridae; unclassified Totiviridae

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0S3Q2C6|A0A0S3Q2C6_9VIRU RNA-directed RNA polymerase OS=Red clover powdery mildew-associated totivirus 7 OX=1714368 GN=RdRp PE=3 SV=1
MM1 pKa = 7.04NTFSEE6 pKa = 4.82YY7 pKa = 10.55INAFASIDD15 pKa = 3.66ALPAGEE21 pKa = 3.94FALRR25 pKa = 11.84TKK27 pKa = 10.81LRR29 pKa = 11.84LDD31 pKa = 3.5CSGLGKK37 pKa = 9.64PEE39 pKa = 4.03KK40 pKa = 10.54KK41 pKa = 10.23IGDD44 pKa = 3.54TDD46 pKa = 3.62EE47 pKa = 4.85ARR49 pKa = 11.84VTGDD53 pKa = 2.89GHH55 pKa = 7.43VIGDD59 pKa = 3.61TTLPSGEE66 pKa = 4.24TYY68 pKa = 8.77QARR71 pKa = 11.84RR72 pKa = 11.84EE73 pKa = 4.03TAVLSKK79 pKa = 10.16ATVYY83 pKa = 10.73GPQAVVRR90 pKa = 11.84LSGTMNSLSGLAVGALTDD108 pKa = 3.54SGEE111 pKa = 4.19INYY114 pKa = 10.0AWLRR118 pKa = 11.84SEE120 pKa = 4.07FRR122 pKa = 11.84LVNPSVDD129 pKa = 3.42LKK131 pKa = 10.55EE132 pKa = 4.6ARR134 pKa = 11.84TNQLLTAWLEE144 pKa = 3.72RR145 pKa = 11.84GIYY148 pKa = 10.5DD149 pKa = 3.47NAIPLLVKK157 pKa = 10.53LLQKK161 pKa = 10.68LRR163 pKa = 11.84IAQIYY168 pKa = 8.39EE169 pKa = 3.66LSDD172 pKa = 3.32YY173 pKa = 11.0SNEE176 pKa = 3.84VFRR179 pKa = 11.84FGSWFARR186 pKa = 11.84THH188 pKa = 4.23YY189 pKa = 9.05TKK191 pKa = 10.5RR192 pKa = 11.84DD193 pKa = 3.33AALATAISNYY203 pKa = 7.55MRR205 pKa = 11.84NVNTAQRR212 pKa = 11.84IGVCLTDD219 pKa = 3.18LHH221 pKa = 6.16EE222 pKa = 4.75NEE224 pKa = 4.49WVDD227 pKa = 5.92KK228 pKa = 10.69VGEE231 pKa = 4.24WLDD234 pKa = 3.69SQPPPFMEE242 pKa = 4.85GDD244 pKa = 3.33NGEE247 pKa = 4.93KK248 pKa = 9.37IDD250 pKa = 4.24NPKK253 pKa = 10.56CGASPLEE260 pKa = 4.03LPMAVMTCHH269 pKa = 6.58TYY271 pKa = 11.28DD272 pKa = 4.91DD273 pKa = 4.36GHH275 pKa = 6.83SSSGSTFMSEE285 pKa = 3.85SGFVNGRR292 pKa = 11.84FKK294 pKa = 11.48VPVYY298 pKa = 9.55MNKK301 pKa = 9.01IRR303 pKa = 11.84CTEE306 pKa = 4.08TVVKK310 pKa = 10.33NVNYY314 pKa = 9.85YY315 pKa = 10.15EE316 pKa = 4.88CNDD319 pKa = 3.55DD320 pKa = 3.96AAVKK324 pKa = 10.16RR325 pKa = 11.84LVGTEE330 pKa = 3.31HH331 pKa = 7.13HH332 pKa = 7.02INVSGLTAKK341 pKa = 10.15EE342 pKa = 3.57LSLLNFCLSDD352 pKa = 3.52NVRR355 pKa = 11.84RR356 pKa = 11.84TPLLVDD362 pKa = 2.96QVIDD366 pKa = 4.11FKK368 pKa = 11.44LRR370 pKa = 11.84EE371 pKa = 4.18GSVALYY377 pKa = 10.56NAVEE381 pKa = 4.08MQDD384 pKa = 3.48FEE386 pKa = 4.42EE387 pKa = 4.69ARR389 pKa = 11.84FSAKK393 pKa = 10.04EE394 pKa = 3.89MLVLTQKK401 pKa = 10.7LVRR404 pKa = 11.84NHH406 pKa = 5.69NWYY409 pKa = 10.0EE410 pKa = 3.98DD411 pKa = 3.66LRR413 pKa = 11.84SAILFQRR420 pKa = 11.84YY421 pKa = 6.95WLAQPSTEE429 pKa = 4.02TVEE432 pKa = 4.28SHH434 pKa = 4.95WWVNMQRR441 pKa = 11.84VANLPKK447 pKa = 10.36LGLMRR452 pKa = 11.84AAIPQLVSTVGAQISAEE469 pKa = 4.3GLNSMQIIGKK479 pKa = 8.47MDD481 pKa = 3.77LSEE484 pKa = 4.24FVNSVFLNTVWYY496 pKa = 7.97WGEE499 pKa = 3.61YY500 pKa = 8.53LAIYY504 pKa = 8.94SARR507 pKa = 11.84NNEE510 pKa = 3.89HH511 pKa = 6.72LVNKK515 pKa = 8.17MRR517 pKa = 11.84SAIVDD522 pKa = 3.43NVRR525 pKa = 11.84EE526 pKa = 4.12EE527 pKa = 4.2DD528 pKa = 3.4RR529 pKa = 11.84HH530 pKa = 5.76VVLPSAVLGKK540 pKa = 9.96SVPRR544 pKa = 11.84SACKK548 pKa = 10.15LVFVAIAGGVRR559 pKa = 11.84SQGSRR564 pKa = 11.84VTAFGKK570 pKa = 10.12IDD572 pKa = 3.69IPNLDD577 pKa = 3.35EE578 pKa = 5.34RR579 pKa = 11.84GYY581 pKa = 10.78SVNGTDD587 pKa = 2.99VCANCVVEE595 pKa = 4.68PGCLGLVTGLAGTLIRR611 pKa = 11.84NTPYY615 pKa = 10.89SSAFSVNSSALVRR628 pKa = 11.84AWKK631 pKa = 9.98GVRR634 pKa = 11.84VSYY637 pKa = 10.4NWHH640 pKa = 6.9DD641 pKa = 3.34LWALCVVARR650 pKa = 11.84WQGYY654 pKa = 8.34DD655 pKa = 3.2VVYY658 pKa = 8.62RR659 pKa = 11.84TSVSSVRR666 pKa = 11.84RR667 pKa = 11.84YY668 pKa = 9.96RR669 pKa = 11.84SYY671 pKa = 10.49AANMVGIAAPPVKK684 pKa = 9.72MSEE687 pKa = 3.93ATGVGRR693 pKa = 11.84FVVDD697 pKa = 4.12GVLEE701 pKa = 4.27RR702 pKa = 11.84GNVFGSDD709 pKa = 2.86IASQHH714 pKa = 5.65SMLRR718 pKa = 11.84SFTWHH723 pKa = 6.82RR724 pKa = 11.84SQSVVLAEE732 pKa = 4.01PKK734 pKa = 10.26INAFEE739 pKa = 4.17ALAEE743 pKa = 4.15EE744 pKa = 5.18VIHH747 pKa = 7.6IPIEE751 pKa = 3.94HH752 pKa = 7.1YY753 pKa = 9.75ITVNPGATNYY763 pKa = 10.08VGYY766 pKa = 10.4ILADD770 pKa = 3.77YY771 pKa = 11.21DD772 pKa = 4.2HH773 pKa = 7.52DD774 pKa = 3.92TSDD777 pKa = 3.62FRR779 pKa = 11.84VEE781 pKa = 4.2EE782 pKa = 4.18MRR784 pKa = 11.84TAIPFATDD792 pKa = 3.18SNNLGLRR799 pKa = 11.84EE800 pKa = 4.15TVLEE804 pKa = 4.02EE805 pKa = 4.44AEE807 pKa = 4.37PDD809 pKa = 3.61VEE811 pKa = 4.37

Molecular weight:
90.19 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0S3Q2C6|A0A0S3Q2C6_9VIRU RNA-directed RNA polymerase OS=Red clover powdery mildew-associated totivirus 7 OX=1714368 GN=RdRp PE=3 SV=1
MM1 pKa = 7.59SSIPLALSQDD11 pKa = 3.62SRR13 pKa = 11.84GLAAGDD19 pKa = 3.5RR20 pKa = 11.84KK21 pKa = 10.02RR22 pKa = 11.84ASWLLFDD29 pKa = 5.31VITTRR34 pKa = 11.84RR35 pKa = 11.84INGPWVFATGAGAVPTFAIYY55 pKa = 10.01FKK57 pKa = 9.43TLKK60 pKa = 10.63LSALYY65 pKa = 10.04ISNKK69 pKa = 8.87TPLLGQYY76 pKa = 9.96RR77 pKa = 11.84DD78 pKa = 3.23VLMRR82 pKa = 11.84FSRR85 pKa = 11.84IQYY88 pKa = 9.56GPEE91 pKa = 3.69LFPYY95 pKa = 9.03GTVTEE100 pKa = 4.06RR101 pKa = 11.84DD102 pKa = 3.19IMSSLFRR109 pKa = 11.84VTARR113 pKa = 11.84SEE115 pKa = 4.57RR116 pKa = 11.84YY117 pKa = 9.79DD118 pKa = 3.51NNAFPLVRR126 pKa = 11.84QVLKK130 pKa = 10.92GEE132 pKa = 4.16RR133 pKa = 11.84QLEE136 pKa = 4.26HH137 pKa = 6.95ASVSRR142 pKa = 11.84DD143 pKa = 3.31HH144 pKa = 6.76LRR146 pKa = 11.84HH147 pKa = 4.56VTPTEE152 pKa = 4.05LYY154 pKa = 10.09AAYY157 pKa = 10.1GDD159 pKa = 4.24EE160 pKa = 4.95LLDD163 pKa = 3.59STGVCRR169 pKa = 11.84MLEE172 pKa = 3.88RR173 pKa = 11.84LLRR176 pKa = 11.84IPTSRR181 pKa = 11.84DD182 pKa = 2.73GVYY185 pKa = 10.34EE186 pKa = 4.1SAFVGLLLWARR197 pKa = 11.84LIPADD202 pKa = 3.79LRR204 pKa = 11.84DD205 pKa = 3.79WVHH208 pKa = 6.61SSNMWLHH215 pKa = 6.53SYY217 pKa = 10.91VDD219 pKa = 3.94LPDD222 pKa = 3.78FFTRR226 pKa = 11.84VKK228 pKa = 10.63SEE230 pKa = 3.8YY231 pKa = 8.36TLKK234 pKa = 10.91FKK236 pKa = 10.81ALQNNLSVNLTPLFEE251 pKa = 5.55LEE253 pKa = 4.22VLVNRR258 pKa = 11.84GMGTMDD264 pKa = 3.42WEE266 pKa = 4.34SEE268 pKa = 4.09KK269 pKa = 11.21ANRR272 pKa = 11.84VAPNLADD279 pKa = 3.99FSEE282 pKa = 4.52SQVYY286 pKa = 10.26DD287 pKa = 3.42VVKK290 pKa = 10.76RR291 pKa = 11.84ILEE294 pKa = 4.0EE295 pKa = 3.95SRR297 pKa = 11.84VAGGRR302 pKa = 11.84PARR305 pKa = 11.84IEE307 pKa = 3.89WDD309 pKa = 3.49RR310 pKa = 11.84HH311 pKa = 3.5WAEE314 pKa = 3.55RR315 pKa = 11.84WLWAPTGTFHH325 pKa = 6.99SQYY328 pKa = 11.4DD329 pKa = 3.48EE330 pKa = 4.28DD331 pKa = 4.38QEE333 pKa = 4.68FKK335 pKa = 11.03AGCRR339 pKa = 11.84TLNSKK344 pKa = 10.16FYY346 pKa = 10.53ALSRR350 pKa = 11.84MPDD353 pKa = 2.83VKK355 pKa = 11.04YY356 pKa = 9.67EE357 pKa = 3.99HH358 pKa = 7.09FYY360 pKa = 11.23NRR362 pKa = 11.84PPTCKK367 pKa = 9.8ARR369 pKa = 11.84ASVKK373 pKa = 10.32YY374 pKa = 9.53EE375 pKa = 3.5WGKK378 pKa = 9.81QRR380 pKa = 11.84AIYY383 pKa = 9.83GVDD386 pKa = 3.11VTNFIISGFAFKK398 pKa = 10.73GCEE401 pKa = 3.63EE402 pKa = 5.3AISKK406 pKa = 9.95IYY408 pKa = 10.05PIGLEE413 pKa = 3.82AKK415 pKa = 8.38EE416 pKa = 4.23EE417 pKa = 3.99KK418 pKa = 10.31VAAHH422 pKa = 5.23VRR424 pKa = 11.84EE425 pKa = 4.07VLKK428 pKa = 11.07DD429 pKa = 3.5GVPFCFDD436 pKa = 3.42FEE438 pKa = 5.02DD439 pKa = 4.7FNSQHH444 pKa = 6.43SLGSMRR450 pKa = 11.84AVMLAYY456 pKa = 9.87KK457 pKa = 9.23NTFEE461 pKa = 5.52HH462 pKa = 7.68DD463 pKa = 3.18ITPEE467 pKa = 3.74QLRR470 pKa = 11.84GIDD473 pKa = 3.45WMIEE477 pKa = 4.09SLRR480 pKa = 11.84NTQVVSPAGEE490 pKa = 3.79FSIVDD495 pKa = 3.58TLMSGSRR502 pKa = 11.84LTTFMNTVLNDD513 pKa = 3.42AYY515 pKa = 11.26VRR517 pKa = 11.84LLWRR521 pKa = 11.84GRR523 pKa = 11.84VTSTLHH529 pKa = 6.69NGDD532 pKa = 4.34DD533 pKa = 3.54VLAAVTTYY541 pKa = 10.79EE542 pKa = 4.49QVQQLMAGAIDD553 pKa = 3.67HH554 pKa = 6.14NVRR557 pKa = 11.84FQMSKK562 pKa = 9.94CHH564 pKa = 5.89LASVAEE570 pKa = 4.23FLRR573 pKa = 11.84VDD575 pKa = 3.65HH576 pKa = 6.27MAGTGTQYY584 pKa = 11.19LARR587 pKa = 11.84ACATLVHH594 pKa = 6.67GPTDD598 pKa = 3.64ALDD601 pKa = 3.9PRR603 pKa = 11.84DD604 pKa = 3.83PVQQLSAMRR613 pKa = 11.84VRR615 pKa = 11.84AEE617 pKa = 3.54EE618 pKa = 4.48AIARR622 pKa = 11.84GAARR626 pKa = 11.84AEE628 pKa = 4.24VEE630 pKa = 4.1KK631 pKa = 10.13LTTVLRR637 pKa = 11.84EE638 pKa = 3.98SVEE641 pKa = 3.8RR642 pKa = 11.84RR643 pKa = 11.84FNIGFDD649 pKa = 3.34TRR651 pKa = 11.84ILEE654 pKa = 4.3RR655 pKa = 11.84VHH657 pKa = 6.22VSRR660 pKa = 11.84GGYY663 pKa = 9.08SEE665 pKa = 5.13VVDD668 pKa = 4.28EE669 pKa = 4.41EE670 pKa = 4.57TLSKK674 pKa = 10.72EE675 pKa = 4.0VTKK678 pKa = 11.25CEE680 pKa = 4.11VTVNSYY686 pKa = 10.71GDD688 pKa = 3.53KK689 pKa = 10.94AVVDD693 pKa = 3.64MTKK696 pKa = 10.46RR697 pKa = 11.84FPGVDD702 pKa = 2.97AYY704 pKa = 11.03ARR706 pKa = 11.84LVTKK710 pKa = 10.37KK711 pKa = 10.76YY712 pKa = 10.69GIEE715 pKa = 3.83QEE717 pKa = 4.3YY718 pKa = 7.51QTIRR722 pKa = 11.84GNMKK726 pKa = 10.04SAIGRR731 pKa = 11.84AAIEE735 pKa = 4.12GGIGVDD741 pKa = 3.76VRR743 pKa = 11.84DD744 pKa = 3.79VTPDD748 pKa = 3.27HH749 pKa = 6.49LAVLAAHH756 pKa = 6.59KK757 pKa = 10.03RR758 pKa = 11.84KK759 pKa = 10.02AYY761 pKa = 9.3SHH763 pKa = 6.19VRR765 pKa = 11.84LSLKK769 pKa = 10.9ANIARR774 pKa = 11.84AFGIPILLGAEE785 pKa = 4.05YY786 pKa = 9.74GQQYY790 pKa = 10.73NVLLYY795 pKa = 11.11GSVDD799 pKa = 3.04KK800 pKa = 11.43LKK802 pKa = 10.9ALRR805 pKa = 11.84AWFF808 pKa = 3.82

Molecular weight:
91.46 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1619

808

811

809.5

90.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.771 ± 0.174

1.235 ± 0.161

5.312 ± 0.169

6.485 ± 0.033

3.83 ± 0.33

6.485 ± 0.114

2.285 ± 0.125

4.2 ± 0.076

4.015 ± 0.29

9.265 ± 0.174

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.347 ± 0.003

4.509 ± 0.849

3.521 ± 0.036

2.718 ± 0.166

7.288 ± 0.66

7.103 ± 0.439

5.683 ± 0.088

9.265 ± 0.477

1.853 ± 0.079

3.83 ± 0.086

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski