Red clover powdery mildew-associated totivirus 7
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S3Q2C6|A0A0S3Q2C6_9VIRU RNA-directed RNA polymerase OS=Red clover powdery mildew-associated totivirus 7 OX=1714368 GN=RdRp PE=3 SV=1
MM1 pKa = 7.04 NTFSEE6 pKa = 4.82 YY7 pKa = 10.55 INAFASIDD15 pKa = 3.66 ALPAGEE21 pKa = 3.94 FALRR25 pKa = 11.84 TKK27 pKa = 10.81 LRR29 pKa = 11.84 LDD31 pKa = 3.5 CSGLGKK37 pKa = 9.64 PEE39 pKa = 4.03 KK40 pKa = 10.54 KK41 pKa = 10.23 IGDD44 pKa = 3.54 TDD46 pKa = 3.62 EE47 pKa = 4.85 ARR49 pKa = 11.84 VTGDD53 pKa = 2.89 GHH55 pKa = 7.43 VIGDD59 pKa = 3.61 TTLPSGEE66 pKa = 4.24 TYY68 pKa = 8.77 QARR71 pKa = 11.84 RR72 pKa = 11.84 EE73 pKa = 4.03 TAVLSKK79 pKa = 10.16 ATVYY83 pKa = 10.73 GPQAVVRR90 pKa = 11.84 LSGTMNSLSGLAVGALTDD108 pKa = 3.54 SGEE111 pKa = 4.19 INYY114 pKa = 10.0 AWLRR118 pKa = 11.84 SEE120 pKa = 4.07 FRR122 pKa = 11.84 LVNPSVDD129 pKa = 3.42 LKK131 pKa = 10.55 EE132 pKa = 4.6 ARR134 pKa = 11.84 TNQLLTAWLEE144 pKa = 3.72 RR145 pKa = 11.84 GIYY148 pKa = 10.5 DD149 pKa = 3.47 NAIPLLVKK157 pKa = 10.53 LLQKK161 pKa = 10.68 LRR163 pKa = 11.84 IAQIYY168 pKa = 8.39 EE169 pKa = 3.66 LSDD172 pKa = 3.32 YY173 pKa = 11.0 SNEE176 pKa = 3.84 VFRR179 pKa = 11.84 FGSWFARR186 pKa = 11.84 THH188 pKa = 4.23 YY189 pKa = 9.05 TKK191 pKa = 10.5 RR192 pKa = 11.84 DD193 pKa = 3.33 AALATAISNYY203 pKa = 7.55 MRR205 pKa = 11.84 NVNTAQRR212 pKa = 11.84 IGVCLTDD219 pKa = 3.18 LHH221 pKa = 6.16 EE222 pKa = 4.75 NEE224 pKa = 4.49 WVDD227 pKa = 5.92 KK228 pKa = 10.69 VGEE231 pKa = 4.24 WLDD234 pKa = 3.69 SQPPPFMEE242 pKa = 4.85 GDD244 pKa = 3.33 NGEE247 pKa = 4.93 KK248 pKa = 9.37 IDD250 pKa = 4.24 NPKK253 pKa = 10.56 CGASPLEE260 pKa = 4.03 LPMAVMTCHH269 pKa = 6.58 TYY271 pKa = 11.28 DD272 pKa = 4.91 DD273 pKa = 4.36 GHH275 pKa = 6.83 SSSGSTFMSEE285 pKa = 3.85 SGFVNGRR292 pKa = 11.84 FKK294 pKa = 11.48 VPVYY298 pKa = 9.55 MNKK301 pKa = 9.01 IRR303 pKa = 11.84 CTEE306 pKa = 4.08 TVVKK310 pKa = 10.33 NVNYY314 pKa = 9.85 YY315 pKa = 10.15 EE316 pKa = 4.88 CNDD319 pKa = 3.55 DD320 pKa = 3.96 AAVKK324 pKa = 10.16 RR325 pKa = 11.84 LVGTEE330 pKa = 3.31 HH331 pKa = 7.13 HH332 pKa = 7.02 INVSGLTAKK341 pKa = 10.15 EE342 pKa = 3.57 LSLLNFCLSDD352 pKa = 3.52 NVRR355 pKa = 11.84 RR356 pKa = 11.84 TPLLVDD362 pKa = 2.96 QVIDD366 pKa = 4.11 FKK368 pKa = 11.44 LRR370 pKa = 11.84 EE371 pKa = 4.18 GSVALYY377 pKa = 10.56 NAVEE381 pKa = 4.08 MQDD384 pKa = 3.48 FEE386 pKa = 4.42 EE387 pKa = 4.69 ARR389 pKa = 11.84 FSAKK393 pKa = 10.04 EE394 pKa = 3.89 MLVLTQKK401 pKa = 10.7 LVRR404 pKa = 11.84 NHH406 pKa = 5.69 NWYY409 pKa = 10.0 EE410 pKa = 3.98 DD411 pKa = 3.66 LRR413 pKa = 11.84 SAILFQRR420 pKa = 11.84 YY421 pKa = 6.95 WLAQPSTEE429 pKa = 4.02 TVEE432 pKa = 4.28 SHH434 pKa = 4.95 WWVNMQRR441 pKa = 11.84 VANLPKK447 pKa = 10.36 LGLMRR452 pKa = 11.84 AAIPQLVSTVGAQISAEE469 pKa = 4.3 GLNSMQIIGKK479 pKa = 8.47 MDD481 pKa = 3.77 LSEE484 pKa = 4.24 FVNSVFLNTVWYY496 pKa = 7.97 WGEE499 pKa = 3.61 YY500 pKa = 8.53 LAIYY504 pKa = 8.94 SARR507 pKa = 11.84 NNEE510 pKa = 3.89 HH511 pKa = 6.72 LVNKK515 pKa = 8.17 MRR517 pKa = 11.84 SAIVDD522 pKa = 3.43 NVRR525 pKa = 11.84 EE526 pKa = 4.12 EE527 pKa = 4.2 DD528 pKa = 3.4 RR529 pKa = 11.84 HH530 pKa = 5.76 VVLPSAVLGKK540 pKa = 9.96 SVPRR544 pKa = 11.84 SACKK548 pKa = 10.15 LVFVAIAGGVRR559 pKa = 11.84 SQGSRR564 pKa = 11.84 VTAFGKK570 pKa = 10.12 IDD572 pKa = 3.69 IPNLDD577 pKa = 3.35 EE578 pKa = 5.34 RR579 pKa = 11.84 GYY581 pKa = 10.78 SVNGTDD587 pKa = 2.99 VCANCVVEE595 pKa = 4.68 PGCLGLVTGLAGTLIRR611 pKa = 11.84 NTPYY615 pKa = 10.89 SSAFSVNSSALVRR628 pKa = 11.84 AWKK631 pKa = 9.98 GVRR634 pKa = 11.84 VSYY637 pKa = 10.4 NWHH640 pKa = 6.9 DD641 pKa = 3.34 LWALCVVARR650 pKa = 11.84 WQGYY654 pKa = 8.34 DD655 pKa = 3.2 VVYY658 pKa = 8.62 RR659 pKa = 11.84 TSVSSVRR666 pKa = 11.84 RR667 pKa = 11.84 YY668 pKa = 9.96 RR669 pKa = 11.84 SYY671 pKa = 10.49 AANMVGIAAPPVKK684 pKa = 9.72 MSEE687 pKa = 3.93 ATGVGRR693 pKa = 11.84 FVVDD697 pKa = 4.12 GVLEE701 pKa = 4.27 RR702 pKa = 11.84 GNVFGSDD709 pKa = 2.86 IASQHH714 pKa = 5.65 SMLRR718 pKa = 11.84 SFTWHH723 pKa = 6.82 RR724 pKa = 11.84 SQSVVLAEE732 pKa = 4.01 PKK734 pKa = 10.26 INAFEE739 pKa = 4.17 ALAEE743 pKa = 4.15 EE744 pKa = 5.18 VIHH747 pKa = 7.6 IPIEE751 pKa = 3.94 HH752 pKa = 7.1 YY753 pKa = 9.75 ITVNPGATNYY763 pKa = 10.08 VGYY766 pKa = 10.4 ILADD770 pKa = 3.77 YY771 pKa = 11.21 DD772 pKa = 4.2 HH773 pKa = 7.52 DD774 pKa = 3.92 TSDD777 pKa = 3.62 FRR779 pKa = 11.84 VEE781 pKa = 4.2 EE782 pKa = 4.18 MRR784 pKa = 11.84 TAIPFATDD792 pKa = 3.18 SNNLGLRR799 pKa = 11.84 EE800 pKa = 4.15 TVLEE804 pKa = 4.02 EE805 pKa = 4.44 AEE807 pKa = 4.37 PDD809 pKa = 3.61 VEE811 pKa = 4.37
Molecular weight: 90.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.499
IPC2_protein 5.512
IPC_protein 5.537
Toseland 5.804
ProMoST 5.741
Dawson 5.703
Bjellqvist 5.753
Wikipedia 5.664
Rodwell 5.664
Grimsley 5.906
Solomon 5.703
Lehninger 5.677
Nozaki 5.919
DTASelect 6.097
Thurlkill 6.033
EMBOSS 5.982
Sillero 6.008
Patrickios 4.037
IPC_peptide 5.715
IPC2_peptide 6.02
IPC2.peptide.svr19 5.982
Protein with the highest isoelectric point:
>tr|A0A0S3Q2C6|A0A0S3Q2C6_9VIRU RNA-directed RNA polymerase OS=Red clover powdery mildew-associated totivirus 7 OX=1714368 GN=RdRp PE=3 SV=1
MM1 pKa = 7.59 SSIPLALSQDD11 pKa = 3.62 SRR13 pKa = 11.84 GLAAGDD19 pKa = 3.5 RR20 pKa = 11.84 KK21 pKa = 10.02 RR22 pKa = 11.84 ASWLLFDD29 pKa = 5.31 VITTRR34 pKa = 11.84 RR35 pKa = 11.84 INGPWVFATGAGAVPTFAIYY55 pKa = 10.01 FKK57 pKa = 9.43 TLKK60 pKa = 10.63 LSALYY65 pKa = 10.04 ISNKK69 pKa = 8.87 TPLLGQYY76 pKa = 9.96 RR77 pKa = 11.84 DD78 pKa = 3.23 VLMRR82 pKa = 11.84 FSRR85 pKa = 11.84 IQYY88 pKa = 9.56 GPEE91 pKa = 3.69 LFPYY95 pKa = 9.03 GTVTEE100 pKa = 4.06 RR101 pKa = 11.84 DD102 pKa = 3.19 IMSSLFRR109 pKa = 11.84 VTARR113 pKa = 11.84 SEE115 pKa = 4.57 RR116 pKa = 11.84 YY117 pKa = 9.79 DD118 pKa = 3.51 NNAFPLVRR126 pKa = 11.84 QVLKK130 pKa = 10.92 GEE132 pKa = 4.16 RR133 pKa = 11.84 QLEE136 pKa = 4.26 HH137 pKa = 6.95 ASVSRR142 pKa = 11.84 DD143 pKa = 3.31 HH144 pKa = 6.76 LRR146 pKa = 11.84 HH147 pKa = 4.56 VTPTEE152 pKa = 4.05 LYY154 pKa = 10.09 AAYY157 pKa = 10.1 GDD159 pKa = 4.24 EE160 pKa = 4.95 LLDD163 pKa = 3.59 STGVCRR169 pKa = 11.84 MLEE172 pKa = 3.88 RR173 pKa = 11.84 LLRR176 pKa = 11.84 IPTSRR181 pKa = 11.84 DD182 pKa = 2.73 GVYY185 pKa = 10.34 EE186 pKa = 4.1 SAFVGLLLWARR197 pKa = 11.84 LIPADD202 pKa = 3.79 LRR204 pKa = 11.84 DD205 pKa = 3.79 WVHH208 pKa = 6.61 SSNMWLHH215 pKa = 6.53 SYY217 pKa = 10.91 VDD219 pKa = 3.94 LPDD222 pKa = 3.78 FFTRR226 pKa = 11.84 VKK228 pKa = 10.63 SEE230 pKa = 3.8 YY231 pKa = 8.36 TLKK234 pKa = 10.91 FKK236 pKa = 10.81 ALQNNLSVNLTPLFEE251 pKa = 5.55 LEE253 pKa = 4.22 VLVNRR258 pKa = 11.84 GMGTMDD264 pKa = 3.42 WEE266 pKa = 4.34 SEE268 pKa = 4.09 KK269 pKa = 11.21 ANRR272 pKa = 11.84 VAPNLADD279 pKa = 3.99 FSEE282 pKa = 4.52 SQVYY286 pKa = 10.26 DD287 pKa = 3.42 VVKK290 pKa = 10.76 RR291 pKa = 11.84 ILEE294 pKa = 4.0 EE295 pKa = 3.95 SRR297 pKa = 11.84 VAGGRR302 pKa = 11.84 PARR305 pKa = 11.84 IEE307 pKa = 3.89 WDD309 pKa = 3.49 RR310 pKa = 11.84 HH311 pKa = 3.5 WAEE314 pKa = 3.55 RR315 pKa = 11.84 WLWAPTGTFHH325 pKa = 6.99 SQYY328 pKa = 11.4 DD329 pKa = 3.48 EE330 pKa = 4.28 DD331 pKa = 4.38 QEE333 pKa = 4.68 FKK335 pKa = 11.03 AGCRR339 pKa = 11.84 TLNSKK344 pKa = 10.16 FYY346 pKa = 10.53 ALSRR350 pKa = 11.84 MPDD353 pKa = 2.83 VKK355 pKa = 11.04 YY356 pKa = 9.67 EE357 pKa = 3.99 HH358 pKa = 7.09 FYY360 pKa = 11.23 NRR362 pKa = 11.84 PPTCKK367 pKa = 9.8 ARR369 pKa = 11.84 ASVKK373 pKa = 10.32 YY374 pKa = 9.53 EE375 pKa = 3.5 WGKK378 pKa = 9.81 QRR380 pKa = 11.84 AIYY383 pKa = 9.83 GVDD386 pKa = 3.11 VTNFIISGFAFKK398 pKa = 10.73 GCEE401 pKa = 3.63 EE402 pKa = 5.3 AISKK406 pKa = 9.95 IYY408 pKa = 10.05 PIGLEE413 pKa = 3.82 AKK415 pKa = 8.38 EE416 pKa = 4.23 EE417 pKa = 3.99 KK418 pKa = 10.31 VAAHH422 pKa = 5.23 VRR424 pKa = 11.84 EE425 pKa = 4.07 VLKK428 pKa = 11.07 DD429 pKa = 3.5 GVPFCFDD436 pKa = 3.42 FEE438 pKa = 5.02 DD439 pKa = 4.7 FNSQHH444 pKa = 6.43 SLGSMRR450 pKa = 11.84 AVMLAYY456 pKa = 9.87 KK457 pKa = 9.23 NTFEE461 pKa = 5.52 HH462 pKa = 7.68 DD463 pKa = 3.18 ITPEE467 pKa = 3.74 QLRR470 pKa = 11.84 GIDD473 pKa = 3.45 WMIEE477 pKa = 4.09 SLRR480 pKa = 11.84 NTQVVSPAGEE490 pKa = 3.79 FSIVDD495 pKa = 3.58 TLMSGSRR502 pKa = 11.84 LTTFMNTVLNDD513 pKa = 3.42 AYY515 pKa = 11.26 VRR517 pKa = 11.84 LLWRR521 pKa = 11.84 GRR523 pKa = 11.84 VTSTLHH529 pKa = 6.69 NGDD532 pKa = 4.34 DD533 pKa = 3.54 VLAAVTTYY541 pKa = 10.79 EE542 pKa = 4.49 QVQQLMAGAIDD553 pKa = 3.67 HH554 pKa = 6.14 NVRR557 pKa = 11.84 FQMSKK562 pKa = 9.94 CHH564 pKa = 5.89 LASVAEE570 pKa = 4.23 FLRR573 pKa = 11.84 VDD575 pKa = 3.65 HH576 pKa = 6.27 MAGTGTQYY584 pKa = 11.19 LARR587 pKa = 11.84 ACATLVHH594 pKa = 6.67 GPTDD598 pKa = 3.64 ALDD601 pKa = 3.9 PRR603 pKa = 11.84 DD604 pKa = 3.83 PVQQLSAMRR613 pKa = 11.84 VRR615 pKa = 11.84 AEE617 pKa = 3.54 EE618 pKa = 4.48 AIARR622 pKa = 11.84 GAARR626 pKa = 11.84 AEE628 pKa = 4.24 VEE630 pKa = 4.1 KK631 pKa = 10.13 LTTVLRR637 pKa = 11.84 EE638 pKa = 3.98 SVEE641 pKa = 3.8 RR642 pKa = 11.84 RR643 pKa = 11.84 FNIGFDD649 pKa = 3.34 TRR651 pKa = 11.84 ILEE654 pKa = 4.3 RR655 pKa = 11.84 VHH657 pKa = 6.22 VSRR660 pKa = 11.84 GGYY663 pKa = 9.08 SEE665 pKa = 5.13 VVDD668 pKa = 4.28 EE669 pKa = 4.41 EE670 pKa = 4.57 TLSKK674 pKa = 10.72 EE675 pKa = 4.0 VTKK678 pKa = 11.25 CEE680 pKa = 4.11 VTVNSYY686 pKa = 10.71 GDD688 pKa = 3.53 KK689 pKa = 10.94 AVVDD693 pKa = 3.64 MTKK696 pKa = 10.46 RR697 pKa = 11.84 FPGVDD702 pKa = 2.97 AYY704 pKa = 11.03 ARR706 pKa = 11.84 LVTKK710 pKa = 10.37 KK711 pKa = 10.76 YY712 pKa = 10.69 GIEE715 pKa = 3.83 QEE717 pKa = 4.3 YY718 pKa = 7.51 QTIRR722 pKa = 11.84 GNMKK726 pKa = 10.04 SAIGRR731 pKa = 11.84 AAIEE735 pKa = 4.12 GGIGVDD741 pKa = 3.76 VRR743 pKa = 11.84 DD744 pKa = 3.79 VTPDD748 pKa = 3.27 HH749 pKa = 6.49 LAVLAAHH756 pKa = 6.59 KK757 pKa = 10.03 RR758 pKa = 11.84 KK759 pKa = 10.02 AYY761 pKa = 9.3 SHH763 pKa = 6.19 VRR765 pKa = 11.84 LSLKK769 pKa = 10.9 ANIARR774 pKa = 11.84 AFGIPILLGAEE785 pKa = 4.05 YY786 pKa = 9.74 GQQYY790 pKa = 10.73 NVLLYY795 pKa = 11.11 GSVDD799 pKa = 3.04 KK800 pKa = 11.43 LKK802 pKa = 10.9 ALRR805 pKa = 11.84 AWFF808 pKa = 3.82
Molecular weight: 91.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.417
IPC2_protein 7.805
IPC_protein 7.688
Toseland 7.571
ProMoST 8.2
Dawson 8.404
Bjellqvist 8.624
Wikipedia 8.39
Rodwell 8.448
Grimsley 7.658
Solomon 8.492
Lehninger 8.492
Nozaki 8.682
DTASelect 8.434
Thurlkill 8.478
EMBOSS 8.595
Sillero 8.741
Patrickios 4.469
IPC_peptide 8.478
IPC2_peptide 7.512
IPC2.peptide.svr19 7.718
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1619
808
811
809.5
90.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.771 ± 0.174
1.235 ± 0.161
5.312 ± 0.169
6.485 ± 0.033
3.83 ± 0.33
6.485 ± 0.114
2.285 ± 0.125
4.2 ± 0.076
4.015 ± 0.29
9.265 ± 0.174
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.347 ± 0.003
4.509 ± 0.849
3.521 ± 0.036
2.718 ± 0.166
7.288 ± 0.66
7.103 ± 0.439
5.683 ± 0.088
9.265 ± 0.477
1.853 ± 0.079
3.83 ± 0.086
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here