Tomato mottle mosaic virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Virgaviridae; Tobamovirus

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T1WEZ3|T1WEZ3_9VIRU Capsid protein OS=Tomato mottle mosaic virus OX=1391702 GN=CP PE=3 SV=1
MM1 pKa = 7.66SYY3 pKa = 10.99AITSPSQFVFLSSAWADD20 pKa = 3.29PVEE23 pKa = 5.21LINICTNSLGNQFQTQQARR42 pKa = 11.84TTVQQQFSEE51 pKa = 4.29VWKK54 pKa = 9.34PFPQSTVRR62 pKa = 11.84FPDD65 pKa = 3.24NVFKK69 pKa = 10.86VYY71 pKa = 10.34RR72 pKa = 11.84YY73 pKa = 9.93NAVIDD78 pKa = 4.13PLITALLGTFDD89 pKa = 3.24TRR91 pKa = 11.84NRR93 pKa = 11.84IIEE96 pKa = 4.17VEE98 pKa = 4.14NQQSPTTAEE107 pKa = 3.94TLDD110 pKa = 3.11ATRR113 pKa = 11.84RR114 pKa = 11.84VDD116 pKa = 3.5DD117 pKa = 3.56ATVAIRR123 pKa = 11.84SAVNNLVNEE132 pKa = 4.59LVRR135 pKa = 11.84GTGFYY140 pKa = 10.4NQSTFEE146 pKa = 4.24SMSGLAWTSAPASS159 pKa = 3.64

Molecular weight: 17.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T1WEP4|T1WEP4_9VIRU Methyltransferase/RNA helicase OS=Tomato mottle mosaic virus OX=1391702 PE=4 SV=1
MM1 pKa = 7.68ALTVSGKK8 pKa = 9.44VRR10 pKa = 11.84ISEE13 pKa = 4.76FIDD16 pKa = 3.58LSKK19 pKa = 10.87SEE21 pKa = 4.5RR22 pKa = 11.84LLPSMFTHH30 pKa = 6.09VKK32 pKa = 9.38SVSVSKK38 pKa = 10.72VDD40 pKa = 3.01KK41 pKa = 11.32VMVNEE46 pKa = 4.12EE47 pKa = 4.31DD48 pKa = 3.7SLSEE52 pKa = 4.08VNLLKK57 pKa = 10.54GVKK60 pKa = 9.87LIDD63 pKa = 3.69GGYY66 pKa = 8.67VCLAGLVVSGEE77 pKa = 3.84WNLPDD82 pKa = 3.43NCRR85 pKa = 11.84GGVSICLVDD94 pKa = 4.74KK95 pKa = 10.99RR96 pKa = 11.84MQRR99 pKa = 11.84ADD101 pKa = 3.35EE102 pKa = 4.34ATLGSYY108 pKa = 7.82YY109 pKa = 10.45TGAAKK114 pKa = 10.18KK115 pKa = 9.96RR116 pKa = 11.84FQFKK120 pKa = 10.0IVPNYY125 pKa = 10.84AITTKK130 pKa = 10.61DD131 pKa = 2.95AEE133 pKa = 4.29RR134 pKa = 11.84NIWQVLVNIRR144 pKa = 11.84NVKK147 pKa = 8.31MAGGFCPLSLEE158 pKa = 4.22FVSVCIVYY166 pKa = 10.57KK167 pKa = 11.11NNIKK171 pKa = 10.61LGLRR175 pKa = 11.84EE176 pKa = 4.37KK177 pKa = 9.58ITRR180 pKa = 11.84VDD182 pKa = 3.31DD183 pKa = 4.44AGPIEE188 pKa = 4.28LTEE191 pKa = 4.08EE192 pKa = 4.25VVDD195 pKa = 4.03EE196 pKa = 4.6FMEE199 pKa = 4.38SVPMSVRR206 pKa = 11.84LAKK209 pKa = 10.42FRR211 pKa = 11.84TKK213 pKa = 10.38SSKK216 pKa = 10.35RR217 pKa = 11.84GPKK220 pKa = 9.83HH221 pKa = 6.05NSNNTNEE228 pKa = 4.23RR229 pKa = 11.84KK230 pKa = 9.91GRR232 pKa = 11.84SNFRR236 pKa = 11.84KK237 pKa = 10.11KK238 pKa = 10.07QDD240 pKa = 3.33QEE242 pKa = 4.68SYY244 pKa = 11.0GVSDD248 pKa = 3.99SLDD251 pKa = 3.41NLIEE255 pKa = 5.46DD256 pKa = 4.03DD257 pKa = 4.44TEE259 pKa = 4.3TSVAGSDD266 pKa = 3.75SYY268 pKa = 12.17

Molecular weight: 29.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3159

159

1616

789.8

89.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.648 ± 0.456

1.773 ± 0.179

6.046 ± 0.304

6.299 ± 0.342

4.843 ± 0.337

4.4 ± 0.438

2.089 ± 0.46

4.78 ± 0.064

6.394 ± 0.794

9.528 ± 0.618

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.722 ± 0.225

4.179 ± 0.607

3.292 ± 0.262

3.45 ± 0.602

5.192 ± 0.243

8.452 ± 0.419

6.141 ± 0.679

9.022 ± 0.457

0.855 ± 0.154

3.862 ± 0.359
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski