Xenorhabdus stockiae
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3997 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D0KN34|A0A2D0KN34_9GAMM Bifunctional L-3-phosphoserine phosphatase/1-acyl-sn-glycerol-3-phosphate acyltransferase OS=Xenorhabdus stockiae OX=351614 GN=Xsto_02682 PE=4 SV=1
MM1 pKa = 7.12 MKK3 pKa = 9.99 IGLFYY8 pKa = 11.05 GSTTCYY14 pKa = 10.43 TEE16 pKa = 3.93 MVAEE20 pKa = 5.25 KK21 pKa = 10.28 IRR23 pKa = 11.84 DD24 pKa = 3.53 ILGEE28 pKa = 4.04 DD29 pKa = 5.01 LIDD32 pKa = 3.48 LHH34 pKa = 6.43 NVKK37 pKa = 10.72 DD38 pKa = 4.31 CDD40 pKa = 3.69 PQLMEE45 pKa = 4.92 EE46 pKa = 4.33 YY47 pKa = 10.45 SVLILGMPTWDD58 pKa = 3.18 FGEE61 pKa = 4.25 IQEE64 pKa = 4.63 DD65 pKa = 3.61 WLAIWDD71 pKa = 4.07 QLPSLDD77 pKa = 4.08 LSGKK81 pKa = 8.6 IVAMYY86 pKa = 11.06 GMGDD90 pKa = 3.51 QIDD93 pKa = 3.87 YY94 pKa = 11.36 SEE96 pKa = 4.5 WFLDD100 pKa = 3.63 ALGMLYY106 pKa = 10.07 HH107 pKa = 6.86 HH108 pKa = 7.44 LLPTGAQFIGFWPTEE123 pKa = 4.12 GYY125 pKa = 10.67 EE126 pKa = 4.26 FTSPKK131 pKa = 10.28 PLTDD135 pKa = 4.0 DD136 pKa = 3.82 GKK138 pKa = 10.82 QFVGLALDD146 pKa = 4.25 DD147 pKa = 4.02 VNQFEE152 pKa = 4.6 EE153 pKa = 4.08 TDD155 pKa = 3.32 EE156 pKa = 5.76 RR157 pKa = 11.84 LSQWCMQILAEE168 pKa = 4.2 MEE170 pKa = 4.08 ALFF173 pKa = 5.17
Molecular weight: 19.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.714
IPC2_protein 3.834
IPC_protein 3.821
Toseland 3.617
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.719
Rodwell 3.656
Grimsley 3.528
Solomon 3.795
Lehninger 3.745
Nozaki 3.923
DTASelect 4.126
Thurlkill 3.668
EMBOSS 3.732
Sillero 3.935
Patrickios 1.062
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.837
Protein with the highest isoelectric point:
>tr|A0A2D0KS31|A0A2D0KS31_9GAMM Uncharacterized protein OS=Xenorhabdus stockiae OX=351614 GN=Xsto_01350 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.68 RR12 pKa = 11.84 NRR14 pKa = 11.84 THH16 pKa = 7.28 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.22 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LTVSKK46 pKa = 10.99
Molecular weight: 5.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3997
0
3997
1305138
29
11358
326.5
36.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.136 ± 0.039
1.067 ± 0.015
5.159 ± 0.031
6.057 ± 0.038
3.987 ± 0.029
6.862 ± 0.043
2.371 ± 0.022
6.93 ± 0.04
5.094 ± 0.058
10.55 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.511 ± 0.022
4.486 ± 0.032
4.267 ± 0.036
4.681 ± 0.043
5.086 ± 0.031
6.474 ± 0.034
5.386 ± 0.031
6.33 ± 0.029
1.348 ± 0.016
3.22 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here