Streptococcus phage Javan199

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A497|A0A4D6A497_9CAUD Uncharacterized protein OS=Streptococcus phage Javan199 OX=2548043 GN=Javan199_0039 PE=4 SV=1
MM1 pKa = 7.07TPKK4 pKa = 10.26INFGKK9 pKa = 9.57WYY11 pKa = 9.06EE12 pKa = 4.09DD13 pKa = 3.71EE14 pKa = 5.57EE15 pKa = 4.97IDD17 pKa = 5.09SVFIDD22 pKa = 3.65FTGISGGYY30 pKa = 8.88CGYY33 pKa = 11.06EE34 pKa = 4.77DD35 pKa = 4.18IPNDD39 pKa = 3.09IRR41 pKa = 11.84TMIFTAQQGDD51 pKa = 3.75GEE53 pKa = 4.61GPYY56 pKa = 10.72NYY58 pKa = 10.09VEE60 pKa = 4.55VINPEE65 pKa = 3.77PFLNSKK71 pKa = 10.18FADD74 pKa = 3.57SYY76 pKa = 11.33EE77 pKa = 4.18LNGYY81 pKa = 8.41NIKK84 pKa = 10.29QLILDD89 pKa = 5.1AYY91 pKa = 9.68NKK93 pKa = 10.12QNIAA97 pKa = 3.51

Molecular weight: 11.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A2S1|A0A4D6A2S1_9CAUD Uncharacterized protein OS=Streptococcus phage Javan199 OX=2548043 GN=Javan199_0018 PE=4 SV=1
MM1 pKa = 6.95ATNRR5 pKa = 11.84EE6 pKa = 4.01LDD8 pKa = 3.61LSSKK12 pKa = 10.36RR13 pKa = 11.84IEE15 pKa = 3.99RR16 pKa = 11.84MAKK19 pKa = 9.28VKK21 pKa = 10.99APVDD25 pKa = 3.34TGALKK30 pKa = 9.19NTIFSAKK37 pKa = 10.06AGNLTYY43 pKa = 10.75KK44 pKa = 9.03VTAPQHH50 pKa = 4.35YY51 pKa = 9.9AIYY54 pKa = 10.09VEE56 pKa = 4.0KK57 pKa = 9.85GTRR60 pKa = 11.84KK61 pKa = 8.61MRR63 pKa = 11.84AQPYY67 pKa = 9.44LKK69 pKa = 9.87PALDD73 pKa = 3.64AEE75 pKa = 4.36RR76 pKa = 11.84PKK78 pKa = 10.9LISNLRR84 pKa = 11.84KK85 pKa = 9.88LYY87 pKa = 10.4EE88 pKa = 3.86RR89 pKa = 4.77

Molecular weight: 10.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

12434

39

2527

253.8

28.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.032 ± 0.996

0.386 ± 0.092

6.346 ± 0.49

6.587 ± 0.77

3.426 ± 0.316

5.992 ± 0.393

1.23 ± 0.192

6.354 ± 0.224

7.447 ± 0.487

7.922 ± 0.268

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.268 ± 0.394

6.233 ± 0.342

2.726 ± 0.459

5.051 ± 0.305

3.796 ± 0.492

6.804 ± 1.001

7.705 ± 1.141

6.072 ± 0.194

1.078 ± 0.165

3.547 ± 0.453
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski