Amino acid dipepetide frequency for Streptococcus phage Javan199

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.193AlaAla: 6.193 ± 2.076
0.08AlaCys: 0.08 ± 0.098
6.274AlaAsp: 6.274 ± 0.875
7.319AlaGlu: 7.319 ± 1.184
1.93AlaPhe: 1.93 ± 0.618
6.515AlaGly: 6.515 ± 1.718
0.965AlaHis: 0.965 ± 0.351
5.952AlaIle: 5.952 ± 0.698
6.837AlaLys: 6.837 ± 0.701
5.952AlaLeu: 5.952 ± 0.845
2.252AlaMet: 2.252 ± 0.955
6.998AlaAsn: 6.998 ± 1.701
2.172AlaPro: 2.172 ± 0.605
4.826AlaGln: 4.826 ± 1.068
2.654AlaArg: 2.654 ± 0.385
6.676AlaSer: 6.676 ± 1.745
6.917AlaThr: 6.917 ± 1.504
6.595AlaVal: 6.595 ± 0.981
1.287AlaTrp: 1.287 ± 0.354
1.93AlaTyr: 1.93 ± 0.739
0.0AlaXaa: 0.0 ± 0.0
Cys
0.161CysAla: 0.161 ± 0.137
0.08CysCys: 0.08 ± 0.1
0.161CysAsp: 0.161 ± 0.118
0.402CysGlu: 0.402 ± 0.227
0.241CysPhe: 0.241 ± 0.134
0.241CysGly: 0.241 ± 0.153
0.08CysHis: 0.08 ± 0.079
0.322CysIle: 0.322 ± 0.194
0.402CysLys: 0.402 ± 0.208
0.0CysLeu: 0.0 ± 0.0
0.241CysMet: 0.241 ± 0.15
0.322CysAsn: 0.322 ± 0.157
0.161CysPro: 0.161 ± 0.116
0.322CysGln: 0.322 ± 0.156
0.322CysArg: 0.322 ± 0.183
0.241CysSer: 0.241 ± 0.135
0.0CysThr: 0.0 ± 0.0
0.161CysVal: 0.161 ± 0.128
0.0CysTrp: 0.0 ± 0.0
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
4.424AspAla: 4.424 ± 0.505
0.241AspCys: 0.241 ± 0.182
3.861AspAsp: 3.861 ± 0.843
5.791AspGlu: 5.791 ± 0.957
2.976AspPhe: 2.976 ± 0.664
4.826AspGly: 4.826 ± 1.164
0.563AspHis: 0.563 ± 0.231
3.861AspIle: 3.861 ± 0.624
4.343AspLys: 4.343 ± 0.679
5.469AspLeu: 5.469 ± 0.92
0.965AspMet: 0.965 ± 0.317
4.665AspAsn: 4.665 ± 0.9
1.367AspPro: 1.367 ± 0.45
1.769AspGln: 1.769 ± 0.417
2.252AspArg: 2.252 ± 0.589
3.539AspSer: 3.539 ± 0.446
3.861AspThr: 3.861 ± 0.609
3.941AspVal: 3.941 ± 0.528
0.643AspTrp: 0.643 ± 0.216
4.102AspTyr: 4.102 ± 0.682
0.0AspXaa: 0.0 ± 0.0
Glu
4.022GluAla: 4.022 ± 0.92
0.402GluCys: 0.402 ± 0.251
5.469GluAsp: 5.469 ± 0.906
4.424GluGlu: 4.424 ± 1.106
2.333GluPhe: 2.333 ± 0.664
3.539GluGly: 3.539 ± 0.783
0.563GluHis: 0.563 ± 0.237
3.78GluIle: 3.78 ± 0.745
3.619GluLys: 3.619 ± 0.613
8.928GluLeu: 8.928 ± 1.07
2.011GluMet: 2.011 ± 0.708
3.056GluAsn: 3.056 ± 0.628
2.252GluPro: 2.252 ± 0.635
3.459GluGln: 3.459 ± 0.879
3.861GluArg: 3.861 ± 0.838
3.539GluSer: 3.539 ± 0.668
4.022GluThr: 4.022 ± 0.477
3.78GluVal: 3.78 ± 0.661
0.804GluTrp: 0.804 ± 0.338
2.011GluTyr: 2.011 ± 0.422
0.0GluXaa: 0.0 ± 0.0
Phe
2.654PheAla: 2.654 ± 0.761
0.08PheCys: 0.08 ± 0.089
2.815PheAsp: 2.815 ± 0.558
1.93PheGlu: 1.93 ± 0.399
1.046PhePhe: 1.046 ± 0.362
2.735PheGly: 2.735 ± 0.417
0.483PheHis: 0.483 ± 0.168
2.252PheIle: 2.252 ± 0.5
2.413PheLys: 2.413 ± 0.765
2.413PheLeu: 2.413 ± 0.546
0.724PheMet: 0.724 ± 0.341
2.896PheAsn: 2.896 ± 0.536
0.643PhePro: 0.643 ± 0.215
1.609PheGln: 1.609 ± 0.596
0.885PheArg: 0.885 ± 0.272
2.413PheSer: 2.413 ± 0.466
2.413PheThr: 2.413 ± 0.4
1.85PheVal: 1.85 ± 0.584
0.241PheTrp: 0.241 ± 0.111
1.769PheTyr: 1.769 ± 0.579
0.0PheXaa: 0.0 ± 0.0
Gly
5.469GlyAla: 5.469 ± 1.216
0.241GlyCys: 0.241 ± 0.109
3.217GlyAsp: 3.217 ± 0.508
3.378GlyGlu: 3.378 ± 0.459
2.574GlyPhe: 2.574 ± 0.458
2.815GlyGly: 2.815 ± 0.409
0.563GlyHis: 0.563 ± 0.272
4.504GlyIle: 4.504 ± 0.714
5.791GlyLys: 5.791 ± 0.884
4.585GlyLeu: 4.585 ± 1.064
2.172GlyMet: 2.172 ± 0.824
3.137GlyAsn: 3.137 ± 0.441
0.724GlyPro: 0.724 ± 0.268
2.574GlyGln: 2.574 ± 0.594
2.333GlyArg: 2.333 ± 0.564
4.343GlySer: 4.343 ± 0.67
3.941GlyThr: 3.941 ± 0.707
4.263GlyVal: 4.263 ± 0.702
1.126GlyTrp: 1.126 ± 0.328
2.172GlyTyr: 2.172 ± 0.484
0.0GlyXaa: 0.0 ± 0.0
His
1.046HisAla: 1.046 ± 0.335
0.08HisCys: 0.08 ± 0.108
0.643HisAsp: 0.643 ± 0.215
1.126HisGlu: 1.126 ± 0.356
0.563HisPhe: 0.563 ± 0.209
0.483HisGly: 0.483 ± 0.143
0.563HisHis: 0.563 ± 0.256
0.965HisIle: 0.965 ± 0.289
0.643HisLys: 0.643 ± 0.249
0.804HisLeu: 0.804 ± 0.303
0.161HisMet: 0.161 ± 0.097
0.643HisAsn: 0.643 ± 0.248
0.322HisPro: 0.322 ± 0.183
0.483HisGln: 0.483 ± 0.196
0.402HisArg: 0.402 ± 0.204
0.965HisSer: 0.965 ± 0.407
1.367HisThr: 1.367 ± 0.371
0.322HisVal: 0.322 ± 0.16
0.0HisTrp: 0.0 ± 0.0
0.724HisTyr: 0.724 ± 0.25
0.0HisXaa: 0.0 ± 0.0
Ile
6.354IleAla: 6.354 ± 0.81
0.643IleCys: 0.643 ± 0.274
5.148IleAsp: 5.148 ± 1.088
4.906IleGlu: 4.906 ± 0.767
1.689IlePhe: 1.689 ± 0.493
3.459IleGly: 3.459 ± 0.576
0.322IleHis: 0.322 ± 0.139
3.78IleIle: 3.78 ± 0.598
4.987IleLys: 4.987 ± 0.637
3.539IleLeu: 3.539 ± 0.567
1.126IleMet: 1.126 ± 0.273
4.022IleAsn: 4.022 ± 0.646
2.252IlePro: 2.252 ± 0.383
3.298IleGln: 3.298 ± 0.66
2.735IleArg: 2.735 ± 0.459
4.182IleSer: 4.182 ± 0.917
4.906IleThr: 4.906 ± 1.133
3.137IleVal: 3.137 ± 0.595
0.241IleTrp: 0.241 ± 0.16
2.815IleTyr: 2.815 ± 0.835
0.0IleXaa: 0.0 ± 0.0
Lys
8.284LysAla: 8.284 ± 0.76
0.161LysCys: 0.161 ± 0.127
3.056LysAsp: 3.056 ± 0.611
4.424LysGlu: 4.424 ± 0.947
2.333LysPhe: 2.333 ± 0.438
4.263LysGly: 4.263 ± 0.42
1.126LysHis: 1.126 ± 0.368
5.63LysIle: 5.63 ± 0.668
5.389LysLys: 5.389 ± 1.302
5.389LysLeu: 5.389 ± 0.783
1.689LysMet: 1.689 ± 0.315
4.182LysAsn: 4.182 ± 0.636
2.091LysPro: 2.091 ± 0.52
3.298LysGln: 3.298 ± 0.582
3.78LysArg: 3.78 ± 0.989
4.504LysSer: 4.504 ± 0.671
6.274LysThr: 6.274 ± 1.757
4.665LysVal: 4.665 ± 0.531
1.448LysTrp: 1.448 ± 0.33
2.493LysTyr: 2.493 ± 0.595
0.0LysXaa: 0.0 ± 0.0
Leu
6.917LeuAla: 6.917 ± 0.711
0.161LeuCys: 0.161 ± 0.171
6.354LeuAsp: 6.354 ± 1.015
5.389LeuGlu: 5.389 ± 1.089
2.815LeuPhe: 2.815 ± 0.711
4.424LeuGly: 4.424 ± 0.938
1.206LeuHis: 1.206 ± 0.375
5.067LeuIle: 5.067 ± 0.752
6.676LeuLys: 6.676 ± 0.784
4.826LeuLeu: 4.826 ± 1.036
1.287LeuMet: 1.287 ± 0.439
5.067LeuAsn: 5.067 ± 0.735
2.735LeuPro: 2.735 ± 0.383
4.022LeuGln: 4.022 ± 0.621
2.815LeuArg: 2.815 ± 0.489
6.756LeuSer: 6.756 ± 1.172
7.4LeuThr: 7.4 ± 1.626
3.217LeuVal: 3.217 ± 0.518
0.402LeuTrp: 0.402 ± 0.171
1.689LeuTyr: 1.689 ± 0.325
0.0LeuXaa: 0.0 ± 0.0
Met
3.056MetAla: 3.056 ± 1.182
0.0MetCys: 0.0 ± 0.0
1.367MetAsp: 1.367 ± 0.506
0.724MetGlu: 0.724 ± 0.249
0.643MetPhe: 0.643 ± 0.296
0.965MetGly: 0.965 ± 0.285
0.08MetHis: 0.08 ± 0.079
1.287MetIle: 1.287 ± 0.398
2.252MetLys: 2.252 ± 0.558
1.287MetLeu: 1.287 ± 0.42
0.08MetMet: 0.08 ± 0.092
1.287MetAsn: 1.287 ± 0.538
1.126MetPro: 1.126 ± 0.29
1.367MetGln: 1.367 ± 0.472
1.046MetArg: 1.046 ± 0.454
1.689MetSer: 1.689 ± 0.657
1.93MetThr: 1.93 ± 0.411
1.609MetVal: 1.609 ± 0.389
0.322MetTrp: 0.322 ± 0.153
0.563MetTyr: 0.563 ± 0.194
0.0MetXaa: 0.0 ± 0.0
Asn
5.711AsnAla: 5.711 ± 1.53
0.161AsnCys: 0.161 ± 0.088
3.7AsnAsp: 3.7 ± 0.621
2.976AsnGlu: 2.976 ± 0.564
1.689AsnPhe: 1.689 ± 0.334
5.067AsnGly: 5.067 ± 0.816
0.804AsnHis: 0.804 ± 0.275
3.861AsnIle: 3.861 ± 0.61
3.619AsnLys: 3.619 ± 0.578
6.193AsnLeu: 6.193 ± 0.933
2.011AsnMet: 2.011 ± 0.478
3.619AsnAsn: 3.619 ± 0.613
1.85AsnPro: 1.85 ± 0.784
3.217AsnGln: 3.217 ± 0.586
2.493AsnArg: 2.493 ± 0.608
4.182AsnSer: 4.182 ± 0.725
3.459AsnThr: 3.459 ± 0.926
3.941AsnVal: 3.941 ± 0.561
1.046AsnTrp: 1.046 ± 0.317
2.735AsnTyr: 2.735 ± 0.474
0.0AsnXaa: 0.0 ± 0.0
Pro
3.056ProAla: 3.056 ± 0.721
0.161ProCys: 0.161 ± 0.097
1.93ProAsp: 1.93 ± 0.465
2.735ProGlu: 2.735 ± 0.79
1.046ProPhe: 1.046 ± 0.378
1.206ProGly: 1.206 ± 0.287
0.08ProHis: 0.08 ± 0.089
1.769ProIle: 1.769 ± 0.397
2.091ProLys: 2.091 ± 0.699
2.252ProLeu: 2.252 ± 0.492
0.322ProMet: 0.322 ± 0.177
1.528ProAsn: 1.528 ± 0.536
0.724ProPro: 0.724 ± 0.319
2.011ProGln: 2.011 ± 0.938
0.724ProArg: 0.724 ± 0.268
1.206ProSer: 1.206 ± 0.334
1.93ProThr: 1.93 ± 0.515
1.367ProVal: 1.367 ± 0.421
0.08ProTrp: 0.08 ± 0.086
1.046ProTyr: 1.046 ± 0.3
0.0ProXaa: 0.0 ± 0.0
Gln
5.952GlnAla: 5.952 ± 0.857
0.483GlnCys: 0.483 ± 0.191
1.287GlnAsp: 1.287 ± 0.288
2.172GlnGlu: 2.172 ± 0.642
2.333GlnPhe: 2.333 ± 0.487
2.654GlnGly: 2.654 ± 0.458
0.885GlnHis: 0.885 ± 0.278
4.504GlnIle: 4.504 ± 0.933
3.378GlnLys: 3.378 ± 0.594
3.7GlnLeu: 3.7 ± 0.707
1.126GlnMet: 1.126 ± 0.351
2.896GlnAsn: 2.896 ± 0.61
1.93GlnPro: 1.93 ± 0.699
3.378GlnGln: 3.378 ± 1.159
2.172GlnArg: 2.172 ± 0.502
3.137GlnSer: 3.137 ± 0.72
4.987GlnThr: 4.987 ± 1.076
1.448GlnVal: 1.448 ± 0.405
0.322GlnTrp: 0.322 ± 0.16
1.769GlnTyr: 1.769 ± 0.384
0.0GlnXaa: 0.0 ± 0.0
Arg
2.896ArgAla: 2.896 ± 0.366
0.08ArgCys: 0.08 ± 0.096
1.85ArgAsp: 1.85 ± 0.54
2.333ArgGlu: 2.333 ± 0.502
1.046ArgPhe: 1.046 ± 0.35
2.011ArgGly: 2.011 ± 0.592
0.965ArgHis: 0.965 ± 0.402
2.333ArgIle: 2.333 ± 0.392
2.976ArgLys: 2.976 ± 0.896
4.022ArgLeu: 4.022 ± 0.894
1.287ArgMet: 1.287 ± 0.423
1.93ArgAsn: 1.93 ± 0.403
0.724ArgPro: 0.724 ± 0.338
1.367ArgGln: 1.367 ± 0.286
1.528ArgArg: 1.528 ± 0.325
1.93ArgSer: 1.93 ± 0.589
3.217ArgThr: 3.217 ± 0.587
3.056ArgVal: 3.056 ± 0.544
0.724ArgTrp: 0.724 ± 0.367
1.689ArgTyr: 1.689 ± 0.587
0.0ArgXaa: 0.0 ± 0.0
Ser
6.595SerAla: 6.595 ± 2.142
0.0SerCys: 0.0 ± 0.0
4.343SerAsp: 4.343 ± 0.723
3.619SerGlu: 3.619 ± 0.682
2.091SerPhe: 2.091 ± 0.576
4.745SerGly: 4.745 ± 0.924
0.643SerHis: 0.643 ± 0.253
4.022SerIle: 4.022 ± 0.642
4.182SerLys: 4.182 ± 0.67
6.515SerLeu: 6.515 ± 1.497
1.85SerMet: 1.85 ± 0.521
4.022SerAsn: 4.022 ± 0.984
1.287SerPro: 1.287 ± 0.247
3.298SerGln: 3.298 ± 0.588
1.769SerArg: 1.769 ± 0.405
5.148SerSer: 5.148 ± 1.633
5.63SerThr: 5.63 ± 1.225
5.308SerVal: 5.308 ± 1.045
0.724SerTrp: 0.724 ± 0.21
2.252SerTyr: 2.252 ± 0.424
0.0SerXaa: 0.0 ± 0.0
Thr
8.687ThrAla: 8.687 ± 1.794
0.241ThrCys: 0.241 ± 0.135
4.826ThrAsp: 4.826 ± 0.801
4.343ThrGlu: 4.343 ± 0.745
2.896ThrPhe: 2.896 ± 0.406
5.308ThrGly: 5.308 ± 0.647
0.724ThrHis: 0.724 ± 0.247
4.263ThrIle: 4.263 ± 0.485
6.032ThrLys: 6.032 ± 1.558
5.308ThrLeu: 5.308 ± 0.782
1.689ThrMet: 1.689 ± 0.343
5.55ThrAsn: 5.55 ± 0.717
1.93ThrPro: 1.93 ± 0.549
3.298ThrGln: 3.298 ± 0.538
1.85ThrArg: 1.85 ± 0.422
6.595ThrSer: 6.595 ± 2.656
5.711ThrThr: 5.711 ± 1.841
5.148ThrVal: 5.148 ± 1.599
0.322ThrTrp: 0.322 ± 0.182
2.333ThrTyr: 2.333 ± 0.468
0.0ThrXaa: 0.0 ± 0.0
Val
5.148ValAla: 5.148 ± 0.751
0.322ValCys: 0.322 ± 0.226
3.861ValAsp: 3.861 ± 0.564
4.826ValGlu: 4.826 ± 0.893
2.091ValPhe: 2.091 ± 0.403
2.574ValGly: 2.574 ± 0.461
0.643ValHis: 0.643 ± 0.203
2.574ValIle: 2.574 ± 0.373
5.389ValLys: 5.389 ± 0.604
3.861ValLeu: 3.861 ± 1.029
1.287ValMet: 1.287 ± 0.386
4.182ValAsn: 4.182 ± 0.567
1.85ValPro: 1.85 ± 0.459
3.459ValGln: 3.459 ± 0.833
2.091ValArg: 2.091 ± 0.458
4.263ValSer: 4.263 ± 0.987
5.871ValThr: 5.871 ± 0.653
4.263ValVal: 4.263 ± 0.672
0.563ValTrp: 0.563 ± 0.177
1.609ValTyr: 1.609 ± 0.395
0.0ValXaa: 0.0 ± 0.0
Trp
0.804TrpAla: 0.804 ± 0.369
0.08TrpCys: 0.08 ± 0.079
1.206TrpAsp: 1.206 ± 0.24
0.643TrpGlu: 0.643 ± 0.254
0.322TrpPhe: 0.322 ± 0.165
0.322TrpGly: 0.322 ± 0.167
0.241TrpHis: 0.241 ± 0.167
0.563TrpIle: 0.563 ± 0.273
0.724TrpLys: 0.724 ± 0.296
0.965TrpLeu: 0.965 ± 0.254
0.08TrpMet: 0.08 ± 0.097
0.483TrpAsn: 0.483 ± 0.247
0.08TrpPro: 0.08 ± 0.094
0.965TrpGln: 0.965 ± 0.371
0.724TrpArg: 0.724 ± 0.257
0.724TrpSer: 0.724 ± 0.223
0.563TrpThr: 0.563 ± 0.178
0.885TrpVal: 0.885 ± 0.219
0.161TrpTrp: 0.161 ± 0.104
0.241TrpTyr: 0.241 ± 0.157
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.896TyrAla: 2.896 ± 0.486
0.161TyrCys: 0.161 ± 0.12
2.091TyrAsp: 2.091 ± 0.59
2.413TyrGlu: 2.413 ± 0.848
1.609TyrPhe: 1.609 ± 0.317
1.769TyrGly: 1.769 ± 0.496
0.804TyrHis: 0.804 ± 0.409
2.252TyrIle: 2.252 ± 0.485
2.735TyrLys: 2.735 ± 0.655
3.217TyrLeu: 3.217 ± 0.684
0.241TyrMet: 0.241 ± 0.16
1.85TyrAsn: 1.85 ± 0.553
1.046TyrPro: 1.046 ± 0.342
2.493TyrGln: 2.493 ± 0.529
1.528TyrArg: 1.528 ± 0.531
2.011TyrSer: 2.011 ± 0.349
2.654TyrThr: 2.654 ± 0.55
1.769TyrVal: 1.769 ± 0.385
0.322TyrTrp: 0.322 ± 0.159
1.528TyrTyr: 1.528 ± 0.453
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 49 proteins (12434 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski