Rhizobium phage vB_RglS_P106B

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Rigallicvirus; Rhizobium virus P106B

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W6E8N4|W6E8N4_9CAUD Uncharacterized protein OS=Rhizobium phage vB_RglS_P106B OX=1458697 GN=P106B_61 PE=4 SV=1
MM1 pKa = 6.96KK2 pKa = 9.51TVAEE6 pKa = 4.1IAEE9 pKa = 4.43EE10 pKa = 4.4LRR12 pKa = 11.84GTCQTLTIVLEE23 pKa = 4.29NNDD26 pKa = 4.09MDD28 pKa = 5.79GMDD31 pKa = 4.29NDD33 pKa = 4.25AAFCAEE39 pKa = 4.13LDD41 pKa = 3.81SLVFCCEE48 pKa = 3.75RR49 pKa = 11.84CDD51 pKa = 3.17WWHH54 pKa = 6.16EE55 pKa = 3.86QSEE58 pKa = 4.4MSDD61 pKa = 3.53KK62 pKa = 10.96FEE64 pKa = 3.83AWICEE69 pKa = 3.94EE70 pKa = 4.4CASEE74 pKa = 4.09VHH76 pKa = 6.56

Molecular weight: 8.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W6EKE2|W6EKE2_9CAUD Lysophospholipase L1-like esterase OS=Rhizobium phage vB_RglS_P106B OX=1458697 GN=P106B_13 PE=4 SV=1
MM1 pKa = 8.0DD2 pKa = 5.5RR3 pKa = 11.84GALDD7 pKa = 4.09RR8 pKa = 11.84MIKK11 pKa = 9.47TKK13 pKa = 10.52ISFSTGALAFSPNARR28 pKa = 11.84AANQKK33 pKa = 10.77ALINAA38 pKa = 4.6

Molecular weight: 4.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

17376

38

1172

182.9

20.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.841 ± 0.314

1.007 ± 0.124

5.795 ± 0.207

5.928 ± 0.351

3.856 ± 0.164

7.712 ± 0.377

1.732 ± 0.156

6.371 ± 0.192

5.243 ± 0.267

7.234 ± 0.227

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.624 ± 0.171

5.001 ± 0.182

4.639 ± 0.259

4.023 ± 0.262

5.64 ± 0.305

5.479 ± 0.24

6.474 ± 0.373

6.4 ± 0.3

1.784 ± 0.124

3.217 ± 0.176
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski