Peptidiphaga gingivicola
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1994 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A179B561|A0A179B561_9ACTO BAAT_C domain-containing protein OS=Peptidiphaga gingivicola OX=2741497 GN=A4H34_06905 PE=4 SV=1
MM1 pKa = 6.93 YY2 pKa = 10.46 ASIAASFRR10 pKa = 11.84 AAIFSSILTKK20 pKa = 10.74 SSGVDD25 pKa = 3.3 VEE27 pKa = 4.65 FTMALNTPCGVVSAYY42 pKa = 11.04 SFILLYY48 pKa = 10.44 IFNQVKK54 pKa = 10.35 DD55 pKa = 3.87 VGLGQPPNAEE65 pKa = 4.14 MLDD68 pKa = 3.77 MPLLLIGIVMEE79 pKa = 4.28 STTFSNQDD87 pKa = 2.78 SS88 pKa = 3.72
Molecular weight: 9.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.876
IPC2_protein 4.215
IPC_protein 3.986
Toseland 3.808
ProMoST 4.164
Dawson 3.973
Bjellqvist 4.139
Wikipedia 3.935
Rodwell 3.821
Grimsley 3.732
Solomon 3.948
Lehninger 3.897
Nozaki 4.126
DTASelect 4.291
Thurlkill 3.884
EMBOSS 3.935
Sillero 4.113
Patrickios 1.952
IPC_peptide 3.948
IPC2_peptide 4.075
IPC2.peptide.svr19 4.007
Protein with the highest isoelectric point:
>tr|A0A179B5X1|A0A179B5X1_9ACTO Ribonuclease OS=Peptidiphaga gingivicola OX=2741497 GN=A4H34_06610 PE=4 SV=1
MM1 pKa = 7.27 TALDD5 pKa = 4.01 NLVMTFALPDD15 pKa = 3.37 IKK17 pKa = 10.68 RR18 pKa = 11.84 EE19 pKa = 3.94 LGATVTQLQWFVNAYY34 pKa = 7.34 TLVVASALLPMAALGDD50 pKa = 3.59 RR51 pKa = 11.84 FGRR54 pKa = 11.84 RR55 pKa = 11.84 RR56 pKa = 11.84 IFSYY60 pKa = 10.68 GIAVFAAASAACALAPTPEE79 pKa = 3.93 ALIVARR85 pKa = 11.84 AIQGAGGAAIVTLSLALLVDD105 pKa = 3.92 AVPRR109 pKa = 11.84 RR110 pKa = 11.84 LRR112 pKa = 11.84 EE113 pKa = 3.66 LAIGVWGGVNGLGIAMGPLLGGAVVSGLHH142 pKa = 5.23 WSVVFWINVPIGALSLPLVPRR163 pKa = 11.84 FLPRR167 pKa = 11.84 AAAGSKK173 pKa = 9.19 GADD176 pKa = 3.23 WIGTILGIGFVFPLTWAVVEE196 pKa = 4.67 GPSRR200 pKa = 11.84 GWTDD204 pKa = 2.94 GLTIGGFAVAGTCLVLFLLWEE225 pKa = 4.73 RR226 pKa = 11.84 SAKK229 pKa = 10.63 APIIPLSLFANRR241 pKa = 11.84 RR242 pKa = 11.84 FSLVNAATVLFAAGVFGAIFFLSQFLQITLGYY274 pKa = 9.58 SAFEE278 pKa = 4.29 AGLRR282 pKa = 11.84 AGPWTLLPLFVSPASGGLVKK302 pKa = 10.67 RR303 pKa = 11.84 LGVRR307 pKa = 11.84 RR308 pKa = 11.84 VLVSGMFLQTVALAWFAVKK327 pKa = 10.47 VGADD331 pKa = 3.58 VPYY334 pKa = 9.9 GDD336 pKa = 5.7 CIAPMAVAGLGMGLSFAPLATGALQGVSADD366 pKa = 3.69 RR367 pKa = 11.84 RR368 pKa = 11.84 AVASGVNSTLRR379 pKa = 11.84 HH380 pKa = 5.65 LGVAIGIAACTAIFTAHH397 pKa = 6.32 GKK399 pKa = 9.26 YY400 pKa = 10.57 LPGQPFVDD408 pKa = 5.43 GIKK411 pKa = 10.34 PSLWLCAALLATATVCAEE429 pKa = 4.01 RR430 pKa = 11.84 SDD432 pKa = 3.6 RR433 pKa = 11.84 AARR436 pKa = 11.84 RR437 pKa = 3.49
Molecular weight: 45.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.458
IPC_protein 10.218
Toseland 10.365
ProMoST 10.262
Dawson 10.526
Bjellqvist 10.277
Wikipedia 10.745
Rodwell 10.643
Grimsley 10.584
Solomon 10.613
Lehninger 10.584
Nozaki 10.423
DTASelect 10.248
Thurlkill 10.409
EMBOSS 10.774
Sillero 10.467
Patrickios 10.321
IPC_peptide 10.613
IPC2_peptide 9.56
IPC2.peptide.svr19 8.542
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1994
0
1994
685011
37
2948
343.5
36.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.891 ± 0.085
0.779 ± 0.016
5.93 ± 0.046
6.354 ± 0.06
3.157 ± 0.036
9.249 ± 0.053
1.746 ± 0.021
4.243 ± 0.043
3.593 ± 0.046
9.007 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.038 ± 0.025
2.449 ± 0.031
5.132 ± 0.046
2.709 ± 0.029
7.104 ± 0.064
6.646 ± 0.053
5.202 ± 0.041
8.297 ± 0.052
1.333 ± 0.02
2.139 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here